In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and... more
In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Research Interests:
Research Interests: Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Bolivia, Ethnopharmacology, and 17 moreLipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system(Lipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system)
(Lipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system)
Research Interests:
Research Interests:
In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and... more
In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Research Interests:
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2),... more
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Research Interests:
Research Interests: Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Bolivia, Ethnopharmacology, and 17 moreLipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system(Lipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system)
(Lipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system)
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5,... more
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5, 7-dihydroxy-3, 4'-dimethoxyflavone (ermanin)(2), were isolated.
Research Interests:
In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and... more
In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Research Interests:
Research Interests:
Research Interests:
Research Interests: Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Bolivia, Ethnopharmacology, and 17 moreLipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system(Lipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system)
(Lipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system)
Research Interests:
Research Interests:
Research Interests: Analytical Chemistry, Organic Chemistry, Pharmacy, Complementary and Alternative Medicine, Traditional Medicine, and 45 morePlant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves(Plant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves)
(Plant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves)
In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and... more
In the course of our search for plant natural products as antiviral agents, extracts of ten plants from the Iberian Peninsula were tested for antiviral activity against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Aqueous extracts of five of these medicinal plants, namely Nepeta nepetella (150-500 microg/mL), Nepeta coerulea (150-500 microg/mL), Nepeta tuberosa (150-500 microg/mL), Dittrichia viscosa (50-125 microg/mL) and Sanguisorba minor magnolii (50-125 microg/mL), showed a clear antiviral activity against two different DNA and RNA viruses, i.e. HSV-1 and VSV. Only the medicinal plant Dittrichia viscosa was active against an additional virus, poliovirus type 1.
Research Interests:
Research Interests:
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2),... more
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Research Interests:
Research Interests: Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Bolivia, Ethnopharmacology, and 17 moreLipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system(Lipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system)
(Lipopolysaccharide, Mice, Nitric oxide, Animals, Male, South America, Anti-inflammatory agents, lipoxygenase (LOX) inhibitors, Plant extracts, Tumor necrosis factor-alpha, Anti Inflammatory Activity, Mouse Peritoneal-Macrophages, Cell Survival, Aqueous Extract, Prostaglandin E2, Baccharis, and Cellular system)
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2),... more
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Research Interests:
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5,... more
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5, 7-dihydroxy-3, 4'-dimethoxyflavone (ermanin)(2), were isolated.
Research Interests:
Research Interests:
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5,... more
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5, 7-dihydroxy-3, 4'-dimethoxyflavone (ermanin)(2), were isolated.
Research Interests:
Research Interests:
Research Interests:
As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro... more
As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Five of these compounds showed antiviral activity against VSV. The percentages of cellular viability at the non-toxic limit concentrations of the active compounds were: verbascoside 53.6 % at 500 microg/ml, 8-acetylharpagide 32.1 % at 500 microg/ml, harpagoside 43.3 % at 450 microg/ml, scorodioside 47.8 % at 500 microg/ml and buddlejasaponin IV 56.9 % at 25 microg/ml. Although none of the saikosaponins were active against HSV-1, the iridoid scorodioside showed moderate in vitro anti-HSV-1 activity (30.6 % at 500 microg/ml). However, none of the compounds tested in this survey had any effect against poliovirus.
Research Interests: Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Saponins, Cercopithecus aethiops, and 17 moreCell line, Humans, Animals, Bupleurum, Phenols, Plant extracts, HeLa cells, Iridoids, Antiviral Activity, Vero cells, Planta, Scrophulariaceae, Antiviral Agents, Oleanolic acid, Poliovirus, Carbohydrate Sequence, and Molecular Sequence Data
Research Interests:
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2),... more
Four coumarins were isolated from the EtOAc extract of the flower-tops of Santolina oblongifolia Boiss. (Compositae). They were identified as 7-methoxycoumarin (herniarin) (1), 6,7-dihydroxycoumarin (aesculetin) (2), 6-methoxy-7-glucosidylcoumarin (scopolin) (3), and 6-hydroxy-7-methoxycoumarin (scopoletin) (4). This is the first report of the isolation of aesculetin and scopolin from the genus Santolina. The isolated coumarins showed marked activity as inhibitors of eicosanoid-release from ionophore-stimulated mouse peritoneal macrophages.
Research Interests:
Research Interests: Analytical Chemistry, Organic Chemistry, Pharmacy, Complementary and Alternative Medicine, Traditional Medicine, and 45 morePlant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves(Plant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves)
(Plant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves)
The CH2Cl2 extract of Tanacetum microphyllum aerial parts exhibited anti-inflammatory activity and yielded two anti-inflammatory flavonoids: 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone (centaureidin) [1], and... more
The CH2Cl2 extract of Tanacetum microphyllum aerial parts exhibited anti-inflammatory activity and yielded two anti-inflammatory flavonoids: 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone (centaureidin) [1], and 5,3'-dihydroxy-4'-methoxy-7-carbomethoxyflavonol [2]. This is the first report of the chemical composition of Ta. microphyllum and of the isolation of centaureidin from the genus Tanacetum.
Research Interests:
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5,... more
The CH2Cl2 extract of Tanacetum microphyllum exhibited antiinflammatory activity on PMA-mouse ear model. Two antiinflammatory flavonoids, 5, 7-dihydroxy-3, 6, 4'-trimethoxyflavone (santin)(1) and 5, 7-dihydroxy-3, 4'-dimethoxyflavone (ermanin)(2), were isolated.
Research Interests:
As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro... more
As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Five of these compounds showed antiviral activity against VSV. The percentages of cellular viability at the non-toxic limit concentrations of the active compounds were: verbascoside 53.6 % at 500 microg/ml, 8-acetylharpagide 32.1 % at 500 microg/ml, harpagoside 43.3 % at 450 microg/ml, scorodioside 47.8 % at 500 microg/ml and buddlejasaponin IV 56.9 % at 25 microg/ml. Although none of the saikosaponins were active against HSV-1, the iridoid scorodioside showed moderate in vitro anti-HSV-1 activity (30.6 % at 500 microg/ml). However, none of the compounds tested in this survey had any effect against poliovirus.
Research Interests: Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Saponins, Cercopithecus aethiops, and 17 moreCell line, Humans, Animals, Bupleurum, Phenols, Plant extracts, HeLa cells, Iridoids, Antiviral Activity, Vero cells, Planta, Scrophulariaceae, Antiviral Agents, Oleanolic acid, Poliovirus, Carbohydrate Sequence, and Molecular Sequence Data
Research Interests:
Research Interests:
Research Interests: Analytical Chemistry, Organic Chemistry, Pharmacy, Complementary and Alternative Medicine, Traditional Medicine, and 45 morePlant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves(Plant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves)
(Plant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves)
Research Interests:
The CH2Cl2 extract of Tanacetum microphyllum aerial parts exhibited anti-inflammatory activity and yielded two anti-inflammatory flavonoids: 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone (centaureidin) [1], and... more
The CH2Cl2 extract of Tanacetum microphyllum aerial parts exhibited anti-inflammatory activity and yielded two anti-inflammatory flavonoids: 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone (centaureidin) [1], and 5,3'-dihydroxy-4'-methoxy-7-carbomethoxyflavonol [2]. This is the first report of the chemical composition of Ta. microphyllum and of the isolation of centaureidin from the genus Tanacetum.
Research Interests:
As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro... more
As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Five of these compounds showed antiviral activity against VSV. The percentages of cellular viability at the non-toxic limit concentrations of the active compounds were: verbascoside 53.6 % at 500 microg/ml, 8-acetylharpagide 32.1 % at 500 microg/ml, harpagoside 43.3 % at 450 microg/ml, scorodioside 47.8 % at 500 microg/ml and buddlejasaponin IV 56.9 % at 25 microg/ml. Although none of the saikosaponins were active against HSV-1, the iridoid scorodioside showed moderate in vitro anti-HSV-1 activity (30.6 % at 500 microg/ml). However, none of the compounds tested in this survey had any effect against poliovirus.
Research Interests: Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Saponins, Cercopithecus aethiops, and 17 moreCell line, Humans, Animals, Bupleurum, Phenols, Plant extracts, HeLa cells, Iridoids, Antiviral Activity, Vero cells, Planta, Scrophulariaceae, Antiviral Agents, Oleanolic acid, Poliovirus, Carbohydrate Sequence, and Molecular Sequence Data
Research Interests: Analytical Chemistry, Organic Chemistry, Pharmacy, Complementary and Alternative Medicine, Traditional Medicine, and 45 morePlant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves(Plant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves)
(Plant Biology, Life Sciences, Ethnopharmacology, Medicinal Plants, Ecuador, Fluorescent Dyes and Reagents, Dendrimers, Pharmaceutics, Polyamines, Essential Oil, Antioxidants, Cell line, Reactive Oxygen Species, Mice, Nitric oxide, Female, Animals, Microglia, Natural Product, Male, Ethanol, Pharmacology and toxicology, Zinc, Ficus, Anti-inflammatory agents, Lipid peroxidation, Anions, Coumarins, Plant extracts, Soybeans, Rats, Edema, Tumor necrosis factor-alpha, Wistar Rats, Carrageenan, Food Sciences, N-Acetylcysteine, Structure activity Relationship, Cell Survival, Superoxides, Xanthine Oxidase, Biochemistry and cell biology, Molecular Structure, Indomethacin, and Plant Leaves)
Research Interests:
Research Interests:
The CH2Cl2 extract of Tanacetum microphyllum aerial parts exhibited anti-inflammatory activity and yielded two anti-inflammatory flavonoids: 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone (centaureidin) [1], and... more
The CH2Cl2 extract of Tanacetum microphyllum aerial parts exhibited anti-inflammatory activity and yielded two anti-inflammatory flavonoids: 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone (centaureidin) [1], and 5,3'-dihydroxy-4'-methoxy-7-carbomethoxyflavonol [2]. This is the first report of the chemical composition of Ta. microphyllum and of the isolation of centaureidin from the genus Tanacetum.
Research Interests:
As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro... more
As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Five of these compounds showed antiviral activity against VSV. The percentages of cellular viability at the non-toxic limit concentrations of the active compounds were: verbascoside 53.6 % at 500 microg/ml, 8-acetylharpagide 32.1 % at 500 microg/ml, harpagoside 43.3 % at 450 microg/ml, scorodioside 47.8 % at 500 microg/ml and buddlejasaponin IV 56.9 % at 25 microg/ml. Although none of the saikosaponins were active against HSV-1, the iridoid scorodioside showed moderate in vitro anti-HSV-1 activity (30.6 % at 500 microg/ml). However, none of the compounds tested in this survey had any effect against poliovirus.
Research Interests: Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Saponins, Cercopithecus aethiops, and 17 moreCell line, Humans, Animals, Bupleurum, Phenols, Plant extracts, HeLa cells, Iridoids, Antiviral Activity, Vero cells, Planta, Scrophulariaceae, Antiviral Agents, Oleanolic acid, Poliovirus, Carbohydrate Sequence, and Molecular Sequence Data
In the present study, the effects of compounds extracted from Tanacetum microphyllum, four flavonoids and one sesquiterpene lactone, on arachidonic acid metabolism in cellular systems were examined. In the PGE2- and LTC4-release assay,... more
In the present study, the effects of compounds extracted from Tanacetum microphyllum, four flavonoids and one sesquiterpene lactone, on arachidonic acid metabolism in cellular systems were examined. In the PGE2- and LTC4-release assay, most compounds did not exhibit any significant effect. Only hydroxyachillin, and the flavonoids centaureidin and 5,3'-dihydroxy-4'-methoxy-7-methoxycarbonylflavonol showed a significant effect, although with less potency than the reference drugs, indomethacin and NDGA. However, all compounds inhibited the release of TXB2 from ionophore-stimulated human platelets. The action is more marked with the flavonoids santin and ermanin.
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The CH2Cl2 extract of Tanacetum microphyllum aerial parts exhibited anti-inflammatory activity and yielded two anti-inflammatory flavonoids: 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone (centaureidin) [1], and... more
The CH2Cl2 extract of Tanacetum microphyllum aerial parts exhibited anti-inflammatory activity and yielded two anti-inflammatory flavonoids: 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone (centaureidin) [1], and 5,3'-dihydroxy-4'-methoxy-7-carbomethoxyflavonol [2]. This is the first report of the chemical composition of Ta. microphyllum and of the isolation of centaureidin from the genus Tanacetum.
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Research Interests:
Six diterpenes (three clerodanes, two abietanes and one rosane) were tested for interactions with the cyclooxygenase and 5-lipoxygenase pathways of arachidonate metabolism and for effects of nitric oxide production. Two abietane... more
Six diterpenes (three clerodanes, two abietanes and one rosane) were tested for interactions with the cyclooxygenase and 5-lipoxygenase pathways of arachidonate metabolism and for effects of nitric oxide production. Two abietane diterpenes, aethiopinone and 11,12-dihydroxy-6-oxo-8,11,13-abietatriene and the rosane lagascatriol showed a remarkable effect on COX-1 pathway of PGE2 release in calcium ionophore A23187-stimulated peritoneal macrophages. Only the two latter diterpenes showed inhibition on COX-2 pathway of PGE2 release in E. coli LPS-stimulated peritoneal macrophages. In addition, all compounds assayed were inhibitors of LTC4 release with IC50 < or = 10 microM. Clerodane diterpenes were inactive in COX assay. None of the diterpenes assayed, except 11,12-dihydroxy-6-oxo-8,11,13-abietatriene, affected NO production. The results obtained suggest that the cellular mechanisms of action of some of these substances may involve inhibition of cyclooxygenase/lipoxygenase pathways and nitric oxide production.