Very high throughput

The design of scalable and reliable interconnection net-works for System on Chips (SoCs) introduce new design constraints not present in current multicomputer systems. Although regular topologies are preferred for building NoCs, heterogeneous blocks, fabrication faults and reliabil-ity ...

We compare the concept of congestion avoidance with that of ow control and congestion control. A number of possible alternative for congestion avoidance have been identi ed. From these a few were selected for study. The criteria for selection and goals for these schemes ...

We have developed a novel three-dimensional (3D) cellular microarray platform to enable the rapid and efficient tracking of stem cell fate and quantification of specific stem cell markers. This platform consists of a miniaturized 3D cell culture array on a functionalized glass slide for spatially addressable high-throughput screening. A microarray spotter was used to deposit cells onto a modified glass surface to yield an array consisting of cells encapsulated in alginate gel spots with volumes as low as 60 nL. A method based on an immunofluorescence technique scaled down to function on a cellular microarray was also used to quantify specific cell marker protein levels in situ. Our results revealed that this platform is suitable for studying the expansion of mouse embryonic stem (ES) cells as they retain their pluripotent and undifferentiated state. We also examined neural commitment of mouse ES cells on the microarray and observed the generation of neuroectodermal precursor cells characterized by expression of the neural marker Sox-1, whose levels were also measured in situ using a GFP reporter system. In addition, the high-throughput capacity of the platform was tested using a dual-slide system that allowed rapid screening of the effects of tretinoin and fibroblast growth factor-4 (FGF-4) on the pluripotency of mouse ES cells. This high-throughput platform is a powerful new tool for investigating cellular mechanisms involved in stem cell expansion and differentiation and provides the basis for rapid identification of signals and conditions that can be used to direct cellular responses. Biotechnol. Bioeng. 2010; 106: 106–118. © 2010 Wiley Periodicals, Inc.

Advances in molecular genetics have opened opportunities to enhance strategies for genetic improvement of pigs by directly selecting on genes or chromosomal regions that harbor genes that affect traits of interest. In this paper, we review molecular technologies that have become available, the current state of the use of gene- or marker tests in pig breeding programs, and future prospects.

Over the last decade, storage systems have experienced a 10fold increase between their capacity and bandwidth. This gap is predicted to grow faster with exponentially growing concurrency levels, with future exascales delivering millions of nodes and billions of threads of execution. A critical component of future file systems for high-end computing is metadata management. This extended abstract presents ZHT, a zero-hop distributed hash-table, which has been tuned for the specific requirements of high-end computing. The primary goal of ZHT is excellent availability, fault tolerance, high throughput, and low latencies. 1.

Characterization of psychrotrophic lactic acid bacteria (LAB) and Brochothrix thermosphacta communities is needed to understand the microbial ecology of spoilage of modified atmosphere-packed (MAP) meats. To overcome the limitations of the currently used methods for the characterization of psychrotrophic bacterial communities in meat, we developed a culture-independent, 16S rRNA gene-targeted terminal restriction fragment length polymorphism (T-RFLP) method. An identification library

Background: Risk of cardiovascular disease is assessed, in part, by laboratory measurement of the concentra- tions of several lipoproteins. b-Quantification is a method of lipoprotein measurement that uses ultracen- trifugation to partially separate lipoprotein classes. Al- though b-quantification is used largely in clinical and basic research, methods have not been described to allow the analysis of a large number of

A high-throughput mass spectrometry assay to measure the catalytic activity of UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase, LpxC, is described. This reaction is essential in the biosynthesis of lipopolysaccharide (LPS) of gram-negative bacteria and is an attractive target for the development of new antibacterial agents. The assay uses the RapidFire mass spectrometry platform to measure the native LpxC substrate and the reaction product and thereby generates a ratiometric readout with minimal artifacts due to detection interference. The assay was robust in a high-throughput screen of a library of more than 700,000 compounds arrayed as orthogonal mixtures, with a median Z' factor of 0.74. Selected novel inhibitors from the screening campaign were confirmed as binding to LpxC by biophysical measurements using a thermal stability shift assay. Some inhibitors showed whole-cell antimicrobial activity against a sensitive strain of Escherichia coli with reduced LpxC ...