Raw data from manuscript.
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The origins of low-temperature tissue storage research date back to the late 1800s. Over half a century later, osmotic stress was revealed to be a main contributor to cell death during cryopreservation. Consequently, the addition of... more
The origins of low-temperature tissue storage research date back to the late 1800s. Over half a century later, osmotic stress was revealed to be a main contributor to cell death during cryopreservation. Consequently, the addition of cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol (GLY), ethylene glycol (EG), or propylene glycol (PG), although toxic to cells at high concentrations, was identified as a necessary step to protect against rampant cell death during cryopreservation. In addition to osmotic stress, cooling and thawing rates were also shown to have significant influence on cell survival during low temperature storage. In general, successful low-temperature cell preservation consists of the addition of a CPA (commonly 10% DMSO), alone or in combination with additional permeating or non-permeating agents, cooling rates of approximately 1ºC/min, and storage in either liquid or vapor phase nitrogen. In addition to general considerations, cell-specific re...
Research Interests:
Research Interests: Locomotion, Medicine, Multidisciplinary, Anxiety, Learning, and 15 moreReward, Humans, Behaviour, Animal, Mice, Nicotine, Female, Animals, Male, Gender Differences, PLoS one, Disease models, Conditioning, ANXIETY, and Cannabinoid(Reward, Humans, Behaviour, Animal, Mice, Nicotine, Female, Animals, Male, Gender Differences, PLoS one, Disease models, Conditioning, ANXIETY, and Cannabinoid)
(Reward, Humans, Behaviour, Animal, Mice, Nicotine, Female, Animals, Male, Gender Differences, PLoS one, Disease models, Conditioning, ANXIETY, and Cannabinoid)
The global system is moving away from an order in which a Western state, most recently the US, is at the centre. Instead, the rise of China and India and other emerging economies, has caused a shift towards multipolarity. There is cause... more
The global system is moving away from an order in which a Western state, most recently the US, is at the centre. Instead, the rise of China and India and other emerging economies, has caused a shift towards multipolarity. There is cause for concern as these new powers are not founded on the same values as those of Europe and the US. The decisions made are thus not only unaligned with the West, but these emerging economies also possess the power to implement and reinforce their decisions. Europe has taken certain initiatives to maintain some semblance of influence on today’s global stage; however, these initiatives are not flawless.