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The MspI restriction-modification system, which recognizes the sequence 5'-CCGG-3', has been previously cloned and sequenced (1). We subcloned the methyltransferase gene (M.MspI) downstream of the ptac promoter in the multicopy... more
Mucosal (and in minor extent transcutanous) stimulation can induce local or distant mucosa secretory IgA. Liposomes and other vesicles as mucosal and transcutaneous adjuvants are attractive alternatives to parenteral vaccination.... more
A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium... more
One limiting factor in studies of tuberculosis and leprosy is the difficulty of genetic analysis and manipulation of mycobacteria. Two approaches were adopted for the construction of vectors, based on different Escherichia coli plasmids... more
Gene electrotransfer is an established method for plasmid delivery into different tissues. Contrary to extensive in vitro studies demonstrating increased gene electrotransfer by changing the electric field direction during the pulse... more
One major barrier limiting the transfection efficiency of polyplexes is poor endosomal release, especially when small particles are applied. In an approach to overcome this barrier, covalent attachment of the membrane-active peptide... more
KATHLEEN M. HESS*, MATTHEW W. DUDLEY*, DAVID G. LYNN*t, ROLF D. JOERGERf, AND ANDREW N. BINNSt *Searle Chemistry Laboratory, 5735 Ellis Avenue, The University of Chicago, Chicago IL 60637; and tPlant Science Institute, Department of... more