Best Mode for Carrying Out the Invention
The present invention relates to a preservative composition comprising Sodium Metabisulfite (SDS) and Cetylpyridinium Chloride (CPC).
The preservative composition has a sterilizing effect or a germicidal effect on a microorganism and includes both an antibacterial effect on bacteria and an antifungal effect on fungi.
The sodium metabisulfite (SDS) was used as an antioxidant and a reducing agent, and it was confirmed that the composition of the present invention was excellent in antibacterial and antifungal effect by mixing with CPC. In particular, although SDS has a disadvantage in that sulfuric acid is caught at a pH of less than 5.0, the preservative composition of the present invention has an effect of improving physical stability and odor.
The preservative composition may contain SDS and CPC in a weight ratio of 1: 0.2 to 5, preferably 1: 0.2 to 0.25 or 1: 4 to 5. When the weight ratio is satisfied, it is preferable to stabilize physical properties and to improve antimicrobial and antifungal properties of the preservative composition.
The preservative composition may comprise 0.001 to 20% by weight of sodium metabisulfite, 0.001 to 20% by weight of cetylpyridinium chloride and a balance of purified water.
If the amount of cetylpyridinium chloride is less than 0.001% by weight based on the total weight of the preservative composition, the antimicrobial and antifungal activity against the target microorganism is poor and the broad spectrum antimicrobial spectrum is not obtained. If the concentration exceeds 20% by weight, the skin toxicity and physiological function And it is difficult to control physical properties such as low-temperature stability, high-temperature stability and pH stability at the time of production, and is incompatible with other compounds because of low compatibility with other compounds.
If the amount of the sodium metabisulfite is less than 0.001% by weight based on the total weight of the preservative composition, the antimicrobial and antifungal activity against the target microorganism is poor and does not have a broad spectrum of antimicrobial activity. If the content exceeds 20% by weight, And it is difficult to control physical properties such as low-temperature stability, high-temperature stability and pH stability at the time of production, and it is not suitable for use since it is not compatible with other compounds.
The preservative composition may further include any one selected from the group consisting of sodium sulfite, hexylene glycol, a surfactant, and combinations thereof. When the above components are further included, the mixing ratio of SDS and CPC is improved, antibacterial and antifungal activity of the preservative composition can be further improved, freezing point and recrystallization temperature are lowered, pH stability is stabilized, It has excellent effect.
In the case of the sodium sulfite, SDS and sodium sulfite may be contained in a weight ratio of 1: 0.1 to 1.5, preferably 0.5 to 1.5. If the content of sodium sulfite is less than 0.1 weight ratio, it is difficult to control the freezing point and recrystallization temperature of the preservative composition, which is a problem in the stabilization of the product. When the content is more than 1.5 weight%, it is difficult to control the physical properties such as low temperature stability and high temperature stability, There is a problem with.
In the case of hexylene glycol, SDS and hexylene glycol may be contained in a weight ratio of 1: 0.1 to 1.5, preferably 0.5 to 1.5. When the content of sodium sulfite is less than 0.1 weight ratio, it is difficult to control the freezing point and recrystallization temperature of the preservative composition, which is a problem in stabilizing the product. When the sodium sulfite content is more than 1.5 weight ratio, it is difficult to control the physical properties such as low temperature stability and high temperature stability, There is a problem with stabilization.
In the case of the above surfactant, the weight ratio of SDS to surfactant may be 1: 0.1 to 300, preferably 1: 0.1 to 10. When the content of the surfactant is out of the above range, it is difficult to control the freezing point and the recrystallization temperature of the preservative composition, and it is difficult to stabilize the pH, thereby causing a problem of stabilization of the product.
The surfactant may be anionic, non-ionic or amphoteric. The anionic surfactant may be in the form of a carboxylate salt, a sulfuric acid ester salt, a sulfonate salt, or a phosphate ester salt, and may be in the form of a sodium salt, a calcium salt, an ammonium salt or an ethanolamine salt. Non- Polyethylene glycol type or polyhydric alcohol type, and the amphoteric surfactant may be an amino acid type or betaine type.
More specifically, examples of the anionic surfactant include fatty acid salts, alkyl dicarboxylic acid salts, alcohol sulfate ester salts, higher alkyl sulfonic acid salts, higher alkyl disulfonic acid salts, sulfated fatty acids and fatty acid sulfonated higher fatty acid salts, Ester salts, and the like.
The amphoteric surfactants include betaine type amphoteric surfactants, glycine type amphoteric surfactants, alanine type amphoteric surfactants, and sulfobetaine amphoteric surfactants.
The non-ionic surfactants include glycerin esters of higher fatty acids, glycol esters of higher fatty acids, pentaerythritol esters of higher fatty acids, sucrose esters of higher fatty acids, sorbitan of higher fatty acids and higher alcohol condensates of mannitansters, Higher fatty acid amide condensates, higher alkylamine condensates, higher alkylmercaptan condensates, alkylphenol condensates, polypropylene oxide condensates, and mixtures thereof, but may be any of those selected from the group consisting of .
The preservative composition can be one wherein the formulation is selected from aqueous solutions, emulsions, dispersions, suspensions and oil solutions.
The preservative composition may further contain additives such as a flavor, a vegetable extract, a pH adjuster, other additives, purified water, a thickener, and an antioxidant.
The preservative composition of the present invention can be prepared by mixing sodium metabisulfite (SDS) and cetylpyridinium chloride (CPC) and optionally sodium sulfite, hexylene glycoll, A surfactant, a surfactant, an additive and a combination thereof may be mixed at room temperature, preferably at 25 ° C or higher and 80 ° C or lower. Preferably, the mixture is prepared by ultrasonic treatment Or stirring. Further, purified water can be mixed with a certain amount of these components and stirred or stirred, followed by drying treatment.
According to another embodiment of the present invention, the present invention relates to a composition for a wet tissue comprising the above preservative composition.
The composition for a wet tissue may contain 0.01 wt% to 10 wt%, preferably 0.1 wt% to 1.5 wt% of the preservative composition. When the content of the preservative composition is less than 0.01% by weight, the antibacterial and antifungal effect and persistence are poor. When the content is more than 10% by weight, skin irritation is severe or physical properties are difficult to control.
The composition for a wet tissue may further comprise at least one selected from the group consisting of a pH adjuster, a moisturizer, a perfume, and a combination thereof. Preferably, the composition may contain 0.03 to 0.05% by weight of a pH adjusting agent, 0.2 to 0.7% by weight of a moisturizer, 0.2% by weight.
The humectant may be those conventionally used in the field to which the present invention belongs, and examples thereof include glycerin, butyleneglycol, natural extract, skin components, and the like.
Examples of the pH adjuster include citric acid, sodium citrate, lactic acid, sodium hydroxide, potassium hydroxide, tromethamine, and tie, which are conventionally used in the field to which the present invention belongs.
As the fragrance, those conventionally used in the field to which the present invention belongs can be used, and for example, plant extracts are included.
The composition for a wet tissue may have a pH of 4.5 to 6.0, preferably a pH of 5.0 to 5.8. In the case of the composition for wet tissues containing the preservative composition of the present invention, sulfuric acid is not adsorbed by SDS even in the above-mentioned pH range and the low temperature stability of CPC is excellent. Therefore, the composition can be used stably without changing the physical properties during production of wet tissues, And has an excellent effect.
According to another embodiment of the present invention, the present invention relates to a cosmetic composition comprising said preservative composition.
The cosmetic composition may contain the preservative composition in an amount of 0.1% 10% by weight, and preferably 0.1% by weight to 1.5% by weight. When the content of the preservative composition is less than 0.1% by weight, antibacterial and antifungal effect and persistence are inferior. When the content exceeds 10% by weight, skin irritation is severe or physical properties are difficult to control.
The cosmetic composition may further comprise at least one selected from the group consisting of a pH adjuster, a moisturizer, a perfume, a surfactant, other additives, purified water, and combinations thereof depending on the formulation or physical properties of the cosmetic composition to be produced, 0.01 to 30% by weight of a pH adjusting agent, 0.01 to 30% by weight of a humectant, 0.01 to 3.0% by weight of a perfume, 0.01 to 30% by weight of a surfactant, and other additives and purified water. In the case of a cosmetic composition which satisfies the above-mentioned range, the antibacterial and antifungal effects of the present invention are excellent, and the stability of physical properties is excellent.
According to another embodiment of the present invention, the present invention relates to a shampoo composition comprising said preservative composition.
Wherein the shampoo composition comprises the preservative composition in an amount of 0.1% 10 By weight, and preferably 0.1% by weight to 1.5% by weight. When the content of the preservative composition is less than 0.1% by weight, the antibacterial and antifungal effect and persistence are inferior. When the content exceeds 10% by weight, skin irritation is severe or shampoo properties are difficult to control.
The shampoo composition may further comprise at least one selected from the group consisting of a pH adjuster, a moisturizer, a perfume, a surfactant, other additives, purified water, and combinations thereof. Preferably 0.01 to 10.0% by weight of a pH adjusting agent, 0.01 to 30% by weight of a moisturizer, 0.01 to 3.0% by weight of a perfume, 0.01 to 30% by weight of a surfactant, and other additives and purified water . In the case of a cosmetic composition which satisfies the above-mentioned range, the antibacterial and antifungal effects of the present invention are excellent, and the stability of physical properties is excellent.
According to another embodiment of the present invention, the present invention relates to a detergent composition comprising the preservative composition.
The detergent composition may contain the preservative composition in an amount of 0.1 wt% to 10 wt%, preferably 0.1 wt% to 1.5 wt%. When the content of the preservative composition is less than 0.1% by weight, the antibacterial and antifungal effect and persistence are poor. When the content is more than 10% by weight, skin irritation is severe or physical properties are difficult to control.
The detergent composition may further comprise at least one selected from the group consisting of a pH adjuster, a moisturizer, a perfume, a surfactant, a defoamer, other additives, purified water, and combinations thereof. Preferably 0.01 to 10.0% by weight of a pH adjusting agent, 0.01 to 30% by weight of a moisturizer, 0.01 to 3.0% by weight of a perfume, 0.01 to 30% by weight of a surfactant, 0.01 to 1% Other additives and purified water. In the case of a composition for a detergent satisfying the above range, the antibacterial and antifungal effects of the present invention are excellent, and the stability of physical properties is excellent.
According to another embodiment of the present invention, the present invention relates to a composition for a fabric softener comprising the preservative composition.
The composition for fabric softeners may contain the preservative composition in an amount of 0.1% 10% by weight. When the content of the preservative composition is less than 0.1% by weight, antibacterial and antifungal effects and persistence are inferior. When the content is more than 10% by weight, skin irritation is severe or physical properties of the fabric softener are difficult to control.
The composition for the fabric softener may further include any one selected from the group consisting of a pH adjuster, a moisturizer, a perfume, a surfactant, a defoamer, other additives, purified water, and combinations thereof. Preferably 0.01 to 10.0% by weight of a pH adjusting agent, 0.01 to 30% by weight of a moisturizer, 0.01 to 3.0% by weight of a perfume, 0.01 to 30% by weight of a surfactant, 0.01 to 1% Other additives and purified water. In the case of the fabric softener composition satisfying the above range, the antibacterial and antifungal effects of the present invention are excellent, and the stability of physical properties is excellent.
According to another embodiment of the present invention, the present invention relates to an industrial detergent composition comprising said preservative composition.
The industrial detergent composition comprises the preservative composition in an amount of from 0.1% 10% by weight, and preferably 0.1% by weight to 5% by weight. When the content of the preservative composition is less than 0.1% by weight, antibacterial and antifungal effects and persistence are inferior. When the content exceeds 10% by weight, it is difficult to control the properties of the industrial detergent composition.
The industrial detergent composition may further include any one selected from the group consisting of the pH adjuster, humectant, fragrance, surfactant, defoamer, other additives, purified water, and combinations thereof. Preferably 0.01 to 10.0% by weight of a pH adjusting agent, 0.01 to 30% by weight of a moisturizer, 0.01 to 3.0% by weight of a perfume, 0.01 to 30% by weight of a surfactant, 0.01 to 1% Other additives and purified water. In the case of the composition for industrial cleaning satisfying the above range, the antibacterial and antifungal effects of the present invention are excellent, and the stability of the physical properties is excellent.
According to another embodiment of the present invention, the present invention provides an article comprising the preservative composition, wherein the article includes diapers, sanitary articles, cosmetics, detergents, industrial detergents, disinfectants and sanitary articles. The sanitary article may be, for example, an oral tissue, a mouthwash, a shampoo, a soap, a shower gel, or the like.
The above-mentioned article preferably contains the preservative composition of the present invention in an amount of 0.1% by weight to 10% by weight based on the total weight of the article. When the preservative composition is less than 0.1% by weight based on the total weight of the article, the antibacterial and antifungal effect is insufficient. When the preservative composition is more than 10% by weight, physical properties of the product and skin irritation may be caused.
The article comprising the preservative composition of the present invention can be prepared according to the method of manufacturing an article in the field of the present invention, for example, by mixing the preservative composition of the present invention with an additional ingredient, And then treating the preservative composition of the present invention with the article of manufacture in the final step.
As the method for treating the preservative composition, any of conventionally used methods may be used. For example, the preservative composition may be sprayed on the article, the impregnated article may be impregnated into the preservative composition, And then the preservative composition is added and mixed.
When the article is cosmetics, the preservative composition of the present invention may be added to a certain stage of the manufactured article manufacturing process, and the mixture may be mixed by a method such as an agitator.
When the article is a detergent, in the case of producing a detergent in a liquid state, the preservative composition of the present invention may be added to a certain stage of the manufacturing process of the manufactured article and mixed by a method such as an agitator. , The preservative composition of the present invention may be added to a certain stage of the manufacturing process of the manufactured article, followed by mixing and drying using a stirrer or the like, followed by drying and disintegration.
In the case where the article is a diaper or a feminine hygiene article, it is mostly fabricated in a fiber form, and the preservative composition of the present invention may be added to a certain stage of the article manufacturing process, followed by impregnation, bonding and drying.
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
EXAMPLES Preparation of preservative compositions
Sodium Metabisulfite (SDS) and Cetylpyridinium chloride (CPC) were each dissolved in an appropriate amount of purified water as shown in Table 1 below and mixed at room temperature to prepare 1 kg of the preservative composition of the present invention . In this experiment, an ion exchange resin was used and purified water with a calcium weight of 3 ppm or less was used.
Sodium Metabisulfite (ppm)
Cetylpyridinium chloride (ppm)
Comparative Example 1
1000
0
Example 1
1000
200
Example 2
1000
250
Example 3
1000
500
Example 4
1000
2500
Example 5
1000
4000
Example 6
1000
5000
Comparative Example 2
0
1000
In addition, 1 kg of the preservative composition of the present invention was prepared by dissolving SDS, CPC, sodium sulfite and hexylene glycol in an appropriate amount of purified water in the composition shown in Table 2 below and mixing at room temperature . In this experiment, an ion exchange resin was used and purified water with a calcium weight of 3 ppm or less was used.
[Weight ratio]
Example 7
Example 8
Example 9
Example 10
Sodium Metabisulfite
10
10
10
10
Cetylpyridinium chloride
40
40
40
40
Sodium sulfite
0
10
0
5
Hexylene Glycol
0
0
10
5
Purified water
80
80
80
80
Further, SDS, CPC, sodium sulfite, SS, Hexylene Glycol, HG and various surfactants were dissolved in an appropriate amount of purified water in the compositions shown in Table 3 below, mixed at room temperature, 1 kg of the inventive preservative composition was prepared. In this experiment, an ion exchange resin was used and purified water with a calcium weight of 3 ppm or less was used.
[Weight ratio]
SDS
CPC
SS
HG
Surfactant, weight ratio
Purified water
Example 11
2
8
5
5
Sodium lauryl sulfate, 5
75
Example 12
2
8
5
5
Sodium xylene sulfonate, 5
75
Example 13
2
8
5
5
Ammonium laureth sulfate, 5
75
Example 14
2
8
5
5
Disodium laureth sulfosuccinate, 5
75
Example 15
2
8
5
5
Sodium lauroisocyanate, 5
75
Example 16
2
8
5
5
Laureus-7, 5
75
Example 17
2
8
5
5
Laureates-9, 5
75
Example 18
2
8
5
5
Coco-glucoside, 5
75
Example 19
2
8
5
5
Polysorbate 20, 5
75
Example 20
2
8
5
5
Polysorbate 60, 5
75
Example 21
2
8
5
5
Disodium cocoamphodiacetate, 5
75
Example 22
2
8
5
5
Cocamidopropyl betaine, 5
75
Preparation Example 1 Preparation of a wet tissue containing the preservative composition of the present invention
The wet tissue compositions were prepared using the preservative compositions prepared as in Examples 1 to 6 and Comparative Examples 1 and 2 in Table 1 above. The preservative compositions shown in Table 1 were added to purified water and stirred, and further, fragrances, extracts, and moisturizing agents were added in any order, and the mixture was stirred at room temperature.
As the fragrance, 0.01 wt% of citric acid, 0.1 wt% of marigold extract and 0.5 wt% of glycerin as a moisturizing agent were added. After adding the fragrance, the extract and the moisturizing agent, 0.05 wt% of citric acid, sodium citrate and other pH adjusting agent was added as a pH adjusting agent, and the pH was adjusted to 4.8 to 5.8 to prepare a final product, a wet tissue composition.
The wet tissue composition thus prepared was sprayed onto a fabric with a spray device when the wet tissue was passed through a converting machine through a wet tissue manufacturing machine, and the applied wet tissue was trimmed to prepare a wet tissue.
PREPARATION EXAMPLE 2 Preparation of Cosmetic Composition Containing the Preservative Composition of the Present Invention
Cosmetic compositions containing the preservative compositions of Examples 1 to 6 and Comparative Examples 1 to 2 were prepared with the composition shown in Table 4 below.
No.
Trade Name
INCI Name
Content (% by weight)
One
DC9040
Cyclopentasiloxane (and) Dimethicone Crosspolymer
One
2
SF0005Z
Cyclopentasiloxane
10
3
Cegesoft SB 45
Shea Butter
1.5
4
Olive Oil
Olea Europaea (Olive) Fruit Oil
0.5
5
Myritol 312
Caprilic / Capric Trigliceride
2
6
Cegesoft C24W
Ethylhexyl Palmitate
3
7
Abil EM 90
Cetyl PEG / PPG-10/1 dimethicone
1.2
8
D.I Water
Water
to 100
9
Niacineamide
Niacineamide
5
10
EDTA2Na
Disodium EDTA
0.02
11
Keltrol-F
Xanthan Gum
0.2
12
Glycerin
Glycerin
5
13
1.3 BG
1.3 BG
5
14
Sodium hyaluronate
Sodium hyaluronate
0.05
15
Preservative composition
Examples 1-6 and Comparative Examples 1-2
1.0
Specifically, of the components listed in Table 4, 9 to 11 were stirred at 2500 rpm Disper at 78 캜 for 10 minutes. 1 to 8 were also mixed at 78 ° C., mixed with each other and stirred at 4000 rpm for 15 minutes at 78 ° C. with a homomixer. Then, the emulsion was prepared by dissolving the fragrance and the preservative composition prepared in the Example uniformly at a temperature of 40 DEG C or lower.
PREPARATION EXAMPLE 3 Preparation of Shampoo Containing the Preservative Composition of the Present Invention
A shampoo containing the preservative compositions of Examples 1 to 6 and Comparative Examples 1 to 2 was prepared with the composition shown in Table 5 below.
No.
Trade Name
INCI Name
Content (% by weight)
One
Water
Water
q.s
2
MICONIUM PQ10 J-3000
Polyquaternium-10
0.25
3
EDTA-2NA
Disodium iodide
0.10
5
Miconol-C2M
Disodium cocoamphodiacetate
15.00
6
Mitain-CA (S)
Cocamidopropyl betaine
7.00
7
Miconate-LES
Disodium laureth sulfosuccinate
7.00
8
Plantacare-2000UP
Decyl glucoside
5.00
9
1,3-Butylene Glycol
Butylene glycol
2.00
10
DOE-120
≪ RTI ID = 0.0 > phage-120 <
0.50
11
CROTHIX-PA- (MH)
PGE-150 Pentaerythritol tetrastearate
0.50
12
Phospholipid-EFA
Linoleamidopropylphi-dimonium chloride phosphate
1.00
13
Gluadin-WLM BENZ
Hydrolized milk protein
0.30
14
D-Panthenol
Panthenol
0.20
15
XIAMETER® MEM-1870 EMULSION
Dimethicone
1.00
16
Sodium Xylenesulfonate
Sodium xylene sulfonate
0.50
17
Fragrance
Spices
1.20
18
Jojoba-Oil
Jojoba seed oil
0.50
19
Purac HS-88
Lactic Acid
0.30
20
Preservative composition
Examples 1-6 and Comparative Examples 1-2
1.0
Specifically, 1 to 3 times of stirring were carried out in this order, stirring was continued at 40 ° C until transparency, and stirring was continued until 5 to 8 times. Then, 9 to 11 were preliminarily mixed at 50 ° C, put into the main mix, and gradually added while being cooled gradually from 12th. The shampoo was prepared by buffering at a pH of 5 to 6.
Preparation Example 4 Preparation of a laundry detergent containing the preservative composition of the present invention
A laundry detergent containing the preservative compositions of Examples 1 to 6 and Comparative Examples 1 to 2 was prepared with the composition shown in Table 6 below.
No.
Trade Name
INCI Name
content(%)
One
Purified water
WATER
to 100
2
LAS
linear alkyl benzene sulfonate
12,000
3
DEA
Cocamide DEA
4.500
4
SLES
Sodium Laureth Sulfate
1,000
5
Glucopon-650 EC
Akylpolyglucocide
0.500
6
EDTA-4NA
Tetrasodium EDTA
0.100
7
Red No. 40
BLUE 1
0.000
8
Citric acid
Citric acid
0.010
9
Grapefruit extract
Citrus Paradisi (Grapefruit) Fruit Extract
0.010
10
Green tea extract
Camellia Sinensis Leaf Extrac
0.010
11
incense
Fragrance
0.100
12
DB-110A
Dimethicone
0.100
13
Preservative composition
Examples 1-6 and Comparative Examples 1-2
1.0
Preparation Example 5 Preparation of a fabric softener containing the preservative composition of the present invention
A fabric softening agent containing the preservative compositions of Examples 1 to 6 and Comparative Examples 1 and 2 was prepared with the composition shown in Table 7 below.
No.
Trade Name
INCI Name
content(%)
One
Purified water
WATER
to 100
2
EDTA-4NA
Tetrasodium EDTA
0.100
3
Hisofter MEQ-80
Dihydrogenated Palmoyl Hydroxyethylmonium Methosulfate
3.0000
4
HONEOL LAE-15
Laureth-15
0.7000
5
MEG
Ethylene Glycol
3.0000
6
Citric acid
Citric acid
0.020
7
NaCl
NaCl
0.1000
8
incense
Fragrance
0.5000
9
DB-110A
Dimethicone
0.1000
10
Preservative composition
Examples 1-6 and Comparative Examples 1-2
1.0
≪ Preparation Example 6 > Preparation of an industrial degreaser containing the preservative composition of the present invention
Industrial grease removing agents containing the preservative compositions of Examples 1 to 6 and Comparative Examples 1 to 2 were prepared with the composition shown in Table 8 below.
No.
Trade Name
content(%)
One
Water
To 100
2
Tetrasodium EDTA, 38%
10
3
Sodium metasilicate anhydrous
5
4
PEG-16 tallow ethyl ammonium ethosulfate
3
5
Alcohol Ethoxylate
4
6
Antiseptic composition, Examples 1-6, Comparative Examples 1-2
1.0
<Experimental Example 1> Antibacterial and antifungal effect of the preservative composition of the present invention
The antimicrobial and antifungal test of the antiseptic and antifungal test of the preservative composition of the present invention can be carried out by using Shaking Flask Method in the case of Escherichia coli , Staphylococcus aureus and Pseudomonas aeruginosa , 100 ml of nutrient broth, Fungus Aspergillus niger , In the case of Candida albicans , the strain cultured in 100 mL of Potato dextrose broth was inoculated with platinum. The inoculated medium was incubated at 30 ° C for 24 hours in a shaking incubator. To each aqueous solution of the 0.9% NaCl solution to which no preservative had been added, the preservative compositions of Examples 1 to 6 and Comparative Examples 1 and 2 were added in an amount of 1.00% by weight and the same amount of purified water was added to the control (Control) Tube was treated with 10 6 CFU / ml of Escherichia coli , 10 6 CFU / ml Staphylococcus aureus , 10 6 CFU / ml of Pseudomonas aeruginosa, 10 5 CFU / ml of fungus the Aspergillus niger) and Candida albicans (candida albicans) were each vaccinated. Then, 1 mL of each solution was mixed with 0.9 mL of 0.9% NaCl solution, and 1 mL of the solution was added dropwise to the petri-dish in triplicate by a spreading coating method. Escherichia coli and Staphylococcus aureus and Pseudomonas aeruginosa were incubated in a constant temperature incubator at 35 ° C for 2 days while Aspergillus niger and Candida albicans were incubated in a constant temperature incubator at 25 ° C for 3 days. After incubation for about one day, the number of colonies was counted and multiplied by a dilution factor to make a number of bacteria per 1 mL. The results are shown in Table 9 below.
Elapsed Days
Test strain
Inoculation number
Control group
Comparative Example 1
Example 1
Example 2
Example 3
Example 4
Example 5
Example 6
Comparative Example 2
The strain (CFU / ml)
Day 3
Escherichia coli
1.3 x 10 6
4
One
0
0
0
0
0
0
One
Staphylococcus aureus
1.04 x 10 6
4
One
0
0
0
0
0
0
One
P. aeruginosa
3.4 x 10 6
4
One
0
0
0
0
0
0
One
Candida albicans
1.48 x 10 5
4
2
0
0
0
0
0
0
2
Black fungus
1.9 x 10 5
4
2
0
0
0
0
0
0
One
Day 7
Escherichia coli
1.3 x 10 6
4
0
0
0
0
0
0
0
One
Staphylococcus aureus
1.04 x 10 6
4
0
0
0
0
0
0
0
One
P. aeruginosa
3.4 x 10 6
4
0
0
0
0
0
0
0
One
Candida albicans
1.48 x 10 5
4
2
0
0
0
0
0
0
3
Black fungus
1.9 x 10 5
4
2
0
0
0
0
0
0
One
0 = No Contamination (Sterile)
1 = Trace of contamination (1 ~ 9 Colonies per streak)
2 = Light contamination (10 ~ 99 Colonies per streak)
3 = Moderate contamination (Greater than 100 colonies but still distinguishable)
4 = Heavy contamination
As shown in the above Table 8, the preservative compositions of the present invention (Examples 1 to 6) in which sodium metabisulfite and cetylpyridinium chloride were mixed are superior to the preservative composition using SDS or CPC alone The antimicrobial and antifungal activity effects were observed. In particular, the preservative compositions of Examples 1 to 6 were found to have an excellent antibacterial and antifungal effect of 99.9% or more in all the various strains.
<Experimental Example 2> Confirmation of effectiveness of the wet tissue including the preservative composition of the present invention
The antibacterial effect of the wet tissue prepared in Preparation Example 1 was tested, and the results are shown in Table 10. As a control (control), a wet tissue was prepared using the same wet tissue composition prepared in Preparation Example 1 by adding the same amount of purified water to the preservative composition of the present invention.
Elapsed Days
Test strain
Inoculation number
Control group
Comparative Example 1
Example 1
Example 2
Example 3
Example 4
Example 5
Example 6
Comparative Example 2
The strain (CFU / ml)
Day 2
Escherichia coli
2.5 x 10 6
3
2
2
2
2
One
0
0
2
Staphylococcus aureus
1.5 x 10 6
4
2
2
2
2
One
0
0
2
P. aeruginosa
3.1 x 10 6
3
2
2
2
2
One
0
0
3
Candida albicans
1.4 x 10 5
4
One
One
One
One
One
0
0
2
Black fungus
1.9 x 10 5
4
2
2
One
One
One
One
0
3
Day 3
Escherichia coli
2.5 x 10 6
4
2
0
0
0
0
0
0
2
Staphylococcus aureus
1.5 x 10 6
3
One
0
0
0
0
0
0
One
P. aeruginosa
3.1 x 10 6
3
One
0
0
0
0
0
0
One
Candida albicans
1.4 x 10 5
4
One
0
0
0
0
0
0
2
Black fungus
1.9 x 10 5
4
2
0
0
0
0
0
0
One
Day 7
Escherichia coli
2.5 x 10 6
4
One
0
0
0
0
0
0
One
Staphylococcus aureus
1.5 x 10 6
4
0
0
0
0
0
0
0
0
P. aeruginosa
3.1 x 10 6
4
0
0
0
0
0
0
0
0
Candida albicans
1.4 x 10 5
4
0
0
0
0
0
0
0
3
Black fungus
1.9 x 10 5
4
3
0
0
0
0
0
0
One
0 = No Contamination (Sterile)
1 = Trace of contamination (1 ~ 9 Colonies per streak)
2 = Light contamination (10 ~ 99 Colonies per streak)
3 = Moderate contamination (Greater than 100 colonies but still distinguishable)
4 = Heavy contamination
As shown in the above Table 10, the preservative compositions of the present invention (Examples 1 to 6) in which sodium metabisulfite and cetylpyridinium chloride were mixed are superior in antibacterial and antifungal properties to preservative compositions using SDS or CPC alone In particular, it was confirmed that the preservative compositions of Examples 1 to 6 had an excellent antibacterial and antifungal effect of 99.9% or more in all the various strains within 3 days. In particular, in Examples 5 to 6, it was confirmed that the antibacterial and antifungal effects were excellent in 99.9% or more of all the various strains on day 2.
<Experimental Example 3> Confirmation of effect of the cosmetic composition containing the preservative composition of the present invention
The antibacterial and antifungal effect of the cosmetic composition (emulsion) prepared in Preparation Example 2 was confirmed by the following method. Antimicrobial and antifungal test of the antiseptic and antifungal test of the preservative composition of the present invention was carried out according to ASTM D2574 by adding 10 4 CFU / ml Escherichia coli, 10 4 CFU / ml Staphylococcus aureus), 10 4 Pseudomonas aeruginosa (Pseudomonas aeroginosa) a was mixed and streaking, and observe the results after incubation in the initial, 3 days, 7 days at room temperature conditions, 10 Candida albicans (candida albicans of 5 CFU / ml) of CFU / ml And Aspergillus niger were also streaked, and the results were observed after incubation at room temperature for 3 days, 7 days. The control was prepared according to Preparation Example 2 by adding the same amount of purified water to the preservative composition of the present invention. The results of the antibacterial and antifungal effects are shown in Table 11.
Elapsed Days
Test strain
Inoculation number
Control group
Comparative Example 1
Example 1
Example 2
Example 3
Example 4
Example 5
Example 6
Comparative Example 2
The strain (CFU / ml)
Day 2
Escherichia coli
2.8 x 10 6
3
One
2
One
One
One
0
0
2
Staphylococcus aureus
1.6 x 10 6
4
One
One
One
One
One
0
0
2
P. aeruginosa
2.1 x 10 6
3
One
One
One
One
One
0
0
3
Candida albicans
1.6 x 10 5
4
One
One
One
One
One
0
0
2
Black fungus
1.4 x 10 5
4
2
One
One
One
One
0
0
4
Day 3
Escherichia coli
2.8 x 10 6
4
3
0
0
0
0
0
0
2
Staphylococcus aureus
1.6 x 10 6
4
2
0
0
0
0
0
0
2
P. aeruginosa
2.1 x 10 6
4
2
0
0
0
0
0
0
2
Candida albicans
1.6 x 10 5
4
2
0
0
0
0
0
0
3
Black fungus
1.4 x 10 5
4
3
0
0
0
0
0
0
2
Day 7
Escherichia coli
2.8 x 10 6
4
3
0
0
0
0
0
0
One
Staphylococcus aureus
1.6 x 10 6
4
2
0
0
0
0
0
0
2
P. aeruginosa
2.1 x 10 6
4
3
0
0
0
0
0
0
One
Candida albicans
1.6 x 10 5
4
3
0
0
0
0
0
0
3
Black fungus
1.4 x 10 5
4
2
0
0
0
0
0
0
2
0 = No Contamination (Sterile)
1 = Trace of contamination (1 ~ 9 Colonies per streak)
2 = Light contamination (10 ~ 99 Colonies per streak)
3 = Moderate contamination (Greater than 100 colonies but still distinguishable)
4 = Heavy contamination
As shown in the above Table 11, the preservative compositions Examples 1 to 6 of the present invention exhibited an excellent killing rate of 99.9% or more in all the bacteria and fungi on the third day, but the cosmetic composition containing the preservative compositions of Comparative Examples 1 and 2 It was confirmed that the bacteria did not die completely even on day 7 due to insufficient antibacterial activity against bacteria and fungi. Also, it was confirmed that antibacterial and antifungal activity was relatively weak in Comparative Example 1 in which the content of cetylpyridinium chloride was relatively large. Therefore, it was confirmed that the preservative composition of the present invention not only uses the SDS and CPC in a simple mixture, but also has an antibacterial and antifungal effect on the weight ratio. In particular, in Examples 5 to 6, the antifungal agent was killed on day 2.
<Experimental Example 4> Confirmation of effect of the shampoo containing the preservative composition of the present invention
The antibacterial and antifungal effects of the shampoo prepared in Preparation Example 3 were tested in the following manner. As antimicrobial and antifungal test evaluation methods of the preservative composition of the present invention, 10 4 CFU / ml of Escherichia coli , 10 4 CFU / ml Staphylococcus aureus , 10 10 CFU / ml were added to the target shampoo through ASTM D2574 4 CFU / a Pseudomonas aeruginosa (Pseudomonas aeroginosa) of ml and mixed to streaking, initially, was observed with the results 1 day, 3 days, and then cultured in seven days at room temperature conditions, the fungal 10 Candida albicans of 5 CFU / ml (candida albicans) And Aspergillus niger were also streaked, and the results were observed after incubation at room temperature for 3 days, 7 days. The control was prepared according to Preparation Example 3 by adding the same amount of purified water to the preservative composition of the present invention. The test results are shown in Table 12 below.
Elapsed Days
Test strain
Inoculation number
Control group
Comparative Example 1
Example 1
Example 2
Example 3
Example 4
Example 5
Example 6
Comparative Example 2
The strain (CFU / ml)
Day 2
Escherichia coli
2.8 x 10 6
3
One
0
0
0
One
One
One
2
Staphylococcus aureus
1.8 x 10 6
4
One
0
0
0
One
One
One
One
P. aeruginosa
2.9 x 10 6
4
2
0
0
0
One
One
One
One
Candida albicans
1.4 x 10 5
4
3
0
0
One
One
One
One
3
Black fungus
1.9 x 10 5
4
2
0
0
One
One
One
One
One
Day 3
Escherichia coli
2.8 x 10 6
4
3
0
0
0
0
0
0
One
Staphylococcus aureus
1.8 x 10 6
4
2
0
0
0
0
0
0
One
P. aeruginosa
2.9 x 10 6
4
2
0
0
0
0
0
0
0
Candida albicans
1.4 x 10 5
4
2
0
0
0
0
0
0
3
Black fungus
1.9 x 10 5
4
3
0
0
0
0
0
0
One
Day 7
Escherichia coli
2.8 x 10 6
3
3
0
0
0
0
0
0
2
Staphylococcus aureus
1.8 x 10 6
4
2
0
0
0
0
0
0
One
P. aeruginosa
2.9 x 10 6
4
3
0
0
0
0
0
0
One
Candida albicans
1.4 x 10 5
4
3
0
0
0
0
0
0
3
Black fungus
1.9 x 10 5
4
2
0
0
0
0
0
0
One
0 = No Contamination (Sterile)
1 = Trace of contamination (1 ~ 9 Colonies per streak)
2 = Light contamination (10 ~ 99 Colonies per streak)
3 = Moderate contamination (Greater than 100 colonies but still distinguishable)
4 = Heavy contamination
As shown in Table 12, in the case of the shampoo containing the preservative compositions of Examples 1 to 6, all bacteria and fungi were excellent in the mortality rate of 99.9% or more on the third day, In the shampoo composition containing the preservative composition, the antibacterial ability against bacteria and fungi was insufficient, so that bacteria and fungi could not be completely killed even on day 7. Therefore, it was confirmed that, when shampoos were mixed with SDS and CPC of the present invention at a specific ratio, a preservative composition having better antibacterial and antifungal effects than those using only one component could be produced. In particular, in Examples 1 and 2, it was confirmed that the antifungal agent was killed on day 2.
<Experimental Example 5> Confirmation of the effect of the laundry detergent containing the preservative composition of the present invention
The antibacterial and antifungal effect of the laundry detergent prepared in Preparation Example 4 was confirmed by the following method. E. coli (Escherichia coli), 10 6 ( Staphylococcus aureus) Staphylococcus aureus CFU / ml of the present invention, antiseptic antibacterial and antifungal capacity test and evaluation methods as is 10 6 CFU / ml to the target substance through the ASTM D2574 method detergent of the composition, 10 6 CFU / mixed by streaking a Pseudomonas aeruginosa (Pseudomonas aeroginosa) in ml, and the initial, 3 days, 7 days was observed after incubation results from room temperature conditions, 10 5 CFU / ml Candida albicans (candida albicans) and black mold spores of ( Aspergillus niger ) were streaked, and the results were observed after incubation at room temperature for 3 days, 7 days. The control (control) was prepared according to Preparation Example 4 by adding the same amount of purified water to the preservative composition of the present invention, and the test results are shown in Table 13.
Elapsed Days
Test strain
Inoculation number
Control group
Comparative Example 1
Example 1
Example 2
Example 3
Example 4
Example 5
Example 6
Comparative Example 2
The strain (CFU / ml)
Day 2
Escherichia coli
3.3 x 10 6
2
One
0
0
0
0
0
One
2
Staphylococcus aureus
2.8 x 10 6
3
One
0
0
0
0
0
0
One
P. aeruginosa
2.1 x 10 6
3
2
0
0
0
0
0
0
One
Candida albicans
2.4 x 10 5
3
3
0
0
One
0
One
One
3
Black fungus
3.2 x 10 5
4
2
0
0
One
One
One
One
One
Day 3
Escherichia coli
3.3 x 10 6
4
One
0
0
0
0
0
0
One
Staphylococcus aureus
2.8 x 10 6
4
0
0
0
0
0
0
0
One
P. aeruginosa
2.1 x 10 6
4
0
0
0
0
0
0
0
0
Candida albicans
2.4 x 10 5
4
0
0
0
0
0
0
0
3
Black fungus
3.2 x 10 5
4
3
0
0
0
0
0
0
One
Day 7
Escherichia coli
3.3 x 10 6
2
3
0
0
0
0
0
0
2
Staphylococcus aureus
2.8 x 10 6
3
0
0
0
0
0
0
0
One
P. aeruginosa
2.1 x 10 6
3
0
0
0
0
0
0
0
One
Candida albicans
2.4 x 10 5
3
One
0
0
0
0
0
0
3
Black fungus
3.2 x 10 5
2
3
0
0
0
0
0
0
One
0 = No Contamination (Sterile)
1 = Trace of contamination (1 ~ 9 Colonies per streak)
2 = Light contamination (10 ~ 99 Colonies per streak)
3 = Moderate contamination (Greater than 100 colonies but still distinguishable)
4 = Heavy contamination
As shown in Table 13, in the case of the laundry detergent containing the preservative compositions of Examples 1 to 6 of the present invention, all bacteria and fungi were excellent in the killing rate of 99.9% or more on the third day, , The antibacterial activity against bacteria and fungi was insufficient, so that it was not completely killed even on day 7. Thus, it was confirmed that when the SDS and CPC of the present invention were mixed in a laundry detergent at a specific ratio, a preservative composition having more excellent antibacterial and antifungal effect than those using only one component could be produced. In particular, in Examples 1 and 2, it was confirmed that all the fungi died even on the second day.
<Experimental Example 6> Confirmation of the effect of the fabric softener containing the preservative composition of the present invention
The antibacterial and antifungal effects of the fabric softener prepared in Preparation Example 5 were tested in the following manner. E. coli (Escherichia coli), 10, 6 (Staphylococcus aureus) Staphylococcus aureus CFU / ml of the present invention, antiseptic antibacterial and antifungal capacity test and evaluation methods as is 10 6 CFU / ml to the target substance through the ASTM D2574 method detergent of the composition, 10 6 CFU / mixed by streaking a Pseudomonas aeruginosa (Pseudomonas aeroginosa) in ml, and the initial, 3 days, 7 days was observed after incubation results from room temperature conditions, 10 5 CFU / ml Candida albicans (candida albicans) and black mold spores of ( Aspergillus niger ) were streaked, and the results were observed after incubation at room temperature for 3 days, 7 days. The control (control) was prepared according to Preparation Example 5 by adding the same amount of purified water to the preservative composition of the present invention, and the test results are shown in Table 14.
Elapsed Days
Test strain
Inoculation number
Control group
Comparative Example 1
Example 1
Example 2
Example 3
Example 4
Example 5
Example 6
Comparative Example 2
The strain (CFU / ml)
Day 2
Escherichia coli
2.5 x 10 6
2
2
0
0
0
0
0
One
2
Staphylococcus aureus
2.1x 10 6
2
2
0
0
0
0
0
0
2
P. aeruginosa
4.2 x 10 6
2
2
0
0
0
0
0
0
2
Candida albicans
1.9 x 10 5
2
One
0
0
One
One
One
One
3
Black fungus
3.5 x 10 5
4
2
0
0
One
One
One
One
One
Day 3
Escherichia coli
2.5 x 10 6
2
2
0
0
0
0
0
0
2
Staphylococcus aureus
2.1x 10 6
One
One
0
0
0
0
0
0
One
P. aeruginosa
4.2 x 10 6
One
One
0
0
0
0
0
0
One
Candida albicans
1.9 x 10 5
2
2
0
0
0
0
0
0
2
Black fungus
3.5 x 10 5
One
One
0
0
0
0
0
0
One
Day 7
Escherichia coli
2.5 x 10 6
One
One
0
0
0
0
0
0
One
Staphylococcus aureus
2.1x 10 6
One
One
0
0
0
0
0
0
One
P. aeruginosa
4.2 x 10 6
2
2
0
0
0
0
0
0
2
Candida albicans
1.9 x 10 5
One
One
0
0
0
0
0
0
One
Black fungus
3.5 x 10 5
One
One
0
0
0
0
0
0
One
0 = No Contamination (Sterile)
1 = Trace of contamination (1 ~ 9 Colonies per streak)
2 = Light contamination (10 ~ 99 Colonies per streak)
3 = Moderate contamination (Greater than 100 colonies but still distinguishable)
4 = Heavy contamination
As shown in Table 14, in the case of the fabric softener composition comprising the preservative compositions of Examples 1 to 6 of the present invention, all bacteria and fungi were excellent in the killing rate of 99.9% 2 fabric softener composition was insufficient in antibacterial activity against bacteria and fungi, and was not completely killed on day 7. Therefore, it was confirmed that when the SDS and CPC of the present invention are mixed in a specific ratio, a preservative composition having antibacterial and antifungal effects can be produced even more than when only one component is used. In particular, it was confirmed that all of the fungi were killed on the 2nd day in Examples 1 and 2.
≪ Experimental Example 7 > Preparation of an industrial grease removing agent containing the preservative composition of the present invention.
The antibacterial and antifungal effects of the industrial degreasing agent prepared in Preparation Example 6 were tested in the following manner. E. coli (Escherichia coli), 10 6 ( Staphylococcus aureus) Staphylococcus aureus CFU / ml of the present invention, antiseptic antibacterial and antifungal capacity test and evaluation methods as is 10 6 CFU / ml to the target substance through the ASTM D2574 method detergent of the composition, 10 6 CFU / aeruginosa in ml and mixed with streaking (Pseudomonas aeroginosa), respectively, and initially, 3 days, 7 days was observed after incubation results from room temperature conditions, 10 5 Candida albicans (candida albicans) and black mold of CFU / ml ( Aspergillus niger ) were also streaked, and the results were observed after incubation at room temperature for 1 day, 3 days, and 7 days. As a control, an industrial degreaser prepared according to Preparation Example 6 was prepared by adding the same amount of purified water to the preservative composition of the present invention, and the test results are shown in Table 15.
Elapsed Days
Test strain
Inoculation number
Control group
Comparative Example 1
Example 1
Example 2
Example 3
Example 4
Example 5
Example 6
Comparative Example 2
The strain (CFU / ml)
Day 2
Escherichia coli
3.5 x 10 6
3
3
0
One
0
0
0
0
2
Staphylococcus aureus
2.3x 10 6
3
3
One
One
One
One
0
0
2
P. aeruginosa
4.1 x 10 6
3
2
0
One
0
0
0
0
2
Candida albicans
1.9 x 10 5
3
3
One
One
One
One
0
0
3
Black fungus
3.8 x 10 5
4
3
One
One
One
One
0
0
One
Day 3
Escherichia coli
3.5 x 10 6
3
2
0
0
0
0
0
0
2
Staphylococcus aureus
2.3x 10 6
2
One
0
0
0
0
0
0
One
P. aeruginosa
4.1 x 10 6
3
One
0
0
0
0
0
0
One
Candida albicans
1.9 x 10 5
2
2
0
0
0
0
0
0
2
Black fungus
3.8 x 10 5
2
One
0
0
0
0
0
0
One
Day 7
Escherichia coli
3.5 x 10 6
3
One
0
0
0
0
0
0
One
Staphylococcus aureus
2.3x 10 6
2
One
0
0
0
0
0
0
One
P. aeruginosa
4.1 x 10 6
3
One
0
0
0
0
0
0
2
Candida albicans
1.9 x 10 5
2
One
0
0
0
0
0
0
One
Black fungus
3.8 x 10 5
2
2
0
0
0
0
0
0
One
0 = No Contamination (Sterile)
1 = Trace of contamination (1 ~ 9 Colonies per streak)
2 = Light contamination (10 ~ 99 Colonies per streak)
3 = Moderate contamination (Greater than 100 colonies but still distinguishable)
4 = Heavy contamination
As shown in Table 15, in the case of the industrial degreaser composition containing the preservative compositions of Examples 1 to 6 of the present invention, all bacteria and fungi were excellent in the killing rate of 99.9% or more at the third day, In the fabric softener compositions of Examples 1 to 2, the antimicrobial activity against bacteria and fungi was insufficient, and it was not completely killed even on day 7. Therefore, it was confirmed that, when the SDS and CPC of the present invention are mixed in a specific ratio in an industrial degreaser, it is possible to produce a preservative composition having better antibacterial and antifungal effects than those using only one component.
In particular, in Examples 5 to 6, both bacteria and fungi were killed on day 2.
<Experimental Example 8> Confirmation of stability of the preservative composition of the present invention
As in Examples 7 to 10 of Table 2 and Examples 11 to 22 of Table 3, each preservative containing SDS, CPC, sodium sulfite, hexylene glycols and various surfactants The effect of the composition on the freezing point and the recrystallization temperature drop was confirmed.
The temperature (recrystallization temperature) at which the fine crystal lattice of the pear-shaped cloud phenomenon occurs is measured by dropping the temperature of the 100 ml solution with the cooling water. These results are shown in Table 16 below.
Freezing Point O C
Recrystallization temperature O C
Freezing Point O C
Recrystallization temperature O C
Example 7
2.0
10.0
Example 15
-22.0
0.1
Example 8
-3.0
7.0
Example 16
-21.0
0.3
Example 9
-11.0
6.0
Example 17
-22.0
0.3
Example 10
-14.0
4.0
Example 18
-23.0
0.1
Example 11
-15.0
2.0
Example 19
-22.0
0.3
Example 12
-16.0
1.5
Example 20
-22.0
0.2
Example 13
-16.5
1.0
Example 21
-21.0
0.5
Example 14
-20.0
0.5
Example 22
-21.0
0.5
As shown in Table 16 above, the preservative composition may be prepared by mixing any one selected from the group consisting of sodium sulfite, hexylene glycol, surfactant, and combinations thereof by mixing only SDS and CPC to prepare a preservative composition , The recrystallization temperature and freezing point were lowered, and it was confirmed that a preservative composition having excellent stability could be produced.
EXPERIMENTAL EXAMPLE 9 Confirmation of skin hypoactivity effect of the preservative composition of the present invention
In order to investigate the irritation to the skin of the preservative composition of the present invention, the following experiment was conducted. In Examples 1 to 22, a solution containing purified water was prepared, and a Finn chamber; 10 μl of each sample was absorbed into a paper disc of NORGESPLASTER A / S and NORWAY, and the sample was adhered to the lower part of the arm (20 persons) for 48 hours. After finishing the Finn chamber and rinsing with flowing water, skin fogging was examined 2 hours later. The results are shown in Table 17.
division
Total number of subjects / 20
++
+
±
-
Example 1
-
-
-
20
Example 2
-
-
-
20
Example 3
-
-
-
20
Example 4
-
-
-
20
Example 5
-
-
-
20
Example 6
-
-
-
20
Example 7
-
-
-
20
Example 8
-
-
-
20
Example 9
-
-
-
20
Example 10
-
-
-
20
Example 11
-
-
-
20
Example 12
-
-
-
20
Example 13
-
-
-
20
Example 14
-
-
-
20
Example 15
-
-
-
20
Example 16
-
-
-
20
Example 17
-
-
-
20
Example 18
-
-
-
20
Example 19
-
-
-
20
Example 20
-
-
-
20
Example 21
-
-
-
20
Example 22
-
-
-
20
As shown in Table 17, it was confirmed that the preservative compositions of Examples 1 to 22 were all free of skin irritation.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit of the invention as set forth in the appended claims. The present invention can be variously modified and changed by those skilled in the art, and it is also within the scope of the present invention.