Tamar Didbaridze

This study provides a contemporary epidemiology of aspirates taken during surgery from the abdominal cavity among patients with bacterial peritonitis to identify the isolates and study their sensitivity to antibiotics. Our bacteriology investigations included isolation of poor cultures, and detection of microbes was conducted using a rapid identification system (API20E, API Staph, API Strep, API Ana, BioMerieux). Rapid tests for detection of oxidase and catalase activity were also used. Susceptibility of microorganisms to antibiotics was defined by the disc-diffusion method using standard discs (EUCAST guidelines 2015) according to Clinical Laboratory Standard Institute (CLSI) protocols (ATB strips: ATB G, ATB Staph, ATBANA, ATBPse, ATBStrep. BioMerieux). The recovery rate from the clinical samples was good, likely because our protocol immediately inoculated study material into the thioglycollate broth which is an appropriate medium both for aerobic and anaerobic bacteria. Among the...

Objective This study aims to determine the relationship between the presence of urinary nitrite and bacterial resistance to antimicrobial therapy in patients with uncomplicated urinary tract infections. Methods During a six-month time period (April-October, 2020), we reviewed the urine samples of 59 adult outpatients from the Urology Department of Tbilisi State Medical University the First University Clinic with the diagnosis of urinary tract infection. The infecting microorganisms and the presence of urine nitrite were recorded. Resistance rates to the antibiotics were compared between the positive and negative nitrite groups. Chi-squared test was used to perform the statistical analysis using Prism software version 9.3.1 (GraphPad Software, Inc., San Diego, California). Results We examined the correlation between the nitrite-positive and -negative groups with the resistance pattern to ceftriaxone, trimethoprim/sulfamethoxazole (TMP-SMX), ampicillin-sulbactam, fosfomycin, amikacin, doxycycline, cefuroxime, cefotaxime, ceftazidime, and nitrofurantoin. A total of 59 outpatients with a mean age of 37 years met the inclusion criteria between April and October 2020. In the positive and negative nitrite groups, there were 23 and 36 patients, respectively. Three (17.6%) of the 17 gram-positive organisms and 20 (62.5%) of the 42 gram-negative organisms yielded positive nitrite results. In nitrite-positive group, resistance rates to ceftriaxone, TMP-SMX, ampicillin-sulbactam, fosfomycin, amikacin, doxycycline, cefuroxime, cefotaxime, ceftazidime, and nitrofurantoin were 52.2%, 70.8%, 63.5%, 67.7%, 25.8%, 31.9%, 29.6%, 32.5%, 22.5% and 83.8%, respectively. These values in the nitrite-negative group were 6.5%, 41.3%, 60.7%, 72.9%, 49%, 3%, 2.3%, 3.3%, 4.3% and 81.9%, respectively. Highest relative resistance rate was recorded against cefuroxime (12.9), followed by doxycycline (10.6), cefotaxime (9.8), ceftriaxone (8.03), ceftazidime (5.2), TMP-SMX (1.71), ampicillin-sulbactam (1.05), nitrofurantoin (1.02), fosfomycin (0.93), and amikacin (0.53). The most commonly isolated pathogen was Escherichia coli, which was detected in 35 (71%) isolates. Other bacteria commonly found were Proteus spp in five (12%) isolates, Klebsiella spp in two (5%) isolates, and Enterococcus in five (12%) isolates. Conclusion The findings revealed that out of 10 antibiotics, nitrite-positive groups demonstrated higher resistance only against ceftriaxone, cefuroxime, cefotaxime, and doxycycline. Other antibiotics showed no statistically significant differences in resistance. Furthermore, the highest relative resistance rate was recorded against cefuroxime, whereas amikacin revealed the lowest. Therefore, we suggest physicians to not adjust antibiotic therapy for urinary tract infections (UTIs) based on the presence of nitrite. Urine bacteriology should be ordered.

The First International Scientific Practical Online Conference Human Genetics and Genetic Diseases: Problems and Development Perspectives <br> Date: 10.00 A.M., 30 - 31. 05. 2020<br> Venue: Azerbaijan Medical University, Baku, Azerbaijan. Address: 163, Samad Vurgun Street, Building 5.

A structured and algorithmic model of the control system, which allows multi-level complex monitoring on the basis of measuring channels controlled by variable range for remote detection of leakage sites in the main pipelines, has been developed. The air-gas composition change in the leak site of the pipeline leads to changes in the absorption and radiation intensity due to its impact on the radiation obtained from that site. The used liquid crystal filters have a wider range of bandwidth control and is polarized-independent providing to obtain the results of a two-color measurement. The values of measurement results obtained from the infrared range in the monitoring system represent the current leakage state and obtaining the appropriate data in the visible range allow the confirmation of the obtained data in the infrared range and thereby increase the reliability of the received information about the leakage. Decoding infrared data in the visible range makes it possible to visuali...

Anaerobic clostridial infection is the most severe form of paraproctitis. The incubation period is very short, from 3 to 6 hours, sometimes lasting for 1-2 days. Clostridial infection spreads rapidly and induces gas gangrene, causes destruction of cells and other intermediate substances, and impedes blood circulation. This paper presents a case study of an extremely severe form of anaerobic infection with spontaneous gas gangrene, cellulitis, fasciomyositic necrosis, severe intoxication and septic shock on the abdominal front and lateral surfaces. This patient presented as infected with Clostridium septicum, a rare and highly toxic Gram-positive, spore-forming, obligate anaerobic bacillus that progresses and migrates rapidly, affecting all soft tissues (muscle, fascia), and produces four toxins which cause gas gangrene, intravascular hemolysis, tissue necrosis, and septic shock. The mortality rate is typically 80%. In this case study, a positive clinical outcome was achieved by aggr...

Didbaridze T. 1 , Kochiashvili D. 2 , Kvakhajelidze V. 3 , Kochiashvili G. 3 and Gogokhia N 5 . 1. Microbiologist MD. PhD. TSMU The First University Clinic (Tbilisi, Georgia). 2. Head of Department of Urology, TSMU Professor. MD. PhD (Tbilisi, Georgia). 3. Urologist. TSMU Department of Urology (Tbilisi, Georgia). 4. TSMU Department of Urology. Assistant professor. MD. PhD(Tbilisi, Georgia). 5. Head of Clinical laboratory MD. PhD. TSMU the First University Clinic (Tbilisi, Georgia). ...................................................................................................................... Manuscript Info Abstract ......................... ........................................................................ Manuscript History

Gastrointestinal cancers (GI) are one of the most abundant types of cancers among the world population, though statistical data indicate that in eastern Asia these types of cancer occur 4 times more often than in Western Europe. Absence of treatment of bacterial infections, obesity, and lack of vegetable food in a diet can be the case of GI cancer. All pathologies are inevitably connected to the changes in cell cycle, abnormal protein amount and their dysfunction. Serum proteins are widely used as an additional source of information about body condition, also changes in protein composition can point out the mechanism of disease development and effectiveness of treatment. In the presented work we studied protein composition of GI cancer patients in different stages of cancer development, after and before chemotherapy and compared these data to protein composition of healthy control group of voluntaries. Treatment of patients was performed according the guidelines appropriate for the ...

Perinephric abscess is an uncommon complication of urinary tract infections. The incidence ranges from 1-10 cases for every 10 000 hospital admissions. Men and women are affected with equal frequency.E.coli, Proteus speacies and Staphylococcus aureus are the unusual etiologic organisms. There were very few reports about urinary tract infections such as renal perinephric abscess complicated with psoas muscle abscess. Renal and perirenal abscess can complicate a urologic infection(usually due to gram-negative enteric bacilli or a polymicrobial infection). This clinical case of a 24 year old men with predisposing condition for secondary psoas muscle abscess(6 year history of the right non functional kidney), is interesting because of unusual etiologic organisms for psoas muscle abscess Proteus mirabilis,which is highly flagellate , have stone formation ability which is very difficult to clear with only antibiotics, has predilection for the kidney and are difficult to eradicate.

The precise role of bacterial infection in the course of exacerbation COPD has been a source of controversy for decades.Chronic bacterial colonization of the lower airways contributes to airway inflammation; The course of COPD is characterized by intermittent exacerbacions of the disease.Approximately 40-50% acute exarbations are caused by bacteria, by viruses in 25%, and both viruses and bacteria in another 25%. Airway inflammation is increased during the exacerbation resulting in increased hyperinflation, reduced expiratory air flow and decreased gas exchange. Expectorated sputum is the most commonly used sample for diagnosis of lower respiratory tract infections(LRTI),which can be obtained easily and non-invasively. The bacteria are very common in the lower respiratory tract of patients with COPD and cause inflammation. We have shown that among microorganisms causing acute exacerbations of COPD in our patients prevailed gram negative Enterobacteriaceae family and comparatively hi...

The influence of Gram-positive uropathogenic bacteria on sperm morphology and function has been poorly investigated until now.The effects of bacterial presence on sperm quality and fertility are controversial. S. aureus is one of the dominant bacteria isolated from the semen samples of males complaining of infertility. Bacteria are capable of agglutinating and immobilizing spermatozoa. S. aureus reduced sperm motility. Although S. aureus has been reported to cause immobilization of spermatozoa, however, its role in infertility has yet to be elucidated. We retrospectively have studied the medical records of the 28 patients who visited TSMU The First University Clinic Department of Urology from 2017 January until May 2017 with complaining of infertility and who had bacteriologicaly Staphylococcus aureus in seminal fluid, no other urophatogens(Ureaplasma, Chlamidia, Mycoplasma) and risk factors for infertility were found and determined the influence of Staphylococcus aureus on semen qu...

Yeast species belonging to the Candida genus, including Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, and Candida krusei, are the most prevalent fungal species inhabiting various mucosal surfaces, such as the skin and the respiratory, digestive, and urinary tracts [1]. Although being commensal within the human host, Candida species are equipped with virulence attributes, enabling them to invade when opportunities arise and cause various infections in humans, especially when the immune system is impaired.  We retrospectively studied total lymphocytes count and   Candida infection in 142 patients   who were admitted to TSMU The First university Clinic Intensive care unit department between 14.10.20 – 02.02.21 . Among  142 patients Candida  spp  were isolated in 15,4%(22/142) patients. In all this patients  total lymphocytes count were low.  Candidemia was  not dectected.  Candiduria was observed in 2 patients(9%, 2/22). Our data is close to the  White, ...

Nocardiosis is a vital, but often ignored, infectious disease in immunocompromised hosts, which is particularly serious in the absence of timely diagnosis and therapy. Nocardiosis affects patients who display a cellular immunodeficiency, such as transplant recipients on immunosuppressive treatment, but uncommonly associated with high morbidity and mortality rates. Disseminated Nocardiosis affecting the central nervous system (CNS), abdomen, skin and lungs .Nocardia spp. infection can be observed and confirmed by subculture and positive microscopic detection of a branching gram-positive rod. Infection commonly enters via the respiratory route but may also occur through skin trauma. A case of disseminated nocardiosis in an immunocompetent individual after car accident is presented. The case highlights challenges surrounding diagnosis and microbiological identification of Gram-positive branching bacilli, patient management, and choice of antibiotic.