1.はじめに 一般に,液晶製造等のクリーンルームは,静電気が非 常に発生し易い環境となっており,ガラス基板の表面上 に 空 気 中 の 塵 埃 を 付 着 さ せ る 静 電 吸 着(ESA: Electrostatic Attraction)や,静電気放電(ESD:Electrostatic Discharge)による集積回路の絶縁破壊,および ESD で 発生した電磁干渉(EMI:Electromagnetic Interference)に... more
1.はじめに 一般に,液晶製造等のクリーンルームは,静電気が非 常に発生し易い環境となっており,ガラス基板の表面上 に 空 気 中 の 塵 埃 を 付 着 さ せ る 静 電 吸 着(ESA: Electrostatic Attraction)や,静電気放電(ESD:Electrostatic Discharge)による集積回路の絶縁破壊,および ESD で 発生した電磁干渉(EMI:Electromagnetic Interference)に よる生産装置の誤動作等の生産障害を引き起こし,製品 の歩留まり低下の問題となっている.これらの障害を防 ぐ静電気対策の一つとして,イオナイザーによる静電気 除去(除電)が挙げられ,コロナ放電方式,放射線方式 (軟 X 線方式),紫外線方式等によるものが知られている. 中でもコロナ放電式は他の方式に比べて安価で簡便なた め広く利用されている.しかし,このコロナ放電式のイ オナイザーは,クリーンルームの空気中に含まれる微量 電極加熱による低発塵コロナ放電式イオナイザーの開発 佐藤 朋且 ,鈴木 政典,水野 彰 (2013年5月8日受付;2013年7月22日受理) Development of Low-particle-emission Corona Discharge Ionizer by Electrode Heating Tomokatsu SATO , Masanori SUZUKI, Akira MIZUNO (Received May 8, 2013; Accepted July 22, 2013)
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SAVER-Net (South American Environmental Risk Management Network) is a trilateral international collaboration among Japan, Chile, and Argentina supported under the JST-JIICA SATREPS program. The major aim of the project is establishing... more
SAVER-Net (South American Environmental Risk Management Network) is a trilateral international collaboration among Japan, Chile, and Argentina supported under the JST-JIICA SATREPS program. The major aim of the project is establishing observation network of UV/ozone and aerosols over Chile and Argentina where was a blank area of measurement stations on a global scale. The project was finished this year after 5 years of implementation. Thirty-six pre-existent and newly installed UV radiometers distributed over Chile and Argentina were connected on-line. All UV observational data were compiled into a common information platform called GeoUV and risk information/alert is released to the registered users. Ozone monitoring capability at the southern end of the South American continent, i.e. at Rio Gallegos in Argentina and Punta Arenas in Chile, was consolidated to make a comprehensive study of ozone hole. A Differential Absorption Lidar (DIAL), a millimeter-wave spectrometer, Brewer spe...
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This paper is aimed to study the characteristics of the positive dc corona discharges in single wire-duct electrostatic precipitators. Therefore, the corona discharges were formed inside dry air fed single wire-duct reactor under positive... more
This paper is aimed to study the characteristics of the positive dc corona discharges in single wire-duct electrostatic precipitators. Therefore, the corona discharges were formed inside dry air fed single wire-duct reactor under positive dc voltage at the normal atmospheric conditions. The corona current-voltage characteristics curves have been measured in parallel with the ozone concentration generated inside the reactor under different discharge conditions. The corona current-voltage characteristics curves have agreed with a semi empirical equation derived from the previous studies. The experimental results of the ozone concentration generated inside the reactor were formulated in the form of an empirical equation included the different parameters that were studied experimentally. The obtained equations are valid to expect both the current-voltage characteristics curves and the corresponding ozone concentration that generates with the positive dc corona discharges inside single w...
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In this paper, Sliding Discharge (SD) was experimentally studied to generate homogeneous surface discharge on the outer surface of a poly(methyl methacrylate) (PMMA) tube. Cylindrical configuration was employed in order to eliminate edge... more
In this paper, Sliding Discharge (SD) was experimentally studied to generate homogeneous surface discharge on the outer surface of a poly(methyl methacrylate) (PMMA) tube. Cylindrical configuration was employed in order to eliminate edge effect and to generate uniform plasma along the electrodes. AC high voltage was applied to generate large and stable surface plasma with different geometric and the electric parameters. From the analysis of electrical measurements and photographs, it has been shown that the SD generation depends strongly on the magnitude of electric field between outer electrodes. The plasma sheet was very homogeneous along the tube axis with DC component, small SD gap, and high frequency.
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キーワード:静電気,電気集塵,再飛散,誘導荷電 * パナソニック環境エンジニアリング株式会社 (〒486-8524 愛知県春日井市鷹来町字上仲田3905番3) Panasonic Environmental Systems & Engineering Co., Ltd, 3905, Takaki-cho Kasugai-city, Aichi-pref., 486-8524, Japan ** パナソニックエコシステムズ株式会社 (〒486-8522... more
キーワード:静電気,電気集塵,再飛散,誘導荷電 * パナソニック環境エンジニアリング株式会社 (〒486-8524 愛知県春日井市鷹来町字上仲田3905番3) Panasonic Environmental Systems & Engineering Co., Ltd, 3905, Takaki-cho Kasugai-city, Aichi-pref., 486-8524, Japan ** パナソニックエコシステムズ株式会社 (〒486-8522 愛知県春日井市鷹来町字下仲田 4017番) Panasonic Ecology Systems Co., Ltd, 4017, Takaki-cho Kasugai-city, Aichi-pref., 486-8522, Japan *** 国立大学法人 豊橋技術科学大学 (〒441-8580 愛知県豊橋市天伯町雲雀ヶ丘 1-1) Toyohashi University of Technology, 1-1, Hibarigaoka, Tenpaku-ku, Toyohashi-city, Aich-pref., 441-8580, Japan 1 katatani.atsushi@jp.panasonic.com その結果,極板に粉塵が付着・堆積していることを確認 した.また,いったん極板に付着した粉塵が再飛散する 際,誘導荷電作用により帯電し,電界中のクーロン力で 捕集されることを確認した.即ち,コロナ放電を利用し なくても,粉塵が捕集されることを確認した.しかし, この先行研究では,集塵率を測定しなかったので,集塵 率自体が不明であった.また,極板の風上端部と風下端 部に,粉塵が多く付着する傾向があったが,この点に関 する検討が必要であると考えた. そこで,今回の研究では,先行研究と同様の構造の ESP を用い,室内大気塵に関する集塵率を測定すること とした.また,2種類の板厚の極板を用い,端部条件の 変化が集塵率に及ぼす影響を確認することにした.
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An electrostatic precipitator (ESP) has been widel y used for gas treatment such as flue gas cleaning and room air cleaning. Collect ion of particle matter (PM) in diesel exhaust is a new application of ESP. It shows very high collection... more
An electrostatic precipitator (ESP) has been widel y used for gas treatment such as flue gas cleaning and room air cleaning. Collect ion of particle matter (PM) in diesel exhaust is a new application of ESP. It shows very high collection efficiency for the PM in diesel exhaust, which includes fine particles ra nging from some nanometer to some micrometer in diameter. Low pressure drop is anothe r advantage of the ESP against conventional PM collection technologies like diesel particulate filter (DPF). However, PM slip due to abnormal reentrainment sometimes tak es place because electric conductivity of the PM is high enough to lose charg es on the collection plate. In this study, to cope with this problem, porous c eramic foam consisting of many fine holes is used to suppress the reentrainme nt. The ceramic foam placed on a collecting plate of an ESP is expected to enhance c apacity to keep the PM. It is also expected to reduce the velocity of the gas flow in the vicinity of the col...
大気圧低温プラズマは被照射物に対して熱負荷をかけずに,化学的に活性なラジカルによるプ ラズマプロセスを行うことが可能である.また対象物表面に非接触で照射でき,局所的なプラズ マプロセスが可能であることから,身体表面の疾患治療や虫歯治療などの医療分野への応用研究 が注目されている.プラズマジェットの照射により,1)DNA 分子が切断される 2)ウイルス 粒子はコートタンパクの損傷を受けさらに内包する遺伝子が損傷を受ける 3)大腸菌や枯草菌芽... more
大気圧低温プラズマは被照射物に対して熱負荷をかけずに,化学的に活性なラジカルによるプ ラズマプロセスを行うことが可能である.また対象物表面に非接触で照射でき,局所的なプラズ マプロセスが可能であることから,身体表面の疾患治療や虫歯治療などの医療分野への応用研究 が注目されている.プラズマジェットの照射により,1)DNA 分子が切断される 2)ウイルス 粒子はコートタンパクの損傷を受けさらに内包する遺伝子が損傷を受ける 3)大腸菌や枯草菌芽 胞はまず細胞膜が損傷を受けてラジカルの透過性が高まり,徐々に内部のタンパクや遺伝子など が損傷をうける[1]。プラズマ照射により真核生物である出芽酵母が受けるストレスの評価を、 DNA 修復に関わる遺伝子の発現に基づいて 行った. RNR2,HSP26の 2 種類のプロモー ター領域をレポーター遺伝子(lacZ)の上流 に結合し,出芽酵母株 DF5 に導入し,レポータ ー遺伝子の発現量に基づいて DNA 損傷の検 出を行った(図 1).薬品などによるストレス応 答の検出結果を図 2 に示す.アルキル化剤で あるMMSではDNA修復に関わる RNR2遺伝 子のプロモーターの活性が上昇しており, DNA が損傷を受けていることが確認できた. プラズマジェット曝露の結果を図 3 に示す. 変異原性の強い MMS と同様の効果が認めら れる. Ar に比べ He プラズマジェットは短 い照射時間で高い活性の増大(DNA損傷)が 確認された.
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Binding effect of the antitumor dirhodium (II) compounds to the plasmid pUC19 DNA has been studied under different molar ratio of Rh (II) compounds to base pair of pUC19 DNA (Rf) and time. The electrophoresis results indicate that Rh... more
Binding effect of the antitumor dirhodium (II) compounds to the plasmid pUC19 DNA has been studied under different molar ratio of Rh (II) compounds to base pair of pUC19 DNA (Rf) and time. The electrophoresis results indicate that Rh binding affect the DNA conformation and therefore, Rh-DNA migrated in the different position comparing with native DNA. At high value of Rf ICP-MS (Inductively Coupled Plasma Mass Spectrometry) measurement confirmed that 46% of Rh binds to DNA. In vitro result shows that DNA synthesis was inhibited by the Rh binding.
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A fast detection method for airborne bio-particles (BPs) using electrostatic precipitation (ESP) to enhance trapping and dielectric barrier discharge (DBD) to break the cell wall is demonstrated using Bacillus subtilis as model bacteria.... more
A fast detection method for airborne bio-particles (BPs) using electrostatic precipitation (ESP) to enhance trapping and dielectric barrier discharge (DBD) to break the cell wall is demonstrated using Bacillus subtilis as model bacteria. Experimental result shows that 50 ...
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Research Interests: Biology, DNA replication, Medicine, DNA, Humans, and 15 moreEscherichia coli, Polymerase Chain Reaction, Reaction Time, Nucleic Acids, Plasmids, Inductively Coupled Plasma Mass Spectrometry, In Vivo, Gel electrophoresis, Protein Binding, Antineoplastic Agents, Biochemistry and cell biology, Cell Growth, Dna Synthesis, restriction enzyme, and DNA Restriction Enzymes(Escherichia coli, Polymerase Chain Reaction, Reaction Time, Nucleic Acids, Plasmids, Inductively Coupled Plasma Mass Spectrometry, In Vivo, Gel electrophoresis, Protein Binding, Antineoplastic Agents, Biochemistry and cell biology, Cell Growth, Dna Synthesis, restriction enzyme, and DNA Restriction Enzymes)
(Escherichia coli, Polymerase Chain Reaction, Reaction Time, Nucleic Acids, Plasmids, Inductively Coupled Plasma Mass Spectrometry, In Vivo, Gel electrophoresis, Protein Binding, Antineoplastic Agents, Biochemistry and cell biology, Cell Growth, Dna Synthesis, restriction enzyme, and DNA Restriction Enzymes)
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Binding of Rhodium (II) acetate [Rh(2)(O(2)CCH(3))(4)] (Rh1) compound with plasmid pUC19 DNA has been studied using different molar ratio of Rh1. After incubation for 24hr at 37 degrees C, binding of the Rh1 to pUC19 DNA was confirmed by... more
Binding of Rhodium (II) acetate [Rh(2)(O(2)CCH(3))(4)] (Rh1) compound with plasmid pUC19 DNA has been studied using different molar ratio of Rh1. After incubation for 24hr at 37 degrees C, binding of the Rh1 to pUC19 DNA was confirmed by agarose gel electrophoresis. The electrophoretic results indicated the slower migration speed for the linearized pUC19 DNA. Conformation change of the DNA after Rh1 binding was also indicated at higher molar ratio of Rh1. The atomic force microscopy images showed that the Rh1 induced the conformation change to unwind pUC19 DNA. The Rh1-DNA complexes are observed very stable due to covalent bond. This study clearly demonstrates that [Rh(2)(O(2)CCH(3))(4)] reacts with pUC19 DNA and covalently binds to be stable Rh1-pUC19 DNA as interstrand adducts.
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6. Conclusion Atmospheric DBD has been applied to the wet state of Escherichia coli and bacteriophage-λ. Upon DBD treatment, both E. coli and λphage were immediately inactivated. The states of different biological components were... more
6. Conclusion Atmospheric DBD has been applied to the wet state of Escherichia coli and bacteriophage-λ. Upon DBD treatment, both E. coli and λphage were immediately inactivated. The states of different biological components were monitored during the ...
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Electrostatics is important in various system with different scale. In nano-scale, composition and function of the molecular system are affected by electrostatic force, therefore, electrostatics can be used for manipulation and analysis... more
Electrostatics is important in various system with different scale. In nano-scale, composition and function of the molecular system are affected by electrostatic force, therefore, electrostatics can be used for manipulation and analysis of molecules. For instance, DNA molecules can be manipulated for analysis using electrostatic force and optical tweezers. Combination of E-field and Laser provides more flexible tools for manipulation and for micro-pumping. At the meantime, in our earth, known as the global circuit, atmospheric ionic current (AIC) is flowing into the ground from ionosphere. This current could be affected by the sudden change of surface potential of earth prior to an intense earthquake. In medium scale, electrostatics is also important. Electric field generated by walking of human or animals is of interest. For instance, human is generating rather high voltage while walking, and the voltage waveform has specific spectrum profile. In this talk, several interesting phen...
Atmospheric pressure discharge plasma has been widely studied with the intention to apply for many fields, especially to environmental protection. Diesel exhaust cleaning using the non-thermal plasma is one of the most promising... more
Atmospheric pressure discharge plasma has been widely studied with the intention to apply for many fields, especially to environmental protection. Diesel exhaust cleaning using the non-thermal plasma is one of the most promising applications. We have studied diesel exhaust cleaning using the combination of catalyst and discharge plasma. We have developed a novel way of combining honeycomb catalyst and discharge plasma named “honeycomb discharge”, in which seeding discharge generated by DBD is expanded into capillaries of honeycomb catalyst due to DC high voltage application. This paper presents a new configuration of honeycomb discharge reactor which can be operated by a single high voltage power supply. NOx reduction performance using the newly proposed honeycomb discharge reactor and simulated diesel exhaust is presented in this paper. Characteristics of the capillary discharge such as stability, uniformity, onset / flashover voltage and power consumption, were observed against va...
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Exhaust gas from diesel cars has caused serious air pollution in urban areas. In this study, electrostatic precipitator (ESP) was investigated, with attention to suppress abnormal reentrainment for conductive diesel particles. A dust... more
Exhaust gas from diesel cars has caused serious air pollution in urban areas. In this study, electrostatic precipitator (ESP) was investigated, with attention to suppress abnormal reentrainment for conductive diesel particles. A dust pocket has been developed, consisting of a metal mesh backed with a barrier discharge electrode. It was installed downstream of the collecting electrode. Conductive particles touching to
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Mechanism of inactivation of bio-particles exposed to non-thermal plasma (NTP), namely, dielectric barrier discharge (DBD), and plasma jet (PJ), has been studied using E. coli, B. subtilis spore, S. cerevisiae and bacteriophages. States... more
Mechanism of inactivation of bio-particles exposed to non-thermal plasma (NTP), namely, dielectric barrier discharge (DBD), and plasma jet (PJ), has been studied using E. coli, B. subtilis spore, S. cerevisiae and bacteriophages. States of different biological components were monitored during the course of inactivation. Analysis of green fluorescent protein, GFP, introduced into E. coli. or B. subtiles spore cells proved that radicals generated by NTP penetrate into microbes, destroying the cell membrane and finally damage the genes. We have evaluated the damage of the bacteriophages. Bacteriophage λ having double stranded DNA was exposed to DBD, then DNA was purified and subjected to in vitro DNA packaging reactions. The re-packaged phages consist of the DNA from discharged phages and brand-new coat proteins were proved to be active, indicating that the damage of coat proteins is responsible for inactivation. M13 phages having single stranded DNA were also examined with the same ma...
Superhelices, which are induced by the twisting and coiling of double-helical DNA in chromosomes, are thought to affect transcription, replication, and other DNA metabolic processes. In this study, we report the effects of negative... more
Superhelices, which are induced by the twisting and coiling of double-helical DNA in chromosomes, are thought to affect transcription, replication, and other DNA metabolic processes. In this study, we report the effects of negative supercoiling on the unwinding activity of simian virus 40 large tumor antigen (SV40 TAg) at a single-molecular level. The supercoiling density of linear DNA templates was controlled using magnetic tweezers and monitored using a fluorescent microscope in a flow cell. SV40 TAg-mediated DNA unwinding under relaxed and negative supercoil states was analyzed by the direct observation of both single- and double-stranded regions of single DNA molecules. Increased negative superhelicity stimulated SV40 TAg-mediated DNA unwinding more strongly than a relaxed state; furthermore, negative superhelicity was associated with an increased probability of SV40 TAg-mediated DNA unwinding. These results suggest that negative superhelicity helps to regulate the initiation of...
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Conversion of heavy oils into light hydrocarbons has been experimentally studied using discharge plasma. The purpose of this study was to improve the understanding of the parameter characteristics for optimal design of the plasma reactors... more
Conversion of heavy oils into light hydrocarbons has been experimentally studied using discharge plasma. The purpose of this study was to improve the understanding of the parameter characteristics for optimal design of the plasma reactors on the conversion of heavy oils. The low molecular weight hydrocarbons obtained constitute NOx removal system using light olefin hydrocarbons, such as ethylene and propylene
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ABSTRACT Mechanism of inactivation of bio-particles exposed to dielectric barrier discharge, DBD, has been studied using E. coli and bacteriophages. States of different biological components were monitored during the course of... more
ABSTRACT Mechanism of inactivation of bio-particles exposed to dielectric barrier discharge, DBD, has been studied using E. coli and bacteriophages. States of different biological components were monitored during the course of inactivation. Analysis of green fluorescent protein, GFP, introduced into E.coli cells proved that Non-thermal Plasma, NTP causes a prominent protein damages without cutting peptide bonds. We have developed a biological assay which evaluates in vitro DNA damage of the bacteriophages. Bacteriophage λ having double stranded DNA was exposed to DBD, then DNA was purified and subjected to in vitro DNA packaging reactions. The re-packaged phages consist of the DNA from discharged phages and brand-new coat proteins. Survival curves of the re-packaged phages showed extremely large D value (D = 25 s) compared to the previous D value (D = 3 s) from the discharged phages. The results indicate that DNA damage hardly contributed to the inactivation, and the damage in coat proteins is responsible for inactivation of the phages. M13 phages having single stranded DNA were also examined with the same manner. In this case, damage to DNA was as severe as that of the coat proteins.
The inactivation mechanism of virus and bacteria by atmospheric discharge plasma has been studied actively. However, predominant factors in the inactivation are not clear at all. Because the atmospheric discharge plasma includes a lot of... more
The inactivation mechanism of virus and bacteria by atmospheric discharge plasma has been studied actively. However, predominant factors in the inactivation are not clear at all. Because the atmospheric discharge plasma includes a lot of possible inactivation factors such as active oxygen species, ozone and ultraviolet rays, it is difficult to analyze each effect separately. In this study, bacteriophage lambda
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Condition of sliding discharge has been investigated to ionize inside honeycomb catalysts for exhaust gas cleaning. In order to improve selectivity and energy efficiency to promote plasma chemical reactions, combination of plasma and... more
Condition of sliding discharge has been investigated to ionize inside honeycomb catalysts for exhaust gas cleaning. In order to improve selectivity and energy efficiency to promote plasma chemical reactions, combination of plasma and catalyst is effective. Packed bed is connected to capillary tubes. Ac discharge is formed in the packed bed, and with the dc electric field ionization can be
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We show herein that a single T4 phage DNA molecule which was transformed from coiled to globular state, was trapped directly by NdrYAG laser (1064 nm). We also estimated trapping force of globular DNA molecules.
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ABSTRACT This paper deals with optoelectrostatic pumping of conductive liquids in microchannels. In this technique, the fluid is heated by laser irradiation and exposed to an external ac electric field. If the fluid permittivity and... more
ABSTRACT This paper deals with optoelectrostatic pumping of conductive liquids in microchannels. In this technique, the fluid is heated by laser irradiation and exposed to an external ac electric field. If the fluid permittivity and conductivity vary with temperature, the electric body force is generated, and pumping effect can be achieved. A numerical model of this phenomenon is presented, and it is based on the finite-element analysis of the electric, thermal, and flow fields. The numerical predictions are compared with the experimental data, with a reasonable qualitative agreement.
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ABSTRACT We studied the oxidative coupling and reforming of CH4 with CO2 to C2H4, CO, and H2 using a pulsed plasma with a pulse frequency ranging from 166 to 3050 PPS. The largest selectivity of C2H4 was 64% with 31% CH4 conversion and... more
ABSTRACT We studied the oxidative coupling and reforming of CH4 with CO2 to C2H4, CO, and H2 using a pulsed plasma with a pulse frequency ranging from 166 to 3050 PPS. The largest selectivity of C2H4 was 64% with 31% CH4 conversion and 24% CO2 conversion at 2920 PPS and 500 °C. Selectivities of CO and H2 were about 20% and 100%, respectively. Ratios of H2 to CO and (CO + CO2) were, respectively, 7.1 and 2.5, which are acceptable for methanol production from (CO + H2) and (CO2 + H2) over a catalyst. The results indicated that the pulsed plasma with a high frequency can promote conversion of CH4 and CO2. The energy efficiency of the pulsed plasma was improved using a high pulse frequency and a high reaction temperature. We suggested that a pulsed plasma with a high pulse frequency is useful for oxidative coupling and reforming of CH4 with CO2 in industry.
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ABSTRACT Droplet-based micro total analysis systems have been investigated by many researchers and used in bioengineering. In these systems, individual water-in-oil (W/O) droplets perform as microreactors. To manipulate W/O droplets, an... more
ABSTRACT Droplet-based micro total analysis systems have been investigated by many researchers and used in bioengineering. In these systems, individual water-in-oil (W/O) droplets perform as microreactors. To manipulate W/O droplets, an electrostatic field is one of the most useful tools. For example, by applying a direct current (dc) electric field between electrodes soaked in oil, W/O droplets in the electrode spacings reciprocate due to dielectrophoretic force and Coulomb force. In this paper, gene transformation to Escherichia coli cells by using the droplet recycling is carried out. A droplet containing E. coli and plasmid deoxyribonucleic acid was located between the electrodes, and dc voltage was applied. As a result, the transformants were obtained. Therefore, the gene transformation by the electrostatic droplet manipulation was confirmed. In this method, sample volume can be smaller than that of conventional method. Therefore, our novel gene transfection method is expected to be a new tool in miniaturized bioengineering.
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Department of Chemical Engineering, National University of Tucuman, Argentine Republic Ave. Independencia 1800, (4000) San Miguel de Tucuman, Argentina Fax: +54-381-4363004 E-mail: gprieto@herrera.unt.edu.ar E-mail:... more
Department of Chemical Engineering, National University of Tucuman, Argentine Republic Ave. Independencia 1800, (4000) San Miguel de Tucuman, Argentina Fax: +54-381-4363004 E-mail: gprieto@herrera.unt.edu.ar E-mail: oprieto@herrera.unt.edu.ar E-mail: cgay@ ...