CN109498535B

CN109498535B - Bacteriostatic composition, bacteriostatic no-wash gel, and preparation method and application thereof

Info

Publication number: CN109498535B
Application number: CN201811519234.1A
Authority: CN
Inventors: 郑钦方; 汪冶
Original assignee: Huaihua Wulingsan Dong Pharmaceutical Technology Development Co ltd
Current assignee: Hunan University of Medicine
Priority date: 2018-12-12
Filing date: 2018-12-12
Publication date: 2021-10-26
Anticipated expiration: 2038-12-12
Also published as: CN109498535A

Abstract

The invention relates to a bacteriostatic composition, a bacteriostatic no-wash gel and a preparation method thereof, wherein the bacteriostatic composition comprises a first component, the first component comprises essential oil of leaves of cinnamomum japonicum and essential oil of leaves of cinnamomum camphora, and the mass ratio of the essential oil of leaves of cinnamomum japonicum to the essential oil of leaves of cinnamomum camphora is 1 (0.5-2). The antibacterial composition has strong bactericidal and bacteriostatic effects, is mild and non-irritant, and can be widely used for preparing daily necessities.

Description

Bacteriostatic composition, bacteriostatic no-wash gel, and preparation method and application thereof

Technical Field

The invention relates to the technical field of daily necessities, in particular to a bacteriostatic composition, a bacteriostatic no-wash gel and a preparation method and application thereof.

Background

Various microorganisms exist in nature, and part of the microorganisms entering the human body can have great influence on the environment in the human body and even cause various infectious diseases. With the development of society, people pay more and more attention to health, and begin to use various disinfection products to disinfect food, bodies, clothes and the like, but most of the products need to be washed by water, and are inconvenient for regions with poor water resources or people going out, so that a series of wash-free products are gradually created, and the wash-free gel is particularly popular.

The disposable gel is a disposable sanitary product made of gel base material packages, has the basic functions of disinfection and skin cleaning, is convenient to carry and the like, and gradually becomes an essential cleaning product in daily life of people. The safety of the product is very important due to no water flushing, and many products in the current market add some chemical synthetic substances for improving the sterilization effect, the substances generally have strong irritation and are easy to cause allergy, the substances are difficult to metabolize in vivo, and a large amount of residues can generate great hidden troubles, so the application of the products is limited.

Disclosure of Invention

Therefore, the bacteriostatic composition, the bacteriostatic no-wash gel and the preparation method and application thereof are needed to be provided, wherein the bacteriostatic composition is mild and non-irritant and has a good bactericidal effect.

The bacteriostatic composition is provided with a first component, wherein the first component comprises camphor leaf essential oil of Hongya and camphor leaf essential oil of borneol, and the mass ratio of the camphor leaf essential oil of Hongya to the camphor leaf essential oil of borneol is 1 (0.5-2).

The antibacterial composition is prepared by mixing the essential oil of the leaves of the Hongya camphor trees and the essential oil of the leaves of the borneol camphor trees in a specific proportion to form a first component. The borneol camphor essential oil is the crude borneol of the dextro-borneol obtained by distilling the borneol camphor or the crude camphor obtained by centrifuging the crude borneol, has the functions of clearing heat, relieving pain, preventing corrosion and relieving itching when being externally used, can treat diseases such as pyocutaneous disease, swelling and pain, aphtha and the like, has the effects of inducing resuscitation and refreshing when being orally taken, and is suitable for various syndromes of unconsciousness and convulsion.

The cinnamomum japonicum camphora is a tree species of evergreen trees in cinnamomum of Lauraceae, the cinnamomum japonicum camphora leaves are rich in camphora leaf essential oil, and the extracted essential oil is widely applied to industries such as medicine, essence, spice, cosmetics and the like and has wide market prospect. The main active component of the essential oil of the leaves of cinnamomum japonicum is cineole which has the functions of removing dirt, relieving itching, reducing swelling and relieving pain. In addition, the essential oil of the cinnamomum japonicum leaves also contains natural spice components such as camphene, pinene and the like, so that the product can bring natural herbal medicine fragrance, and brings joyful feeling while refreshing.

The components have synergistic effect, so that the sterilization and bacteriostasis effects of the composition are effectively improved, and the Hongya camphor leaf essential oil and the borneol camphor leaf essential oil are mild in nature, free of stimulation, not easy to generate toxic and side effects, and wide in application range. In addition, the antibacterial no-clean gel has the conventional antibacterial function, and also has the effects of relieving red and swollen skin and skin itching and pain caused by mosquito bites.

In one embodiment, the mass ratio of the essential oil of the cinnamomum hongeucalypti camphor leaves to the essential oil of the cinnamomum camphora leaves is 1 (0.8-1.2).

In one embodiment, the bacteriostatic composition further comprises a second component, wherein the second component comprises a bamboo leaf extract and a lonicera confusa extract, and the mass ratio of the bamboo leaf extract to the lonicera confusa extract is 1 (0.5-2).

In one embodiment, the mass ratio of the bamboo leaf extract to the lonicera confusa extract is 1 (0.8-1.2).

The flos lonicerae extract has obvious bacteriostatic activity, and researches show that the flos lonicerae has inhibitory effects on typhoid bacillus, paratyphoid bacillus, escherichia coli, proteus, pseudomonas aeruginosa, bordetella pertussis, vibrio cholerae, staphylococcus, streptococcus, diplococcus pneumoniae, meningococcus and the like.

The bamboo leaf extract contains flavone, phenolic acid, anthraquinone, lactone, polysaccharide, amino acid, trace elements and other substances, has strong antibacterial, antioxidant and antifatigue effects, particularly contains rich bamboo leaf flavone, and is a strong antibacterial, antiviral and deodorant substance. And toxicological tests carried out by Zhejiang medical science institutes show that the bamboo leaf extract is more than 10g/kg of body weight of mice after oral administration of LD50, and the bamboo leaf extract is practically nontoxic, and the detection results of micronucleus experiments, sperm malformation experiments and Ames experiments are negative. Acute toxicity tests of rats also show that the bamboo leaf extract is actually nontoxic; no obvious toxic reaction is seen in the feeding test of rats for 30 days by each dosage group, and the obtained unmanned dosage is 5.0g/kg of body weight, so that the safety is high.

By adding the bamboo leaf extract and the lonicera confusa extract into the antibacterial composition, the antibacterial effect of the antibacterial composition can be effectively improved, and meanwhile, the moistening and oxidation resistance of the composition can be enhanced, and the comprehensive performance of the composition is improved.

In one embodiment, the mass ratio of the first component to the second component is (1-4): 1.

Controlling the mixing ratio of the first component and the second component within the above range can provide the above bacteriostatic composition with a better therapeutic effect.

In one embodiment, the borneol camphor leaf essential oil contains 25-100% of d-borneol by mass; and/or

In the essential oil of the cinnamomum japonicum leaves, the mass percentage of eucalyptol is 45-50%.

A preparation method of a bacteriostatic composition comprises the following steps:

mixing the first component with the first component;

mixing the bamboo leaf extract and the lonicera confusa extract to obtain the second component;

and mixing the first component and the second component to prepare the bacteriostatic composition.

The antibacterial composition is applied to preparation of daily necessities.

The antibacterial composition has good bactericidal and antibacterial effects, is mild in property and free of irritation, and is suitable for preparing daily necessities, such as: hand sanitizer, wet tissues, shampoo, shower gel and the like.

An antibacterial wash-free gel comprises the antibacterial composition, auxiliary materials acceptable in daily necessities and solvents acceptable in daily necessities.

In one embodiment, in the antibacterial no-clean gel, the antibacterial composition accounts for 0.5-5% by mass, and the coagulation auxiliary material accounts for 1-10% by mass.

In one embodiment, the auxiliary material includes: one or more of a gel matrix, a humectant, an acid-base modifier and a pigment;

the gel matrix is carbomer, xanthan gum, sodium carboxymethylcellulose and polyvinyl pyrrolidine;

the humectant is one or more of glycerol and propylene glycol;

the acid-base regulator is one or more of triethanolamine, citric acid and sodium citrate.

In one embodiment, the gel matrix is carbomer, the humectant is a combination of propylene glycol and glycerin, the pH modifier is triethanolamine, the pigment is an edible pigment, and the solvent is a combination of water and ethanol;

in the antibacterial leave-on gel, the carbomer accounts for 0.2-1% by mass, the propylene glycol accounts for 0.5-2% by mass, the glycerol accounts for 0.5-2% by mass, the triethanolamine accounts for 0.05-0.1% by mass, the food pigment accounts for 0.05-0.2% by mass, the ethanol accounts for 50-75% by mass, and the water accounts for 19.7-49.7% by mass.

The preparation method of the antibacterial leave-on gel comprises the following steps:

and dissolving the antibacterial composition and the auxiliary materials in a solvent, and uniformly stirring to obtain the antibacterial no-wash gel.

In one embodiment, the preparation method of the antibacterial leave-on gel comprises the following steps:

adding the carbomer into the water, stirring for dissolving, adding the bamboo leaf extract, the lonicera confusa extract, the propylene glycol, the glycerol and the pigment, and stirring uniformly to obtain a first solution;

dissolving the essential oil of the cinnamomum hongcinerea leaves and the essential oil of the cinnamomum camphora leaves in ethanol to obtain a second solution;

and mixing the first solution and the second solution, adding the triethanolamine, and adjusting the pH value to 6.5-7.5 to prepare the antibacterial leave-on gel.

The method can prepare the gel with better physical properties by selecting a proper material adding sequence. The preparation method is simple and convenient, does not need special instruments and operation skills, and can be widely applied.

The antibacterial no-clean gel is applied to the preparation of hand washing liquid.

The antibacterial no-wash gel can effectively sterilize and inhibit bacteria, is mild and non-irritant, can be prepared into a hand sanitizer for daily use, has no toxic or side effect, does not need to be cleaned, and is convenient to carry about.

Detailed Description

In order that the invention may be more fully understood, a more particular description of the invention will now be rendered by reference to specific embodiments thereof that are illustrated in the appended drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Example 1

Raw materials: 1% of Hongya camphor leaf essential oil, 1% of borneol camphor leaf essential oil, 0.5% of carbomer, 1% of propylene glycol, 1% of glycerol, 0.08% of triethanolamine, 0.1% of edible pigment, 60% of ethanol and the balance of water.

The preparation method comprises the following steps:

adding carbomer into the water, stirring for swelling and dispersing, adding folium Bambusae extract, flos Lonicerae extract, propylene glycol, glycerol and pigment, and stirring to obtain a first solution;

dissolving the essential oil of the leaves of the cinnamomum hongkongense and the essential oil of the leaves of the borneol cinnamomum camphora in ethanol to obtain a second solution;

and mixing the first solution and the second solution, adding triethanolamine to adjust the pH value to 6-7, and preparing the bacteriostatic no-wash gel of the example 1.

Example 2

Raw materials: 1% of Hongya camphor leaf essential oil, 1% of borneol camphor leaf essential oil, 0.1% of bamboo leaf extract, 0.1% of flos lonicerae extract, 0.5% of carbomer, 1% of propylene glycol, 1% of glycerol, 0.08% of triethanolamine, 0.1% of edible pigment, 60% of ethanol and the balance of water.

The preparation method comprises the following steps:

and mixing the first solution and the second solution, adding triethanolamine to adjust the pH value to 6-7, and preparing the bacteriostatic no-wash gel of the example 2.

Example 3

Raw materials: 2% of Hongya camphor leaf essential oil, 1% of borneol camphor leaf essential oil, 0.2% of bamboo leaf extract, 0.1% of flos lonicerae extract, 0.5% of carbomer, 1% of propylene glycol, 1% of glycerol, 0.08% of triethanolamine, 0.1% of edible pigment, 60% of ethanol and the balance of water.

The preparation method comprises the following steps:

and mixing the first solution and the second solution, adding triethanolamine to adjust the pH value to 6-7, and preparing the bacteriostatic no-wash gel of the example 3.

Example 4

Raw materials: 2% of Hongya camphor leaf essential oil, 1.5% of borneol camphor leaf essential oil, 0.2% of bamboo leaf extract, 0.5% of flos lonicerae extract, 0.5% of carbomer, 1% of propylene glycol, 1% of glycerol, 0.08% of triethanolamine, 0.1% of edible pigment, 60% of ethanol and the balance of water.

The preparation method comprises the following steps:

and mixing the first solution and the second solution, adding triethanolamine to adjust the pH value to 6-7, and preparing the bacteriostatic no-wash gel of the example 4.

Comparative example 1

2% of cinnamomum japonicum leaf essential oil, 0.1% of bamboo leaf extract, 0.1% of lonicera confusa extract, 0.5% of carbomer, 1% of propylene glycol, 1% of glycerol, 0.08% of triethanolamine, 0.1% of edible pigment, 60% of ethanol and the balance of water.

The preparation method comprises the following steps:

Comparative example 2

2% of borneol camphor leaf essential oil, 0.1% of bamboo leaf extract, 0.1% of flos lonicerae extract, 0.5% of carbomer, 1% of propylene glycol, 1% of glycerol, 0.08% of triethanolamine, 0.1% of edible pigment, 60% of ethanol and the balance of water.

The preparation method comprises the following steps:

Comparative example 3

Essentially the same as in example 2, except that only auxiliary materials were included, i.e.

Raw materials: carbomer 0.5%, propylene glycol 1%, glycerol 1%, triethanolamine 0.08%, food coloring agent 0.1%, ethanol 60%, and water in balance.

The preparation method comprises the following steps:

and adding carbomer into the mixed solution of water and ethanol, stirring for swelling and dispersing, adding propylene glycol, glycerol and pigment, stirring uniformly, adding triethanolamine, and adjusting the pH value to 6-7 to obtain the antibacterial leave-on gel of the comparative example 3.

Experiment for inhibiting bacteria

The wet tissues of examples 1-5 and comparative examples 1-4 were subjected to an antibacterial rate test with reference to appendix C of GB15979-2002 hygienic Standard for Disposable sanitary articles, test methods for sterilizing, bacteriostatic and stability of the products, and the action time was 5 minutes, and the results are shown in Table 1.

0.5 g of the gel was placed in a petri dish and 1X 10 was added⁸Shaking cfu/ml bacterial suspension and 49ml antibacterial water on an oscillator for 5, 15 and 30min, standing for 30min, taking 1ml of the treated bacterial suspension for 10-fold gradient dilution, taking 1ml of the treated bacterial suspension and a culture dish, adding a culture medium which is melted and cooled to about 40 ℃, shaking uniformly, culturing at 37 ℃ for 72h, and calculating the bacteriostasis rate, wherein the test results are shown in the following table 1:

TABLE 1

Wherein "-" means substantially no bactericidal effect.

As can be seen from table 1, the bacteriostatic compositions of examples 1-4 have strong bacteriostatic action. In addition, comparative example 1 is different from example 2 in that comparative example 1 does not contain the camphor leaf essential oil, and the amount of the camphor leaf essential oil of Hongya is twice that of example 1. Comparative example 2 compares to example 2 with the difference that comparative example 2 does not contain the essential oil of cinnamomum hongkongense leaf and the essential oil of cinnamomum camphora leaf is twice as much as example 2. As can be seen from Table 2, even if the concentration of the essential oil of Cinnamomum japonicum leaf or the essential oil of Cinnamomum camphora leaf is increased, the bacteriostatic effect is not as good

Example 2, it can be speculated that there may be a synergistic effect between essential oil of leaves of Cinnamomum japonicum and essential oil of leaves of Cinnamomum camphora.

In addition, the difference between example 1 and example 2 is that the bamboo leaf extract and the lonicera confusa extract are also added in example 2, and as can be seen from table 2, the bacteriostatic effect of example 2 is improved to a certain extent after the extracts are added.

Market research

Market research was conducted on 100 volunteers using the product of example 2, and the volunteers were 18-68 years old, male and female. The research project comprises the following steps: the cleaning effect, the moistening property, the irritation and the odor acceptability are respectively 10 points in each index score, four grades are respectively obtained, 10 points are very good, 6-9 points are very good, 2-5 points are generally obtained, and the difference is 1 point, and the statistical result is shown in the following table 2.

TABLE 2

As can be seen from Table 2, the leave-on gel of example 2 has better cleansing effect and moisturizing degree, less irritation and higher odor acceptance, and is suitable for wide application.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The bacteriostatic composition is characterized by comprising a first component and a second component, wherein the first component comprises essential oil of cinnamomum japonicum leaves and essential oil of cinnamomum camphora leaves, and the mass ratio of the essential oil of cinnamomum japonicum leaves to the essential oil of cinnamomum camphora leaves is 1 (0.8-1.2); in the borneol camphor leaf essential oil, the mass percentage of the d-borneol is 25-100%; in the essential oil of the cinnamomum japonicum leaves, the mass percentage content of eucalyptol is 45% -50%;

the second component consists of a bamboo leaf extract and a lonicera confusa extract, and the mass ratio of the bamboo leaf extract to the lonicera confusa extract is 1 (0.5-2);

the mass ratio of the first component to the second component is 10:1 or 5: 1.

2. The bacteriostatic composition according to claim 1, wherein the mass ratio of the bamboo leaf extract to the lonicera confusa extract is 1 (0.8-1.2).

3. Use of a bacteriostatic composition according to any one of claims 1-2 in the preparation of a commodity.

4. A bacteriostatic no-wash gel, which is characterized by consisting of the bacteriostatic composition as claimed in any one of claims 1 to 2, auxiliary materials acceptable in daily necessities and solvents acceptable in the daily necessities; the antibacterial composition comprises, by mass, 0.5% -5% of the antibacterial composition, 1% -10% of auxiliary materials and the balance of solvents.

5. The bacteriostatic leave-on gel according to claim 4, wherein the auxiliary materials comprise: one or more of a gel matrix, a humectant, an acid-base modifier and a pigment;

the gel matrix is one or more of carbomer, xanthan gum and sodium carboxymethylcellulose;

the humectant is one or more of glycerol and propylene glycol;

6. The bacteriostatic leave-on gel according to claim 5, wherein the gel matrix is carbomer, the humectant is a combination of propylene glycol and glycerin, the pH regulator is triethanolamine, the pigment is an edible pigment, and the solvent is a combination of water and ethanol;

7. A method for preparing a bacteriostatic leave-on gel according to any one of claims 4 to 6, which is characterized by comprising the following steps:

and dissolving the antibacterial composition and the auxiliary materials in the solvent, and uniformly stirring to obtain the antibacterial leave-on gel.