Understanding how chromosomes fold provides insights into the transcription regulation, hence, the functional state of the cell. Using the next generation sequencing technology, the recently developed Hi-C approach enables a global view... more
Understanding how chromosomes fold provides insights into the transcription regulation, hence, the functional state of the cell. Using the next generation sequencing technology, the recently developed Hi-C approach enables a global view of spatial chromatin organization in the nucleus, which substantially expands our knowledge about genome organization and function. However, due to multiple layers of biases, noises and uncertainties buried in the protocol of Hi-C experiments, analyzing and interpreting Hi-C data poses great challenges, and requires novel statistical methods to be developed. This article provides an overview of recent Hi-C studies and their impacts on biomedical research, describes major challenges in statistical analysis of Hi-C data, and discusses some perspectives for future research.
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To examine and quantify the potential dose-response relationship between red and processed meat consumption and risk of all-cause, cardiovascular and cancer mortality. We searched MEDLINE, Embase, ISI Web of Knowledge, CINHAL, Scopus, the... more
To examine and quantify the potential dose-response relationship between red and processed meat consumption and risk of all-cause, cardiovascular and cancer mortality. We searched MEDLINE, Embase, ISI Web of Knowledge, CINHAL, Scopus, the Cochrane library and reference lists of retrieved articles up to 30 November 2014 without language restrictions. We retrieved prospective cohort studies that reported risk estimates for all-cause, cardiovascular and cancer mortality by red and/or processed meat intake levels. The dose-response relationships were estimated using data from red and processed meat intake categories in each study. Random-effects models were used to calculate pooled relative risks and 95 % confidence intervals and to incorporate between-study variations. Nine articles with seventeen prospective cohorts were eligible in this meta-analysis, including a total of 150 328 deaths. There was evidence of a non-linear association between processed meat consumption and risk of all...
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By employing united atom molecular dynamics simulation, we have investigated the effects of polymer-graphene interaction [Formula: see text] volume fraction of graphene [Formula: see text] thermodynamics of polymer matrix (rubbery versus... more
By employing united atom molecular dynamics simulation, we have investigated the effects of polymer-graphene interaction [Formula: see text] volume fraction of graphene [Formula: see text] thermodynamics of polymer matrix (rubbery versus glassy), interfacial interaction in the case of the same dispersion state, shape of nanoparticles (NPs) such as [Formula: see text] CNT and graphene at the same loading on the toughening efficiency of polymer nanocomposites. By beginning with the pure polymer, we observe that a plateau stress occurs at long chain length because entangled polymer chains in fibrils cannot become broken. We find that the work needed to dissipate during the failure increases with the increase of [Formula: see text] and [Formula: see text] and the yield point in the stress-strain behavior occurs at a smaller strain for an attractive NPs filled system compared to the pure case, attributed to the more mechanically heterogeneous environment. The thermodynamics of the polyme...
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Motif discovery, which focuses on locating short sequence patterns associated with the regulation of genes in a species, leads to a class of statistical miss- ing data problems. These problems are discussed rst with reference to a... more
Motif discovery, which focuses on locating short sequence patterns associated with the regulation of genes in a species, leads to a class of statistical miss- ing data problems. These problems are discussed rst with reference to a hypothetical model, which serves as a point of departure for more realistic versions of the model. Some general results relating to modeling and
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Angina pectoris is a common symptom imperiling patients' life quality. The aim of this study is to evaluate the efficacy and safety of acupuncture for stable angina pectoris. Clinical randomized-controlled trials (RCTs) comparing the... more
Angina pectoris is a common symptom imperiling patients' life quality. The aim of this study is to evaluate the efficacy and safety of acupuncture for stable angina pectoris. Clinical randomized-controlled trials (RCTs) comparing the efficacy of acupuncture to conventional drugs in patients with stable angina pectoris were searched using the following database of PubMed, Medline, Wanfang and CNKI. Overall odds ratio (ORs) and weighted mean difference (MD) with their 95% confidence intervals (CI) were calculated by using fixed- or random-effect models depending on the heterogeneity of the included trials. Total 8 RCTs, including 640 angina pectoris cases with 372 patients received acupuncture therapy and 268 patients received conventional drugs, were included. Overall, our result showed that acupuncture significantly increased the clinical curative effects in the relief of angina symptoms (OR=2.89, 95% CI=1.87-4.47, P<0.00001) and improved the electrocardiography (OR=1.83, 95%...
Breast cancer-related lymphedema is associated with numerous adverse outcomes. This study investigated the extent clinical factors, lymphedema symptoms, lymphedema-related appearance, and coping strategies predicted quality of life.... more
Breast cancer-related lymphedema is associated with numerous adverse outcomes. This study investigated the extent clinical factors, lymphedema symptoms, lymphedema-related appearance, and coping strategies predicted quality of life. Female patients who underwent microsurgical treatment for lymphedema (n = 54) participated. Lymphedema symptoms were associated with physical and functional well-being, but not emotional and social well-being. Clinical factors and lymphedema-related appearance were not significantly associated with quality of life. Compared to adaptive coping strategies, maladaptive coping strategies (e.g. denial, venting, self-blame) were more strongly associated with quality of life. This suggests psychosocial interventions aimed at modifying maladaptive coping behaviors can potentially improve quality of life for this patient population.
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Spin resonances can cause partial or full depolarization or spin-flip of a polarized beam. We studied 1st-, 2nd- and 3rd-order spin resonances with a 2.1 GeV/c vertically polarized proton beam stored in the COSY Cooler Synchrotron. We... more
Spin resonances can cause partial or full depolarization or spin-flip of a polarized beam. We studied 1st-, 2nd- and 3rd-order spin resonances with a 2.1 GeV/c vertically polarized proton beam stored in the COSY Cooler Synchrotron. We observed almost full spin-flip when crossing the 1st-order G*gamma=8nu<sub>y</sub> vertical-betatron-tune spin resonance and partial depolarization near some 2nd- and 3rd-order resonances. We observed
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Programmed Death-1 (PD-1) and its ligand, PD-L1, are regulators of immune/ inflammatory mechanisms. We explored the potential involvement of PD-1/PD-L1 pathway in the inflammatory response and tissue damage in cardiac injury models.... more
Programmed Death-1 (PD-1) and its ligand, PD-L1, are regulators of immune/ inflammatory mechanisms. We explored the potential involvement of PD-1/PD-L1 pathway in the inflammatory response and tissue damage in cardiac injury models. Ischemic-reperfused and cryoinjured hearts were processed for flow cytometry and immunohistochemical studies for determination of cardiac PD-1 and PD-L1 in the context of assessment of the growth arrest- and DNA damage-inducible protein 153 (GADD153) which regulates both inflammation and cell death. Further, we explored the potential ability of injured cardiac cells to influence proliferation of T lymphocytes. The isolated ischemic-reperfused hearts displayed marked increases in expression of PD-1 and PD-L1 in cardiomyocytes; however, immunofluorescent studies indicate that PD-1 and PD-L1 are not primarily co-expressed on the same cardiomyocytes. Upregulation of PD-1/PD-L1 was associated with a) marked increases in GADD153 and interleukin (IL)-17 but a m...
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The crude processing of electronic waste (e-waste) has led to serious contamination in soils. While microorganisms may play a key role in remediation of the contaminated soils, the ecological effects of combined pollution (heavy metals,... more
The crude processing of electronic waste (e-waste) has led to serious contamination in soils. While microorganisms may play a key role in remediation of the contaminated soils, the ecological effects of combined pollution (heavy metals, polychlorinated biphenyls and polybrominated diphenyl ethers) on the composition and diversity of microbial communities remain unknown. In this study, a suite of e-waste contaminated soils were collected from Guiyu, China, and the indigenous microbial assemblages were profiled by 16S rRNA high-throughput sequencing and clone library analysis. Our data revealed significant differences in microbial taxonomic composition between the contaminated and the reference soils, with Proteobacteria, Acidobacteria, Bacteroidetes and Firmicutes dominating the e-waste-affected communities. Genera previously identified as organic pollutants-degrading bacteria, such as Acinetobacter, Pseudomonas and Alcanivorax, were frequently detected. Canonical correspondence anal...
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Loops in proteins are often involved in biochemical functions. Their irregularity and flexibility make experimental structure determination and computational modeling challenging. Most current loop modeling methods focus on modeling... more
Loops in proteins are often involved in biochemical functions. Their irregularity and flexibility make experimental structure determination and computational modeling challenging. Most current loop modeling methods focus on modeling single loops. In protein structure prediction, multiple loops often need to be modeled simultaneously. As interactions among loops in spatial proximity can be rather complex, sampling the conformations of multiple interacting loops is a challenging task. In this study, we report a new method called multi-loop Distance-guided Sequential chain-Growth Monte Carlo (m-DiSGro) for prediction of the conformations of multiple interacting loops in proteins. Our method achieves an average RMSD of 1:93 Å for lowest energy conformations of 36 pairs of interacting protein loops with the total length ranging from 12 to 24 residues. We further constructed a data set containing proteins with 2, 3, and 4 interacting loops. For the most challenging target proteins with 4 ...
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To construct the expressing vector pcDNA3.0- wildtype-syndecan-1(WT-sdc1) and pcDNA3.0-unshedding-syndecan-1(uS-sdc1), and to explore the expression in vitro. (1)The mouse WT-sdc1 DNA was successfully amplified by PCR and then cloned into... more
To construct the expressing vector pcDNA3.0- wildtype-syndecan-1(WT-sdc1) and pcDNA3.0-unshedding-syndecan-1(uS-sdc1), and to explore the expression in vitro. (1)The mouse WT-sdc1 DNA was successfully amplified by PCR and then cloned into pcDNA3.0. uS-sdc1 was construct by Gene splicing by overlap extension- PCR on the basis of WT-sdc1. The two vectors confirmed by DNA sequencing. (2)there are 4 groups in our research: control group, pcDNA3.0 transfected group, WT-sdc1 transfected group and uS-sdc1 transfected group. each vecter was transfected into IEC-6 cells by Lipofectamine(TM);2000. RT-PCR, Western blot and Dot blot were performed to detect the expression of syndecan-1 before and after stimulation of phorbol 12-myristate 13-acetate (PMA) for 15 min. The vector WT-sdc1 and uS-sdc1 were successfully constructed although an non-sense mutation was in uS-sdc1. Compared to control and pcDNA3.0 transfected groups, WT-sdc1 and uS-sdc1 groups showed a significant increase in the express...
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The safety and efficacy of rivaroxaban in the periprocedural anticoagulation for patients undergoing catheter ablation of atrial fibrillation is not well established. We sought to systematically review this evidence using data from... more
The safety and efficacy of rivaroxaban in the periprocedural anticoagulation for patients undergoing catheter ablation of atrial fibrillation is not well established. We sought to systematically review this evidence using data from multiple studies. A thorough literature search was conducted in MEDLINE, EMABSE, Web of knowledge, clinicaltrials.gov, and the Cochrane library up to November 2014. Studies of at least 100 patients in rivaroxaban and warfarin groups were included. Nine observational studies were identified enrolling a total of 4,334 patients (1,210 treated with rivaroxaban and 3,124 with warfarin). The primary outcomes were thromboembolic events and major bleeding. The fixed-effects model meta-analysis was performed and risk ratios (RRs) were calculated. No significant differences were found between patients treated with rivaroxaban and warfarin with regard to thromboembolic events (0.25% rivaroxaban vs. 0.29% warfarin; RR: 0.61; 95%CI: 0.21-1.76; P=0.36) and major bleedi...
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A novel domino annulation strategy for the construction of benzo[b]thiophenes has been developed. In the presence of Cs2CO3 and Ag2CO3, a wide range of α-alkenoyl-α-alkynyl ketene dithioacetals readily react with cyanoacetates in CH3CN at... more
A novel domino annulation strategy for the construction of benzo[b]thiophenes has been developed. In the presence of Cs2CO3 and Ag2CO3, a wide range of α-alkenoyl-α-alkynyl ketene dithioacetals readily react with cyanoacetates in CH3CN at 110 °C under N2 to afford multisubstituted benzo[b]thiophenes efficiently via tandem thien- and benzannulations. A plausible mechanism is also proposed.
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To investigate the expressions of MAGE-1,-2,-3 and -4 in lung cancer at mRNA level. Using a reverse transcription-polymerase chain reaction assay (RT-PCR), the expressions of MAGE-1,-2,-3 and -4 in 35 lung cancer samples and non-tumorous... more
To investigate the expressions of MAGE-1,-2,-3 and -4 in lung cancer at mRNA level. Using a reverse transcription-polymerase chain reaction assay (RT-PCR), the expressions of MAGE-1,-2,-3 and -4 in 35 lung cancer samples and non-tumorous lung tissues were examined. Of the 35 tumor samples, the MAGE-1,-2,-3 and -4 were expressed at mRNA level in 22.9% (8/35), 62.9% (22/35), 37.1% (13/35) and 77.1% (27/35) respectively. At least one of these genes was expressed in 85.7% (30/35) of the samples. Two or more of these genes were expressed in 71.4% (25/35) of the samples. None of the non-tumorous lung tissue was positive for these genes. No significant difference of the frequency of MAGE gene expression was found between adenocarcinoma and squamous cell carcinoma, between different tumor stages, and between groups with or without lymph node metastasis (P > 0.05). MAGE-1,-2,-3 and -4 are expressed at a high percentage in lung cancer. All of these genes are negative in the adjacent non tu...
Melanoma antigen genes (MAGE) are tumor specific genes. The aim of this study is to explore the feasibility of MAGE genes detection as a diagnostic method for malignant pleural effusion. The expression of MAGE-1, -2, -3 and -4 mRNA was... more
Melanoma antigen genes (MAGE) are tumor specific genes. The aim of this study is to explore the feasibility of MAGE genes detection as a diagnostic method for malignant pleural effusion. The expression of MAGE-1, -2, -3 and -4 mRNA was detected in 18 benign and 22 malignant pleural effusion samples by RT-PCR. No MAGE gene expressed in the 18 cases of benign pleural effusion. Out of the 22 cases of malignant pleural effusion, 8 cases were positive by cytological examination, who all showed positive expression of MAGA genes; in the other 14 patients who were positive by pleural biopsy but negative by cytological examination, 11 cases showed positive expression of MAGE genes in both the pleural effusion and pleura samples, while the other 3 cases showed negative MAGE expression in both the pleural effusion and pleura samples. Detection of MAGE genes in pleural effusion may be an effective method in the differential diagnosis of benign and malignant pleural effusion.
Histone acetylation plays important but incompletely understood roles in gene regulation. A comprehensive understanding of the regulatory role of histone acetylation is difficult because many different histone acetylation patterns exist... more
Histone acetylation plays important but incompletely understood roles in gene regulation. A comprehensive understanding of the regulatory role of histone acetylation is difficult because many different histone acetylation patterns exist and their effects are confounded by other factors, such as the transcription factor binding sequence motif information and nucleosome occupancy. We analyzed recent genomewide histone acetylation data using a few complementary statistical models and tested the validity of a cumulative model in approximating the global regulatory effect of histone acetylation. Confounding effects due to transcription factor binding sequence information were estimated by using two independent motif-based algorithms followed by a variable selection method. We found that the sequence information has a significant role in regulating transcription, and we also found a clear additional histone acetylation effect. Our model fits well with observed genome-wide data. Strikingly...
Research Interests: Algorithms, Genomics, Computational Biology, Gene regulation, Statistical Analysis, and 14 moreBiological Sciences, Saccharomyces cerevisiae, Environmental Sciences, Computer Simulation, Variable Selection, Histone Modification, Combinatorial Complexity, Histones, Acetylation, Statistical Model, Global Gene Expression, Histone Acetylation, Cumulant, and Conditional Independence
Like microarray-based investigations, high-throughput proteomics techniques require machine learning algorithms to identify biomarkers that are informative for biological classification problems. Feature selection and classification... more
Like microarray-based investigations, high-throughput proteomics techniques require machine learning algorithms to identify biomarkers that are informative for biological classification problems. Feature selection and classification algorithms need to be robust to noise and outliers in the data. We developed a recursive support vector machine (R-SVM) algorithm to select important genes/biomarkers for the classification of noisy data. We compared its performance to a similar, state-of-the-art method (SVM recursive feature elimination or SVM-RFE), paying special attention to the ability of recovering the true informative genes/biomarkers and the robustness to outliers in the data. Simulation experiments show that a 5%- approximately 20% improvement over SVM-RFE can be achieved regard to these properties. The SVM-based methods are also compared with a conventional univariate method and their respective strengths and weaknesses are discussed. R-SVM was applied to two sets of SELDI-TOF-M...
Research Interests: Algorithms, Artificial Intelligence, Machine Learning, Mass Spectrometry, Breast Cancer, and 19 moreFeature Selection, Biological Sciences, Humans, Mathematical Sciences, Animals, Cluster Analysis, BMC Bioinformatics, Support vector machine, Rats, Very high throughput, Reproducibility of Results, Simulation experiment, Biological markers, Sensitivity and Specificity, Proteome, Classification Algorithm, Microarray Data, Gene expression profiling, and Differential expression(Feature Selection, Biological Sciences, Humans, Mathematical Sciences, Animals, Cluster Analysis, BMC Bioinformatics, Support vector machine, Rats, Very high throughput, Reproducibility of Results, Simulation experiment, Biological markers, Sensitivity and Specificity, Proteome, Classification Algorithm, Microarray Data, Gene expression profiling, and Differential expression)
(Feature Selection, Biological Sciences, Humans, Mathematical Sciences, Animals, Cluster Analysis, BMC Bioinformatics, Support vector machine, Rats, Very high throughput, Reproducibility of Results, Simulation experiment, Biological markers, Sensitivity and Specificity, Proteome, Classification Algorithm, Microarray Data, Gene expression profiling, and Differential expression)
Hepatitis B virus affects more than two billion people worldwide, 350 million of which have developed chronic hepatitis B (CHB). The genetic factors that confer CHB risk are still largely unknown. We sought to identify genetic variants... more
Hepatitis B virus affects more than two billion people worldwide, 350 million of which have developed chronic hepatitis B (CHB). The genetic factors that confer CHB risk are still largely unknown. We sought to identify genetic variants for CHB susceptibility in the Chinese population. We undertook a genome-wide association study (GWAS) in 2,514 CHB cases and 1,130 normal controls from eastern China. We replicated 33 of the most promising signals and eight previously reported CHB risk loci through a two-stage validation totaling 6,600 CHB cases and 8,127 controls in four independent populations, of which two populations were recruited from eastern China, one from northern China and one from southern China. The joint analyses of 9,114 CHB cases and 9,257 controls revealed significant association of CHB risk with five novel loci. Four loci are located in the human leukocyte antigen (HLA) region at 6p21.3, including two nonsynonymous variants (rs12614 [R32W] in CFB, Pmeta =1.28 × 10(-34...
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Melanoma antigen genes (MAGE genes) are tumor specific genes. In this study the expressions of MAGE-1, -2, -3 and -4 genes in lymph nodes of patients with non-small cell lung cancer (NSCLC) at mRNA level were investigated and the role of... more
Melanoma antigen genes (MAGE genes) are tumor specific genes. In this study the expressions of MAGE-1, -2, -3 and -4 genes in lymph nodes of patients with non-small cell lung cancer (NSCLC) at mRNA level were investigated and the role of MAGE genes was analyzed in the diagnosis of occult micrometastasis in lymph nodes of patients with NSCLC. One hundred and eleven stations of lymph nodes from 53 patients with NSCLC were studied to detect mRNA for MAGE-1, -2, -3 and -4 genes by using reverse transcription-polymerase chain reaction (RT-PCR) assay. The expression rate in samples of lymph nodes using RT-PCR (41.4%, 46/111) was significantly higher than that using routine histopathological examination (27.9%, 31/111). Of the 80 stations of lymph nodes without invasion of the tumor confirmed by routine histopathological examination, at least one of these genes was expressed in (23.8%) (19/80) out of the samples. Of the 31 stations of lymph nodes with invasion of the tumor confirmed by rou...
Haplotype inference from phase-ambiguous multilocus genotype data is an important task for both disease-gene mapping and studies of human evolution. We report a novel haplotype-inference method based on a coalescence-guided hierarchical... more
Haplotype inference from phase-ambiguous multilocus genotype data is an important task for both disease-gene mapping and studies of human evolution. We report a novel haplotype-inference method based on a coalescence-guided hierarchical Bayes model. In this model, a hierarchical structure is imposed on the prior haplotype frequency distributions to capture the similarities among modern-day haplotypes attributable to their common ancestry. As a consequence, the model both allows distinct haplotypes to have different a priori probabilities according to the inferred hierarchical ancestral structure and results in a proper joint posterior distribution for all the parameters of interest. A Markov chain-Monte Carlo scheme is designed to draw from this posterior distribution. By using coalescence-based simulation and empirically generated data sets (Whitehead Institute&amp;amp;amp;amp;#39;s inflammatory bowel disease data sets and HapMap data sets), we demonstrate the merits of the new method in comparison with HAPLOTYPER and PHASE, with or without the presence of recombination hotspots and missing genotypes.
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A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of single-intensity-based sensing mechanism... more
A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility and stability. Within a sensing temperature range of 30 to 55°C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo.
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The inhibition of serum glucocorticoid-regulated kinase-1 (SGK-1) has been found to decrease growth of colon and prostate cancer cells. The purpose of this study is to evaluate the therapeutic effect of SGK-1 inhibition in head and neck... more
The inhibition of serum glucocorticoid-regulated kinase-1 (SGK-1) has been found to decrease growth of colon and prostate cancer cells. The purpose of this study is to evaluate the therapeutic effect of SGK-1 inhibition in head and neck squamous cell carcinoma (SCC). Human head and neck tumors (HTB41/43) were established in athymic mice. Growth rates between mice treated with vehicle (PBS) injection (group 1, n = 5), SGK-1 Inhibitor GSK 650394 (group 2, n = 6), systemic cisplatin (group 3, n = 6), and a combination of SGK-1 Inhibitor and cisplatin (group 4, n = 6) were compared using repeated measures one-way ANOVA with Newman-Keuls Multiple Comparison Test. Tumor cells were subsequently submitted to further analyses. At the end of the experiment mean tumor sizes were 122.33+/-105.86, 76.73+/-36.09, 94.52+/-75.92, and 25.76+/-14.89 mm2 (mean +/- SD) for groups 1 to 4. Groups 2 and 3 showed decreased tumor growth compared to controls (p<0.001). Group 4 displayed even greater growt...
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To evaluate the effect and side effect of T(3) versus T(4) sympathectomy in the surgical management of primary palmar hyperhidrosis through prospective multi-center randomized controlled trial. From Sep 2004 to Feb 2006, 106 consecutive... more
To evaluate the effect and side effect of T(3) versus T(4) sympathectomy in the surgical management of primary palmar hyperhidrosis through prospective multi-center randomized controlled trial. From Sep 2004 to Feb 2006, 106 consecutive patients (49 males and 54 females) with primary palmar hyperhidrosis were enrolled in the study and randomly assigned into T(3) or T(4) level sympathectomy groups. 66 patients received T(3) sympathetic chain transection and 37 patients received T(4) sympathetic chain transection. The two groups were comparable in all other parameters. All procedures were carried out successfully without severe morbidity or mortality. After a mean follow-up period of 8.6 months, both groups remained satisfactory in palmar sweating control. The T(4) group had more moisture hands compared with the T(3) branch (54.1% vs 15.2%, P < 0.01), but this didn't compromise the patients' satisfactory. The patients in T(4) group showed significantly less dry hand problem...
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To investigate the feasibility, safety and prospect application value of video-assisted thoracoscopic sympathectomy utilized in the treatment of craniofacial hyperhidrosis. Eighteen patients with craniofacial hyperhidrosis underwent... more
To investigate the feasibility, safety and prospect application value of video-assisted thoracoscopic sympathectomy utilized in the treatment of craniofacial hyperhidrosis. Eighteen patients with craniofacial hyperhidrosis underwent sympathectomy from January 2003 to December 2004, including 10 cases combined with palmar hyperhidrosis. There were 17 males and 1 females with a mean age of 36.5 years (ranges, 25-42). Sympathetic chain was transected just below the stellate ganglion in pure craniofacial hyperhidrosis. Additional T(3) sympathectomy was performed in those patients combined with palmar hyperhidrosis. There were no surgical complication or surgical mortality cases. Seventeen patients achieved improvement of craniofacial hyperhidrosis without recurrent symptoms, but one had partial remission due to thoracic adhesion and false location of the ganglions. The effective rate was 97% (35/36). No recurrent symptoms occurred after a mean of 8.3 months of follow-up. No relapse occu...
Research Interests: Face, Treatment Outcome, Humans, Hyperhidrosis, Female, and 4 moreMale, Follow-up studies, Head, and Adult
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To clone and identify genes differentially expressed in human lung squamous cell carcinoma (LSCC). A subtracted cDNA library of human LSCC was constructed by suppression subtracted hybridization (SSH) method. After screening, the... more
To clone and identify genes differentially expressed in human lung squamous cell carcinoma (LSCC). A subtracted cDNA library of human LSCC was constructed by suppression subtracted hybridization (SSH) method. After screening, the subtracted library clones representing mRNAs that were truly differentially expressed in LSCC but not in its adjacent non cancerous tissues were selected to identify by RT-PCR and DNA sequencing were performed. Nucleic acid homology searches were performed using the BLAST program. By this technique, 10 differentially expressed gene cDNA fragments of LSSC were obtained. Two were novel and eight were already known genes. SSH is a useful technique with high sensitivity for the detection of differential genes expression in LSCC and an effective method to clone novel genes.
To investigate whether autologous lung cancer tissues derived gp96-peptide complex/dendritic cell vaccine could induce peptide specific cytotoxic T lymphocyte (CTL) response in vitro. A patient's tumor-derived antigens including... more
To investigate whether autologous lung cancer tissues derived gp96-peptide complex/dendritic cell vaccine could induce peptide specific cytotoxic T lymphocyte (CTL) response in vitro. A patient's tumor-derived antigens including gp96-peptide complexes and tumor cell lysate were co cultured with DCs derived from the same patient's bone marrow blood mononuclear cells. The various antigen/DC vaccines were used to stimulate peripheral lymphocytes. Interferon-γ (IFN-γ) level of activated lymphocytes was detected by ELISA method and the Cr51 release test was performed to evaluate the gp96-peptide specific CTL response in three kinds of target cells including the primary cultured tumor cells, PG cells and K562 cells. IFN-γ could be observed from the supernate collected in all antigen groups after the cognate T lymphocytes were stimulated by various vaccines. The concentration of IFN-γ induced by gp96-peptide complexes/DC vaccine was higher than that of other groups. In addition, th...
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The use of UV/ozone surface treatments for achieving low temperature bonds between PMMA and COC microfluidic substrates is evaluated. Low temperature bond strengths, approaching those of native polymer substrates bonded above their glass... more
The use of UV/ozone surface treatments for achieving low temperature bonds between PMMA and COC microfluidic substrates is evaluated. Low temperature bond strengths, approaching those of native polymer substrates bonded above their glass transition temperatures, are demonstrated for both thermoplastics. To evaluate the effects of the UV/O3 surface treatment on the operation of bonded microfluidic devices, the relationship between UV/O3
Research Interests: Engineering, Microfluidics, Electrochemistry, Lab On A Chip, Polymers, and 18 moreSurface Chemistry, Temperature, Bond Strength, CHEMICAL SCIENCES, Glass Transition, Glass Transition Temperature, Surface treatment, Ozone, Oxygen, Polyvinyl alcohol, Surface Properties, Very high throughput, Osmosis, Low Temperature, X Ray Photoelectron Spectroscopy, Electroosmosis, Hydrogen-Ion Concentration, and Polymethyl Methacrylate (PMMA)(Surface Chemistry, Temperature, Bond Strength, CHEMICAL SCIENCES, Glass Transition, Glass Transition Temperature, Surface treatment, Ozone, Oxygen, Polyvinyl alcohol, Surface Properties, Very high throughput, Osmosis, Low Temperature, X Ray Photoelectron Spectroscopy, Electroosmosis, Hydrogen-Ion Concentration, and Polymethyl Methacrylate (PMMA))
(Surface Chemistry, Temperature, Bond Strength, CHEMICAL SCIENCES, Glass Transition, Glass Transition Temperature, Surface treatment, Ozone, Oxygen, Polyvinyl alcohol, Surface Properties, Very high throughput, Osmosis, Low Temperature, X Ray Photoelectron Spectroscopy, Electroosmosis, Hydrogen-Ion Concentration, and Polymethyl Methacrylate (PMMA))
We measured the infrared vibrational response of two prototypical molecule-based magnets, [Ru2(O2CMe)4]3[Cr(CN)6] and Mn[N(CN)2]2. We find that both temperature and magnetic field driven transitions impact spin-lattice interactions in... more
We measured the infrared vibrational response of two prototypical molecule-based magnets, [Ru2(O2CMe)4]3[Cr(CN)6] and Mn[N(CN)2]2. We find that both temperature and magnetic field driven transitions impact spin-lattice interactions in these materials. For instance, through the N&amp;amp;amp;amp;amp;amp;#39;eel transition, Cr--CN stretching and bending modes as well as Ru-O stretching mode in [Ru2(O2CMe)4]3[Cr(CN)6] display sudden frequency shifts and a strong hysteresis that reveal local
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Surface spectral albedo, a factor that strongly affects Earth&amp;amp;amp;#39;s radiation balance, is a boundary condition which needs to be known with high accuracy for aerosol remote sensing, surface aerosol forcing, and radiative... more
Surface spectral albedo, a factor that strongly affects Earth&amp;amp;amp;#39;s radiation balance, is a boundary condition which needs to be known with high accuracy for aerosol remote sensing, surface aerosol forcing, and radiative transfer calculations. We use Solar Spectral Flux Radiometer (SSFR) measurements of upward and downward irradiance to determine the area resolved surface spectral albedo (along the aircraft flight track)
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The permutation test is one of the oldest techniques for making statis- tical inferences. Monte Carlo methods and asymptotic formulas have been used to approximate the associated p-values. When data are truncated, however, the permutation... more
The permutation test is one of the oldest techniques for making statis- tical inferences. Monte Carlo methods and asymptotic formulas have been used to approximate the associated p-values. When data are truncated, however, the permutation null distribution is difficult to handle. We describe here an efficient se- quential importance sampling strategy for generating permutations with restricted positions, which provides accurate
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A novel method to enhance the robustness of the microcavity coupling system (MCS) is presented by encapsulating and solidifying the MCS with a low refractive index (RI) curable UV polymer. The encapsulating process is illustrated in... more
A novel method to enhance the robustness of the microcavity coupling system (MCS) is presented by encapsulating and solidifying the MCS with a low refractive index (RI) curable UV polymer. The encapsulating process is illustrated in detail for a typical microsphere with a radius of R about 240mum. Three differences of the resonant characteristics before and after the package are
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Calcineurin, a Ca2+/calmodulin-dependent protein phosphatase, is the common target for two immunophilin-immunosuppressant complexes, cyclophilin A-cyclosporin A (CyPA-CsA) and FKBP-FK506. How the two structurally distinct... more
Calcineurin, a Ca2+/calmodulin-dependent protein phosphatase, is the common target for two immunophilin-immunosuppressant complexes, cyclophilin A-cyclosporin A (CyPA-CsA) and FKBP-FK506. How the two structurally distinct immunophilin-drug complexes bind the same target has remained unknown. We report the crystal structure of calcineurin (CN) in complex with CyPA-CsA at 2.8-Å resolution. The CyPA-CsA complex binds to a composite surface formed by the catalytic
Research Interests:
Summary: The purpose of our Binding-site Estimation Suite of Tools (BEST) is two-fold: to provide a platform for using and compar- ing different motif-finding programs for transcription factor binding site prediction, and to improve the... more
Summary: The purpose of our Binding-site Estimation Suite of Tools (BEST) is two-fold: to provide a platform for using and compar- ing different motif-finding programs for transcription factor binding site prediction, and to improve the accuracy of these predictions by further optimization. Our software package BEST includes four commonly used motif-finding programs: AlignACE, BioProspector, CONSENSUS and MEME, as well as
Research Interests:
K. GARROWb, DF GEESAMANc, P. GUEYEf, K. GUSTAFSSONk, K. HAFIDIc, JO HANSENc, W. HINTONf, HE JACKSONc, H. JUENGSTs, C. KEPPELf , A. KLEINt, D. KOLTENUKq, Y. LIANGu, JH LIUs, A. LUNGb, D. MACKb, R. MADEYfe, P. MARKOWITZab, CJ ...