Arzum Erdem
Ege University, Faculty of Pharmacy, Faculty Member
- Arzum Erdem received Bachelor in Pharmacy from Ege University, Izmir, Turkey in 1993. She received the master degree ... moreArzum Erdem received Bachelor in Pharmacy from Ege University, Izmir, Turkey in 1993. She received the master degree in 1996 and PhD degree in 2000 in the Department of Analytical Chemistry at the same university in Izmir. She worked as an Assistant Professor from 2000 to 2003 and as an Associated Professor from 2003 to 2009 at the Analytical Chemistry Department of the Faculty of Pharmacy in Ege University. She has been working at the same department as a Full Professor since 2009. Arzum Erdem was awarded by the Turkish Academy of Sciences (TÜBA) as the one of highly skilled young twenty Turkish scientists elected in 2001 under the TÜBA-GEBİP fellowship programme. She received "Juniour Science Award" in 2006, and "Science Award" in 2015 given by The Scientific and Technological Research Council of Turkey (TÜBİTAK). She was elected as the Associate member of TÜBA in 2007, and elected as the Principal member of TÜBA in 2016. Arzum Erdem was elected as the Special Committee member of Association of Academies and Societies of Sciences in Asia (AASSA)- Women In Science and Engineering (WISE) for the period of 2017-2019 and 2019-2021. She was elected as a fellow of Royal Society of Chemistry (FRSC) in December, 2017.Prof. Arzum Erdem has authored or co-authored more than 190 papers in refereed journals and conference proceedings, she has given more than 20 invited talks in international meetings and conferences, is the co-author of 15 book chapters and review papers. She has received 6,625 (without self-citations: 5316) citations according to the records on Web of Science (WoS) obtained in October 2021 with h-index: 43.(Arzum Erdem received Bachelor in Pharmacy from Ege University, Izmir, Turkey in 1993. She received the master degree in 1996 and PhD degree in 2000 in the Department of Analytical Chemistry at the same university in Izmir. She worked as an Assistant Professor from 2000 to 2003 and as an Associated Professor from 2003 to 2009 at the Analytical Chemistry Department of the Faculty of Pharmacy in Ege University. She has been working at the same department as a Full Professor since 2009. Arzum Erdem was awarded by the Turkish Academy of Sciences (TÜBA) as the one of highly skilled young twenty Turkish scientists elected in 2001 under the TÜBA-GEBİP fellowship programme. She received "Juniour Science Award" in 2006, and "Science Award" in 2015 given by The Scientific and Technological Research Council of Turkey (TÜBİTAK). She was elected as the Associate member of TÜBA in 2007, and elected as the Principal member of TÜBA in 2016. Arzum Erdem was elected as the Special Committee member of Association of Academies and Societies of Sciences in Asia (AASSA)- Women In Science and Engineering (WISE) for the period of 2017-2019 and 2019-2021. She was elected as a fellow of Royal Society of Chemistry (FRSC) in December, 2017.Prof. Arzum Erdem has authored or co-authored more than 190 papers in refereed journals and conference proceedings, she has given more than 20 invited talks in international meetings and conferences, is the co-author of 15 book chapters and review papers. She has received 6,625 (without self-citations: 5316) citations according to the records on Web of Science (WoS) obtained in October 2021 with h-index: 43.)edit
Research Interests: Chemistry, Analytical Chemistry, Electrochemistry, Carbon, Nanoparticle, and 12 moreNanotechnology, Medicine, DNA, Magnetic nanoparticles, Nucleic acid hybridization, Scanning Electron Microscope, Fourier transform infrared spectroscopy, Magnetic Nanoparticles, Electrodes, Magnetite Nanoparticles, Differential Pulse Voltammetry, and electrochemical biosensor
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Abstract In this study, lysozyme (LYS) detection was realized by impedimetric aptasensor that consist of aptamer immobilized carbon nanofibres modified screen printed electrodes (CNF-SPEs). Under this aim, NH2-functionalized LYS-specific... more
Abstract In this study, lysozyme (LYS) detection was realized by impedimetric aptasensor that consist of aptamer immobilized carbon nanofibres modified screen printed electrodes (CNF-SPEs). Under this aim, NH2-functionalized LYS-specific aptamer was immobilized to surface of CNF-SPEs via passive adsorption. The step-by-step fabrication process of the aptasensor and the detection of LYS was performed. During the aptasensor fabrication primarily electrochemical characterization was done via cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Aptamer-LYS complex formation caused prevention of electron transfer reaction on the interface and thus charge transfer resistance (Rct) increased. Based on this phenomenon, LYS could be quantitatively detected in accordance with the change at Rct values obtained by EIS in the presence of [Fe(CN)6]3–/4– while increasing LYS concentration. Under optimum conditions, the detection limit of the aptasensor was found to be 0.36 µg/mL. The proposed aptasensor was also used for the determination of LYS in fetal bovine serum samples with a detection limit of 1.89 µg/mL. Our assay in combination with the eight-channel array of electrodes was also used for LYS detection to present its implementation to nucleic acid-based array system.
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Abstract A class of well-defined hyperbranched polymers “Dendrimers” are nano-sized, radially symmetric molecules with well-defined and monodisperse structure having a symmetric core, an inner and an outer shell. They have biological... more
Abstract A class of well-defined hyperbranched polymers “Dendrimers” are nano-sized, radially symmetric molecules with well-defined and monodisperse structure having a symmetric core, an inner and an outer shell. They have biological properties such as self-assembling, well-defined globular structures, variable chemical composition, chemical stability, low cytotoxicity, and solubility. This chapter focuses on dendrimers with their application into the field of biosensors related to healthcare applications.
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Research Interests: Chemistry, DNA, Dna Sequencing, Biosensor, DNA hybridization, and 2 moreAptamer and Colloidal Gold(Aptamer and Colloidal Gold)
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The modification of graphene oxide (GO) onto the surfaces of chemically activated pencil graphite electrodes (PGEs) was performed herein, and then these electrodes were applied for the first time on voltammetric monitoring of miRNAs. The... more
The modification of graphene oxide (GO) onto the surfaces of chemically activated pencil graphite electrodes (PGEs) was performed herein, and then these electrodes were applied for the first time on voltammetric monitoring of miRNAs. The specific recognition of miRNA-34a, which has been related to Alzheimer disease, was explored in the presence of DNA-RNA hybridization by using CA/GO/PGEs in combination with differential pulse voltammetry (DPV) technique. The characterization of CA/GO/PGE was investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The effect of GO concentration, DNA probe concentration and miRNA-34a concentration upon to biosensor response was optimized, and accordingly, the selectivity of miRNA-34a biosensor was tested under the optimum conditions.
Research Interests: Materials Science, Biomedical Engineering, Graphene, Cyclic Voltammetry, Medicine, and 15 moremiRNA, Humans, Graphite, Dielectric Spectroscopy, Biosensor, Graphene Oxide, microRNAs, Electrodes, Electrode, Alzheimer Disease, Biosensing Techniques, Graphite oxide, DNA probes, Differential Pulse Voltammetry, and electrochemical biosensor
In our study, graphene oxide (GO) modified graphite electrodes were used for sensitive and selective impedimetric detection of miRNA. After chemical activation of pencil graphite electrode (PGE) surface using covalent agents (CA), GO... more
In our study, graphene oxide (GO) modified graphite electrodes were used for sensitive and selective impedimetric detection of miRNA. After chemical activation of pencil graphite electrode (PGE) surface using covalent agents (CA), GO modification was performed at the surface of chemically activated PGE. Then, CA‐GO‐PGEs were applied for impedimetric miRNA detection. The microscopic and electrochemical characterization of CA‐GO‐PGEs was performed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The optimization of experimental conditions; such as GO concentration, DNA probe concentration and miRNA target concentration was performed by using EIS technique. After the hybridization occurred between miRNA‐34a RNA target and its complementary DNA probe, the hybrid was immobilized onto the surface of CA‐GO‐PGEs. Then, the impedimetric detection of miRNA‐DNA hybridization was performed by EIS. The selectivity of our assay was also tested under the opti...
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Recently, the low-cost effective biosensing systems based on advanced nanomaterials have received a key attention for development of novel assays for rapid and sequence-specific nucleic acid detection. The electrochemical biosensor based... more
Recently, the low-cost effective biosensing systems based on advanced nanomaterials have received a key attention for development of novel assays for rapid and sequence-specific nucleic acid detection. The electrochemical biosensor based on reduced graphene oxide (rGO) modified disposable pencil graphite electrodes (PGEs) were developed herein for electrochemical monitoring of DNA, and also for monitoring of biointeraction occurred between anticancer drug, Daunorubicin (DNR), and DNA. First, rGO was synthesized chemically and characterized by using UV-Vis, TGA, FT-IR, Raman Spectroscopy and SEM techniques. Then, the quantity of rGO assembling onto the surface of PGE by passive adsorption was optimized. The electrochemical behavior of rGO-PGEs was examined by cyclic voltammetry (CV). rGO-PGEs were then utilized for electrochemical monitoring of surface-confined interaction between DNR and DNA using differential pulse voltammetry (DPV) technique. Additionally, voltammetric results wer...
Research Interests: Materials Science, Analytical Chemistry, Biomedical Engineering, Electrochemistry, Graphene, and 15 moreNanomaterials, Cyclic Voltammetry, Medicine, DNA, Graphite, Electrochemical Impedance Spectroscopy, Animals, Dielectric Spectroscopy, Biosensor, Fishes, Electrodes, Biosensing Techniques, Differential Pulse Voltammetry, Electrochemical Biosensors, and Daunorubicin
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Chitosan (CHIT) and carbon nanofiber (CNF) modified pencil graphite electrodes (PGEs) were developed for the first time in the present study for enhanced monitoring of DNA hybridization. CHIT‐CNF modified PGE, CHIT modified PGE and... more
Chitosan (CHIT) and carbon nanofiber (CNF) modified pencil graphite electrodes (PGEs) were developed for the first time in the present study for enhanced monitoring of DNA hybridization. CHIT‐CNF modified PGE, CHIT modified PGE and unmodified PGE were firstly characterized by scanning electrone microscopy (SEM), and the electrochemical behaviour of each electrode was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The optimization of experimental studies; CNF concentration, or DNA probe concentration was performed by differential pulse voltammetry (DPV) technique. The sequence‐selective DNA hybridization related to Hepatitis B virus (HBV) was then performed in the case of hybridization between HBV probe and its target DNA, or different non‐complementary DNA sequences (NC1 and NC2), and also the hybridization case in the mixture samples containing both target and NC sequences in the ratio of 1 : 1. CHIT‐CNF‐PGEs presented very effective discr...
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Aptamers are defined as new generation of nucleic acids which has recently presented the promising spesifications over to antibodies. They can be produced in vitro by Systematic Evolution of Ligands by EXponential Enrichment (SELEX), and... more
Aptamers are defined as new generation of nucleic acids which has recently presented the promising spesifications over to antibodies. They can be produced in vitro by Systematic Evolution of Ligands by EXponential Enrichment (SELEX), and have the ability to recognize selectively and sensitively their targets; protein, toxin, drug or cell targets. Thus, they have a wide range of applications in different areas, such as, drug delivery, imaging and biosensing. Accordingly, an increasing number of studies related to aptamer based sensors “aptasensors” have been introduced in the literature. The recent studies on development of aptasensor technologies, which were applied for toxin detection, have been overviewed herein.
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Research Interests: Chemistry, Analytical Chemistry, Electrochemistry, Nanotechnology, Cyclic Voltammetry, and 11 moremiRNA, Electroanalytical Chemistry, Nanofiber, Electrochemical Impedance Spectroscopy, Dielectric Spectroscopy, Biosensor, Carbon Nanofiber, Screen Printed Electrode, Electrode, Carbon Nanofibers, and Differential Pulse Voltammetry
The impedimetric aptasensor for Thrombin (THR) was developed for the first time herein by measuring changes at the charge‐transfer resistance, Rct upon to proteinaptamer complex formation. After covalent activation of pencil graphite... more
The impedimetric aptasensor for Thrombin (THR) was developed for the first time herein by measuring changes at the charge‐transfer resistance, Rct upon to proteinaptamer complex formation. After covalent activation of pencil graphite electrode (PGE) surface using covalent agents, amino linked aptamer (APT) was immobilized onto activated PGE surface. Then APT‐THR interaction was explored by electrochemical impedance spectroscopy (EIS). After the optimization of experimental conditions (e.g., APT and THR concentration, immobilization and interaction times), the selectivity of impedimetric aptasensor was tested in the presence of other biomolecules: factor Va and bovine serum albumine (BSA) both in buffer media, or in diluted fetal bovine serum (FBS).
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Abstract Laboratory analyzers are an invaluable resource but they have several limitations; for instance, they are more suited for a hospital setting and used by trained personnel who can operate the machine and interpret the results... more
Abstract Laboratory analyzers are an invaluable resource but they have several limitations; for instance, they are more suited for a hospital setting and used by trained personnel who can operate the machine and interpret the results accurately. However, biosensors have many advantages, such as high sensitivity, rapid response, and user-friendly. It is essential to meet the necessities of IVD technologies in the market to establish biosensors developed for diagnosis of diseases. The demand for the accurate monitoring of biomarkers related to the most important fatal diseases is further driving forces for the development of novel analytical systems based on diagnostics. Thanks to the recent developments toward commercial biosensors, early diagnosis of diseases would significantly improve prognosis and survival rates by reducing disease burden, helping social development, and increasing life quality. This chapter is mainly focused on an overview of recent commercial biosensors developed for diagnosis of cancers, cardiac diseases, and other diseases.
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Optical biosensors have many advantages over traditional analytical methods. They enable the identification of several biological and chemical compounds directly, instantly, and without the need of labels. Their benefits include excellent... more
Optical biosensors have many advantages over traditional analytical methods. They enable the identification of several biological and chemical compounds directly, instantly, and without the need of labels. Their benefits include excellent specificity, sensitivity, compact size, and low cost. In this review, the main focus is placed on the nucleic acid-based optical biosensor technologies, including colorimetric, fluorescence, surface plasmon resonance (SPR), Evanescent-Wave Optical, Fiber optic and bioluminescent optical fibre. The fundamentals of each type of biosensor are briefly explained, and particular emphasis has been placed on the achievements which have been gained in the last decade on the field of diagnosis of infectious viral diseases. Concluding remarks concerning the perspectives of further developments are discussed.
Zip nucleic acid (ZNA)-based genomagnetic assay was developed herein for the electrochemical detection of microRNA-34a (miR-34a), which is related to neurological disorders and cancer. The hybridization between the ZNA probe and miR-34a... more
Zip nucleic acid (ZNA)-based genomagnetic assay was developed herein for the electrochemical detection of microRNA-34a (miR-34a), which is related to neurological disorders and cancer. The hybridization between the ZNA probe and miR-34a target was performed in the solution phase; then, the resultant hybrids were immobilized onto the surface of magnetic beads (MBs). After magnetic separation, the hybrids were separated from the surface of MBs and then immobilized on the surface of pencil graphite electrodes (PGEs). In the case of a full-match hybridization, the guanine oxidation signal was measured via the differential pulse voltammetry (DPV) technique. All the experimental parameters that influenced the hybridization efficiency (i.e., hybridization strategy, probe concentration, hybridization temperature, etc.) were optimized. The cross-selectivity of the genomagnetic assay was tested against two different miRNAs, miR-155 and miR-181b, individually as well as in mixture samples. To ...
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After the COVID-19 pandemic started all over the world, great importance was placed on the development of sensitive and selective bioanalytical assays for the rapid detection of the highly pathogenic SARS-CoV-2 virus causing COVID-19... more
After the COVID-19 pandemic started all over the world, great importance was placed on the development of sensitive and selective bioanalytical assays for the rapid detection of the highly pathogenic SARS-CoV-2 virus causing COVID-19 disease. In this present work, an impedimetric immunosensor was developed and applied for rapid, reliable, sensitive and selective detection of the SARS-CoV-2 S1 protein. To detect the SARS-CoV-2 virus, targeting of the spike S1 protein was achieved herein by using S1 protein-specific capture antibody (Cab-S1) immobilized screen-printed electrode (SPE) in combination with the electrochemical impedance spectroscopy (EIS) technique. With the impedimetric immunosensor, the detection limit for S1 protein in buffer medium was found to be 0.23 ng/mL (equal to 23.92 amol in 8 µL sample) in the linear concentration range of S1 protein from 0.5 to 10 ng/mL. In the artificial saliva medium, it was found to be 0.09 ng/mL (equals to 9.36 amol in 8 µL sample) in the...
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A handheld USB-powered instrument developed for the electrochemical detection of nucleic acids and biomolecular interactions is presented. The proposed instrument is capable of scanning ± 2.25 V while measuring currents up to ±10 mA, with... more
A handheld USB-powered instrument developed for the electrochemical detection of nucleic acids and biomolecular interactions is presented. The proposed instrument is capable of scanning ± 2.25 V while measuring currents up to ±10 mA, with a minimum current resolution of 6.87 pA. Therefore, it is suitable for nucleic acid sensors, which have high background currents. A low-cost microcontroller with an on-chip 16-bit analog-to-digital converter, 12-bit digital-to-analog converter, and a built-in USB controller were used to miniaturize the system. The offset voltages and gain errors of the analog peripherals were calibrated to obtain a superior performance. Thus, a similar performance to those of the market-leader potentiostats was achieved, but at a fraction of their cost and size. The performance of the application of this proposed architecture was tested successfully and was found to be similar to a leading commercial device through a clinical application in the aspects of the detec...
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Electrochemical impedance spectroscopy (EIS) is applied for the detection of bacteria using bacteriophages as a bioprobe together with gold nanorods (GNRs).
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Paper-based biosensors are considered simple and cost-efficient sensing platforms for analytical tests and diagnostics. Here, a paper-based electrochemical biosensor was developed for the rapid and sensitive detection of microRNAs... more
Paper-based biosensors are considered simple and cost-efficient sensing platforms for analytical tests and diagnostics. Here, a paper-based electrochemical biosensor was developed for the rapid and sensitive detection of microRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. Hydrophobic barriers to creating electrode areas were manufactured by wax printing, whereas a three-electrode system was fabricated by a simple stencil approach. A carbon-based working electrode was modified using either reduced graphene oxide or molybdenum disulfide nanosheets modified with gold nanoparticle (AuNPs/RGO, AuNPs/MoS2) hybrid structures. The resulting paper-based biosensors offered sensitive detection of miRNA-155 and miRNA-21 by differential pulse voltammetry (DPV) in only 5.0 µL sample. The duration in our assay from the point of electrode modification to the final detection of miRNA was completed within only 35 min. The detection limits for miRNA-21 and miRNA-155 were foun...
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Pyrrolizidine alkaloids (PAs) are produced by plants as secondary compounds that are the most widely distributed natural toxins. There have been many cases of human toxicity caused by consumption of toxic plant species, as herbal teas and... more
Pyrrolizidine alkaloids (PAs) are produced by plants as secondary compounds that are the most widely distributed natural toxins. There have been many cases of human toxicity caused by consumption of toxic plant species, as herbal teas and grain or grain products contaminated with PA-containing seeds have been reported. Companies that produce dried spices and tea leaves should examine the PA level in their products. For the first time in the literature, a simple and inexpensive electrochemical assay based on a single-use sensor was introduced for quantitative determination of senecionine (SEN) in the most frequently contaminated food sources. SEN was immobilized on a pencil graphite electrode surface by the passive adsorption technique. Differential pulse voltammetry (DPV) was used to evaluate the oxidation signal of SEN, which was observed to be around +0.95 V. The oxidation signal was specific to the SEN in the sample, and the current value was proportional to its concentration. Th...
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In the present work, chemically synthesized graphene nanosheets were used as electrode materials and their electrochemical properties were systematically characterized. The surface morphologies of graphene nanosheets were evaluated using... more
In the present work, chemically synthesized graphene nanosheets were used as electrode materials and their electrochemical properties were systematically characterized. The surface morphologies of graphene nanosheets were evaluated using Raman spectroscopy and transmission ...
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Electrochemical biosensor for the detection of DNA hybridization using the reduction signal of alpha-naphthol is described. A pencil graphite electrode was used as a working electrode. Capture probes were covalently attached on to the... more
Electrochemical biosensor for the detection of DNA hybridization using the reduction signal of alpha-naphthol is described. A pencil graphite electrode was used as a working electrode. Capture probes were covalently attached on to the pencil graphite electrode surface (PGE) at the 5' end amino group by using N-(dimethylamino)propyl-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (NHS) as a coupling agent on to PGE. After capture probe immobilization on to PGE surface; probe was hybridized with complementary biotinylated oligonucleotide. Alkaline phosphatase labeled with extravidin (Ex-AP) binds to biotinylated hybrid via biotin-avidin interaction. alpha-Naphthyl phosphate (alpha-NAP) was added and the reaction between alkaline phosphatase (AP) and alpha-NAP was occurred consequently as a substrate of AP, alpha-NAP reduction signal was obtained from this reaction, at -0.100 V by using differential pulse voltammetry (DPV). Other experimental parameters were studied such as; optimizations of hybridization time, and the concentrations of capture probe, biotinylated oligonucleotide and enzyme.
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Graphene-oxide and ionic liquid composite-modified pencil graphite electrodes (GO-IL-PGEs) were developed and used as a sensing platform for breast cancer 1 (BRCA1) gene detection. The characterization of GO-IL modified electrodes was... more
Graphene-oxide and ionic liquid composite-modified pencil graphite electrodes (GO-IL-PGEs) were developed and used as a sensing platform for breast cancer 1 (BRCA1) gene detection. The characterization of GO-IL modified electrodes was executed by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The nucleic-acid hybridization was monitored by a differential pulse voltammetry (DPV) technique by directly measuring the guanine oxidation signal without using any indicator. The effects of the IL concentration, the probe concentration, and the hybridization time were optimized to the biosensor response. The limit of detection (LOD) was calculated in the concentration range of 2–10 μg/mL for the BRCA1 gene and found to be 1.48 µg/mL. The sensitivity of the sensor was calculated as 1.49 µA.mL/µg.cm2. The developed biosensor can effectively discriminate the complementary target sequence in comparison to a three-base-mismatched sequ...
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Proteinler organizmaların yapıtaşlarından birisi olup, hücre içerisindeki tüm mekanizmalarda görev alır. Karmaşık biyolojik sistemlerin araştırılmasında protein biyosensörleri önem taşımaktadır. Yüzey plazmon rezonansı, kuvartz kristal... more
Proteinler organizmaların yapıtaşlarından birisi olup, hücre içerisindeki tüm mekanizmalarda görev alır. Karmaşık biyolojik sistemlerin araştırılmasında protein biyosensörleri önem taşımaktadır. Yüzey plazmon rezonansı, kuvartz kristal mikroterazisi, kemilüminesans, elektroforez, floresans teknikleri ve elektrokimyasal yöntemler protein biyosensörlerinde kullanılan tekniklerden bazılarıdır. Son yıllarda protein araştırmalarında (protein-protein etkileşimleri ve protein kaynaklı hastalıkların incelenmesi gibi çalışmalar) elektrokimyasal teknikler büyük ilgi görmektedir. Bu derlemede, özellikle elektrokimyasal protein sensörlerindeki yeni yaklaşımlara değinilmiştir.
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Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive... more
Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV) were applied to examine the microscopic and electrochemical characterization of HaP and IL-modified biosensors. The interaction of curcumin with nucleic acids and polymerase chain reaction (PCR) samples was investigated by measuring the changes at the oxidation signals of both curcumin and guanine by differential pulse voltammetry (DPV) technique. The optimization of curcumin concentration, DNA concentration, and the interaction time was performed. The interaction of curcumin with PCR samples was also investigated by gel electrophoresis.