Annual Report 2019 by Hudson Institute of Medical Research

Annual Report 2019

At a glance

296 staff

152 students

50 research groups

283 research publications

CONTENTS About us

Board Chair’s report

Director’s report

Clinical collaboration

Research highlights Reproductive health

Children’s health

Inflammation

Cancer

Awards

Our students

Our community

Our supporters

Board of directors

Research outputs

Business development and commercialisation

Organisation structure

Financial snapshot

2019 Publications

Graduates of 2019

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About us Hudson Institute is a leading Australian medical research institute recognised internationally for discovery science and translational research into reproductive health and pregnancy, infant and child health, inflammation and cancer. Our institute is home to more than 470 world-class scientists who strive to solve complex problems in human disease. Our close ties with clinicians and industry enable us to translate our discoveries into new preventative approaches, therapies and devices for patients. Working alongside clinicians in Melbourne hospitals for more than 50 years, Hudson Institute scientists pioneered IVF and stem cell discoveries and are now leading developments in cell therapies, paediatric cancer and the human microbiome. Our worldwide scientific and medical collaborations provide a foundation for transformative healthcare programs across the globe.

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Our themes We are leaders in four areas of medical need

Reproductive health and pregnancy Addressing the challenges of infertility and complications during pregnancy, and progressing women’s health.

Infant and child health Protecting vulnerable newborns from complications during birth, in the critical early weeks of life and exploring better treatments for childhood diseases.

Inflammation

Cancer

Exploring how our body responds to infection and tissue damage with inflammation, and using this knowledge to fight infectious diseases, cancer and autoimmune diseases.

Investigating the molecular and cellular mechanisms that lead to the development of cancer and how these insights may be used to better diagnose, detect and treat malignancies.

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Board Chair’s report 2019 was a year of unprecedented growth for Hudson Institute. Alongside significant progress in research discoveries, we continued to cement our position as a national and international leader in reproductive health and pregnancy, infant and child health, inflammation and cancer research. We achieved outstanding funding success from the National Health and Medical Research Council, with a total of $21.6 million in grants awarded to Hudson Institute researchers. Our success rate of 21.4 per cent in the Investigator round alone was well above the national average of 13.2 per cent. This is a testament to the dedication of researchers and support from staff

at the Institute. Congratulations to everyone for their efforts. We also have our philanthropic partners and donors to thank for their invaluable support of our researchers’ work. In particular, the Children’s Cancer Foundation for their commitment to Phase II of the Hudson Monash Paediatric Precision Medicine Program, which will expand patient recruitment over the next three years. Our commercialisation arm provides further unique opportunities for growth and investment in life-saving research. We progressed and established significant industry partnerships across multiple research areas in 2019 and look forward to this continued expansion. To assist our focus on funding research, the Board has overseen the review and improvement of research administration and business systems to maintain industry best practices in

transparency, probity and compliance— keeping operating costs to a minimum. In addition, our Scientific Support Group have utilised digital enhancements to improve and streamline ethics applications, staff induction and training, grants and contract management, archival management and researcher travel management. These efforts on operational excellence underpin the research excellence at the Institute. I thank the Board members for their vision and support, CEO Professor Elizabeth Hartland for her outstanding leadership, and our researchers, clinicians and staff for their commitment to improving health through groundbreaking research.

Dr Bob Edgar AM Chair

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Director’s report Our research is tackling some of the most complex medical challenges to improve human health. Our scientists are at the forefront of discovering new diagnostics and treatments to change the lives of patients everywhere. Financial support for this groundbreaking research in a rapidly changing landscape is vital. I’m pleased to report we achieved significant NHMRC funding success in 2019—with six Investigator grants, nine Ideas grants and one Synergy grant awarded. The new Hudson Institute Strategic Plan, 2019–2023, emphasises the value of providing an inspiring environment for our researchers to succeed. As part of this plan, we established the Hudson Institute Emerging Leaders program to provide

our early career researchers with comprehensive leadership training and mentoring. I’m immensely proud of this program and what it will achieve, not only for our talented young researchers, but for the Institute and future healthcare. The generous support of all our philanthropic partners remains pivotal to our research. We are continuing our program to change the terrible statistics for childhood brain cancer, in partnership with the Children’s Cancer Foundation, who committed an additional $4.9 million for Phase II of the Hudson Monash Paediatric Precision Medicine Program. The Ovarian Cancer Research Foundation raised invaluable funds for our ovarian cancer research programs, and we witnessed the incredible generosity and power of individual fundraising from the Metafit fitness community in support of stomach cancer research.

We continue to drive innovation and ensure our discoveries reach patients, in collaboration with our clinical research partner, Monash Health. I am grateful to them, as well as our industry and philanthropic partners, and I thank our researchers, clinicians and students for their passion and dedication to improving people’s lives and the health of our community.

Professor Elizabeth Hartland Director and CEO

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Clinical collaboration Hudson Institute’s medical research spans discovery, translational research and clinical trials, ensuring laboratory breakthroughs are supported every step of the way to become new drugs, devices, treatments or cures to improve patients’ lives. Our scientists work in close collaboration with Monash Health and Monash University to make this possible. As global experts in their field, Hudson Institute scientists share their specialist knowledge with clinicians and nurses by developing clinical guidelines to improve quality of care and patient outcomes.

Our precinct Delivering cutting edge research to patients

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Reducing stillbirth Health services across Australia will have access to new clinical guidelines to prevent stillbirth. Supported by a postdoctoral fellowship from the Centre for Research Excellence in Stillbirth, Dr Miranda Davies-Tuck will lead a project to roll out, evaluate and expand guidelines she developed in 2017, providing clinicians with new parameters to monitor babies in late pregnancy. The project aims to reduce the disproportionately high rate of stillbirth experienced by South Asian women due to differences in their placenta. The guidelines have already been implemented at Victoria’s largest maternity service, Monash Health, halving the stillbirth rate at term for South Asian women to be in line with other women. Dr Miranda Davies-Tuck

Helping babies born with DSD Differences in sex development (DSD) can sometimes make it unclear whether a newborn is a boy or girl. Some cases are mild, but others can be life-threatening. There are potentially hundreds of different causes so diagnosis is difficult and can be lengthy, placing additional pressures on stressed families. Professor Vincent Harley, Dr Nayla Leon and Dr Alejandra Reyes have written a clinical guide, published in The Lancet Diabetes & Endocrinology, to assist specialists who encounter newborn babies with ambiguous genitalia. By enabling faster diagnosis, the guide will be used worldwide to improve decision-making and future quality of life for many children and their families.

L-R: Professor Vincent Harley, Dr Nayla Leon, Dr Alejandra Reyes

MONASH HEALTH

SCHOOL OF CLINICAL SCIENCES, MONASH UNIVERSITY

MONASH CHILDREN’S HOSPITAL

TRANSLATIONAL RESEARCH FACILITY

HUDSON INSTITUTE OF MEDICAL RESEARCH

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REPRODUCTIVE HEALTH

Finding the cause COLLABORATORS University of Queensland and Monash IVF

FUNDERS US Department of Defense

About 176 million women worldwide have endometriosis. Professor Caroline Gargett has long known the debilitating impacts of this disease on women—and has dedicated a significant portion of her career to investigating its cause.

AU$2 million NHMRC Investigator grant to further investigate the cause of endometriosis. Findings from the project will provide information to help the development of new endometriosis drugs. “This is great news for women who have suffered in silence for so long, waiting seven to 10 years for a diagnosis,” Prof Gargett said.

She has personally known women with the condition and seen first-hand how the disease interferes with all aspects of a woman’s life.

WHAT IS ENDOMETRIOSIS?

In Australia and internationally, there has been a groundswell of endometriosis awareness that has led to more funding for research.

Endometriosis occurs when cells from the endometrium grow in other areas of the body. These cells may stick to organs in the pelvic cavity, including the ovaries, fallopian tubes, peritoneal lining, bowel or bladder, forming painful lesions.

In 2019, Prof Gargett received a three-year, US$2.07 million (AU$3.05 million) grant from the US Department of Defense (DoD) and a

Endometriosis can cause infertility and is estimated to cost Australia around $9.5 billion annually in lost productivity and direct healthcare costs.

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Professor Caroline Gargett

of endometriosis NEXT STEPS

AN ANSWER FOR BRIDGET

Prof Gargett’s team brings together molecular geneticists with expertise in endometriosis genetics at the University of Queensland, with Hudson Institute’s endometrial stem cell scientists, to provide new insights into the cause of endometriosis at a molecular and cellular level. The goal is to develop non-invasive diagnostic tests and new treatments.

Bridget Hustwaite suffered six years of intense abdominal pain before she was diagnosed with stage four endometriosis in August 2018.

“Endometriosis is a complex disease and until basic research is undertaken to understand its complexities, there will not be a cure,” Prof Gargett said.

When Bridget was finally diagnosed, endometriosis was found across her bladder, rectum, bowel and pelvic side walls. Despite this, she felt relieved she finally had a diagnosis.

Since early adolescence, the Endometriosis Australia Ambassador and triple j broadcaster was plagued by heavy bleeding, nausea, fatigue and cramping associated with her period.

Bridget Hustwaite

“We need faster diagnosis, better treatments and more education and awareness about endometriosis. The fact this research is being funded is a step in the right direction,” she said.

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CHILDREN’S HEALTH

Tackling fetal growth restriction COLLABORATORS Department of Obstetrics and Gynaecology, Monash University; Monash Health

FUNDERS Cerebral Palsy Alliance Australia, Equity Trustees, L.E.W. Carty Charitable Fund, NHMRC

Fetal growth restriction (FGR) is a condition where an unborn baby does not grow well, making them susceptible to lung, cardiovascular system and brain damage. Associate Professor Suzie Miller’s close collaboration with Monash Health clinicians, who regularly see babies affected by FGR, underpinned the importance of better understanding and treating the condition.

“If we want a treatment to improve brain outcomes in FGR infants, then we need to be treating the mother during pregnancy, rather than trying to repair the brain injury after the baby is born. “If we can treat with melatonin in utero as we are doing in our Monash Health clinical trial, then we can prevent progression of that brain injury. The heart of this approach is treating and preventing long-term neurodevelopmental conditions, like cerebral palsy, in the womb.”

WHAT IS FGR? FGR is a condition where an unborn baby experiences prolonged hypoxia, a lack of oxygen usually caused by problems with the placenta. The baby stops growing and is born very small.

“The placenta doesn’t work optimally in a reasonably high proportion of expectant mums— between six to eight per cent of pregnancies in Australia,” A/Prof Miller said. In 2019 A/Prof Miller and her team published new findings in the journal Frontiers in Physiology, showing the impact of FGR on babies’ brains. A clinical trial is also underway to test a potential new treatment with a compound, melatonin. “We found that brain injury develops in utero and gets worse as the pregnancy progresses,” A/Prof Miller said. “The longer the growth-restricted baby stayed in utero, exposed to prolonged hypoxia, the worse the brain looks.

Associate Professor Suzie Miller

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HOW WE ARE TACKLING FGR • Imaging: Another study led by A/Prof Suzie Miller and Dr Atul Malhotra used advanced imaging techniques to analyse magnetic resonance imaging (MRI) scans to detect subtle brain injury associated with FGR. Dr Atul Malhotra is a consultant neonatologist at Monash Children’s Hospital and a research fellow at Hudson Institute. The study also involved physicists at Monash Biomedical Imaging. The research is ‘immediately transferable’ to the clinic.

Image, Emily King Photography

WHY TACKLE FGR? • More than 20,000 babies are born with FGR in Australia each year • Up to 20 per cent of babies born globally are affected each year • Can cause brain injury, cognitive, learning and behavioural problems, cerebral palsy and autism

• Melatonin: Melatonin, a powerful antioxidant that can reduce oxidative stress, will be given daily to pregnant women in a clinical trial to determine whether it can protect at-risk babies’ developing brains. The trial involves Monash Health and hospitals across Australia and New Zealand, and is led by Dr Kirsten Palmer and Professor Euan Wallace from Monash University and Hudson Institute.

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INFLAMMATION

Unlocking the microbiome A game-changer for disease

COLLABORATORS Wellcome Sanger Institute, EMBL’s European Bioinformatics Institute, London School of Hygiene and Tropical Medicine, Monash University, University of Birmingham

FUNDERS NHMRC, Wellcome Trust, BBSRC, European Molecular Biology Laboratory, UK Medical Research Council

Trillions of microbes in the gut form a vast ecosystem known as the microbiome. Understanding the complex role these bacteria play in disease has the potential to change the paradigm of human health. Dr Sam Forster and his team, together with collaborators from the Wellcome Sanger Institute (UK), are shedding more light on the mysterious microbiome, using methods ranging from growing bacteria, to DNA sequencing and genomic analysis. The results of their research hold significant implications for how the microbiome is studied and for improving treatment of conditions caused by imbalances in bacteria, including infections, inflammatory bowel disease, irritable bowel syndrome, and allergies—diseases of the immune system. Dr Forster undertook a groundbreaking study that isolated more than 100 never-before-seen species from healthy people’s intestines, by growing

Dr Sam Forster

and DNA sequencing bacterial strains. Further research in collaboration with the European Bioinformatics Institute created a ‘blueprint’ of the human gut by identifying almost 2000 more bacteria using computational methods to reconstruct bacterial genomes. Dr Forster said these combined resources would help scientists worldwide investigate how our microbiome keeps us healthy and its role in disease, by enabling researchers to detect which bacteria are present in the human gut more quickly and accurately than ever before. “Our study has led to the creation of the largest public database of genome-sequenced, intestinal bacteria, including sequences of more than 100 novel species. It is already fundamentally changing the way researchers study the microbiome,” Dr Forster said. To understand more about the development of the microbiome, in a separate study, the researchers looked to newborn babies and their type of delivery. Differences were found in the gut bacteria of babies born vaginally—who had more bacteria from their mother—and infants delivered by caesarean section, who had more hospitalacquired bacteria. While the differences evened out by the time babies turned one, the impacts on long-term health are not yet known. Dr Forster and his team at Hudson Institute are now turning their attention to developing a new generation of microbiome-based medicine. “The microbiome field is still an emerging area. We have invested a lot of time developing methods to study it—now we are trying to understand the interactions between the bacteria and the immune system so we can develop better treatments for a diverse range of conditions.”

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2019 RESEARCH HIGHLIGHTS • The discovery of more than 100 new gut bacteria could lead to new diagnostics and treatments for gastrointestinal disorders, infections and immune conditions (Nature Biotechnology, 2019).

• The largest-ever genomic study of the diarrhoea bacterium Clostridium difficile, found it is evolving to thrive on sugar-rich diets, evade common hospital disinfectants and spread easily (Nature Genetics, 2019).

• Almost 2000 species of bacteria are found living in the gut, using computational methods (Nature, 2019).

• Our microbiome experts Dr Edward Giles, Gemma D’Adamo and Dr Sam Forster call for standardised regulation of faecal microbiota transplant (FMT) treatments, to ensure safer microbiota-based medicines (Nature Reviews Microbiology, 2019).

• Babies born via caesarean section have more hospital-acquired bacteria and are more likely to have antimicrobial resistance, while the microbiome of babies born vaginally is mostly from their mother (Nature, 2019).

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CANCER

Clues to stomach cancer discovered FUNDER NHMRC

Hudson Institute researchers have uncovered a case of mistaken identity in the molecular drivers behind stomach cancer, which could lead to improved treatments for a range of cancers.

tailoring of treatments to the genetic makeup of each affected individual. Prof Jenkins said the ability to sequence the entire genetic makeup—the genome—of tumour tissue was a major medical advancement, contributing to a better understanding of stomach cancer and more targeted treatment for patients.

The study, led by Professor Brendan Jenkins and PhD student Jesse Balic, found stomach cancer is driven by a different process from that previously believed. “It’s a welcome finding. Stomach cancer is a disease that often presents in the later stages,” said Prof Jenkins. “Sadly, most patients are diagnosed with advanced disease, where there has been an absence of early symptoms.” Published in Cancer Research, the study investigated how modifications to certain proteins in our bodies have the potential to trigger stomach and other cancers. Modification of a different amino acid to the one originally believed to be important for activating the cancer signalling protein, STAT3, switched on the expression of many other genes involved in cancer cell growth and proliferation. The STAT3 protein is linked to at least half of cancers. Prof Jenkins said the results could lead to the development of new drugs to block the newly discovered cancer pathway. He said current strategies to block STAT3 had been largely ineffective. Chemotherapy would still be the standard of care, but targeting STAT3 with new drugs could allow

Professor Brendan Jenkins, PhD student Jesse Balic

KEY FINDINGS • Modification of an amino acid (serine) in a protein, STAT3, controls expression of other genes that promote stomach cancer • Modification of an amino acid (tyrosine) in STAT3 was previously believed to be a main driver of cancer • Drugs (inhibitors) need to be developed to block serine phosphorylation, rather than tyrosine phosphorylation

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Daniel Wilson (centre) with two of the more than 2000 Metafit coaches Australia-wide, Lyndsay Buchanan (left) and Clarence Ho (right)

IN MEMORY OF DANIEL WILSON Daniel Wilson was a super-fit 37-year-old when he was diagnosed with stomach cancer in March 2019. The much-loved fitness instructor introduced Metafit, a popular High Intensity Interval Training (HIIT) program to Australia. That someone so healthy and active could be struck down by this disease came as a huge shock to his friends, family and the Metafit community. They were devastated when he died just two months later. Daniel’s loved ones started a fundraising campaign to support a PhD scholarship in his memory at Hudson Institute. More than $37,000 has been raised to support a PhD student to progress stomach cancer research. The inaugural recipient of the Daniel Wilson PhD scholarship is Alice West, who will work with Prof Jenkins’ lab to advance knowledge on the role of the innate immune system in stomach cancer.

Daniel Wilson

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AWARDS

Associate Professor Rebecca Lim

Saving lives with cell therapies In 2019, Associate Professor Rebecca Lim was ranked the highest applicant in the National Health and Medical Research Council (NHMRC) Career Development Fellowship (Industry) Scheme and named one of Australia’s most influential AsianAustralians under 40. Tell us about your research… My cell therapy research focuses on understanding how cells from the amniotic sac can help reverse lifethreatening conditions. I work with industry professionals and clinicians to translate the research into safe and effective technologies and treatments. What are amniotic epithelial cells? Amniotic epithelial cells (amnion cells) are from the amniotic sac that surrounds a baby during pregnancy. They have stem cell-like properties and can grow into many cell types. Most importantly, they have potent effects on inflammation and tissue damage.

What is exciting about this research? Amnion cells offer a simple treatment— they don’t need to be matched to the patient’s blood or tissue type and can be delivered intravenously in about an hour. Who is benefiting? We think amniotic stem cells can help extremely premature babies with lung disease (bronchopulmonary dysplasia, BPD—which can cause cerebral palsy), as well as adults with acute stroke or chronic liver disease. We held a world-first safety trial for cell-based therapies for these diseases. The trials were to determine safety, not efficacy; however, we have begun to see some encouraging results. Tell us more about the preterm babies… A safety trial at the Monash Children’s Hospital involved six preterm babies with BPD who were given a single, low dose of amnion cells. The cells work by attaching themselves to the damaged lungs and kick-starting the organs’ own

repair process. The results showed this cell therapy can be safely used in babies with lung disease. Our current trial involves 24 extremely premature babies from Monash Children’s Hospital and the Royal Women’s Hospital, who are at high risk of BPD. The study’s goal is to determine the optimum cell dosage and frequency for these babies. What is the long-term goal? We want to develop a treatment for premature babies, accessible in hospitals around the world, to increase survival rates and stop long-term complications for these vulnerable infants. COLLABORATORS Monash Children’s Hospital, Monash University, Royal Women’s Hospital

FUNDERS Fielding Foundation, Hugh Rogers Foundation, NHMRC, Jack Brockhoff Foundation

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L–R: Dr Courtney McDonald, Dr Miranda Davies-Tuck , Dr Robert Galinsky, Dr Stacey Ellery, Dr Cristina Giogha, Dr Jun Yang

Supporting our emerging leaders Hudson Institute’s Emerging Leaders program was established to propel our early career researchers to independent careers. Six high-achieving scientists were chosen to participate in the inaugural program, which will provide comprehensive leadership training and mentoring.

AWARDEES Dr Miranda Davies-Tuck Dr Davies-Tuck is a perinatal epidemiologist. Her integrated research program spans laboratory-based discovery research, clinical trials, implementation science, and health policy to identify targets and develop interventions to prevent the devastating tragedy of stillbirth. Dr Stacey Ellery Dr Ellery investigates utero-placental and fetal energy homeostasis during pregnancy. She is particularly interested in how adjustments to maternal diet can improve outcomes in pregnancies complicated by preeclampsia, fetal growth retardation, preterm birth and intrapartum asphyxia. Dr Robert Galinsky Dr Galinsky’s research focuses on early detection and treatment of brain injury that arises during pregnancy or around the time of birth, to reduce the incidence and severity of lifelong disability.

Dr Cristina Giogha Dr Giogha studies several gastroenteritis-causing bacteria to understand how they cause disease and to develop more effective treatments. Dr Courtney McDonald Dr McDonald investigates umbilical cord blood cells to treat perinatal brain injury. Her group hopes to identify key cells that can be used to protect the developing brain. Dr Jun Yang Working across Hudson Institute, Monash Health and Monash University, endocrinologist Dr Yang is investigating ways to optimise the diagnosis of a potentially curable form of high blood pressure, primary aldosteronism, so as to transform clinical practice for better cardiovascular outcomes.

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OUR STUDENTS

Making an impact 152

Dr Nadia Bellofiore has received an award recognising worldleading research undertaken by a student for her PhD thesis.

STUDENTS 113 PHD 4 MASTERS 35 HONOURS

28 STUDENTS WITH MEDICAL TRAINING

Dr Nadia Bellofiore

43 STUDENT FIRST AUTHOR PUBLICATIONS

In 2015, an observation by Dr Nadia Bellofiore led to the groundbreaking discovery that a desert rodent menstruates. The discovery generated significant interest from scientists worldwide, as it could potentially provide a preclinical model to study, and therefore better understand, human menstrual disorders. Previously, scientists largely believed that no rodent species menstruated. Dr Bellofiore’s PhD thesis received the 2018 Vice Chancellor’s Commendation for Thesis Excellence from Monash University. The award recognises world-leading research undertaken by a student. The team went on to discover that this particular rodent, the spiny mouse, also experiences premenstrual-type symptoms similar to humans. “We have shown how menstrual studies in a small and readily

accessible preclinical model could be potentially translated to humans,” Dr Bellofiore said. “This could significantly advance research in women’s reproductive health and help us to understand menstrual disorders such as endometriosis and abnormal uterine bleeding.” Dr Bellofiore paid tribute to her supervisors, Dr Jemma Evans, Dr Fiona Cousins and Associate Professor Peter Temple-Smith (Monash University), adding that they continue to be inspirational mentors to her and other students. COLLABORATORS Monash University

FUNDERS Fielding Foundation, Monash University, Victorian Government

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OUR COMMUNITY

Sharing our knowledge We share our research discoveries to educate and inspire the wider community about the possibilities medical science offers to prevent, treat and cure disease as well as improve health.

YOUNG WOMEN IN SCIENCE Gender equity is an important focus of Hudson Institute. A group of young high school women spent two weeks at the Institute being mentored by female scientists as part of the inaugural Young Women in Science program, which aims to encourage aspiring young scientists to consider a career in the field.

Professor Elizabeth Hartland with Young Women in Science candidates

Public forums

A BRIGHTER FUTURE FOR CEREBRAL PALSY Every 15 hours, an infant in Australia is born with a brain injury that underlies cerebral palsy. Early diagnosis and intervention is critical for maximising an individual’s potential. In October, our researchers shared how their work is revealing more about the causes of cerebral palsy and leading to the development of new therapies. Attendees also heard from parents of children with cerebral palsy, carers and physical therapists.

THE SCIENCE OF REPRODUCTION: EARLY PREGNANCY AND A HEALTHY START TO LIFE Leading reproductive health scientists and clinicians shared their latest fertility and pregnancy research at this September forum. Topics included early pregnancy, IVF, nutrition, and the critical importance of the placenta and the womb environment to developing babies. The forum also featured the artwork, The Biggest Placenta in the World, by Bec Vandyk.

MALE FERTILITY— A LOOK UNDER THE COVER Infertility affects one in five Australian men and women. Reproductive scientists and clinicians outlined the causes, diagnosis and treatments for male infertility at this public forum in November. They also provided the most up-to-date information on improving fertility and how a man’s health at conception affects his child’s development.

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OUR SUPPORTERS

Targeting childhood cancer Sarah Russell knew something was gravely wrong with one of her twins, five-year-old Jaxon, when he became seriously ill with vomiting, nausea and unusual bruising, while his brother, Hunter, remained well.

Jaxon and Sarah

“Normally they get sick together,” Sarah said. “Jaxon was very tired, not eating and had a really nasty cough. An x-ray showed he had a mass on his chest, which is one of the symptoms of leukaemia.” Jaxon was diagnosed in October 2019 with Acute Lymphoblastic Leukaemia (ALL). However, molecular analysis showed he had a rare mutation in his cancer that, although indicating he had a serious form of ALL, would respond best to a specific chemotherapy drug. He began an intense, nine-month regime of this drug and was considered cancer-free after one month. Despite this, Jaxon will need to continue treatment for two-and-a-half years to ensure the cancer doesn’t return. It’s patients like Jaxon who could potentially benefit from the targeted molecular approach of the Hudson Monash Paediatric Precision Medicine Program. A biobank of childhood cancer samples has been established and a range of precision medicine approaches is used to trial and develop targeted treatments for individual patients, giving them the best chance of survival and quality of life. “Many years ago, Jaxon might not have had the chance that he has today to overcome this cancer,” said Sarah, who is looking forward to the day her son can run around again and start school. Jaxon at Monash Children’s Hospital

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The Hudson Monash Paediatric Precision Medicine Program hopes to significantly improve treatment, survival rates and quality of life for childhood cancer patients. With generous funding from the Children’s Cancer Foundation since 2017, Hudson Institute scientists established a living biobank of paediatric cancers to trial and develop targeted treatments. The initiative includes a functional genomic program and uses individual patients’ cancer cells

to identify new targeted therapies. The program is expanding patient recruitment to about 150 children over the next three years, while enabling cutting-edge molecular analyses for each child’s cancer, utilising a paediatric cancer molecular tumour board to translate findings into clinical application. The Children’s Cancer Foundation committed an additional $4.968 million in 2018 for Phase II of the program. childrenscancerfoundation.com.au

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Uniting to fight ovarian cancer IVANHOE GIRLS’ GRAMMAR SCHOOL FUNDRAISER Dr Amy Wilson was the inaugural recipient of the Ivanhoe Girls’ Early Career Ovarian Cancer Research Travel Award, created to foster the career of promising early career ovarian cancer researchers.

Dr Amy Wilson and Ivanhoe Girl’s Grammar students

Dr Maree Bilandzic

L-R: Laura Moffitt, Dr Amy Wilson, Dr Maree Bilandzic

Student Nazanin Karimnia

OCRF SUPPORTS HUDSON INSTITUTE RESEARCH

RESEARCHERS GET BEHIND OCRF

ADDITIONAL OVARIAN CANCER SUPPORT

Dr Maree Bilandzic will progress research into deadly ovarian cancer ‘leader cells’ thanks to funding from the Ovarian Cancer Research Foundation (OCRF). “These chemotherapy-resistant cells thrive in response to treatment,” said Dr Bilandzic, whose study on leader cell research was published in the journal Cancers. The $800,000 OCRF grant will allow Dr Bilandzic to screen drugs that are already on the pharmacy shelf to unlock their hidden potential to fight ovarian cancer, which has a five-year survival rate of less than 30 per cent.

Dr Bilandzic joined some of Australia’s most influential women as an OCRF Ambassador for the Witchery White Shirt Campaign, which raised $1.4 million for ovarian cancer research. In October, Dr Bilandzic and Dr Wilson swapped their lab coats for frocks as part of the OCRF’s Frocktober campaign, which invites the community to frock up, raise funds and ‘frock cancer’. Frocktober raised more than $500,000 for ovarian cancer research.

• Dr Bilandzic was the recipient of a $70,000 Perpetual IMPACT Philanthropy grant focused on a new cell marker that could be used to measure treatment response. • PhD student Nazanin Karimnia received renewed support from the Dr Sue Fowler Scholarship in Ovarian Cancer to continue her work investigating how targeting a unique marker disrupts cells that lead tumour invasion.

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Thank you to our supporters We are grateful for the gifts received from individuals, trusts, foundations and organisations during the year.

Ferring Research Institute

The Ian Potter Foundation

Harold & Cora Brennen Benevolent Trust

The Kenneth Rainin Foundation

Harold Mitchell Foundation

We also acknowledge the support of the Victorian State Government through the Operational Infrastructure Support Program and the Australian Government through its funding bodies including the NHMRC. These valuable contributions assist our scientists to undertake and progress life-changing research.

Inner Wheel Australia

The Royal Australasian College of Physicians Foundation

FUNDING BODIES

Melbourne IVF Pty Ltd

Australasian Sleep Association Australian Communities Foundation Australian Lions Childhood Cancer Research Foundation Australian Research Council Cancer Australia Cancer Council Victoria Carrie’s Beanies 4 Brain Cancer Foundation Cell Care Australia Cerebral Palsy Alliance Children’s Cancer Foundation Cure Brain Cancer Foundation Department of Defense (USA) Department of Economic Development, Jobs, Transport and Resources— Victorian Government Department of Health—Australian Government

Isabella and Marcus Paediatric Brainstem Tumour Fund

The Kids’ Cancer Project

The Scottish Cot Death Trust

La Trobe University

Universities of Australia: AustraliaGermany Joint Research Co-operation Scheme

LEW Carty Charitable Fund

Victorian Cancer Agency

Mayne Pharma

Youanmi Foundation

Medical Research Future Fund— Australian Government

MAJOR DONORS

Jerome Lejeune Foundation

Monash IVF Group Monash University My Room National Health and Medical Research Council

Professor Michael Adamson Professor Warwick Anderson AM Professor Henry Burger AO Professor Arthur Clark Mrs Joan Donaldson Mrs Patricia Donges

Ovarian Cancer Research Foundation

Dr Robert Edgar AM

Peninsula And Southeast Oncology

Mrs Andrea Evans

Perinatal Society of Australia & New Zealand

Estate of the late Judith Anne Fisher

Perpetual Trustees

Professor John Funder AC

Piers K Fowler Trust

Professor Caroline Gargett

Rebecca L Cooper Medical Research Foundation

Mr Richard Harbig

Red Nose

Ivanhoe Girls’ Grammar School

Robert Connor Dawes Foundation

Mrs Kathleen Johnston AM

Science and Industry Endowment Fund

Mrs Christina Kirkland

Scinogy Pty Ltd Snowdome Foundation

Deutsche Forschungsgemeinschaft (German Research Foundation)

The Andrea Joy Logan Trust Fund

Equity Trustees

The Endocrine Society of Australia

European Society of Human Reproduction & Embryology

The Financial Markets Foundation for Children

Evans Family Foundation

The Heart Foundation

The CASS Foundation

Mr John Fowler

Professor Mark Hedger

Professor Kate Loveland Mr Lance Matheson Metafit Ms Julie Muir Mrs Jill Ross-Perrier The Phoebe and Jacob Jones Trust The Sorry Boys

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Board of directors The directors of Hudson Institute of Medical Research Board, 31 December 2019

DR ROBERT (BOB) EDGAR AM BOARD CHAIR BEcon (Hons), PhD, FAICD

PROFESSOR CHRISTINA MITCHELL AO MBBS, PhD, FRACP

PROFESSOR WARWICK ANDERSON AM BS (Hons) UNE, PhD, DUniv, FAHA (Int), FRCPA (Hon), FAAHMS, DH

MR NIGEL GARRARD BEcomm, AICD, CA FAMI Chair, Hudson Foundation

MR ANDREW LEYDEN BComm Chair, Investment Committee; Member, Hudson Foundation

ANNUAL REPORT 2019 | 25

Board committees Finance and Audit Committee Mr Chris Dodd–Chair, Mr David Hanna, Mr Rob Merriel (Secretary), Professor Elizabeth Hartland, Mr Alan Lahiff. Investment Committee Mr Andrew Leyden–Chair, Mr Nigel Garrard, Mr Rob Merriel, Mr Alan Lahiff (Secretary). Intellectual Property and Commercialisation Committee Ms Zita Peach–Chair, Dr Andrew Gearing, Dr Michael Pannacio, Dr Tony Eglezos, Mr Rob Merriel (Secretary), Professor Elizabeth Hartland, Associate Professor Claudia Nold, Ms Carmela Monger and Dr Kate Mackin.

MR ROB MERRIEL COMPANY SECRETARY BA, Grad Dip (Psych), Grad Dip (Accounting), CPA

MR ANDREW STRIPP BBSc (Hons), MSc Board Observer

MS ZITA PEACH

PROFESSOR KIM CORNISH

BSc, FAICD, FAMI Chair, Intellectual Property and Commercialisation Committee

BS (Hons), PhD

MR CHRIS DODD MR DAVID HANNA BEc, BA, GAICD

MBA, FCAANZ Chair, Finance and Audit Committee

26 | HUDSON INSTITUTE OF MEDICAL RESEARCH

Research outputs Grant funding received in 2019

Children’s Cancer Foundation Cancer Australia Carrie’s Beanies for Brain Cancer Ovarian Cancer Research Foundation Cerebral Palsy Alliance Australian Research Council (ARC)

International Grants

Other Trusts and Foundations

Other Australian Government National Health & Medical Research Council (NHMRC)

FUNDING BODIES ($) National Health and Medical Research Council (NHMRC)

PUBLICATIONS 14,247,695

Cancer Australia

1,356,872

Children's Cancer Foundation

1,281,623

In 2019, Hudson Institute’s researchers published extensively in international peer-reviewed journals.

Carrie’s Beanies for Brain Cancer

799,624

Publication type

Ovarian Cancer Research Foundation

586,609

Original research articles

Cerebral Palsy Alliance

552,624

Australian Research Council (ARC)

398,018

Other Australian Government

392,571

Other Trusts and Foundations The Financial Markets for Children Victorian Cancer Agency Eva and Les Erdi Charitable Foundation Science and Industry Endowment Fund Perpetual Trustees IMPACT Cure Brain Cancer The Kids’ Cancer Project Cancer Council Victoria Rebecca L Cooper Foundation Other trusts and foundations TOTAL

286,369 225,000 200,000 193,180 152,099 128,568 104,588 100,000 100,000 1,927,728 3,417,532

International Grants Department of Defense (USA) Ferring Research Institute Other international grants TOTAL

1,233,507 256,263 104,155 1,593,925

TOTAL

24,627,093

2017

2018

2019

273

206

203

Reviews

Editorials and commentaries

Books and book chapters

ANNUAL REPORT 2019 | 27

BUSINESS DEVELOPMENT AND COMMERCIALISATION

From research discovery to patient care The Business Development team ensures the Institute’s research discoveries reach patients. Working with academic, industry and government partners, research is protected, commercialised and pathways are built for its future use.

Partnerships are also forged with global pharmaceutical, clinical and venture capital organisations to progress research from early stages to clinical trials. Diagnostics and treatments for cancer, inflammatory disease and reproductive health are among the new discoveries now in the developmental pipeline.

Significant venture capital funding was invested in a number of projects. The team supported development and protection of intellectual property arising from the Institute’s research, with new patent filings and patents granted.

Dr Tracey Edgell

Associate Professor Rebecca Lim

Dr Jason Cain, Associate Professor Ron Firestein

TIMING TRANSFER FOR SUCCESS

GENERATING NEW TREATMENTS

To improve IVF success rates, Dr Tracey Edgell developed a test to indicate the optimal time for embryo transfer. Following a clinical validation trial, the next step is to make the test available to IVF patients.

Associate Professor Rebecca Lim is collaborating with academic and healthcare partners to develop stem cell therapies to treat a number of conditions, including lung disease in babies (bronchopulmonary dysplasia (BPD)) and adult stroke (see page 16 for more details). Following a worldfirst safety trial of stem cells in acute stroke patients, conducted with La Trobe University and Monash Health, the Institute is supporting A/Prof Lim to take these new regenerative medicine treatments to patients.

BUILDING PRECISION MEDICINE NETWORKS Associate Professor Ron Firestein and Dr Jason Cain are progressing precision medicine for childhood cancer patients. By building expertise and sharing resources in partnerships with other Australian and international research institutes, their findings can impact the greatest number of patients worldwide.

28 | HUDSON INSTITUTE OF MEDICAL RESEARCH

Organisation structure BOARD OF DIRECTORS COMMITTEES

BOARD SUBCOMMITTEES

Career Development Committee Culture and Engagement Committee Early Career Researcher Committee Education and Training Committee Equity and Diversity Committee Ethics Committee Hudson Institute Student Society OHSE Committee Research Committee Seminar Committee

Finance and Audit Committee Investment Committee Intellectual Property and Commercialisation Committee

EXECUTIVE

Associate Director Professor Paul Hertzog

Chief Financial Officer Chief Commercialisation Officer Company Secretary Mr Rob Merriel

Director Professor Elizabeth Hartland

CENTRE HEADS

Centre for Cancer Research Associate Professor Ron Firestein

Centre for Endocrinology and Metabolism Professor Peter Fuller

Senior Operations Manager Dr Joseph Pereira

Centre for Innate Immunity and Infectious Diseases Professor Paul Hertzog

Head of Philanthropy and Fundraising Ms Kay Blandthorn

Centre for Reproductive Health Professor Kate Loveland

Executive Officer Ms Ann Scott

The Ritchie Centre Professor Stuart Hooper

SCIENTIFIC SUPPORT GROUP

TECHNOLOGY PLATFORMS AND CAPABILITIES

MHTP Platform Strategic Initiatives Manager Ms Vivien Vasic

PLATFORMS Cell Therapies Flow Cytometry Functional Genomics Histology Mass Spectrometry Medical Genomics Micro Imaging

ANNUAL REPORT 2019 | 29

Financial snapshot Revenue 1% 3% 7%

Revenue

2019 ($)

2018 ($)

2017 ($)

28,408,235

27,875,344

22,899,072

3,172,168

3,336,283

3,089,225

Philanthropic grants

8,168,395

6,957,795

7,476,138

Commercial research

3,984,044

3,547,176

3,649,352

Infrastructure Monash University

3,616,999

3,198,626

2,474,291

Other income

1,485,615

2,122,605

2,493,126

586,932

625,873

672,316

49,432,388

47,663,699

42,753,519

Australian Government

Victorian Government

57% 17%

Investment income Total 6%

Expenditure

82%

Expenditure Scientific and laboratory Administration expenses Total 18%

2019 ($)

2018 ($)

2017 ($)

40,238,073

38,950,957

36,222,463

9,058,817

8,806,847

6,922,922

49,296,890

47,757,804

43,145,385

30 | HUDSON INSTITUTE OF MEDICAL RESEARCH

2019 Publications BOOK CHAPTERS

JOURNAL ARTICLES

1. Algar E (2019) Germline epigenetic testing of imprinting disorders in a diagnostic setting. In Clinical Epigenetics. Hesson LB, Pritchard AL, eds. Singapore: Springer. pp 193-215.

1. Abi Nahed R, Reynaud D, Borg AJ, Traboulsi W, Wetzel A, Sapin V, Brouillet S, Dieudonne MN, Dakouane-Giudicelli M, Benharouga M, Murthi P, Alfaidy N (2019) NLRP7 is increased in human idiopathic fetal growth restriction and plays a critical role in trophoblast differentiation. J Mol Med (Berl) 97:355-367.

12. Bekkering S, Limawan AP, Nguyen MU, Widiasmoko LK, Lu H, Pepe S, Cheung MM, Menheniott TR, Wallace MJ, Burgner DP, Moss TJ (2019) Postnatal inflammation following intrauterine inflammation exacerbates the development of atherosclerosis in ApoE-/mice. Clin Sci (Lond) 133:1185-1196.

2. Aldahhan RA, Stanton PG, Ludlow H, de Kretser DM, Hedger MP (2019) Acute heat-treatment disrupts inhibin-related protein production and gene expression in the adult rat testis. Mol Cell Endocrinol 498:110546.

13. Bellofiore N, Cousins F, Temple-Smith P, Evans J (2019) Altered exploratory behaviour and increased food intake in the spiny mouse before menstruation: A unique pre-clinical model for examining premenstrual syndrome. Hum Reprod 34:308-322.

2. Creed S, McKenzie M (2019) Measurement of mitochondrial membrane potential with the fluorescent dye tetramethylrhodamine methyl ester (TMRM). In Cancer Metabolism. Haznadar M, ed. Methods in Molecular Biology. New York, NY: Humana Press. Vol 1928, pp 69-76. 3. Fuller PJ, Yang J, Young MJ (2019) Mechanisms of mineralocorticoid receptor signaling. In Vitamins and Hormones. 1st Edn. Litwack G, ed. Academic Press Inc. Vol 109, pp 37-68. 4. Funder JW (2019) Aldosterone research: 65 years, and counting. In Vitamins and Hormones. 1st Edn. Litwack G, ed. Academic Press Inc. Vol 109, pp 1-15. 5. Funder JW (2019) Primary aldosteronism: Present and future. In Vitamins and Hormones. 1st Edn. Litwack G, ed. Academic Press Inc. Vol 109, pp 285-302. 6. Ng GZ, Solomatina A, van Driel IR, Hartland EL (2019) The mouse as a model for pulmonary Legionella infection. In Legionella. Buchrieser C, Hilbi H, eds. Methods in Molecular Biology. New York, NY: Humana Press. Vol 1921, pp 399-417. 7. Nixon GM (2019) Treatment of medical (Sleep breathing disorders, restless legs syndrome, periodic limb movement disorder, and narcolepsy) sleep problems in ADHD. In Sleep and ADHD. Hiscock H, Sciberras E, eds. Academic Press Inc. pp 237-255. 8. Southwick G, Paddle A (2019) Upper Arms: Rejuvenation of the arm. In Aesthetic Surgery Techniques: A Case-Based Approach. 1st Edn. Frame J, Bagheri S, Smith Jr, D, Khan H, eds. Elsevier. pp 277-282. 9. Srirattana K, St John JC (2019) Transmission of dysfunctional mitochondrial DNA and its implications for mammalian reproduction. In Cellular and Molecular Basis of Mitochondrial Inheritance. Sutovsky P, ed. Advances in Anatomy, Embryology and Cell Biology. Switzerland: Springer. Vol 231, pp 75-103. 10. Weiner GM, Hooper SB, Davis PG, Wyckoff MH (2019) Respiratory and cardiovascular support in the delivery room. In The Newborn Lung. 3rd Edn. Bancalari E, ed. Philadelphia, PA: Elsevier. pp 173-195. 11. Ying L, Ferrero RL (2019) Role of NOD1 and ALPK1/TIFA signalling in innate immunity against Helicobacter pylori infection. In Molecular Mechanisms of Inflammation: Induction, Resolution and Escape by Helicobacter pylori. Backert S, ed. Current Topics in Microbiology and Immunology. Switzerland: Springer. Vol 421, pp 159-177. 12. Young MJ, Adler GK (2019) Aldosterone, the mineralocorticoid receptor and mechanisms of cardiovascular disease. In Vitamins and Hormones. 1st Edn. Litwack G, ed. Academic Press Inc. Vol 109, pp 105-131.

3. Alexiadis M, Rowley SM, Chu S, Leung DTH, Stewart CJR, Amarasinghe KC, Campbell IG, Fuller PJ (2019) Mutational landscape of ovarian adult granulosa cell tumors from whole exome and targeted TERT promoter sequencing. Mol Cancer Res 17:177-185. 4. Allen J, King R, Goergen SK, Melder A, Neeman N, Hadley A, Hutchinson AM (2019) Semistructured interviews regarding patients’ perceptions of choosing wisely and shared decision-making: An Australian study. BMJ Open 9:e031831. 5. Allison BJ, LaRosa DA, Barton SK, Hooper SB, Zahra VA, Tolcos M, Chan KY, Barbuto J, Inocencio IM, Moss TJ, Polglase GR (2019) Dose-dependent exacerbation of ventilation-induced lung injury by erythropoietin in preterm newborn lambs. J Appl Physiol (1985) 126:44-50. 6. Almeida A, Mitchell AL, Boland M, Forster SC, Gloor GB, Tarkowska A, Lawley TD, Finn RD (2019) A new genomic blueprint of the human gut microbiota. Nature 568:499-504. 7. Amberg BJ, Hodges RJ, Kashyap AJ, Skinner SM, Rodgers KA, McGillick EV, Deprest JA, Hooper SB, Crossley KJ, DeKoninck PLJ (2019) Physiological effects of partial amniotic carbon dioxide insufflation with cold, dry vs heated, humidified gas in a sheep model. Ultrasound Obstet Gynecol 53:340-347. 8. Atik A, De Matteo R, Boomgardt M, Rees S, Harding R, Cheong J, Rana S, Crossley K, Tolcos M (2019) Impact of high-dose caffeine on the preterm ovine cerebrum and cerebellum. Front Physiol 10:990. 9. Baker EK, Malhotra A, Lim R, Jacobs SE, Hooper SB, Davis PG, Wallace EM (2019) Human amnion cells for the prevention of bronchopulmonary dysplasia: A protocol for a phase I dose escalation study. BMJ Open 9:e026265. 10. Balic J, Garama DJ, Saad M, Yu L, West A, West AJ, Livis T, Bhathal PS, Gough DJ, Jenkins BJ (2019) Serine-phosphorylated STAT3 promotes tumorigenesis via modulation of RNA polymerase transcriptional activity. Cancer Res 79:5272-5287. 11. Baxter M, Ruane L, Phyland D, Leahy E, Heke E, Lau KK, Low K, Hamza K, MacDonald M, Bardin PG (2019) Multidisciplinary team clinic for vocal cord dysfunction directs therapy and significantly reduces healthcare utilization. Respirology 24:758-764.

14. Bernasochi GB, Bell JR, Simpson ER, Delbridge LMD, Boon WC (2019) Impact of estrogens on the regulation of white, beige, and brown adipose tissue depots. Compr Physiol 9:457-475. 15. Berry MJ, Schlegel M, Kowalski GM, Bruce CR, Callahan DL, Davies-Tuck ML, Dickinson H, Goodson A, Slocombe A, Snow RJ, Walker DW, Ellery SJ (2019) UNICORN Babies: Understanding circulating and cerebral creatine levels of the preterm infant. An observational study protocol. Front Physiol 10:142. 16. Bilandzic M, Rainczuk A, Green E, Fairweather N, Jobling TW, Plebanski M, Stephens AN (2019) Keratin-14 (KRT14) positive leader cells mediate mesothelial clearance and invasion by ovarian cancer cells. Cancers 11:1228. 17. Bracken K, Hague W, Keech A, Conway A, Handelsman DJ, Grossmann M, Jesudason D, Stuckey B, Yeap BB, Inder W, Allan C, McLachlan R, Robledo KP, Wittert G (2019) Recruitment of men to a multi-centre diabetes prevention trial: An evaluation of traditional and online promotional strategies. Trials 20:366. 18. Bracken K, Keech A, Hague W, Allan C, Conway A, Daniel M, Gebski V, Grossmann M, Handelsman DJ, Inder WJ, Jenkins A, McLachlan R, Robledo KP, Stuckey B, Yeap BB, Wittert G (2019) A high-volume, low-cost approach to participant screening and enrolment: Experiences from the T4DM diabetes prevention trial. Clin Trials 16:589-598. 19. Bracken K, Keech A, Hague W, Kirby A, Robledo KP, Allan C, Conway A, Daniel M, Gebski V, Grossmann M, Handelsman DJ, Inder W, Jenkins A, McLachlan R, Stuckey B, Yeap BB, Wittert G (2019) Telephone call reminders did not increase screening uptake more than SMS reminders: A recruitment study within a trial. J Clin Epidemiol 112:45-52. 20. Brockwell NK, Rautela J, Owen KL, Gearing LJ, Deb S, Harvey K, Spurling A, Zanker D, Chan CL, Cumming HE, Deng N, Zakhour JM, Duivenvoorden HM, Robinson T, Harris M, White M, Fox J, Ooi C, Kumar B, Thomson J, Potasz N, Swarbrick A, Hertzog PJ, Molloy TJ, Toole SO, Ganju V, Parker BS (2019) Tumor inherent interferon regulators as biomarkers of long-term chemotherapeutic response in TNBC. NPJ Precis Oncol 3:21.

ANNUAL REPORT 2019 | 31

21. Brouwer E, Knol R, Vernooij ASN, van den Akker T, Vlasman PE, Klumper F, DeKoninck P, Polglase GR, Hooper SB, Te Pas AB (2019) Physiological-based cord clamping in preterm infants using a new purpose-built resuscitation table: A feasibility study. Arch Dis Child Fetal Neonatal Ed 104:F396-F402. 22. Bryan ER, McLachlan RI, Rombauts L, Katz DJ, Yazdani A, Bogoevski K, Chang C, Giles ML, Carey AJ, Armitage CW, Trim LK, McLaughlin EA, Beagley KW (2019) Detection of chlamydia infection within human testicular biopsies. Hum Reprod 34:1891-1898. 23. Bui CB, Kolodziej M, Lamanna E, Elgass K, Sehgal A, Rudloff I, Schwenke DO, Tsuchimochi H, Kroon MAGM, Cho SX, Maksimenko A, Cholewa M, Berger PJ, Young MJ, Bourke JE, Pearson JT, Nold MF, Nold-Petry CA (2019) Interleukin-1 receptor antagonist protects newborn mice against pulmonary hypertension. Front Immunol 10:1480. 24. Catford SR, O’Bryan MK, McLachlan RI, Delatycki MB, Rombauts L (2019) Germ cell arrest associated with aSETX mutation in ataxia oculomotor apraxia type 2. Reprod Biomed Online 38:961-965. 25. Chang KTE, Tay AZE, Kuick CH, Chen H, Algar E, Taubenheim N, Campbell J, Mechinaud F, Campbell M, Super L, Chantranuwat C, Yuen ST, Chan JKC, Chow CW (2019) ALK-positive histiocytosis: An expanded clinicopathologic spectrum and frequent presence of KIF5B-ALK fusion. Mod Pathol 32:598-608. 26. Cheasley D, Wakefield MJ, Ryland GL, Allan PE, Alsop K, Amarasinghe KC, Ananda S, Anglesio MS, Au-Yeung G, Bohm M, Bowtell DDL, Brand A, Chenevix-Trench G, Christie M, Chiew YE, Churchman M, DeFazio A, Demeo R, Dudley R, Fairweather N, Fedele CG, Fereday S, Fox SB, Gilks CB, Gourley C, Hacker NF, Hadley AM, Hendley J, Ho GY, Hughes S, Hunstman DG, Hunter SM, Jobling TW, Kalli KR, Kaufmann SH, Kennedy CJ, Kobel M, Le Page C, Li J, Lupat R, McNally OM, McAlpine JN, Mes-Masson AM, Mileshkin L, Provencher DM, Pyman J, Rahimi K, Rowley SM, Salazar C, Samimi G, Saunders H, Semple T, Sharma R, Sharpe AJ, Stephens AN, Thio N, Torres MC, Traficante N, Xing Z, Zethoven M, Antill YC, Scott CL, Campbell IG, Gorringe KL (2019) The molecular origin and taxonomy of mucinous ovarian carcinoma. Nat Commun 10:3935. 27. Chen YH, Li L, Chen W, Liu ZB, Ma L, Gao XX, He JL, Wang H, Zhao M, Yang YY, Xu X (2019) Pre-pregnancy underweight and obesity are positively associated with small-for-gestational-age infants in a Chinese population. Sci Rep 9:15544. 28. Chen Z, Liu X, Li F, Li C, Marquez-Lago T, Leier A, Akutsu T, Webb GI, Xu D, Smith AI, Li L, Chou KC, Song J (2019) Large-scale comparative assessment of computational predictors for lysine post-translational modification sites. Brief Bioinform 20:2267-2290.

29. Cho KHT, Wassink G, Galinsky R, Xu B, Mathai S, Dhillon SK, van den Heuij LG, Davidson JO, Weaver-Mikaere L, Bennet L, Gunn AJ, Fraser M (2019) Protective effects of delayed intraventricular TLR7 agonist administration on cerebral white and gray matter following asphyxia in the preterm fetal sheep. Sci Rep 9:9562. 30. Clifton VL, McDonald M, Morrison JL, Holman SL, Lock MC, Saif Z, Meakin A, Wooldridge AL, Gatford KL, Wallace MJ, Muhlhausler BS, Bischof RJ, Moss TJM (2019) Placental glucocorticoid receptor isoforms in a sheep model of maternal allergic asthma. Placenta 83:33-36. 31. Clothier HJ, Lawrie J, Russell MA, Kelly H, Buttery JP (2019) Early signal detection of adverse events following influenza vaccination using proportional reporting ratio, Victoria, Australia. PLoS One 14:e0224702. 32. Cohen E, Baerts W, Caicedo Dorado A, Naulaers G, van Bel F, Lemmers PMA (2019) Cerebrovascular autoregulation in preterm fetal growth restricted neonates. Arch Dis Child Fetal Neonatal Ed 104:F467-F472. 33. Cole TJ, Short KL, Hooper SB (2019) The science of steroids. Semin Fetal Neonatal Med 24:170-175. 34. Cousins F, O DF, Ong YR, Breault DT, Deane JA, Gargett CE (2019) Telomerase reverse transcriptase expression in mouse endometrium during reepithelialization and regeneration in a menses-like model. Stem Cells Dev 28:1-12. 35. Cox AG, Gurusinghe S, Abd Rahman R, Leaw B, Chan ST, Mockler JC, Murthi P, Marshall SA, Lim R, Wallace EM (2019) Sulforaphane improves endothelial function and reduces placental oxidative stress in vitro. Pregnancy Hypertens 16:1-10. 36. Dawson EM, Dunne KA, Richardson EJ, Praszkier J, Alfawaz D, Woelfel S, De Paoli A, Hassan M, Henderson IR, Ferrero RL, Rossiter AE (2019) Complete genome sequence of Helicobacter pylori B128 7.13 and a single-step method for the generation of unmarked mutations. Helicobacter 24:e12587. 37. De Guingand DL, Ellery SJ, Davies-Tuck ML, Dickinson H (2019) Creatine and pregnancy outcomes, a prospective cohort study in lowrisk pregnant women: Study protocol. BMJ Open 9:e026756. 38. Dekker J, Hooper SB, Croughan MK, Crossley KJ, Wallace MJ, McGillick EV, DeKoninck PLJ, Thio M, Martherus T, Ruben G, Roehr CC, Cramer SJE, Flemmer AW, Croton L, Te Pas AB, Kitchen MJ (2019) Increasing respiratory effort with 100% oxygen during resuscitation of preterm rabbits at birth. Front Pediatr 7:427. 39. Dekker J, Hooper SB, Giera M, McGillick EV, Hutten GJ, Onland W, van Kaam AH, Te Pas AB (2019) High vs. low initial oxygen to improve the breathing effort of preterm infants at birth: Study protocol for a randomized controlled trial. Front Pediatr 7:179.

40. Dekker J, Lopriore E, Van Zanten HA, Tan RNGB, Hooper SB, Pas ABT (2019) Sedation during minimal invasive surfactant therapy: A randomised controlled trial. Arch Dis Child Fetal Neonatal Ed 104:F378-F383. 41. Dekker J, Martherus T, Lopriore E, Giera M, McGillick EV, Hutten J, van Leuteren RW, van Kaam AH, Hooper SB, te Pas AB (2019) The effect of initial high vs. low FiO2 on breathing effort in preterm infants at birth: A randomized controlled trial. Front Pediatr 7:504. 42. Dekker J, Stenning FJ, Willms LJFB, Martherus T, Hooper SB, te Pas AB (2019) Time to achieve desired fraction of inspired oxygen using a T-piece ventilator during resuscitation of preterm infants at birth. Resuscitation 136:100-104. 43. DeKoninck PLJ, Crossley KJ, Kashyap AJ, Skinner SM, Thio M, Rodgers KA, Deprest JA, Hooper SB, Hodges RJ (2019) Effects of tracheal occlusion on the neonatal cardiopulmonary transition in an ovine model of diaphragmatic hernia. Arch Dis Child Fetal Neonatal Ed 104:F609-F616. 44. Delforce SJ, Lumbers ER, Ellery SJ, Murthi P, Pringle KG (2019) Dysregulation of the placental renin–angiotensin system in human fetal growth restriction. Reproduction 158:237245. 45. Dowling JK, Tate MD, Rosli S, Bourke NM, Bitto N, Lauterbach MA, Cheung S, Ve T, Kobe B, Golenbock D, Mansell A (2019) The single nucleotide polymorphism Mal-D96N mice provide new insights into functionality of Mal in TLR immune responses. J Immunol 202:2384-2396. 46. Duszynski KM, Pratt NL, Lynch JW, Braunack-Mayer A, Taylor LK, Berry JG, Xafis V, Buttery J, Gold MS (2019) Process trumps potential public good: Better vaccine safety through linked cross-jurisdictional immunisation data in Australia. Aust N Z J Public Health 43:496-503. 47. Ellery SJ, Goss MG, Brew N, Dickinson H, Hale N, LaRosa DA, Walker DW, Wong FY (2019) Evaluation of 3K3A-activated protein C to treat neonatal hypoxic ischemic brain injury in the spiny mouse. Neurotherapeutics 16:231-243. 48. Ellery SJ, Murthi P, Davies-Tuck ML, Gatta PD, May AK, Kowalski GM, Callahan DL, Bruce CR, Alers NO, Miller SL, Erwich J, Wallace EM, Walker DW, Dickinson H, Snow RJ (2019) Placental creatine metabolism in cases of placental insufficiency and reduced fetal growth. Mol Hum Reprod 25:495-505. 49. Emmerson S, Mukherjee S, Melendez-Munoz J, Cousins F, Edwards SL, Karjalainen P, Ng M, Tan KS, Darzi S, Bhakoo K, Rosamilia A, Werkmeister JA, Gargett CE (2019) Composite mesh design for delivery of autologous mesenchymal stem cells influences mesh integration, exposure and biocompatibility in an ovine model of pelvic organ prolapse. Biomaterials 225:119495.

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50. Erlich Z, Shlomovitz I, Edry-Botzer L, Cohen H, Frank D, Wang H, Lew AM, Lawlor KE, Zhan Y, Vince JE, Gerlic M (2019) Macrophages, rather than DCs, are responsible for inflammasome activity in the GM-CSF BMDC model. Nat Immunol 20:397-406. 51. Evans J, Infusini G, McGovern J, Cuttle L, Webb A, Nebl T, Milla L, Kimble R, Kempf M, Andrews CJ, Leavesley D, Salamonsen LA (2019) Menstrual fluid factors facilitate tissue repair: Identification and functional action in endometrial and skin repair. Faseb J 33:584605. 52. Evans J, Rai A, Nguyen HPT, Poh QH, Elglass K, Simpson RJ, Salamonsen LA, Greening DW (2019) Human endometrial extracellular vesicles functionally prepare human trophectoderm model for implantation: Understanding bidirectional maternal-embryo communication. Proteomics 19:e1800423. 53. Evans J, Rai A, Nguyen HPT, Poh QH, Elglass K, Simpson RJ, Salamonsen LA, Greening DW (2019) In vitro human implantation model reveals a role for endometrial extracellular vesicles in embryo implantation: Reprogramming the cellular and secreted proteome landscapes for bidirectional fetal-maternal communication. Proteomics 19:e1800423. 54. Falcao-Tebas F, Kuang J, Arceri C, Kerris JP, Andrikopoulos S, Marin EC, McConell GK (2019) Four weeks of exercise early in life reprograms adult skeletal muscle insulin resistance caused by a paternal high-fat diet. J Physiol 597:121-136. 55. Feng SYS, Hollis JH, Samarasinghe T, Phillips DJ, Rao S, Yu VYH, Walker AM (2019) Endotoxin-induced cerebral pathophysiology: Differences between fetus and newborn. Physiol Rep 7:e13973. 56. Fletcher EK, Kanki M, Morgan J, Ray DW, Delbridge L, Fuller PJ, Clyne CD, Young M (2019) Cardiomyocyte transcription is controlled by combined mineralocorticoid receptor and circadian clock signalling. J Endocrinol 241:17–29. 57. Foreman M, Hare L, York K, Balakrishnan K, Sánchez FJ, Harte F, Erasmus J, Vilain E, Harley VR (2019) Genetic link between gender dysphoria and sex hormone signaling. J Clin Endocrinol Metab 104:390-396. 58. Forster SC, Kumar N, Anonye BO, Almeida A, Viciani E, Stares MD, Dunn M, Mkandawire TT, Zhu A, Shao Y, Pike LJ, Louie T, Browne HP, Mitchell AL, Neville BA, Finn RD, Lawley TD (2019) A human gut bacterial genome and culture collection for improved metagenomic analyses. Nat Biotechnol 37:186-192. 59. Freitag J, Bates D, Wickham J, Shah K, Huguenin L, Tenen A, Paterson K, Boyd R (2019) Adipose-derived mesenchymal stem cell therapy in the treatment of knee osteoarthritis: A randomized controlled trial. Regen Med 14:213-230. 60. Fuller PJ, Yao YZ, Jin R, He S, Martin-Fernandez B, Young MJ, Smith BJ (2019) Molecular evolution of the switch for progesterone and spironolactone from mineralocorticoid receptor agonist to antagonist. Proc Natl Acad Sci U S A 116:18578-18583.

61. Gearing LJ, Cumming HE, Chapman R, Finkel AM, Woodhouse IB, Luu K, Gould JA, Forster SC, Hertzog PJ (2019) CiiiDER: A tool for predicting and analysing transcription factor binding sites. PLoS One 14:e0215495. 62. Gerbaud P, Murthi P, Guibourdenche J, Guimiot F, Sarazin B, Evain-Brion D, Badet J, Pidoux G (2019) Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular development. Endocrinology 160:684-698. 63. Goh M, Nguyen HH, Khan NN, Milat F, Boyle JA, Vincent AJ (2019) Identifying and addressing osteoporosis knowledge gaps in women with premature ovarian insufficiency and early menopause: A mixed-methods study. Clin Endocrinol (Oxf) 91:498-507. 64. Gomez-Valero L, Rusniok C, Carson D, Mondino S, Perez-Cobas AE, Rolando M, Pasricha S, Reuter S, Demirtas J, Crumbach J, Descorps-Declere S, Hartland EL, Jarraud S, Dougan G, Schroeder GN, Frankel G, Buchrieser C (2019) More than 18,000 effectors in the Legionella genus genome provide multiple, independent combinations for replication in human cells. Proc Natl Acad Sci U S A 116:2265-2273. 65. Greenall SA, McKenzie M, Seminova E, Dolezal O, Pearce L, Bentley J, Kuchibhotla M, Chen SC, McDonald KL, Kornblum H, Endersby R, Adams TE, Johns TG (2019) Most clinical anti-EGFR antibodies do not neutralize both wtEGFR and EGFRvIII activation in glioma. Neuro Oncol 21:1016–1027. 66. Griffiths M, Van Sinderen M, Rainczuk K, Dimitriadis E (2019) miR-29c overexpression and COL4A1 downregulation in infertile human endometrium reduces endometrial epithelial cell adhesive capacity in vitro implying roles in receptivity. Sci Rep 9:8644. 67. Halliday J, Lewis S, Kennedy J, Burgner DP, Juonala M, Hammarberg K, Amor DJ, Doyle LW, Saffery R, Ranganathan S, Welsh L, Cheung M, McBain J, Hearps SJC, McLachlan R (2019) Health of adults aged 22 to 35 years conceived by assisted reproductive technology. Fertil Steril 112:130-139. 68. Hamblin PS, Sheehan PM, Allan C, Houlihan CA, Lu ZX, Forehan SP, Topliss DJ, Gilfillan C, Krishnamurthy B, Renouf D, Sztal-Mazer S, Varadarajan S (2019) Subclinical hypothyroidism during pregnancy: The Melbourne public hospitals consensus. Intern Med J 49:994-1000. 69. Han ML, Zhu Y, Creek DJ, Lin YW, Gutu AD, Hertzog P, Purcell T, Shen HH, Moskowitz SM, Velkov T, Li J (2019) Comparative metabolomics and transcriptomics reveal multiple pathways associated with polymyxin killing in Pseudomonas aeruginosa. mSystems 4:e00149-00118. 70. Han TS, Voon DC, Oshima H, Nakayama M, Echizen K, Sakai E, Yong ZWE, Murakami K, Yu L, Minamoto T, Ock CY, Jenkins BJ, Kim SJ, Yang HK, Oshima M (2019) Interleukin 1 up-regulates microRNA 135b to promote inflammation-associated gastric carcinogenesis in mice. Gastroenterology 156:1140-1155 e1144.

71. Hapangama DK, Drury J, Da Silva L, AlLamee H, Earp A, Valentijn AJ, Edirisinghe DP, Murray PA, Fazleabas AT, Gargett CE (2019) Abnormally located SSEA1+/SOX9+ endometrial epithelial cells with a basalis-like phenotype in the eutopic functionalis layer may play a role in the pathogenesis of endometriosis. Hum Reprod 34:56-68. 72. Hart RJ, Doherty DA, Mori TA, Adams LA, Huang RC, Minaee N, Handelsman DJ, McLachlan R, Norman RJ, Dickinson JE, Olynyk JK, Beilin LJ (2019) Features of the metabolic syndrome in late adolescence are associated with impaired testicular function at 20 years of age. Hum Reprod 34:389-402. 73. Hayman TJ, Hsu AC, Kolesnik TB, Dagley LF, Willemsen J, Tate MD, Baker PJ, Kershaw NJ, Kedzierski L, Webb AI, Wark PA, Kedzierska K, Masters SL, Belz GT, Binder M, Hansbro PM, Nicola NA, Nicholson SE (2019) RIPLET, and not TRIM25, is required for endogenous RIG-I-dependent antiviral responses. Immunol Cell Biol 97:840-852. 74. Hernandez N, Bucciol G, Moens L, Le Pen J, Shahrooei M, Goudouris E, Shirkani A, Changi-Ashtiani M, Rokni-Zadeh H, Sayar EH, Reisli I, Lefevre-Utile A, Zijlmans D, Jurado A, Pholien R, Drutman S, Belkaya S, Cobat A, Boudewijns R, Jochmans D, Neyts J, Seeleuthner Y, Lorenzo-Diaz L, Enemchukwu C, Tietjen I, Hoffmann HH, Momenilandi M, Poyhonen L, Siqueira MM, de Lima SMB, de Souza Matos DC, Homma A, Maia MLS, da Costa Barros TA, de Oliveira PMN, Mesquita EC, Gijsbers R, Zhang SY, Seligman SJ, Abel L, Hertzog P, Marr N, Martins RM, Meyts I, Zhang Q, MacDonald MR, Rice CM, Casanova JL, Jouanguy E, Bossuyt X (2019) Inherited IFNAR1 deficiency in otherwise healthy patients with adverse reaction to measles and yellow fever live vaccines. J Exp Med 216:2057-2070. 75. Hogg K, Rizio T, Manocha R, McLachlan RI, Hammarberg K (2019) Men’s preconception health care in Australian general practice: GPs’ knowledge, attitudes and behaviours. Aust J Prim Health 25:353-358. 76. Hokke S, de Zoysa N, Carr BL, Abruzzo V, Coombs PR, Allan CA, East C, Ingelfinger JR, Puelles VG, Black MJ, Ryan D, Armitage JA, Wallace EM, Bertram JF, Cullen-McEwen LA (2019) Normal foetal kidney volume in offspring of women treated for gestational diabetes. Endocrinol Diabetes Metab 2:e00091. 77. Hooper SB, Roberts C, Dekker J, Te Pas AB (2019) Issues in cardiopulmonary transition at birth. Semin Fetal Neonatal Med 24:101033. 78. Inocencio IM, Polglase GR, Miller SL, Sehgal A, Sutherland A, Mihelakis J, Li A, Allison BJ (2019) Effects of maternal sildenafil treatment on vascular function in growth-restricted fetal sheep. Arterioscler Thromb Vasc Biol 39:731–740. 79. Jones SA, Cantsilieris S, Fan H, Cheng Q, Russ BE, Tucker EJ, Harris J, Rudloff I, Nold M, Northcott M, Dankers W, Toh AEJ, White SJ, Morand EF (2019) Rare variants in non-coding regulatory regions of the genome that affect gene expression in systemic lupus erythematosus. Sci Rep 9:15433.

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80. Kadife E, Chan E, Luwor R, Kannourakis G, Findlay J, Ahmed N (2019) Paclitaxel-induced Src activation is inhibited by dasatinib treatment, independently of cancer stem cell properties, in a mouse model of ovarian cancer. Cancers (Basel) 11:E243. 81. Kamlin COF, Schmolzer GM, Dawson JA, McGrory L, O’Shea J, Donath SM, Lorenz L, Hooper SB, Davis PG (2019) A randomized trial of oropharyngeal airways to assist stabilization of preterm infants in the delivery room. Resuscitation 144:106-114. 82. Kashyap AJ, Crossley KJ, DeKoninck PLJ, Rodgers KA, Thio M, Skinner SM, Deprest JA, Hooper SB, Hodges RJ (2019) Neonatal cardiopulmonary transition in an ovine model of congenital diaphragmatic hernia. Arch Dis Child Fetal Neonatal Ed 104:F617-F623. 83. Kashyap AJ, DeKoninck PLJ, Rodgers KA, Thio M, McGillick EV, Amberg BJ, Skinner SM, Moxham AM, Russo FM, Deprest JA, Hooper SB, Crossley KJ, Hodges RJ (2019) Antenatal sildenafil treatment improves neonatal pulmonary hemodynamics and gas exchange in lambs with diaphragmatic hernia. Ultrasound Obstet Gynecol 54:506-516. 84. Kauerhof AC, Nicolas N, Bhushan S, Wahle E, Loveland KA, Fietz D, Bergmann M, Groome NP, Kliesch S, Schuppe HC, Pilatz A, Meinhardt A, Hedger MP, Fijak M (2019) Investigation of activin A in inflammatory responses of the testis and its role in the development of testicular fibrosis. Hum Reprod 34:1536-1550. 85. Khanmohammadi M, Golshahi H, Saffarian Z, Montazeri S, Khorasani S, Kazemnejad S (2019) Repair of osteochondral defects in rabbit knee using menstrual blood stem cells encapsulated in fibrin glue: A good stem cell candidate for the treatment of osteochondral defects. Tissue Eng Regen Med 16:311-324. 86. Kinnear S, Salamonsen LA, Francois M, Harley V, Evans J (2019) Uterine SOX17: A key player in human endometrial receptivity and embryo implantation. Sci Rep 9:15495. 87. Kiriakova V, Cooray SD, Yeganeh L, Somarajah G, Milat F, Vincent AJ (2019) Management of bone health in women with premature ovarian insufficiency: Systematic appraisal of clinical practice guidelines and algorithm development. Maturitas 128:70-80. 88. Klein B, Pant S, Bhushan S, Kautz J, Rudat C, Kispert A, Pilatz A, Wijayarathna R, Middendorff R, Loveland KL, Hedger MP, Meinhardt A (2019) Dexamethasone improves therapeutic outcomes in a preclinical bacterial epididymitis mouse model. Hum Reprod 34:1195-1205. 89. Knarston IM, Robevska G, van den Bergen JA, Eggers S, Croft B, Yates J, Hersmus R, Looijenga LHJ, Cameron FJ, Monhike K, Ayers KL, Sinclair AH (2019) NR5A1 gene variants repress the ovarian-specific WNT signalling pathway in 46,XX disorders of sex development patients. Hum Mutat 40:207-216. 90. Knol R, Brouwer E, Klumper FJCM, van den Akker T, DeKoninck P, Hutten GJ, Lopriore E, van Kaam AH, Polglase GR, Reiss IKM, Hooper SB, Pas AB (2019) Effectiveness of stabilization of preterm infants with intact umbilical cord using a purpose-built resuscitation table-study protocol for a randomized controlled trial. Front Pediatr 7:134.

91. Krishnan SM, Ling YH, Huuskes BM, Ferens D, Saini N, Chan CT, Diep H, Kett MM, Samuel CS, Kemp-Harper BK, Robertson AAB, Cooper MA, Peter K, Latz E, Mansell A, Sobey CG, Drummond GR, Vinh A (2019) Pharmacological inhibition of the NLRP3 inflammasome reduces blood pressure, renal damage, and dysfunction in salt-sensitive hypertension. Cardiovasc Res 115:776-787. 92. Kudipudi PK, Galuska SP, Dietze R, Scheiner-Bobis G, Loveland KL, Konrad L (2019) Betaglycan (TβRIII) is a key factor in TGF-β2 signaling in prepubertal rat sertoli cells. Int J Mol Sci 20:6214. 93. Kuk N, Hodge A, Sun Y, Correia J, Alhomrani M, Samuel C, Moore G, Lim R, Sievert W (2019) Human amnion epithelial cells and their soluble factors reduce liver fibrosis in murine non-alcoholic steatohepatitis. J Gastroenterol Hepatol 34:1441-1449. 94. Kumar N, Browne HP, Viciani E, Forster SC, Clare S, Harcourt K, Stares MD, Dougan G, Fairley DJ, Roberts P, Pirmohamed M, Clokie MRJ, Jensen MBF, Hargreaves KR, Ip M, Wieler LH, Seyboldt C, Noren T, Riley TV, Kuijper EJ, Wren BW, Lawley TD (2019) Adaptation of host transmission cycle during Clostridium difficile speciation. Nat Genet 51:1315-1320. 95. Kuypers K, Lamberska T, Martherus T, Dekker J, Bohringer S, Hooper SB, Plavka R, Te Pas AB (2019) The effect of a face mask for respiratory support on breathing in preterm infants at birth. Resuscitation 144:178-184. 96. Langston-Cox AG, Marshall SA, Palmer KR, Wallace EM (2019) Prolong: A double-blind randomised placebo-controlled trial of broccoli sprout extract in women with early onset preeclampsia. A clinical trial protocol. BMJ Open 9:e027493. 97. Laurent I, Astere M, Zheng F, Chen X, Yang J, Cheng Q, Li Q (2019) Adrenal venous sampling with or without adrenocorticotropic hormone stimulation: A meta-analysis. J Clin Endocr Metab 104:1060-1068. 98. Le Nours J, Gherardin NA, Ramarathinam SH, Awad W, Wiede F, Gully BS, Khandokar Y, Praveena T, Wubben JM, Sandow JJ, Webb AI, von Borstel A, Rice MT, Redmond SJ, Seneviratna R, Sandoval-Romero ML, Li S, Souter MNT, Eckle SBG, Corbett AJ, Reid HH, Liu L, Fairlie DP, Giles EM, Westall GP, Tothill RW, Davey MS, Berry R, Tiganis T, McCluskey J, Pellicci DG, Purcell AW, Uldrich AP, Godfrey DI, Rossjohn J (2019) A class of gd T cell receptors recognize the underside of the antigen-presenting molecule MR1. Science 366:1522-1527. 99. Lee E, Lim Z, Malhotra A (2019) Thrombocytopenia in well small for gestational age neonates. Blood Coagul Fibrinolysis 30:104-110. 100. Lee J, Pinares-Garcia P, Loke H, Ham S, Vilain E, Harley VR (2019) Sex-specific neuroprotection by inhibition of the Y-chromosome gene, SRY, in experimental Parkinson’s disease. Proc Natl Acad Sci U S A 116:1657716582. 101. Legrand JMD, Chan A-L, La HM, Rossello FJ, Änkö M-L, Fuller-Pace FV, Hobbs RM (2019) DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia. Nat Commun 10:2278.

102. Leong TL, Gayevskiy V, Steinfort DP, De Massy MR, Gonzalez-Rajal A, Marini KD, Stone E, Chin V, Havryk A, Plit M, Irving LB, Jennings BR, McCloy RA, Jayasekara WSN, Alamgeer M, Boolell V, Field A, Russell PA, Kumar B, Gough DJ, Szczepny A, Ganju V, Rossello FJ, Cain JE, Papenfuss AT, Asselin-Labat ML, Cowley MJ, Watkins DN (2019) Deep multi-region whole-genome sequencing reveals heterogeneity and gene-by-environment interactions in treatment-naive, metastatic lung cancer. Oncogene 38:1661-1675. 103. Lepletier A, Hun ML, Hammett MV, Wong K, Naeem H, Hedger M, Loveland K, Chidgey AP (2019) Interplay between follistatin, activin A, and BMP4 signaling regulates postnatal thymic epithelial progenitor cell differentiation during aging. Cell Rep 27:3887-3901 e3884. 104. Leung DTH, Nguyen T, Oliver EM, Matti J, Alexiadis M, Silke J, Jobling TW, Fuller PJ, Chu S (2019) Combined PPARg activation and XIAP inhibition as a potential therapeutic strategy for ovarian granulosa cell tumors. Mol Cancer Ther 18:364-375. 105. Leung DTH, Rainczuk A, Nguyen T, Stephens A, Silke J, Fuller PJ, Chu S (2019) Targeting XIAP and PPARI in granulosa cell tumors alters metabolic signaling. J Proteome Res 18:1691-1702. 106. Li A, Wilson S, Fitzpatrick I, Barabadi M, Chan ST, Krause M, Kusuma GD, James D, Lim R (2019) Automated counterflow centrifugal system for small-scale cell processing. J Vis Exp 154:e60423. 107. Li X, Li Y, Ahammed GJ, Zhang XN, Ying L, Zhang L, Yan P, Zhang LP, Li QY, Han WY (2019) RBOH1-dependent apoplastic H2O2 mediates epigallocatechin-3-gallate-induced abiotic stress tolerance in Solanum lycopersicum L. Environ Exp Bot 161:357-366. 108. Lidbury BA, Kita B, Richardson AM, Lewis DP, Privitera E, Hayward S, de Kretser D, Hedger M (2019) Rethinking ME/CFS diagnostic reference intervals via machine learning, and the utility of activin B for defining symptom severity. Diagnostics (Basel) 9:79. 109. Lim KZ, Daly C, Brown J, Goldschlager T (2019) Dynamic posture-related preoperative pain as a single clinical criterion in patient selection for extreme lateral interbody fusion without direct decompression. Global Spine J 9:575-582. 110. Lin YW, Han ML, Zhao J, Zhu Y, Rao G, Forrest A, Song J, Kaye KS, Hertzog P, Purcell A, Creek D, Zhou QT, Velkov T, Li J (2019) Synergistic combination of polymyxin B and enrofloxacin induced metabolic perturbations in extensive drug-resistant Pseudomonas aeruginosa. Front Pharmacol 10:1146. 111. Liu X, D’Cruz AA, Hansen J, Croker BA, Lawlor KE, Sims NA, Wicks IP (2019) Deleting suppressor of cytokine signaling-3 in chondrocytes reduces bone growth by disrupting mitogen-activated protein kinase signaling. Osteoarthritis Cartilage 27:1557-1563. 112. Liu YD, Yu L, Ying L, Balic J, Gao H, Deng NT, West A, Yan F, Ji CB, Gough D, Tan P, Jenkins BJ, Li JK (2019) Toll-like receptor 2 regulates metabolic reprogramming in gastric cancer via superoxide dismutase 2. Int J Cancer 144:3056-3069.

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113. Lobachevsky PN, Ventura J, Giannakandropoulou L, Forrester H, Palazzolo JS, Haynes NM, Stevenson AW, Hall CJ, Mason J, Pollakis G, Pateras IS, Gorgoulis V, Terzoudi GI, Hamilton JA, Sprung CN, Georgakilas AG, Martin OA (2019) A functional immune system is required for the systemic genotoxic effects of localized irradiation. Int J Radiat Oncol Biol Phys 103:1184-1193. 114. Loke H, Rainczuk K, Dimitriadis E (2019) MicroRNA biogenesis machinery is dysregulated in the endometrium of infertile women suggesting a role in receptivity and infertility. J Histochem Cytochem 67:589-599. 115. Low MSY, Brodie EJ, Fedele PL, Liao Y, Grigoriadis G, Strasser A, Kallies A, Willis SN, Tellier J, Shi W, Gabriel S, O’Donnell K, Pitt C, Nutt SL, Tarlinton D (2019) IRF4 activity is required in established plasma cells to regulate gene transcription and mitochondrial homeostasis. Cell Rep 29:2634-2645.e2635. 116. Ma JZ, Ng WC, Zappia L, Gearing LJ, Olshansky M, Pham K, Cheong K, Hsu A, Turner SJ, Wijburg O, Londrigan SL, Brooks AG, Reading PC (2019) Unique transcriptional architecture in airway epithelial cells and macrophages shapes distinct responses following influenza virus infection ex vivo. J Virol 93:e01986-01918. 117. MacDonald MI, Osadnik CR, Bulfin L, Hamza K, Leong P, Wong A, King PT, Bardin PG (2019) Low and high blood eosinophil counts as biomarkers in hospitalized acute exacerbations of COPD. Chest 156:92-100. 118. Major AT, Hogarth CA, Young JC, Kurihara Y, Jans DA, Loveland KL (2019) Dynamic paraspeckle component localisation during spermatogenesis. Reproduction 158:267-280. 119. Malhotra A, Miller SL, Jenkin G, Hooper SB, Allison BJ, Sozo F, Zahra V, Sehgal A, Polglase GR (2019) Fetal growth restriction is associated with an altered cardiopulmonary and cerebral hemodynamic response to surfactant therapy in preterm lambs. Pediatr Res 86:47-54. 120. Malhotra A, Sepehrizadeh T, Dhollander T, Wright D, Castillo-Melendez M, Sutherland AE, Pham Y, Ditchfield M, Polglase GR, de Veer M, Jenkin G, Pannek K, Shishegar R, Miller SL (2019) Advanced MRI analysis to detect white matter brain injury in growth restricted newborn lambs. NeuroImage-Clin. 24:101991. 121. Malikiwi AI, Lee YM, Davies-Tuck M, Wong FY (2019) Postnatal nutritional deficit is an independent predictor of bronchopulmonary dysplasia among extremely premature infants born at or less than 28 weeks gestation. Early Hum Dev 131:29-35. 122. Martherus T, Oberthuer A, Dekker J, Kirchgaessner C, van Geloven N, Hooper SB, Kribs A, Te Pas AB (2019) Comparison of two respiratory support strategies for stabilization of very preterm infants at birth: A matchedpairs analysis. Front Pediatr 7:3. 123. Massillo C, Dalton GN, Porretti J, Scalise GD, Farre PL, Piccioni F, Secchiari F, Pascuali N, Clyne C, Gardner K, De Luca P, De Siervi A (2019) CTBP1/CYP19A1/Estradiol axis together with adipose tissue impacts over prostate cancer growth associated to metabolic syndrome. Int J Cancer 144:1115-1127.

124. McDonald CA, Djuliannisaa Z, Petraki M, Paton MCB, Penny TR, Sutherland AE, Castillo-Melendez M, Novak I, Jenkin G, Fahey MC, Miller SL (2019) Intranasal delivery of mesenchymal stromal cells protects against neonatal hypoxic-ischemic brain injury. Int J Mol Sci 20:2449. 125. McGillick EV, Davies IM, Hooper SB, Kerr LT, Thio M, DeKoninck P, Yamaoka S, Hodges R, Rodgers K, Zahra VA, Moxham AM, Kashyap AJ, Crossley KJ (2019) Effect of lung hypoplasia on the cardiorespiratory transition in newborn lambs. J Appl Physiol 127:568-578. 126. Menkhorst EM, Van Sinderen M, Correia J, Dimitriadis E (2019) Trophoblast function is altered by decidual factors in gestational-dependant manner. Placenta 80:8-11. 127. Mesfin YM, Cheng AC, Tran AHL, Buttery J (2019) Positive predictive value of ICD-10 codes to detect anaphylaxis due to vaccination: A validation study. Pharmacoepidemiol Drug Saf 28:1353-1360. 128. Mian Q, Cheung PY, O’Reilly M, Barton SK, Polglase GR, Schmolzer GM (2019) Impact of delivered tidal volume on the occurrence of intraventricular haemorrhage in preterm infants during positive pressure ventilation in the delivery room. Arch Dis Child Fetal Neonatal Ed 104:F57-F62. 129. Modak JK, Tikhomirova A, Gorrell RJ, Rahman MM, Kotsanas D, Korman TM, Garcia-Bustos J, Kwok T, Ferrero RL, Supuran CT, Roujeinikova A (2019) Anti-Helicobacter pylori activity of ethoxzolamide. J Enzyme Inhib Med Chem 34:1660-1667. 130. Mukherjee S, Darzi S, Rosamilia A, Kadam V, Truong Y, Werkmeister JA, Gargett CE (2019) Blended nanostructured degradable mesh with endometrial mesenchymal stem cells promotes tissue integration and anti-inflammatory response in vivo for pelvic floor application. Biomacromolecules 20:454-468. 131. Newson JP, Scott NE, Yeuk Wah Chung I, Wong Fok Lung T, Giogha C, Gan J, Wang N, Strugnell R, Brown NF, Cygler M, Pearson JS, Hartland EL (2019) Salmonella effectors SseK1 and SseK3 target death domain proteins in the TNF and TRAIL signaling pathways. Mol Cell Proteomics 18:1138-1156. 132. Nguyen QN, Zerafa N, Liew SH, Findlay JK, Hickey M, Hutt KJ (2019) Cisplatin- and cyclophosphamide-induced primordial follicle depletion is caused by direct damage to oocytes. Mol Hum Reprod 25:433-444. 133. Nguyen TA, Smith BRC, Elgass KD, Creed SJ, Cheung S, Tate MD, Belz GT, Wicks IP, Masters SL, Pang KC (2019) SIDT1 localizes to endolysosomes and mediates double-stranded RNA transport into the cytoplasm. J Immunol 202:3483-3492. 134. Novakovic B, Lewis S, Halliday J, Kennedy J, Burgner DP, Czajko A, Kim B, Sexton-Oates A, Juonala M, Hammarberg K, Amor DJ, Doyle LW, Ranganathan S, Welsh L, Cheung M, McBain J, McLachlan R, Saffery R (2019) Assisted reproductive technologies are associated with limited epigenetic variation at birth that largely resolves by adulthood. Nat Commun 10:3922.

135. Nowotny BM, Loh E, Lorenz K, Wallace EM (2019) Sharing the pain: Lessons from missed opportunities for healthcare improvement from patient complaints and litigation in the Australian health system. Aust Health Rev 43:382-391 136. Oppy CC, Jebeli L, Kuba M, Oates CV, Strugnell R, Edgington-Mitchell LE, Valvano MA, Hartland EL, Newton HJ, Scott NE (2019) Loss of O-Linked protein glycosylation in Burkholderia cenocepacia impairs biofilm formation and siderophore activity and alters transcriptional regulators. mSphere 4:e0066000619. 137. Palmer KR, Mockler JC, Davies-Tuck ML, Miller SL, Goergen SK, Fahey MC, Anderson PJ, Groom KM, Wallace EM (2019) Protect-me: A parallel-group, triple blinded, placebo-controlled randomised clinical trial protocol assessing antenatal maternal melatonin supplementation for fetal neuroprotection in early-onset fetal growth restriction. BMJ Open 9:e028243. 138. Parambi A, Davies-Tuck M, Palmer KR (2019) Comparison of maternal and perinatal outcomes in women with super obesity based on planned mode of delivery. Aust N Z J Obstet Gynaecol 59:387-393. 139. Parkinson LA, Rosamilia A, Mukherjee S, Papageorgiou AW, Melendez-Munoz J, Werkmeister JA, Gargett CE, Arkwright JW (2019) A fiber-optic sensor-based device for the measurement of vaginal integrity in women. Neurourol Urodyn 38:2264-2272. 140. Paton MCB, Allison BJ, Fahey MC, Li J, Sutherland AE, Pham Y, Nitsos I, Bischof RJ, Moss TJ, Polglase GR, Jenkin G, Miller SL, McDonald CA (2019) Umbilical cord blood versus mesenchymal stem cells for inflammation-induced preterm brain injury in fetal sheep. Pediatr Res 86:165-173. 141. Paul K, Darzi S, McPhee G, Del Borgo MP, Werkmeister JA, Gargett CE, Mukherjee S (2019) 3D bioprinted endometrial stem cells on melt electrospun poly ε-caprolactone mesh for pelvic floor application promote anti-inflammatory responses in mice. Acta Biomaterialia 97:162-176. 142. Penny TR, Sutherland AE, Mihelakis JG, Paton MCB, Pham Y, Lee J, Jones NM, Jenkin G, Fahey MC, Miller SL, McDonald CA (2019) Human umbilical cord therapy improves longterm behavioral outcomes following neonatal hypoxic ischemic brain injury. Front Physiol 10:283. 143. Pillman KA, Goodall GJ, Bracken CP, Gantier MP (2019) miRNA length variation during macrophage stimulation confounds the interpretation of results: Implications for miRNA quantification by RT-qPCR. RNA 25:232-238. 144. Prier JE, Li J, Gearing LJ, Olshansky M, Sng XYX, Hertzog PJ, Turner SJ (2019) Early T-bet expression ensures an appropriate CD8+ lineage–specific transcriptional landscape after influenza A virus infection. J Immunol 203:1044-1054. 145. Pusceddu MM, Barboza M, Keogh CE, Schneider M, Stokes P, Sladek JA, Jung Danielle Kim H, Torres-Fuentes C, Goldfild LR, Gillis SE, Brust-Mascher I, Rabasa G, Wong KA, Lebrilla C, Byndloss MX, Maisonneuve C, Baumler AJ, Philpott DJ, Ferrero RL, Barrett

ANNUAL REPORT 2019 | 35

KE, Reardon C, Gareau MG (2019) Nod-like receptors are critical for gut–brain axis signalling in mice. J Physiol 597:5777-5797. 146. Rahman T, Brown AS, Hartland EL, Van Driel IR, Fung KY (2019) Plasmacytoid dendritic cells provide protection against bacterial-induced colitis. Front Immunol 10:608. 147. Rana S, Shishegar R, Quezada S, Johnston L, Walker DW, Tolcos M (2019) The subplate: A potential driver of cortical folding? Cereb Cortex 29:4697-4708. 148. Ranzil S, Ellery S, Walker DW, Vaillancourt C, Alfaidy N, Bonnin A, Borg A, Wallace EM, Ebeling PR, Erwich JJ, Murthi P (2019) Disrupted placental serotonin synthetic pathway and increased placental serotonin: Potential implications in the pathogenesis of human fetal growth restriction. Placenta 84:74-83. 149. Ratner RT, Harris A, Tsaltas J, Goyal N, Davies-Tuck M, Najjar H, Barel O (2019) An eight-year retrospective analysis of laparoscopic surgery for endometriosis, outcomes and complications in a large multicenter unit. Clin Exp Obstet Gynecol 46:699-703. 150. Richani D, Constance K, Lien S, Agapiou D, Stocker WA, Hedger MP, Ledger WL, Thompson JG, Robertson DM, Mottershead DG, Walton KL, Harrison CA, Gilchrist RB (2019) Cumulin and FSH cooperate to regulate inhibin B and activin B production by human granulosa-lutein cells in vitro. Endocrinology 160:853-862. 151. Riepsamen AH, Chan K, Lien S, Sweeten P, Donoghoe MW, Walker G, Fraison EHJ, Stocker WA, Walton KL, Harrison CA, Ledger WL, Robertson DM, Gilchrist RB (2019) Serum concentrations of oocyte-secreted factors BMP15 and GDF9 during IVF and in women with reproductive pathologies. Endocrinology 160:2298-2313. 152. Rolnik DL, Wang Y, Hyett J, Silva Costa FD, Nie G (2019) Serum podocalyxin at 11–13 weeks of gestation in the prediction of small for gestational age neonates. J Perinatol 39:784-790. 153. Rosales Nieto CA, Ferguson MB, Briegel JR, Hedger MP, Martin GB, Thompson AN (2019) Pre-pubertal growth, muscle and fat accumulation in male and female sheep—Relationships with metabolic hormone concentrations, timing of puberty and reproductive outcomes. Reprod Domest Anim 54:1596-1603. 154. Rosli S, Kirby FJ, Lawlor KE, Rainczuk K, Drummond GR, Mansell A, Tate MD (2019) Repurposing drugs targeting the P2X7 receptor to limit hyperinflammation and disease during influenza virus infection. Br J Pharmacol 176:3834-3844. 155. Royce SG, Mao W, Lim R, Kelly K, Samuel CS (2019) iPSC- and mesenchymoangioblast-derived mesenchymal stem cells provide greater protection against experimental chronic allergic airways disease compared with a clinically used corticosteroid. Faseb J 33:6402-6411. 156. Royce SG, Patel KP, Mao W, Zhu D, Lim R, Samuel CS (2019) Serelaxin enhances the therapeutic effects of human amnion epithelial cell-derived exosomes in experimental models of lung disease. Br J Pharmacol 176:2195-2208.

157. Saad MI, Alhayyani S, McLeod L, Yu L, Alanazi M, Deswaerte V, Tang K, Jarde T, Smith JA, Prodanovic Z, Tate MD, Balic JJ, Watkins DN, Cain JE, Bozinovski S, Algar E, Kohmoto T, Ebi H, Ferlin W, Garbers C, Ruwanpura S, Sagi I, Rose-John S, Jenkins BJ (2019) ADAM17 selectively activates the IL-6 trans-signaling/ERK MAPK axis in KRAS-addicted lung cancer. EMBO Mol Med 11:e9976. 158. Sehgal A, Dahlstrom JE, Chan Y, Allison BJ, Miller SL, Polglase GR (2019) Placental histopathology in preterm fetal growth restriction. J Paediatr Child Health 55:582-587. 159. Sehgal A, Gwini SM, Menahem S, Allison BJ, Miller SL, Polglase GR (2019) Preterm growth restriction and bronchopulmonary dysplasia: The vascular hypothesis and related physiology. J Physiol 597:1209-1220. 160. Seow BKL, McDougall ARA, Short KL, Wallace MJ, Hooper SB, Cole TJ (2019) Identification of betamethasone-regulated target genes and cell pathways in fetal rat lung mesenchymal fibroblasts. Endocrinology 160:1868-1884. 161. Severa M, Rizzo F, Srinivasan S, Di Dario M, Giacomini E, Buscarinu MC, Cruciani M, Etna MP, Sandini S, Mechelli R, Farina A, Trivedi P, Hertzog PJ, Salvetti M, Farina C, Coccia EM (2019) A cell type-specific transcriptomic approach to map B cell and monocyte type I interferon-linked pathogenic signatures in Multiple Sclerosis. J Autoimmun 101:1-16. 162. Shao Y, Forster SC, Tsaliki E, Vervier K, Strang A, Simpson N, Kumar N, Stares MD, Rodger A, Brocklehurst P, Field N, Lawley TD (2019) Stunted microbiota and opportunistic pathogen colonization in caesarean-section birth. Nature 574:117-121. 163. Shepherd KL, Yiallourou SR, Odoi A, Brew N, Yeomans E, Willis S, Horne RSC, Wong FY (2019) Effects of prone sleeping on cerebral oxygenation in preterm infants. J Pediatr 204:103-110. 164. Sher I, Daly C, Oehme D, Chandra RV, Sher M, Ghosh P, Smith J, Goldschlager T (2019) Novel application of the Pfirrmann disc degeneration grading system to 9.4T MRI: Higher reliability compared to 3T MRI. Spine 44:E766-E773. 165. Shukla SD, Shastri MD, Chong WC, Dua K, Budden KF, Mahmood MQ, Hansbro NG, Keely S, Eri R, Patel RP, Peterson GM, Hansbro PM (2019) Microbiome-focused asthma management strategies. Curr Opin Pharmacol 46:143-149. 166. Snelgrove SL, Abeynaike LD, Thevalingam S, Deane JA, Hickey MJ (2019) Regulatory T cell transmigration and intravascular migration undergo mechanistically distinct regulation at different phases of the inflammatory response. J Immunol 203:2850-2861. 167. St John JC, Makanji Y, Johnson JL, Tsai TS, Lagondar S, Rodda F, Sun X, Pangestu M, Chen P, Temple-Smith P (2019) The transgenerational effects of oocyte mitochondrial supplementation. Sci Rep 9:6694. 168. Stenning FJ, Hooper SB, Kluckow M, Crossley KJ, Gill AW, Wallace EM, Te Pas AB, LaRosa D, Polglase GR (2019) Transfusion or timing: The role of blood volume in delayed cord clamping during the cardiovascular transition at birth. Front Pediatr 7:405.

169. Stojanovska V, Barton SK, Tolcos M, Gill AW, Kluckow M, Miller SL, Zahra V, Hooper SB, Galinsky R, Polglase GR (2019) The effect of antenatal betamethasone on white matter inflammation and injury in fetal sheep and ventilated preterm lambs. Dev Neurosci 40:497-507. 170. Stringer BW, Day BW, D’Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis PL, Jeffree RL, Johns TG, Boyd AW (2019) A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep 9:4902. 171. Sun T, Ferrero RL, Girardin SE, Gommerman JL, Philpott DJ (2019) NLRC5 deficiency has a moderate impact on immunodominant CD8+ T-cell responses during rotavirus infection of adult mice. Immunol Cell Biol 97:552-562. 172. Szarek M, Bergmann M, Konrad L, Schuppe HC, Kliesch S, Hedger MP, Loveland KL (2019) Activin A target genes are differentially expressed between normal and neoplastic adult human testes: Clues to gonocyte fate choice. Andrology 7:31-41. 173. Tamanyan K, Weichard A, Biggs SN, Davey MJ, Nixon GM, Walter LM, Horne RSC (2019) The impact of central and obstructive respiratory events on cerebral oxygenation in children with sleep disordered breathing. Sleep 42:zsz044. 174. Tedjaseputra A, Vilcassim FS, Grigoriadis G (2019) Ocular infiltration as initial presentation of acute monocytic leukaemia transformed from chronic myelomonocytic leukaemia associated with BRAFV600E mutation. BMJ Case Rep 12:e228519. 175. Teoh SSY, Wang Y, Li Y, Leemaqz SY, Dekker GA, Roberts CT, Nie G (2019) Low serum levels of Htra3 at 15 weeks of gestation are associated with late-onset preeclampsia development and small for gestational age birth. Fetal Diagn & Ther 46:392-401. 176. Thio M, Dawson JA, Crossley KJ, Moss TJ, Roehr CC, Polglase GR, Davis PG, Hooper SB (2019) Delivery of positive end-expiratory pressure to preterm lambs using common resuscitation devices. Arch Dis Child Fetal Neonatal Ed 104:F83-F88. 177. Thomas B, Thillainathan K, Delahunty M, Weichard A, Davey MJ, Nixon GM, Walter LM, Horne RSC (2019) Cardiovascular autonomic control is altered in children born preterm with sleep disordered breathing. J Pediatr 206:83-90. 178. Tran AH, Horne RS, Liew D, Rimmer J, Nixon GM (2019) An epidemiological study of paediatric adenotonsillectomy in Victoria, Australia, 2010-2015: Changing indications and lack of effect of hospital volume on inter-hospital transfers. Clin Otolaryngol 44:1037-1044. 179. Trinh A, Wong P, Fahey MC, Brown J, Strauss BJ, Ebeling PR, Fuller PJ, Milat F (2019) Longitudinal changes in bone density in adolescents and young adults with cerebral palsy: A case for early intervention. Clin Endocrinol (Oxf) 91:517-524.

36 | HUDSON INSTITUTE OF MEDICAL RESEARCH

180. Tseng E, Yee Teoh SS, Wang Y, Nie G (2019) Elevated protease HtrA4 in the maternal circulation of preeclampsia may contribute to endothelial barrier disruption by cleaving key junctional protein VE-cadherin. Placenta 76:51-53. 181. van den Heuij LG, Fraser M, Miller SL, Jenkin G, Wallace EM, Davidson JO, Lear CA, Lim R, Wassink G, Gunn AJ, Bennet L (2019) Delayed intranasal infusion of human amnion epithelial cells improves white matter maturation after asphyxia in preterm fetal sheep. J Cereb Blood Flow Metab 39:223–239. 182. Van Sinderen M, Griffiths M, Menkhorst E, Niven K, Dimitriadis E (2019) Restoration of microRNA-29c in type I endometrioid cancer reduced endometrial cancer cell growth. Oncol Lett 18:2684-2693. 183. Verghese E, Martelotto LG, Cain JE, Williams TM, Wise AF, Hill PA, Langham RG, Watkins DN, Ricardo SD, Deane JA (2019) Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury. BMC Res Notes 12:718. 184. Walter LM, Tamanyan K, Nisbet L, Weichard AJ, Davey MJ, Nixon GM, Horne RSC (2019) Pollen levels on the day of polysomnography influence sleep disordered breathing severity in children with allergic rhinitis. Sleep Breath 23:651-657. 185. Walter LM, Tamanyan K, Weichard AJ, Biggs SN, Davey MJ, Nixon GM, Horne RSC (2019) Age and autonomic control, but not cerebral oxygenation, are significant determinants of EEG spectral power in children. Sleep 42:zsz118. 186. Walter LM, Tamanyan K, Weichard AJ, Davey MJ, Nixon GM, Horne RS (2019) Sleep disordered breathing in children disrupts the maturation of autonomic control of heart rate and its association with cerebral oxygenation. J Physiol 597:819-830. 187. Walton KL, Chen JL, Arnold Q, Kelly E, La M, Lu L, Lovrecz G, Hagg A, Colgan TD, Qian H, Gregorevic P, Harrison CA (2019) Activin A-induced cachectic wasting is attenuated by systemic delivery of its cognate propeptide in male mice. Endocrinology 160:2417-2426. 188. Wang Y, Kong D, Gao Y, Ying L, Huang Q, Xu P (2019) Chemical characterization and bioactivity of phenolics from Tieguanyin oolong tea. J Food Biochem 43:e12894. 189. Wang Y, La M, Pham T, Lovrecz GO, Nie G (2019) High levels of HtrA4 detected in preeclamptic circulation may disrupt endothelial cell function by cleaving the main VEGFA receptor KDR. Faseb J 33:4655-5808. 190. Wang Y, Lim R, Nie G (2019) HtrA4 may play a major role in inhibiting endothelial repair in pregnancy complication preeclampsia. Sci Rep 9:2728. 191. Wang Z, Li J, Liu JP (2019) Effects of cation charges on the binding of stabilizers with human telomere and TERRA G-quadruplexes. J Biomol Struct Dyn 37:1-14. 192. Welch NG, Mukherjee S, Hossain MA, Praveen P, Werkmeister JA, Wade JD, Bathgate RAD, Winkler DA, Thissen H (2019) Coatings releasing the relaxin peptide analogue b7-33 reduce fibrotic encapsulation. ACS Appl Mater Interfaces 11:45511-45519.

193. White CL, Jayasekara WSN, Picard D, Chen J, Watkins DN, Cain JE, Remke M, Gough DJ (2019) A sexually dimorphic role for STAT3 in sonic hedgehog medulloblastoma. Cancers 11:1702. 194. Wilson AL, Wilson KL, Bilandzic M, Moffitt LR, Makanji M, Gorrell MD, Oehler MK, Rainczuk A, Stephens AN, Plebanski M (2019) Non-invasive fluorescent monitoring of ovarian cancer in an immunocompetent mouse model. Cancers (Basel) 11:E32. 195. Wittert G, Atlantis E, Allan C, Bracken K, Conway A, Daniel M, Gebski V, Grossmann M, Hague W, Handelsman D, Inder W, Jenkins A, Keech A, McLachlan R, Robledo K, Stuckey B, Yeap BB (2019) Testosterone therapy to prevent type 2 diabetes mellitus in at-risk men (T4DM): Design and implementation of a double-blind randomized controlled trial. Diabetes Obes Metab 21:772-780. 196. Wong A, Nejad C, Gantier M, Choy KW, Doery J, Graudins A (2019) MicroRNA from a 12-h versus 20-h acetylcysteine infusion for paracetamol overdose. Hum Exp Toxicol 38:646-654. 197. Wooldridge AL, Clifton VL, Moss TJ, Lu H, Jamali M, Agostino S, Muhlhausler BS, Morrison JL, De Matteo R, Wallace MJ, Bischof RJ, Gatford KL (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: Potential mechanisms for programming asthma and allergy. J Physiol 597:4251-4262. 198. Yang X, Devianti M, Yang YH, Ong YR, Tan KS, Gurung S, Tan J, Zhu D, Lim R, Gargett CE, Deane J (2019) Endometrial mesenchymal stem/stromal cell modulation of T cell proliferation. Reproduction 157:43-52. 199. Yang Y, Xiao M, Song Y, Tang Y, Luo T, Yang S, He W, Cheng Q, Ma L, Zhang Y, He Y, Cao Y, Yang J, Peng B, Hu J,Li Q (2019) H-score of 11β-hydroxylase and aldosterone synthase in the histopathological diagnosis of adrenocortical tumors. Endocrine 65:683-691. 200. Yawno T, Sutherland AE, Pham Y, Castillo-Melendez M, Jenkin G, Miller SL (2019) Fetal growth restriction alters cerebellar development in fetal and neonatal sheep. Front Physiol 10:560. 201. Zebaze R, Osima M, Bui M, Lukic M, Wang X, Ghasem-Zadeh A, Eriksen EF, Vais A, Shore-Lorenti C, Ebeling PR, Seeman E, Bjørnerem Å (2019) Adding marrow adiposity and cortical porosity to femoral neck areal bone mineral density improves the discrimination of women with nonvertebral fractures from controls. J Bone Miner Res 34:14511460. 202. Zhang SR, Nold MF, Tang SC, Bui CB, Nold CA, Arumugam TV, Drummond GR, Sobey CG, Kim HA (2019) IL-37 increases in patients after ischemic stroke and protects from inflammatory brain injury, motor impairment and lung infection in mice. Sci Rep 9:6922. 203. Zingue S, Ntsa DM, Magne Nde CB, Michel T, Ndinteh DT, Clyne C, Njamen D (2019) Lupeol, the major compound of the dichloromethane extract of Millettia macrophylla Benth (Fabaceae), displays estrogenic effects in ovariectomized rats. Phytother Res 33:949-957.

REVIEWS 1. Badurdeen S, Roberts C, Blank D, Miller S, Stojanovska V, Davis P, Hooper S, Polglase G (2019) Haemodynamic instability and brain injury in neonates exposed to hypoxia–ischaemia. Brain Sci 9:49. 2. Basurto D, Russo F, Van der Veeken L, Van der Merwe J, Hooper S, Benachi A, Debie F, Gomez O, Deprest J (2019) Prenatal diagnosis and management of congenital diaphragmatic hernia. Best Pract Res Clin Obstet Gynaecol 58:93-106. 3. Bellofiore N, Evans J (2019) Monkeys, mice and menses: The bloody anomaly of the spiny mouse. J Assist Reprod Genet 36:811-817. 4. Cumming HE, Bourke NM (2019) Type I IFNs in the female reproductive tract: The first line of defense in an ever-changing battleground. J Leukoc Biol 105:353-361. 5. Dannappel MV, Sooraj D, Loh JJ, Firestein R (2019) Molecular and in vivo functions of the CDK8 and CDK19 kinase modules. Front Cell Dev Biol 6:171. 6. Findlay JK, Holland MK, Wong BBM (2019) Reproductive science and the future of the planet. Reproduction 158:R91-R96. 7. Fleiss B, Wong F, Brownfoot F, Shearer IK, Baud O, Walker DW, Gressens P, Tolcos M (2019) Knowledge gaps and emerging research areas in intrauterine growth restriction-associated brain injury. Front Endocrinol (Lausanne) 10:188. 8. Funder JW (2019) Primary aldosteronism: Mutations, mechanisms, prevalence, and public health. Hypertension 74:458-466. 9. Gargett CE, Gurung S, Darzi S, Werkmeister JA, Mukherjee S (2019) Tissue engineering approaches for treating pelvic organ prolapse using a novel source of stem/stromal cells and new materials. Curr Opin Urol 29:450457. 10. Giogha C, Lawlor KE (2019) Sugar fix keeps RIPK3 at bay. Immunity 50:539-541. 11. Gu W, Wang L, Wu Y, Liu JP (2019) Undo the brake of tumour immune tolerance with antibodies, peptides and small molecule compounds targeting PD-1/PD-L1 checkpoint at different locations for acceleration of cytotoxic immunity to cancer cells. Clin Exp Pharmacol Physiol 46:105-115. 12. Horne RS, Wijayaratne P, Nixon GM, Walter LM (2019) Sleep and sleep disordered breathing in children with down syndrome: Effects on behaviour, neurocognition and the cardiovascular system. Sleep Med Rev 44:1-11. 13. Horne RSC (2019) Sudden infant death syndrome: Current perspectives. Intern Med J 49:433-438. 14. Huynh J, Chand A, Gough D, Ernst M (2019) Therapeutically exploiting STAT3 activity in cancer — using tissue repair as a road map. Nat Rev Cancer 19:82-96. 15. Lehours P, Ferrero RL (2019) Review: Helicobacter: Inflammation, immunology, and vaccines. Helicobacter 24:e12644. 16. Liu J, Wang L, Wang Z, Liu JP (2019) Roles of telomere biology in cell senescence, replicative and chronological ageing. Cells 8:54.

ANNUAL REPORT 2019 | 37

17. Lum C, Alamgeer M (2019) Technological and therapeutic advances in advanced small cell lung cancer. Cancers (Basel) 11:1570. 18. Mackay J, McCallum Z, Ambler GR, Vora K, Nixon G, Bergman P, Shields N, Milner K, Kapur N, Crock P, Caudri D, Curran J, Verge C, Seton C, Tai A, Tham E, Musthaffa Y, Lafferty AR, Blecher G, Harper J, Schofield C, Nielsen A, Wilson A, Leonard H, Choong CS, Downs J (2019) Requirements for improving health and well-being of children with Prader-Willi syndrome and their families. J Paediatr Child Health 55:1029-1037. 19. Malhotra A, Allison BJ, Castillo-Melendez M, Jenkin G, Polglase GR, Miller SL (2019) Neonatal morbidities of fetal growth restriction: Pathophysiology and impact. Front Endocrinol (Lausanne) 10:55. 20. Marks ZRC, Campbell N, deWeerd NA, Lim SS, Gearing LJ, Bourke NM, Hertzog PJ (2019) Properties and functions of the novel type I interferon epsilon. Semin Immunol 43:101328. 21. Martherus T, Oberthuer A, Dekker J, Hooper SB, McGillick EV, Kribs A, Te Pas AB (2019) Supporting breathing of preterm infants at birth: A narrative review. Arch Dis Child Fetal Neonatal Ed 104:F102-F107. 22. Mayfosh AJ, Baschuk N, Hulett MD (2019) Leukocyte heparanase: A double-edged sword in tumor progression. Front Oncol 9:331. 23. Moffitt L, Karimnia N, Stephens A, Bilandzic M (2019) Therapeutic targeting of collective invasion in ovarian cancer. Int J Mol Sci 20:E1466. 24. Mukherjee S, Darzi S, Paul K, Werkmeister JA, Gargett CE (2019) Mesenchymal stem cell-based bioengineered constructs: Foreign body response, cross-talk with macrophages and impact of biomaterial design strategies for pelvic floor disorders. Interface Focus 9:20180089.

32. Scrivens A, Reynolds PR, Emery FE, Roberts CT, Polglase GR, Hooper SB, Roehr CC (2019) Use of intraosseous needles in neonates: A systematic review. Neonatology 116:305-314.

EPUB AHEAD OF PRINT 1

Aleksova J, Milat F, Kotowicz MA, Pasco JA, Schultz C, Wong P, Ebeling PR, Elder GJ (2019) Patients with end-stage kidney disease have markedly abnormal cortical hip parameters by dual-energy X-ray absorptiometry. Nephrol Dial Transplant DOI: 10.1093/ ndt/gfz195.

Amberg BJ, DeKoninck PLJ, Kashyap AJ, Skinner SM, Rodgers KA, McGillick EV, Deprest JA, Hooper SB, Crossley KJ, Hodges RJ (2019) Placental gas exchange during amniotic carbon dioxide insufflation in sheep. Ultrasound Obstet Gynecol DOI: 10.1002/ uog.21933.

Andrews CJ, Ellwood D, Middleton PF, Homer CSE, Reinebrant HE, Donnolley N, Boyle FM, Gordon A, Nicholl M, Morris J, Gardener G, Davies-Tuck M, Wallace EM, Flenady VJ (2019) Survey of Australian maternity hospitals to inform development and implementation of a stillbirth prevention ‘bundle of care’. Women Birth DOI: 10.1016/j. wombi.2019.06.001.

Bellofiore N, Ellery SJ, Temple-Smith P, Evans J (2019) Pseudopregnancy and reproductive cycle synchronisation cannot be induced using conventional methods in the spiny mouse (Acomys cahirinus). Reprod Fertil Dev DOI: 10.1071/RD18506.

Brouwer E, Te Pas AB, Polglase GR, McGillick EV, Bohringer S, Crossley KJ, Rodgers K, Blank D, Yamaoka S, Gill AW, Kluckow M, Hooper SB (2019) Effect of spontaneous breathing on umbilical venous blood flow and during delayed cord clamping in preterm lambs. Arch Dis Child Fetal Neonatal Ed DOI: 10.1136/archdischild-2018-316044.

Bui DS, Perret JL, Walters EH, Abramson MJ, Burgess JA, Bui MQ, Bowatte G, Lowe AJ, Russell MA, Alif SM, Thompson BR, Hamilton GS, Giles GG, Thomas PS, Morrison S, Johns DP, Knibbs LD, Zock JP, Marcon A, Garcia-Aymerich J, Erbas B, Jarvis D, Svanes C, Lodge CJ, Dharmage SC (2019) Lifetime risk factors for pre- and post-bronchodilator lung function decline: A population-based study. Ann Am Thorac Soc DOI: 10.1513/AnnalsATS.201904-329OC.

Chan M, Wong TCH, Weichard A, Nixon GM, Walter LM, Horne RSC (2019) Sleep macro-architecture and micro-architecture in children born preterm with sleep disordered breathing. Pediatr Res DOI: 10.1038/s41390019-0453-1.

Chong WC, Cain JE (2019) Lessons learned from the developmental origins of childhood renal cancer. Anat Rec (Hoboken) DOI: 10.1002/ar.24315.

Cox AG, Narula S, Malhotra A, Fernando S, Wallace E, Davies-Tuck M (2019) The influence of maternal ethnicity on neonatal respiratory outcome. Arch Dis Child Fetal Neonatal Ed DOI: 10.1136/archdischild-2018-316418.

33. Sehgal A, Murthi P, Dahlstrom JE (2019) Vascular changes in fetal growth restriction: Clinical relevance and future therapeutics. J Perinatol 39:366-374. 34. Shlomovitz I, Speir M, Gerlic M (2019) Flipping the dogma - phosphatidylserine in non-apoptotic cell death. Cell Commun Signal 17:139. 35. Stringer JM, Western PS (2019) A step toward making human oocytes. Nat Biotechnol 37:24-25. 36. Ullah TR (2019) The role of CXCR4 in multiple myeloma: Cells’ journey from bone marrow to beyond. J Bone Oncol 17:100253. 37. Vemuri R, Sylvia KE, Klein SL, Forster SC, Plebanski M, Eri R, Flanagan KL (2019) The microgenderome revealed: Sex differences in bidirectional interactions between the microbiota, hormones, immunity and disease susceptibility. Semin Immunopathol 41:265275. 38. Venerito M, Link A, Rokkas T, Malfertheiner P (2019) Review: Gastric cancer-clinical aspects. Helicobacter 24:e12643. 39. Vetsch J, Wakefield CE, Techakesari P, Warby M, Ziegler DS, O’Brien TA, Drinkwater C, Neeman N, Tucker K (2019) Healthcare professionals’ attitudes toward cancer precision medicine: A systematic review. Semin Oncol 46:291-303. 40. Wemyss MA, Pearson JS (2019) Host cell death responses to non-typhoidal Salmonella infection. Front Immunol 10:1758. 41. Yabal M, Calleja DJ, Simpson DS, Lawlor KE (2019) Stressing out the mitochondria: Mechanistic insights into NLRP3 inflammasome activation. J Leukocyte Biol 105:377-399.

25. Ramshaw JAM, Werkmeister JA, Glattauer V (2019) Recent progress with recombinant collagens produced in Escherichia coli. Curr Opin Biomed Eng 10:149-155. 26. Ranzil S, Walker DW, Borg AJ, Wallace EM, Ebeling PR, Murthi P (2019) The relationship between the placental serotonin pathway and fetal growth restriction. Biochimie 161:80-87. 27. Russo FM, De Bie F, Hodges R, Flake A, Deprest J (2019) Sildenafil for antenatal treatment of congenital diaphragmatic hernia: From bench to bedside. Curr Pharm Des 25:601-608. 28. Salamonsen L (2019) Women in Reproductive Science: My WOMBan’s life: Understanding human endometrial function. Reproduction 158:F55-F67. 29. Santen R, Simpson E (2019) History of estrogen: Its purification, structure, synthesis, biologic actions, and clinical implications. Endocrinology 160:605-625. 30. Sarma UC, Findlay JK, Hutt KJ (2019) Oocytes from stem cells. Best Pract Res Clin Obstet Gynaecol 55:14-22. 31. Schwab R, Lim R, Goldberg R (2019) Resolving intestinal fibrosis through regenerative medicine. Curr Opin Pharmacol 49:90-94.

10 Daniel LC, van Litsenburg RRL, Rogers VE, Zhou ES, Ellis SJ, Wakefield CE, Stremler R, Walter L, Crabtree VM (2019) A call to action for expanded sleep research in pediatric oncology: a position paper on behalf of the International Psycho-Oncology Society Pediatrics Special Interest Group. Psycho-Oncol DOI: 10.1002/pon.5242.

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11 de Guingand DL, Palmer KR, Bilardi JE, Ellery SJ (2019) Acceptability of dietary or nutritional supplementation in pregnancy (ADONS) - exploring the consumer’s perspective on introducing creatine monohydrate as a pregnancy supplement. Midwifery DOI: 10.1016/j.midw.2019.102599. 12 Dekker J, van Kaam AH, Roehr CC, Flemmer AW, Foglia EE, Hooper SB, Te Pas AB (2019) Stimulating and maintaining spontaneous breathing during transition of preterm infants. Pediatr Res DOI: 10.1038/s41390-019-0468-7. 13 Evans J, Hutchison J, Salamonsen LA, Greening DW (2019) Proteomic insights into endometrial receptivity and embryo-endometrial epithelium interaction for implantation critical determinants of fertility. Proteomics DOI: 10.1002/pmic.201900250. 14 Evans J, Walker KJ, Bilandzic M, Kinnear S, Salamonsen LA (2019) A novel “embryo-endometrial” adhesion model can potentially predict “receptive” or “non-receptive” endometrium. J Assist Reprod Genet DOI: 10.1007/ s10815-019-01629-0. 15 Fernando S, Wallace EM, Rombauts L, White N, Hong J, Vollenhoven B, Lolatgis N, Hope N, Wong M, Lawrence M, Lawrence A, Russell C, Leong K, Thomas P, da Silva Costa F (2019) The effect of melatonin on ultrasound markers of follicular development: A double-blind placebo-controlled randomised trial. Aust N Z J Obstet Gynaecol DOI: 10.1111/ ajo.13074. 16 Funder J (2019) Primary aldosteronism: Treatment of the disease, and new therapeutic approaches. Best Pract Res Clin Endocrinol Metab DOI: 10.1016/j.beem.2019.101368. 17 Harman K, Weichard AJ, Davey MJ, Horne RSC, Nixon GM, Edwards BA (2019) Assessing ventilatory control stability in children with and without an elevated central apnoea index. Respirology DOI: 10.1111/resp.13606. 18 Hobson SR, Wallace EM, Chan YF, Edwards AG, Teoh MWT, Khaw AP (2019) Mirroring preeclampsia: The molecular basis of Ballantyne syndrome. J Matern Fetal Neonatal Med DOI: 10.1080/14767058.2018.1500550. 19 Horne RSC, Ong C, Weichard A, Nixon GM, Davey MJ (2019) Are there gender differences in the severity and consequences of sleep disordered in children? Sleep Med DOI: 10.1016/j.sleep.2019.11.1249. 20 Juonala M, Lewis S, McLachlan R, Hammarberg K, Kennedy J, Saffery R, McBain J, Welsh L, Cheung M, Doyle LW, Amor DJ, Burgner DP, Halliday J (2019) American Heart Association ideal cardiovascular health score and subclinical atherosclerosis in 22-35-yearold adults conceived with and without assisted reproductive technologies. Hum Reprod DOI: 10.1093/humrep/dez240. 21 Kashyap AJ, Hodges RJ, Thio M, Rodgers KA, Amberg BJ, McGillick EV, Hooper SB, Crossley KJ, DeKoninck PLJ (2019) Physiologically based cord clamping improves cardiopulmonary haemodynamics in lambs with a diaphragmatic hernia. Arch Dis Child Fetal Neonatal Ed DOI: 10.1136/archdischild-2019-316906

22 Kilchert C, Strasser K, Kunetsky V, Anko ML (2019) From parts lists to functional significance-RNA-protein interactions in gene regulation. Wiley Interdiscip Rev RNA DOI: 10.1002/wrna.1582. 23 King A, Blank D, Bhatia R, Marzbanrad F, Malhotra A (2019) Tools to assess lung aeration in neonates with respiratory distress syndrome. Acta Paediatr DOI: 10.1111/ apa.15028. 24 Knol R, Brouwer E, van den Akker T, DeKoninck P, van Geloven N, Polglase GR, Lopriore E, Herkert E, Reiss IKM, Hooper SB, Te Pas AB (2019) Physiological-based cord clamping in very preterm infants - Randomised controlled trial on effectiveness of stabilisation. Resuscitation DOI: 10.1016/j.resuscitation.2019.12.007. 25 Lalaoui N, Boyden SE, Oda H, Wood GM, Stone DL, Chau D, Liu L, Stoffels M, Kratina T, Lawlor KE, Zaal KJM, Hoffmann PM, Etemadi N, Shield-Artin K, Biben C, Tsai WL, Blake MD, Kuehn HS, Yang D, Anderton H, Silke N, Wachsmuth L, Zheng L, Moura NS, Beck DB, Gutierrez-Cruz G, Ombrello AK, Pinto-Patarroyo GP, Kueh AJ, Herold MJ, Hall C, Wang H, Chae JJ, Dmitrieva NI, McKenzie M, Light A, Barham BK, Jones A, Romeo TM, Zhou Q, Aksentijevich I, Mullikin JC, Gross AJ, Shum AK, Hawkins ED, Masters SL, Lenardo MJ, Boehm M, Rosenzweig SD, Pasparakis M, Voss AK, Gadina M, Kastner DL, Silke J (2019) Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease. Nature DOI: 10.1038/s41586-0191828-5. 26 Lin C, Yang J, Fuller PJ, Jing H, Song Y, He W, Du Z, Luo T, Cheng Q, Yang S, Wang H, Li Q, Hu J (2019) A combination of captopril challenge test after saline infusion test improves diagnostic accuracy for primary aldosteronism. Clin Endocrinol (Oxf) DOI: 10.1111/cen.14134. 27 Liu X, Mao Y, Kang Y, He L, Zhu B, Zhang W, Lu Y, Wu Q, Xu D, Shi L (2019) MicroRNA-127 promotes anti-microbial host defense through restricting A20-mediated de-ubiquitination of STAT3. iScience DOI: 10.1016/j. isci.2019.100763. 28 Malhotra A, Lim R, Mockler JC, Wallace EM (2019) Two-year outcomes of infants enrolled in the first-in-human study of amnion cells for bronchopulmonary dysplasia. Stem Cells Transl Med DOI: 10.1002/sctm.19-0251. 29 Mank A, Carrasco Carrasco C, Thio M, Clotet J, Pauws SC, DeKoninck P, Te Pas AB (2019) Tidal volumes at birth as predictor for adverse outcome in congenital diaphragmatic hernia. Arch Dis Child Fetal Neonatal Ed DOI: 10.1136/archdischild-2018-316504. 30 Martherus T, den Hoed A, Cramer SJE, Tan R, Hooper SB, Te Pas AB (2019) Paediatric exhaled CO2 detector causes leaks. Arch Dis Child Fetal Neonatal Ed DOI: 10.1136/archdischild-2019-317729. 31 McDonald CA (2019) Impact of mesenchymal stromal cell delivery through cardiopulmonary bypass on postnatal neurogenesis (Commentary). Ann Thorac Surg DOI: 10.1016/j. athoracsur.2019.09.032.

32 Murthi P, Vaillancourt C (2019) Placental serotonin systems in pregnancy metabolic complications associated with maternal obesity and gestational diabetes mellitus. Biochim Biophys Acta Mol Basis Dis DOI: 10.1016/j. bbadis.2019.01.017. 33 Ng IHX, da Costa CS, Zeiler FA, Wong FY, Smielewski P, Czosnyka M, Austin T (2019) Burden of hypoxia and intraventricular haemorrhage in extremely preterm infants. Arch Dis Child Fetal Neonatal Ed DOI: 10.1136/archdischild-2019-316883. 34 Piessens S, Edwards A (2019) Sonographic evaluation for endometriosis in routine pelvic ultrasound. J Minim Invasive Gynecol DOI: 10.1016/j.jmig.2019.08.027. 35 Rudloff I, Jarde T, Bachmann M, Elgass KD, Kerr G, Engel R, Richards E, Oliva K, Wilkins S, McMurrick PJ, Abud HE, Mühl H, Nold MF (2019) Molecular signature of interleukin-22 in colon carcinoma cells and organoid models. Transl Res DOI: 10.1016/j.trsl.2019.10.004. 36 Saad MI, McLeod L, Yu L, Ebi H, Ruwanpura S, Sagi I, Rose-John S, Jenkins BJ (2019) The ADAM17 protease promotes tobacco smoke carcinogen-induced lung tumourigenesis. Carcinogenesis DOI: 10.1093/carcin/ bgz123. 37 Shepherd KL, Yiallourou SR, Odoi A, Yeomans E, Willis S, Horne RSC, Wong FY (2019) When does prone sleeping improve cardiorespiratory status in preterm infants in the NICU? Sleep DOI: 10.1093/sleep/zsz256. 38 Signorelli C, Wakefield C, McLoone JK, Fardell J, Jones JM, Turpin KH, Emery J, Michel G, Downie P, Skeen JE, Cohn R (2019) Childhood cancer survivorship: Barriers and preferences. BMJ Support Palliat Care DOI: 10.1136/bmjspcare-2019-002001. 39 Speir M, Nowell CJ, Chen AA, O’Donnell JA, Shamie IS, Lakin PR, D’Cruz AA, Braun RO, Babon JJ, Lewis RS, Bliss-Moreau M, Shlomovitz I, Wang S, Cengia LH, Stoica AI, Hakem R, Kelliher MA, O’Reilly LA, Patsiouras H, Lawlor KE, Weller E, Lewis NE, Roberts AW, Gerlic M, Croker BA (2019) Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation. Nat Immunol DOI: 10.1038/s41590-019-0550-7. 40 Sutherland K, Weichard AJ, Davey MJ, Horne RS, Cistulli PA, Nixon GM (2019) Craniofacial photography and association with sleep-disordered breathing severity in children. Sleep Breath DOI: 10.1007/s11325-019-01928-x. 41 Takamura M, Zhou W, Rombauts L, Dimitriadis E (2019) The long non-coding RNA PTENP1 regulates human endometrial epithelial adhesive capacity in vitro: Implications in infertility. Biol Reprod DOI: 10.1093/biolre/ ioz173. 42 Thiengtavor C, Siriworadetkun S, Paiboonsukwong K, Fucharoen S, Pattanapanyasat K, Vadolas J, Svasti S, Chaichompoo P (2019) Increased ferritin levels in non-transfusion-dependent β0-thalassaemia/HbE are associated with reduced CXCR2 expression and neutrophil migration. Br J Haematol DOI: 10.1111/bjh.16295.

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43 Walter LM, Shepherd KL, Yee A, Horne RSC (2019) Insights into the effects of sleep disordered breathing on the brain in infants and children: Imaging and cerebral oxygenation measurements. Sleep Med Rev DOI: 10.1016/j.smrv.2019.101251. 44 Wang Z, Chen Z, Li J, Huang J, Zheng C, Liu JP (2019) Combined 3D-QSAR, molecular docking and molecular dynamics study on the benzimidazole inhibitors targeting HCV NS5B polymerase. J Biomol Struct Dyn DOI: 10.1080/07391102.2019.1593244. 45 Wilkie B, Buckle A, Allan C, Richardson M, Keong B (2019) Acute superior mesenteric vein thrombosis with ischaemic bowel in Klinefelter syndrome. ANZ J Surg DOI: 10.1111/ans.15507. 46 Yang C, McDermot DS, Pasricha S, Bedoui S, Lenz LL, van Driel IR, Hartland EL (2019) IFNγ receptor down-regulation facilitates Legionella survival in alveolar macrophages. J Leukoc Biol DOI: 10.1002/jlb.4ma1019-152r. 47 Yang J, Fuller PJ (2019) Simplifying the diagnosis of primary aldosteronism. J Clin Endocrinol Metab DOI: 10.1210/clinem/dgz202. 48 Zhang W, Zhuang N, Liu X, He L, He Y, Mahinthichaichan P, Zhang H, Kang Y, Lu Y, Wu Q, Xu D, Shi L (2019) The metabolic regulator Lamtor5 suppresses inflammatory signaling via regulating mTOR-mediated TLR4 degradation. Cell Mol Immunol DOI: 10.1038/ s41423-019-0281-6.

40 | HUDSON INSTITUTE OF MEDICAL RESEARCH

Graduates of 2019

Congratulations to our Postgraduate and Honours students who graduated in 2019

DOCTOR OF PHILOSOPHY

Dr Douglas Blank

Dr Paris Papagianis

Dr Aminath (Azu) Azhan

The effects of umbilical cord milking at birth

Human amnion epithelial stem cells are a treatment to chronic inflammation

Prof Stuart Hooper AM, A/Prof Graeme Polglase

A/Prof Tim Moss, A/Prof Graeme Polglase, A/Prof Jane Pillow

Dr James Ong

Dr Paulo Pinares-Garcia

Optimising inflammasome inhibitors to treat pandemic influenza

The role of the male gene SRY in healthy and injured nigrostriatal dopamine neurons

Impaired myelination in the growth restricted brain: Upregulation of Wnt and Notch signalling pathways in delayed oligodendrocyte maturation A/Prof Mary Tolcos, A/Prof Flora Wong, Prof David Walker Dr Atul Malhotra

A/Prof Ashley Mansell, Dr Michelle Tate

Novel therapies for intrauterine growth restriction related brain injury

Dr Jasna Aleksova

A/Prof Suzanne Miller, Prof Graham Jenkin, Dr Margie Zakhem

The effect of androgen replacement therapy on bone and muscle health in men with chronic kidney disease

Dr Benjamin Nowotny Using obstetric complaints and litigation as a quality improvement tool Prof Euan Wallace AM, Dr Miranda Davies-Tuck, Prof Erwin Loh Dr Catherine Cochrane Investigating the role of Sonic hedgehog signalling in cerebellar development and disease

Dr Joohyung Lee, Prof Vincent Harley

A/Prof Frances Milat, Prof Peter Ebeling AO, Prof Grahame Elder, Prof Rob McLachlan Dr Ke Tang STAT3-dependent regulation of inflammasomes promotes gastric tumourigenesis through regulating interleukin 1 production in glycoprotein 130 mutant mice

Dr Rashid Abdulhameed Aldahhan The genetic causes of male infertility Prof Mark Hedger, Prof David de Kretser AC, Prof Peter Stanton Dr Sebastian Hobson A pilot study of antenatal maternally administered melatonin to decrease the level of oxidative stress in human pregnancies affected by preeclampsia (PAMPR Trial) Prof Euan Wallace AM, A/Prof Rebecca Lim

Dr Jason Cain, Prof Neil Watkins

Prof Brendan Jenkins, Dr Daniel Croagh

Dr Chun Wang (Jason) Lao

Dr Maciej Szarek

Exploring a new frontier: The immune and coagulation systems of the premature infant and their relevance in major diseases of prematurity

Determining gonocyte migration

Use of minocycline and related drugs to suppress microglial activation after hypoxia and infection

Prof Kate Loveland, Prof Mark Hedger, Prof Martin Bergmann

Prof Caroline Gargett, Prof David Walker

Prof Marcel Nold, A/Prof Claudia Nold, Dr Ina Rudloff, Dr Niamh Mangan

Dr Mohamed Ibrahim Ahmed Saad

Dr Shenpeng Zhang

Amnion cells - a potential cure for idiopathic pulmonary fibrosis. Uncovering the underlying molecular mechanism

Effects of T helper 2-promoting agents on outcome after stroke

Dr Dana Pueschl Immune privilege and neoplasia in human testis: potential role and functional polarization of macrophages and dendritic cells Prof Martin Bergmann, Prof Kate Loveland, Prof Mark Hedger, Prof Tina Treczek

Prof Brendan Jenkins, Dr Saleela Ruwanpura

Dr Sebastian Quezada

Prof Chris Sobey, Dr Hyun Ah Kim, Prof Marcel Nold Dr Shreya Rana

Dr Mohammed Ibrahim A Alasseiri

Investigation into the mechanisms underlying cortical folding

Prevention of myelodysplastic syndrome progression to acute myeloid leukaemia by iron modulation

A/Prof Tim Moss, Prof David Walker, A/ Prof Mary Tolcos

Prof Bryan Williams, Dr Afsar Ahmed

ANNUAL REPORT 2019 | 41

Dr Stuart Emmerson Elevation of tissue engineering construction comprising autologous endometrial mesenchymal stem cells

BACHELOR OF MEDICAL SCIENCE (HONOURS) Mr Alexander Bell Ms Daisy Lu

Prof Caroline Gargett, Prof Jerome Werkmeister

Ms Shalini Pannampalam

Dr Sultan Mohammed Alhayyani

Mr Ravindu Sellahewa

The mechanistic role of glycoprotein 130 signalling in lung cancer development

Mr Andrew Stainsby

Prof Brendan Jenkins, Prof Philip Bardin

Ms Evelyn Turek

Dr Yao Wang HtrA serine peptidase 4-induced endothelial dysfunction in early-onset preeclampsia Prof Guiying Nie, A/Prof Craig Harrison BACHELOR OF BIOMEDICAL SCIENCE (HONOURS) Ms Paige Anderson Ms Rheannon Blucher Ms Emilie Guy Ms Lauren Hadley Ms Rona Lepatan Ms Hannah McDonald Ms Lisa-Jane Oldfield Ms Pranjal Patel Ms Olivia Payne Ms Bothidah Thach Ms Chamali Wadanambi Ms Nishadi Withanage Ms Katherine Wyatt

Ms Emily Pryor

Ms Rebecca Stone

BACHELOR OF SCIENCE (HONOURS) Mr Lohith Appaneravanda Ms Aida Azlan Ms Courtney Barber Ms Sarah Chellew Ms Sharmony Kelly Mr Jia Jian (JJ) Loh Mr Michael Luu Ms Maria Petraki Ms Amelia Matthews Ms Madeline Nicola Ms Greta Paterson Ms Enola Roussel Ms Ashwini Sakthiakumara Ms Remy Young

27-31 Wright Street Clayton VIC 3168 Australia

t: +61 3 8572 2700 e: info@hudson.org.au w: hudson.org.au

Annual Report 2019

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2019 Publications

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Director’s report

Inflammation

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