The genetic diversity of physical capacity in the Lithuanian population has a pattern similar to that of other populations. The knowledge of the individual genomes of the athletes is especially important for sports theory, practice and...
moreThe genetic diversity of physical capacity in the Lithuanian population has a pattern similar to that of other populations. The knowledge of the individual genomes of the athletes is especially important for sports theory, practice and medicine. This research is devoted to the issue of the effect of genetic factors on the components of sporting physical capacity. We have accumulated a sample of the Lithuanian elite athletes which was studied genetically according to a phenotype. We have created a DNA biobase of the Lithuanian elite athletes of various sporting disciplines and collected information about the genotypes and phenotypes of physical development and functional capacity of the athletes. This is the first time in Lithuania that the elite athletes were investigated according to allelic distribution of 6 candidate gene variants most associated with physical capacity. The genetic diversity of the physical capacity in the Lithuanian population has a pattern manifested by variati...
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Abstracts from the 52nd European Society of Human Genetics (ESHG) Conference, Gothenburg, Sweden, June 15-18, 2019: Posters : P18.14BMokytojų rengimo institutasVilniaus universitetasVytauto Didžiojo universiteta
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Įvadas. Daugelio įvairių mokslinių tyrimų pagrindu angiotenziną konvertuojančio fermento (ACE) ir α-aktinino-3 (ACTN3) genai vertinami kaip stiprūs genai kandidatai, lemiantys žmogaus fi zinį pajėgumą. Tačiau ACE I/D ir ACTN3 R/X...
moreĮvadas. Daugelio įvairių mokslinių tyrimų pagrindu angiotenziną konvertuojančio fermento (ACE) ir α-aktinino-3 (ACTN3) genai vertinami kaip stiprūs genai kandidatai, lemiantys žmogaus fi zinį pajėgumą. Tačiau ACE I/D ir ACTN3 R/X polimorfi zmų sąsajos su fi zinio pajėgumo fenotipu tyrimų, atliktų įvairiose populiacijose, duomenys buvo prieštaringi. Šio tyrimo tikslas – įvertinti ACE ir ACTN3 genetinių variantų reikšmę Lietuvos sportininkų raumenų jėgos fenotipui. Tirtųjų kontingentas ir tyrimo metodai. Ištirti 193 Lietuvos didelio meistriškumo sportininkai ir 250 bendrosios populiacijos asmenų (kontrolė). Genotipavimas buvo atliktas polimerazės grandininės reakcijos ir (arba) restrikcijos fragmentų ilgio polimorfi zmų analizės metodu. Išmatuoti sportininkų antropometriniai duomenys ir raumenų jėga (plaštakų suspaudimo jėga ir vertikalus šuolis). Rezultatai. Nustatyta, kad ACE I/I ir I/D genotipų dažniai tarp sportininkų didesni nei bendrojoje Lietuvos populiacijoje. ACTN3 X/X ir ACE...
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Different groups of muscles are functioning during exercising in different track and field athletics' events. That is why the sports massage of athletes has some peculiarities. Since the physiological state of athletes before and...
moreDifferent groups of muscles are functioning during exercising in different track and field athletics' events. That is why the sports massage of athletes has some peculiarities. Since the physiological state of athletes before and after the sports massage was not studied well enough, the purpose of this study is to examine the specificity of sports massage on particular track and field athletics' event and to evaluate physiological state of athletes by testing them after the massage. Lithuanian Olympic team candidates and forward training elite athletes were investigated (n=39). The skin of athletes heats up, also gains colour during the massage and it returns to normal state in approximately 15,8±3,1 minutes. The temperature, as an essential hbmeostasis' parameter, remains unchanged after the massage. The sports massage also has a significant influence on the painfulness, tumescence and indurations of muscles. These conditions decreased for 71,8 percent of athletes, disa...
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Mokytojų rengimo institutasVilniaus universitetasVytauto Didžiojo universiteta
GENETIC BIOMARKERS FOR MUSCULOSKELETAL INJURIES IN LITHUANIAN ATHLETES
The role of genetics, as an intrinsic factor, in research of sports performance increases with every passing year. The polymorphism rs970547 of the COL12A1 gene is one of the most promising genetic markers linked to soft-tissue injuries....
moreThe role of genetics, as an intrinsic factor, in research of sports performance increases with every passing year. The polymorphism rs970547 of the COL12A1 gene is one of the most promising genetic markers linked to soft-tissue injuries. This study aimed to investigate whether COL12A1 rs970547 genotypes are associated with elite Lithuanian athletes from high-risk various sports, such as running, throwing, jumping, and football. The study involved 293 Lithuanian elite athletes and 287 healthy untrained individuals from the Lithuanian population. The results of this study suggest that the rs970547 T allele and TT genotype were significantly over-represented in the total athlete group compared to controls (p < 0.05). There was a significantly lower C allele frequency in the sprint/power group (16.9%) as well as in footballers (19.4%) compared to controls (33.3%, p < 0.05). Positive selection analysis results showed that the derived allele experiences selection pressure within the...
The purpose of this study was to evaluate the effectiveness of a combined exercise program focused on the improvement of young women’s physical condition. Materials and methods. The study involved 62 healthy women (an average age of 29.4...
moreThe purpose of this study was to evaluate the effectiveness of a combined exercise program focused on the improvement of young women’s physical condition. Materials and methods. The study involved 62 healthy women (an average age of 29.4 years). More than 50 parameters were assessed (anthropometric indicators, physical capacity and motor abilities, functional status of the cardiorespiratory system, etc.). The structure of physical condition of the women was identified using the factor analysis. The transforming experiment lasted for 14 months. Results. The physical development indicators had the largest contribution (33.4%) to the total variance of physical characteristics of the subjects. The second factor related to the functional state and physical performance accounted for 21.6%, the third factor related to coordination abilities accounted for 19.8%, and the fourth factor related to physical fitness accounted for 10.3% of the total variance. Those four factors explained 85.1% of...
We aim to link fitness-related genotypes to the development of specific physical fitness phenotypes and a predisposition towards a specific sport category in Lithuanian elite athletes. Materials and methods. The study involved 193...
moreWe aim to link fitness-related genotypes to the development of specific physical fitness phenotypes and a predisposition towards a specific sport category in Lithuanian elite athletes. Materials and methods. The study involved 193 athletes (152 male and 41 female) and 250 controls. The athletes were stratified into four groups: endurance, mixed sports, speed / power, and team sports. Genotypes of the athletes were identified according to the genetic polymorphisms: ACE (rs1799752), ACTN3 (rs1815739), PPARGC1A (rs8192678) and PPARA (rs4253778). One-way analysis of variance and logistic regression modelling were used for testing the genotype–phenotype association. Results. The frequency of ACE I allele was higher in athletes than in controls, although only male athletes showed a significant difference from male controls. The PPARA C allele was more common in the athlete group than in the general population of Lithuania. There were no significant ACTN3 and PPARGC1A SNP allele / genotype...
Darbo tikslas – ištirti Lietuvos didelio meistriškumo sportininkų ACE [I/D] ir PGC1A [G/A] polimorfizmų reikšmę fiziniam pajėgumui. Fizinis pajėgumas yra tipiškas kiekybinis daugiaveiksnis paveldėjimas, kurio fenotipams poveikį daro tiek...
moreDarbo tikslas – ištirti Lietuvos didelio meistriškumo sportininkų ACE [I/D] ir PGC1A [G/A] polimorfizmų reikšmę fiziniam pajėgumui. Fizinis pajėgumas yra tipiškas kiekybinis daugiaveiksnis paveldėjimas, kurio fenotipams poveikį daro tiek daugelis genų, tiek aplinkos veiksniai. Dažniausiai sportuojančio žmogaus morfologiniai ir funkciniai rodikliai siejami su tam tikrų genų vieno nukleotido polimorfizmu. Skirtingi to paties geno aleliai gali turėti skirtingą įtaką žmogaus fiziniam išsivystymui ir darbingumui. ACE (angiotenziną konvertuojančio fermento) genas yra vienas iš genų, kurių įtaka žmogaus fiziniam pajėgumui buvo atskleista pirmiausia. Ankstesniais tyrimais nustatyta, kad šio geno Alu sekos intarpo buvimas ar nebuvimas ([I/D] polimorfizmas) gali būti susijęs su profesionaliu sportiniu pajė- gumu. Tačiau ACE [I/D] polimorfizmo tyrimų, atliktų įvairiose populiacijose, rezultatai yra prieštaringi. Mūsų tyrimo vienas iš uždavinių buvo nustatyti ir įvertinti ACE [I/D] polimorfizmo...
Energy homeostasis and different muscle contraction properties demands for interactive processes of diverse specialty enzymes, including important reactions of enzymes-regulators – adenosine monophosphate deaminase 1 (encoded by AMPD1...
moreEnergy homeostasis and different muscle contraction properties demands for interactive processes of diverse specialty enzymes, including important reactions of enzymes-regulators – adenosine monophosphate deaminase 1 (encoded by AMPD1 gene) and muscle-specific creatine kinase (encoded by CKM gene), in the human body during the physical activity. This research aims to analyse the effect of CKM (c.*800A >G, rs8111989) and AMPD1 (c.133C >T, p.Gln45Ter, rs17602729) gene polymorphisms on physical capacities of Lithuanian elite athletes. The study involved 150 Lithuanian elite athletes (60 females and 90 males, Olympic candidates, World’ and European Olympic medallists) and 150 controls (60 females and 90 males, healthy unrelated citizens of Lithuania without any competitive sport experience). The athletes were prospectively stratified into three groups according to the event duration and distance, spanning a spectrum from the endurance-oriented to the poweroriented athletes: ‘endur...
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
The main purpose of this study was to evaluate the association of phenotypic and genetic variety of Lithuanian elite male sprint and strength/poweroriented athletes according to the DNA markers (15 polymorphisms in PPARGC1B, PPARGC1A,...
moreThe main purpose of this study was to evaluate the association of phenotypic and genetic variety of Lithuanian elite male sprint and strength/poweroriented athletes according to the DNA markers (15 polymorphisms in PPARGC1B, PPARGC1A, PPARD, PPARG, PPARA, TFAM, ACE, ACTN3, AMPD1, MB, HIF1A, AGT, AGTR1, GALNT13 and CREM genes) associated with athletic performance. In the study decision tree, learning rules was created to associate athletes anthropometric and physical performance phenotypes with genotypes (according to 15 polymorphisms) in different sports (weightlifters, track and field, canoe-sprint). According the analysis, the 8 polymorphisms (out of 15) we have used constitute essential fraction of the genetic factors that influence physical performance phenotype of Lithuanian strength/power athletes. The results of this study would be valuable for the creation and implementation of personalized training programs for athletesVilniaus universiteta
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Vilniaus universitetasVytauto Didžiojo universitetasŠvietimo akademij
Sports genomics is the scientific discipline that focuses on the organization and function of the genome in elite athletes, and aims to develop molecular methods for talent identification, personalized exercise training, nutritional need...
moreSports genomics is the scientific discipline that focuses on the organization and function of the genome in elite athletes, and aims to develop molecular methods for talent identification, personalized exercise training, nutritional need and prevention of exercise-related diseases. It postulates that both genetic and environmental factors play a key role in athletic performance and related phenotypes. This update on the panel of genetic markers (DNA polymorphisms) associated with athlete status and soft-tissue injuries covers advances in research reported in recent years, including one whole genome sequencing (WGS) and four genome-wide association (GWAS) studies, as well as findings from collaborative projects and meta-analyses. At end of 2020, the total number of DNA polymorphisms associated with athlete status was 220, of which 97 markers have been found significant in at least two studies (35 endurance-related, 24 power-related, and 38 strength-related). Furthermore, 29 genetic markers have been linked to soft-tissue injuries in at least two studies. The most promising genetic markers include HFE rs1799945, MYBPC3 rs1052373, NFIA-AS2 rs1572312, PPARA rs4253778, and PPARGC1A rs8192678 for endurance; ACTN3 rs1815739, AMPD1 rs17602729, CPNE5 rs3213537, CKM rs8111989, and NOS3 rs2070744 for power; LRPPRC rs10186876, MMS22L rs9320823, PHACTR1 rs6905419, and PPARG rs1801282 for strength; and COL1A1 rs1800012, COL5A1 rs12722, COL12A1 rs970547, MMP1 rs1799750, MMP3 rs679620, and TIMP2 rs4789932 for soft-tissue injuries. It should be appreciated, however, that hundreds and even thousands of DNA polymorphisms are needed for the prediction of athletic performance and injury risk.
No cumulative genotype effect on 200-m sprint performance. (PNG 6 kb)
The MSTN gene is a negative regulator of muscle growth that is attracting attention as a candidate gene for physical performance traits. We hypothesised that variants of MSTN might be associated with the status of elite athlete. We...
moreThe MSTN gene is a negative regulator of muscle growth that is attracting attention as a candidate gene for physical performance traits. We hypothesised that variants of MSTN might be associated with the status of elite athlete. We therefore sought to study the potential role of MSTN in the physical performance of athletes by analysing the whole coding sequence of the MSTN gene in a cohort of Lithuanian elite athletes (n = 103) and non-athletes (n = 127). Consequently, two genetic variants were identified: the deletion of one of three adenines in the first intron (c.373+90delA, rs11333758) and a non-synonymous variant in the second exon (c.458A>G, p.Lys(K)153Arg(R), rs1805086). Among all samples, the MSTN rs1805086 Lys(K) allele was the most common form in both groups. Homozygous genotype for the less common Arg(R) allele was identified in only one elite canoe rower, and we could find no direct association between rs1805086 and successful results in elite athletes. Surprisingly, ...
51st Conference of the European-Society-of-Human-Genetics (ESHG) in conjunction with the European Meeting on Psychosocial Aspects of Genetics (EMPAG), Milan, ITALY,JUN 16-19, 2018. Posters: E-P18.03
Mokslinės praktinės konferencijos „Ateities sporto mokslas: moksliniais įrodymais pagrįsta sporto praktika ir medicina“ tezės, Vilnius, 2020 m. spalio mėn
Multiple genetic variants are known to influence athletic performance. These include polymorphisms of the muscle-specific creatine kinase (CKM) gene, which have been associated with endurance and/or power phenotypes. However, independent...
moreMultiple genetic variants are known to influence athletic performance. These include polymorphisms of the muscle-specific creatine kinase (CKM) gene, which have been associated with endurance and/or power phenotypes. However, independent replication is required to support those findings. The aim of the present study was to determine whether the CKM (rs8111989, c.*800A>G) polymorphism is associated with power athlete status in professional Russian and Lithuanian competitors. Genomic DNA was collected from 693 national and international standard athletes from Russia (n = 458) and Lithuania (n = 235), and 500 healthy non-athlete subjects from Russia (n = 291) and Lithuania (n = 209). Genotyping for the CKM rs8111989 (A/G) polymorphism was performed using PCR or micro-array analysis. Genotype and allele frequencies were compared between all athletes and non-athletes, and between non-athletes and athletes, segregated according to population and sporting discipline (from anaerobic-type...
Background and Objective. Based on the results of many studies, the angiotensinconverting enzyme (ACE) and the α-actinin-3 (ACTN3) genes are considered strong candidate genes associated with human physical performance. On the other hand,...
moreBackground and Objective. Based on the results of many studies, the angiotensinconverting enzyme (ACE) and the α-actinin-3 (ACTN3) genes are considered strong candidate genes associated with human physical performance. On the other hand, the data regarding the association of the ACE I/D and ACTN3 R/X polymorphisms with human physical performance in different populations have been conflicting. The objective of our research was to evaluate the significance of these genetic variants on muscle performance phenotype in Lithuanian athletes. Material and Methods. The study involved 193 Lithuanian elite athletes and 250 controls from the general Lithuanian population. Genotyping was performed by polymerase chain reaction and/ or restriction fragment length polymorphism analysis. Anthropometric measurements and muscle strength (grip strength and vertical jump) were measured. Results. It was determined that ACE I/I and I/D genotypes were more frequent in the athlete group compared with the ge...
The aim of this study was to investigate the association between the MCT1 (monocarboxylate transporter 1) A1470T polymorphism and positional roles in a large cohort of professional football players from five different countries. We...
moreThe aim of this study was to investigate the association between the MCT1 (monocarboxylate transporter 1) A1470T polymorphism and positional roles in a large cohort of professional football players from five different countries. We compared genotype distributions of the MCT1 A1470T polymorphism between football players (n=694) and non-athlete controls (n=781) from Italy, Poland, Lithuania, Ukraine and Malta, and we analyzed the MCT1 genotype distributions with respect to the players’ positions in the field (e. g. forwards, midfielders, defenders and goalkeepers). Genomic DNA was extracted from either buccal epithelium or peripheral blood using a standard protocol. In the pooled cohort of Italian, Polish, Lithuanian and Ukrainian football players, forwards (n=148) were more likely than controls (n=781) to possess the A allele (χ2=7.067, p=0.029, FDR q value 0.116), with a greater likelihood of having the AA genotype compared with the TT genotype (OR=1.97; C.I.=1.07-3.64; p=0.021, FDR...
To date, studies investigating the association between ACTN3 R577X and ACE I/D gene variants and elite sprint/power performance have been limited by small cohorts from mixed sport disciplines, without quantitative measures of performance....
moreTo date, studies investigating the association between ACTN3 R577X and ACE I/D gene variants and elite sprint/power performance have been limited by small cohorts from mixed sport disciplines, without quantitative measures of performance. To examine the association between these variants and sprint time in elite athletes. We collected a total of 555 best personal 100-, 200-, and 400-m times of 346 elite sprinters in a large cohort of elite Caucasian or African origin sprinters from 10 different countries. Sprinters were genotyped for ACTN3 R577X and ACE ID variants. On average, male Caucasian sprinters with the ACTN3 577RR or the ACE DD genotype had faster best 200-m sprint time than their 577XX (21.19 ± 0.53 s vs. 21.86 ± 0.54 s, p = 0.016) and ACE II (21.33 ± 0.56 vs. 21.93 ± 0.67 sec, p = 0.004) counterparts and only one case of ACE II, and no cases of ACTN3 577XX, had a faster 200-m time than the 2012 London Olympics qualifying (vs. 12 qualified sprinters with 577RR or 577RX gen...
The C34T genetic polymorphism (rs17602729) in the AMPD1 gene, encoding the skeletal muscle-specific isoform of adenosine monophosphate deaminase (AMPD1), is a common polymorphism among Caucasians that can impair exercise capacity. The aim...
moreThe C34T genetic polymorphism (rs17602729) in the AMPD1 gene, encoding the skeletal muscle-specific isoform of adenosine monophosphate deaminase (AMPD1), is a common polymorphism among Caucasians that can impair exercise capacity. The aim of the present study was twofold: (1) to determine the C34T AMPD1 allele/genotype frequency distributions in Lithuanian athletes (n = 204, stratified into three groups: endurance, sprint/power and mixed) and compare them with the allele/genotype frequency distributions in randomly selected healthy Lithuanian non-athletes (n = 260) and (2) to compare common anthropometric measurements and physical performance phenotypes between the three groups of athletes depending on their AMPD1 genotype. The results of our study indicate that the frequency of the AMPD1 TT genotype was 2.4% in the control group, while it was absent in the athlete group. There were significantly more sprint/power-orientated athletes with the CC genotype (86.3%) compared with the en...
The aim of this study was to determine the impact of ACE (I/D), PPARGC1A (G/A) and PPARA (G/C) polymorphisms on footballers performance among 199 Lithuanian professional footballers and 167 sedentary, healthy men (controls). Genotyping...
moreThe aim of this study was to determine the impact of ACE (I/D), PPARGC1A (G/A) and PPARA (G/C) polymorphisms on footballers performance among 199 Lithuanian professional footballers and 167 sedentary, healthy men (controls). Genotyping was performed using polymerase chain reaction and restriction fragment length polymorphism methods on DNA from leucocytes. Results revealed that the angiotensin-1-coverting enzyme gene (ACE) genotype distribution was significantly different between total football players group (II 23.6%, ID 46.7% and DD 29.6%) and the controls (II 24.6%, ID 29.9% and DD 45.5%; P=0.002). Although investigating PPARGC1A (G/A) and PPARA (G/C) polymorphisms no significant results were obtained in the total football players group, however, significant differences were determined between forwards and controls [PPARGC1A: GG 54.6%, GA 29.5%, AA 15.9% vs. GG 49.7%, GA 44.3% and AA 6.0% (P = 0.044); PPARA: GG 52.3%, GC 40.9%, CC 6.8% vs. GG 72.4%, GC 24.6% and CC 3.0% (P = 0.034)]. In the whole cohort, the odds ratio of the genotype [ACE ID + PPARA GG] being a footballer was 1.69 (95% CI 1.04-2.74), and of [ACE ID + PPARGC1A GG] 1.93 (95% CI 1.10-3.37) and of [ACE II + PPARA GC] 2.83 (95% CI 1.02-7.91) compared to controls. It was revealed that ACE ID genotype together with PPARA GG and PPARGC1A GG as well as ACE II genotype with PPARA GC is probably the &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;preferable genotype&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; for footballers. Summing up, the present study suggests that the ACE, PPARGC1A and PPARA polymorphisms genotypes are associated, separately and in combination, with Lithuanian footballers&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; performance.