Tatjana V Kanjevac
University of Kragujevac, Faculty of Medical Sciences, Faculty Member
Chronic kidney disease (CKD) has become a worldwide public health problem. It is estimated that nearly 90% of patients with chronic renal failure manifest some symptoms of oral disease. With advances in medical treatment, CKD patients are... more
Chronic kidney disease (CKD) has become a worldwide public health problem. It is estimated that nearly 90% of patients with chronic renal failure manifest some symptoms of oral disease. With advances in medical treatment, CKD patients are living longer and require oral care. Chronic kidney disease is often accompanied by disturbances in mineral metabolism which are classified as their own clinical entity known as CKD-mineral and bone disorder (CKD-MBD). CKD-MBD affects all aspects of bone physiology: bone volume, bone turnover and bone mineralization. Jaw bones affected with CKD-MBD can have important clinical implications for the survival and osseointegration of dental implants, success of bone regeneration therapy, and increased risk of bone loss in patients with periodontitis or risk of bone fracture. Assessment of bone turnover is the most important diagnostic tool to monitor progression of CKD-MBD. Bone biomarkers and radiographical examination of bone density may be particularly useful to both, diagnosis and monitoring of bone turnover. Cone beam computed tomography (CBCT) as a reliable method with many advantages over other radiographic methods can be used for analysis of jaw bone micro-architectural changes and may be of a great help in bone quality determination in CKD-MBD.
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Research Interests: Radiology, Cancer, Cancer Biology, Medicine, Biopsy, and 3 moreParotid Gland, Pathological, and Malignancy
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Introduction. The aim of this study was to establish the number of roots and present the root canal configuration in the maxillary and mandibular premolar teeth, and evaluate the relations among these characteristics with gender and teeth... more
Introduction. The aim of this study was to establish the number of roots and present the root canal configuration in the maxillary and mandibular premolar teeth, and evaluate the relations among these characteristics with gender and teeth position in the Serbian population using cone-beam computed tomography. Material and Methods. Cone-beam computed tomography images of 570 teeth of 150 patients were evaluated. Teeth were classified into the following groups: maxillary first premolars, maxillary second premolars, mandibular first premolars and mandibular second premolars, and the number of roots and root canals per tooth, whereas root canal configurations were examined along with the tooth position and patients? gender. The root canal configuration was classified using Vertucci?s classification. Statistical significance was obtained using Chi square test. Results. In maxillary first premolars, two roots (53.5%) and two root canals (84.5%) were the most prevalent, as well as type IV ...
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The design of platinum based drugs is not a new field of interest. Platinum complexes are widely used as anticancer agents and currently, approximately 30 platinum(II) and platinum(IV) entered into some of the phases of clinical trials. A... more
The design of platinum based drugs is not a new field of interest. Platinum complexes are widely used as anticancer agents and currently, approximately 30 platinum(II) and platinum(IV) entered into some of the phases of clinical trials. A special place in today’s research belongs to platinum complexes with diammine ligands. A large number of edda (ethylenediamine- N, N’-diacetate)-type ligands and their corresponding metal complexes has been successfully synthesized. This article summarizes recent progress in research on edda-type-platinum complexes. Some of these agents achieves better effect compared to the gold standard (cisplatin). It has been shown that there is a possible relationship between the length of the ligand ester group carbon chain and its cytotoxic effect. In most cases the longer the ester chain is the greater is the antitumor activity. Of particular interest are the noticeable effects of some new platinum compound with edda-type ligand on cell lines that are known...
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The study of teeth is of great interest to anthropologists, biologists, orthodontists and forensic scientists. The existence of sexual dimorphism in permanent teeth is a known phenomenon. Aim of this study was to analyze the presence of... more
The study of teeth is of great interest to anthropologists, biologists, orthodontists and forensic scientists. The existence of sexual dimorphism in permanent teeth is a known phenomenon. Aim of this study was to analyze the presence of sexual dimorphism in the mesiodistal and vestibulolingual diameter of permanent teeth in the sample of Serbian population. Measurements were taken on plaster casts of 201 individuals of both sexes, ages between 18-25 years, using a digital caliper with 0.01 mm precision. The mesiodistal and vestibulolingual diameter of each permanent tooth was determined. A Student's t-test and a Mann-Whitney U test were used to statistically analyze the obtained results. There were no statistically significant differences in the teeth crown diameter between the right and left side of the same dental arch. Majority of the teeth examined were larger in male than in female patients. Statistically significant difference in the mesiodistal diameter of male and female maxillary and mandibular canines was found. The results of this study indicate that there are significant differences in teeth size between sexes in Serbian population. Males have larger diameters in teeth crowns than females. Canines show the greatest dimorphism.
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We have studied the kinetics of the complex formation of gold(III) complexes, [Au(en)Cl2]+ (dichlorido( ethylendiamine)aurate(III)-ion) [Au(dach)Cl2] (dichloride(1,2-diaminocyclohexane)aurate(III)-ion) and [Au(bipy)Cl2]+... more
We have studied the kinetics of the complex formation of gold(III) complexes, [Au(en)Cl2]+ (dichlorido( ethylendiamine)aurate(III)-ion) [Au(dach)Cl2] (dichloride(1,2-diaminocyclohexane)aurate(III)-ion) and [Au(bipy)Cl2]+ (dichlorido(2,2'-bipyridyl)aurate(III)-ion) with guanosine5'-monophosphate (5'-GMP). It was shown that 5'-GMP have a high affinity for gold(III) complex, which may have important biological implications, since the interactions of Au(III) with DNA are thought to be responsible for the anti-tumor activity. The [Au(bipy)Cl2]+ complex is more reactive than [Au(en)Cl2]+ or [Au(dach)Cl2]+. The activation parameters for all studied reactions suggest an associative substitution mechanism. The cytotoxicity of gold(III) complexes was tested on A549 human lung carcinoma epithelial cell line and was evaluated by cytotoxic (MTT and LDH test) and apoptotic assays. The results showed that all tested gold(III) complexes displayed cytotoxic effect on A549 cells. Among the tested gold (III) complexes, AuBIPY showed the best cytotoxic effects.
Research Interests: Chemistry, Medicinal Chemistry, Cytotoxicity, Apoptosis, Medicine, and 10 moreCell Culture, Humans, Oral Squamous Cell Carcinoma (OSCC), Epithelial cells, Cell Proliferation, Structure activity Relationship, Cell Survival, Antineoplastic Agents, Pharmacology and pharmaceutical sciences, and lung neoplasms
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The aim of restorative dentistry is the successful restoration and maintenance of the health of the tooth by an adequate reconstructive treatment to re-establish the function of damaged pulp. The potential cytotoxicity of specific... more
The aim of restorative dentistry is the successful restoration and maintenance of the health of the tooth by an adequate reconstructive treatment to re-establish the function of damaged pulp. The potential cytotoxicity of specific materials used in restorative dentistry has been widely studied, and the aim of this review article is to summaries and discuss the cytotoxicity of glass ionomer cements when they are in direct or indirect contact with the pulp tissue. Resin modified and metal reinforced glass ionomer cements, in comparison to conventional glass ionomer cements, showed higher cytotoxic effects on pulp cells in vitro. In vivo, the dentin barrier between toxic glass ionomer cements and the pulp cells may prevent pulp cell damage. Potentially toxic resin modified and metal reinforced glass ionomer cements should not be applied directly to the pulp tissue.
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Bone quality is an important factor that may determine implant success. In addition to the other parameters that define bone quality, vascularity plays a vital role in the process of osseointegration of dental implants. Laser Doppler... more
Bone quality is an important factor that may determine implant success. In addition to the other parameters that define bone quality, vascularity plays a vital role in the process of osseointegration of dental implants. Laser Doppler flowmetry (LDF) is an appropriate method for assessment of tissue vascularity at the level of microcirculation. It is non-invasive, painless and well accepted by the patients. The results are expressed in Perfusion Units (PU). The aim of this study was to estimate bone vascularity in the human posterior maxilla during implant insertion using LDF. Nine patients, three females and six males, mean age 57.56 ± 8.83 years, consecutively treated with 54 implants were enrolled in this study. Implants were inserted in the posterior maxilla, in positions of first premolar, second premolar and first molar bilaterally in each patient. After implant site preparation with a drill of diameter 2.8 mm, bone vascularity was measured using LDF. Mean LDF value for 54 oste...
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The aim of restorative dentistry is the successful restoration and maintenance of the health of the tooth by an adequate reconstructive treatment to re-establish the function of damaged pulp. The potential cytotoxicity of specific... more
The aim of restorative dentistry is the successful restoration and maintenance of the health of the tooth by an adequate reconstructive treatment to re-establish the function of damaged pulp. The potential cytotoxicity of specific materials used in restorative dentistry has been widely studied, and the aim of this review article is to summarise and discuss the cytotoxicity of glass ionomer cements when they are in direct or indirect contact with the pulp tissue. Resin modified and metal reinforced glass ionomer cements, in comparison to conventional glass ionomer cements, showed higher cytotoxic effects on pulp cells in vitro. In vivo, the dentin barrier between toxic glass ionomer cements and the pulp cells may prevent pulp cell damage. Potentially toxic resin modified and metal reinforced glass ionomer cements should not be applied directly to the pulp tissue.
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Introduction: Accessory mental foramen (AMF) is defined as any additional opening on the anterior surface of the mandible body that is connected to the mandibular canal. The presence of AMF is an important anatomical parameter when... more
Introduction: Accessory mental foramen (AMF) is defined as any additional opening on the anterior surface of the mandible body that is connected to the mandibular canal. The presence of AMF is an important anatomical parameter when planning the therapy to avoid neurovascular bundle injury and other complications. Cone-beam computed tomography (CBCT) provides an accurate, three-dimensional determining of the position, its dimensions and the relation of AMF to the mental foramen (MF), as well as the distinction from nutritive openings. Material and Methods: The research was carried out at the Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, as a retrospective study where 148 CBCT images were analyzed. The analysis of the position of AMF, the relation to and the distance from the MF were made on cross-sectional and axial images. Only those openings that have had a clear connection with the mandibular canal were counted as AMF. Results: AMF was present in 12 (8.11%) patients. In most cases, AMF was positioned superior to MF, in 27% of patients. There was no significant statistical difference between sex and the jaw side. The average distance of AMF from MF was 4.52 ± 2.21 mm. In most cases, AMF is round shaped (60%). The average value of the surface area is 1.62 ± 1.14 mm2. Discussion and Conclusion: Timely detection of AMF using CBCT contributes to the diagnosis and planning of appropriate dentures, surgical technique, preventing possible damage to adjacent anatomical structures, or some other therapy.
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Background: We aimed to determine the socio-economic factors associated with unmet healthcare needs of the population aged 20 and over in Serbia. Methods: We used data from the 2013 National Health Survey (NHS) of the population of... more
Background: We aimed to determine the socio-economic factors associated with unmet healthcare needs of the population aged 20 and over in Serbia. Methods: We used data from the 2013 National Health Survey (NHS) of the population of Serbia. We focused only on the data concerning the population aged 20 and over. The final sample thus included 13,765 participants. The logistic regression was used to examine the socio-economic factors associated with unmet health care needs. Results: According to the data obtained in this study, 26.2% of the population aged 20 and over reported unmet health care needs during the previous 12 months. The multivariate analysis shows that significant indicators of unmet healthcare needs include: gender, age, marital status, level of education, financial and employment status. Conclusion: Females, the elderly and those with the lowest levels of education and household income, as well as those who are divorced and unemployed are at highest risk of unmet healt...
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Aim: We investigated the antitumor effects of zinc(II) complex with S-propyl thiosalicylic acid [Zn( S-pr-thiosal)2] in 4T1 murine breast cancer model. Results: The Zn( S-pr-thiosal)2 complex reduced primary tumor growth in vivo and... more
Aim: We investigated the antitumor effects of zinc(II) complex with S-propyl thiosalicylic acid [Zn( S-pr-thiosal)2] in 4T1 murine breast cancer model. Results: The Zn( S-pr-thiosal)2 complex reduced primary tumor growth in vivo and induced tumor cell apoptosis. The Zn( S-pr-thiosal)2 complex disrupted the balance between pro- and antiapoptotic Bcl-2 family members in 4T1 cells and induced G1/S cell cycle arrest. The Zn( S-pr-thiosal)2 complex increased the percentage of p16, p21 and p27 positive 4T1 cells. There was a significantly decrease in expression of STAT3 and its targets c-Myc and cyclin D3 in 4T1 cells treated with the Zn( S-pr-thiosal)2 complex thus contributing to G1/S cell cycle arrest and/or apoptosis. Conclusion: Our data suggest that the Zn( S-pr-thiosal)2 complex restricted tumor growth through induction of mitochondrial-driven apoptosis and suppression of cell cycle progression.
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Introduction/Objective. Serbia has been burdened with one of the highest cervical cancer incidence and mortality rates in Europe. The objective of the study was to estimate the prevalence and factors associated with compliance to cervical... more
Introduction/Objective. Serbia has been burdened with one of the highest cervical cancer incidence and mortality rates in Europe. The objective of the study was to estimate the prevalence and factors associated with compliance to cervical cancer screening among women in the general population. Methods. The study used the data from 2013 National Health Survey of the population of Serbia. Logistic regression analysis was further used to examine demographic and socio-economic factors which affect the disparities in cervical cancer screening practices among the female population. Results. Every third woman (35.4%) has never done a Pap test in her lifetime. The highest percentage of respondents did their Pap tests after they were recommended by doctors (52.3%); 45% of women did it on their own initiative, and only 2.7% did it after they had been summoned to participate in an organized screening by their doctor. The multivariate logistic regression analysis revealed that the most importan...
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Introduction/Objective. This study was aimed at evaluating dentin permeability after irrigation with sodium hypochlorite (NaOCl) and final rinse with chlorhexidine (CHX), ethylenediamine tetraacetic acid (EDTA) + CHX, and new combination... more
Introduction/Objective. This study was aimed at evaluating dentin permeability after irrigation with sodium hypochlorite (NaOCl) and final rinse with chlorhexidine (CHX), ethylenediamine tetraacetic acid (EDTA) + CHX, and new combination products: QMiX or MTAD. Methods. Roots of 60 maxillary incisors were randomly divided into five groups (n = 12) before instrumentation and irrigation with NaOCl according to the final irrigation regimen: CHX (2% CHX), EDTA + CHX (17% EDTA + 2% CHX), QMiX, MTAD, and control group (distilled water). After final irrigation, ten roots of each group were horizontally sectioned and dye penetration was evaluated in the coronal, middle, and apical thirds. Remaining samples were subjected to scanning electron microscopy. Data were analyzed with ANOVA/Tukey?s test. Results. Less dye penetration was found in CHX group compared with control as well as with QMiX and MTAD group in all thirds (p < 0.05). A significant difference between the control and EDTA + C...
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Introduction. The aim of this study was to translate the Child Oral Impact on Daily Performance index into Serbian language and to evaluate its reliability in practice. Material and Methods. Following internationally established methods,... more
Introduction. The aim of this study was to translate the Child Oral Impact on Daily Performance index into Serbian language and to evaluate its reliability in practice. Material and Methods. Following internationally established methods, adaptation of the Child Oral Impact on Daily Performance index for children and adolescents in the Serbian speaking areas consisted of three steps: forward translation of the Child Oral Impact on Daily Performance index, backward translation, and a pilot study. Results. A pilot study included 42 participants (21 males and 21 females), mean age of 12.0 ? 1.01 years. All items showed a corrected item-total correlation coefficient above >0.20 and the standardized Cronbach?s alpha coefficient was 0.80. All correlation coefficient values were positive. The prevalence of oral health impact measured by the Child Oral Impact on Daily Performance index was relatively high and 54.8% of the participants reported at least one oral impact on the daily perform...
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Ulcerative colitis (UC) is a complex disease in which the interaction of genetic, environmental, and microbial factors drives chronic intestinal inflammation. Gastrointestinal symptoms are predominant including pathological manifestations... more
Ulcerative colitis (UC) is a complex disease in which the interaction of genetic, environmental, and microbial factors drives chronic intestinal inflammation. Gastrointestinal symptoms are predominant including pathological manifestations in the oral cavity, as well as extra-intestinal complications. Lingua plicata (LP) is a condition characterized by an increased number of fissures and grooves at the central and lateral aspects of the dorsal surface of the tongue. LP is usually asymptomatic and discovered incidentally, but the accumulation of food in the fissures and grooves can lead to focal glossitis and halitosis. In this study, we analyzed the level of proinflammatory cytokines in gingival crevicular fluid (GCF) and demonstrated that proinflammatory cytokines IFN-γ, IL-12, and IL-1β were significantly increased in the group of UC patients when compared with healthy controls. Obtained results have shown that 35% of UC patients have lingua plicata. The GCF levels of IFN-γ and IL-...
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Ulcerative colitis (UC) is a complex disease in which the interaction of genetic, environmental, and microbial factors drives chronic intestinal inflammation. Gastrointestinal symptoms are predominant including pathological manifestations... more
Ulcerative colitis (UC) is a complex disease in which the interaction of genetic, environmental, and microbial factors drives chronic intestinal inflammation. Gastrointestinal symptoms are predominant including pathological manifestations in the oral cavity, as well as extra-intestinal complications. Lingua plicata (LP) is a condition characterized by an increased number of fissures and grooves at the central and lateral aspects of the dorsal surface of the tongue. LP is usually asymptomatic and discovered incidentally, but the accumulation of food in the fissures and grooves can lead to focal glossitis and halitosis. In this study, we analyzed the level of proinflammatory cytokines in gingival crevicular fluid (GCF) and demonstrated that proinflammatory cytokines IFN-γ, IL-12, and IL-1β were significantly increased in the group of UC patients when compared with healthy controls. Obtained results have shown that 35% of UC patients have lingua plicata. The GCF levels of IFN-γ and IL-12 were higher in UC patients with LP compared with UC patients without lingua plicata. In conclusion, increased GCF values of IFN-γ and IL-12, in UC patients with LP may be considered as a sign of the disease progression and, consequently, of a poor prognosis for patients.
Dental caries and periodontitis are among the most common health conditions that are currently recognized as growing socio-economic problems relating to their increasing prevalence, negative socio-economic impact, and harmful effects on... more
Dental caries and periodontitis are among the most common health conditions that are currently recognized as growing socio-economic problems relating to their increasing prevalence, negative socio-economic impact, and harmful effects on systemic health. So far, the exact effects of caries and standard restorative materials on periodontal inflammatory and oxidative status are not established. The present study aimed to investigate the effect of caries and its restoration using standard temporary and permanent filling materials on a panel of 16 inflammatory and oxidative markers in gingival crevicular fluid (GCF) of periodontally healthy individuals, 7 (D7) and 30 (D30) days post-restoration, while the intact teeth represented the control. One hundred ninety systemically and periodontally healthy patients with occlusal caries underwent standard cavity preparation and restorations with one of six standard temporary or permanent restorative material according to indication and randomiza...
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Background/Aim. In the spirit of personalized medicine, determining caries biomarkers in the saliva and gingival crevicular fluid (GCF) attracts great attention in the current dental re-search. The concentration of GCF cytokines is... more
Background/Aim. In the spirit of personalized medicine, determining caries biomarkers in the saliva and gingival crevicular fluid (GCF) attracts great attention in the current dental re-search. The concentration of GCF cytokines is illustrative in depicting the processes in tooth structures. Their relevance must be inspected with aspects of tooth position and caries lesion level. Different impacts of dental restoration materials on GCF IL-1? and TNF-? could be used as a parameter for estimating local inflammation. This paper aimed to estimate the concentrations of the proinflammatory cytokines (IL-1? and TNF-?) in the GCF and to correlate them with caries extension, tooth position, and different restorative biomaterials. Methods. GCF samples were collected from 90 periodontally healthy patients demonstrating at least one tooth with proximal caries and one intact tooth, at the baseline, 7 and 30-days post-treatment. The biomarkers? profile was investigated in relation to different le...
Research Interests: Oncology, Biomedical Engineering, Drug delivery, Cancer Research, Medicine, and 13 moreMesenchymal stem cells, Tumor microenvironment, Humans, Cancer Therapy, Animals, Oncolytic Viruses, Drug Delivery Systems, Medical Pharmacology, Antineoplastic Agents, Cellular Therapy, Mesenchymal Stromal Cells, Biochemistry and cell biology, and Target Therapy
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We have studied the kinetics of the complex formation of gold(III) complexes, [Au(en)Cl2]+ (dichlorido( ethylendiamine)aurate(III)-ion) [Au(dach)Cl2] (dichloride(1,2-diaminocyclohexane)aurate(III)-ion) and [Au(bipy)Cl2]+... more
We have studied the kinetics of the complex formation of gold(III) complexes, [Au(en)Cl2]+ (dichlorido( ethylendiamine)aurate(III)-ion) [Au(dach)Cl2] (dichloride(1,2-diaminocyclohexane)aurate(III)-ion) and [Au(bipy)Cl2]+ (dichlorido(2,2&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-bipyridyl)aurate(III)-ion) with guanosine5&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-monophosphate (5&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-GMP). It was shown that 5&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-GMP have a high affinity for gold(III) complex, which may have important biological implications, since the interactions of Au(III) with DNA are thought to be responsible for the anti-tumor activity. The [Au(bipy)Cl2]+ complex is more reactive than [Au(en)Cl2]+ or [Au(dach)Cl2]+. The activation parameters for all studied reactions suggest an associative substitution mechanism. The cytotoxicity of gold(III) complexes was tested on A549 human lung carcinoma epithelial cell line and was evaluated by cytotoxic (MTT and LDH test) and apoptotic assays. The results showed that all tested gold(III) complexes displayed cytotoxic effect on A549 cells. Among the tested gold (III) complexes, AuBIPY showed the best cytotoxic effects.