magdi eldamarawi
Tanta University, Physiology, Faculty Member
Research Interests:
Background: Osteoarthritis (OA) is a common joint disease influencing the majority of individuals over the age of 65. The most affected joints in OA are knees, hands, hips, and spine leading to impaired mobility in the elderly.... more
Background: Osteoarthritis (OA) is a common joint disease influencing the majority of individuals over the age of 65. The most affected joints in OA are knees, hands, hips, and spine leading to impaired mobility in the elderly. Objectives: To analyze and correlate the level of plasma adiponectin and the adiponectin G/T (rs1501299) single nucleotide polymorphism in a group of Egyptians with knee osteoarthritis (OA) and a control group. Materials and methods: 130 unrelated subjects with knee OA and 120 healthy subjects were enrolled in this study. Peripheral blood was used for extraction of DNA which was used to genotype adiponectin gene G/T (rs1501299) polymorphism utilizing polymerase chain reaction followed by digestion with restriction endonuclease. Plasma adiponectin level was estimated using enzyme linked immunosorbent assay (ELISA). Results: Plasma adiponectin concentration was higher in the OA subjects than the controls (P = 0.01). For the adiponectin G/T (rs1501299) polymorph...
Research Interests:
BACKGROUND: Uncoupling proteins (UCP) and β3-adrenergic receptor (ADRB3) gene polymorphism have been implicated in the susceptibility to type 2 diabetes mellitus (T2DM) but the results are inconsistent and inconclusive. AIM: The aim was... more
BACKGROUND: Uncoupling proteins (UCP) and β3-adrenergic receptor (ADRB3) gene polymorphism have been implicated in the susceptibility to type 2 diabetes mellitus (T2DM) but the results are inconsistent and inconclusive. AIM: The aim was to investigate the relationship between -3826A/G (rs1800592) of UCP 1 and ADRB3 Trp64Arg (C/T) (rs4994) gene polymorphism and the incidence of T2DM among Saudis. METHODS: Genotyping of both UCP1 and ADRB3 genes was carried out in 110 healthy controls and 108 unrelated Saudis with T2DM using polymerase chain reaction-based restriction fragment length polymorphism. RESULTS: The genotype and allele frequencies of the UCP1 gene did not differ significantly between diabetic subjects and controls (p > 0.05). However, the CT genotype and the T allele of ADRB3 were higher in diabetic subjects compared with the controls while the CC genotype and C allele were higher in the controls relative to the diabetic subjects (Odds ratios [OR]: 7.85, 95% confidence i...
Research Interests:
Research Interests:
Coronary artery disease (CAD) represents a leading cause of morbidity and mortality worldwide, and genetic factors contribute to the development of this disease. We conducted a case-control study to assess the association between... more
Coronary artery disease (CAD) represents a leading cause of morbidity and mortality worldwide, and genetic factors contribute to the development of this disease. We conducted a case-control study to assess the association between interleukin 17A (IL17A) rs2275913 and rs3748067 polymorphisms and development of CAD. A total of 372 CAD patients and 372 healthy controls were recruited in our investigation between January 2013 and December 2014. Genotyping of IL17A rs2275913 and rs3748067 was carried out using polymerase chain reaction combined with restriction fragment length polymorphism. Logistic regression analysis revealed that CC [odds ratio (OR) = 3.81, 95% confidence interval (CI) = 2.11-7.16] and TC+CC (OR = 1.54, 95%CI = 1.11-2.14) rs3748067 genotypes were associated with an increased risk of CAD compared to the TT variant. Individuals carrying the TC+CC genotype were more likely to have a higher risk of CAD if they were smokers, with an adjusted OR (and 95%CI) of 2.20 (1.31-3....
Research Interests:
Research Interests:
AIM: This research was designed to study the effect of chronic administration of obestatin on serum testosterone level in obese male rats. METHODS: This study was performed on 40 male albino rats, weighed 200-250 grams. Ten rats were used... more
AIM: This research was designed to study the effect of chronic administration of obestatin on serum testosterone level in obese male rats. METHODS: This study was performed on 40 male albino rats, weighed 200-250 grams. Ten rats were used as a control group (group I) and the rest were made obese by feeding them a hypercaloric diet for 15 weeks. The obese rats were randomly divided into two groups, group II in which the rats received the hypercaloric diet only and group III which is subdivided into two subgroups a and b. In group III, the obese rats are kept on the hypercaloric diet with intraperitoneal administration of obestatin in a dose of 16 nmol/kg/day for two and four weeks in group IIIa and IIIb respectively. RESULTS: By checking the results of the different groups we noticed that obesity, in group II, produced significant increase in the body weight of the rats associated with significant decrease in the serum testosterone level. In group IIIa, administration of obestatin fo...
Background/Aim: The increased level of advanced glycation end products (AGEs) in diabetic patients is considered one of the most important factors responsible for the development of diabetic complications. This study was designed to... more
Background/Aim: The increased level of advanced glycation end products (AGEs) in diabetic patients is considered one of the most important factors responsible for the development of diabetic complications. This study was designed to compare the effect of regular moderate exercise and pyridoxamine on the serum level of advanced glycation products in type 2 diabetic rats. Material & methods: This study was performed on 40 male albino rats; weighed 200-250 g. Ten rats were separated and used as a control group (group I). The other 30 rats were prepared to develop diabetes mellitus by a single intravenous injection of 45 mg/kg streptozotocin (STZ). Three days after injection of streptozotocin, fasting blood glucose was determined and animals with fasting blood glucose level more than 200 mg/dl were considered to have diabetes. Diabetic rats were divided into three groups: group II (diabetic group), group III (exercise group) and group IV (pyridoxamine group). Each group consisted of 10 ...
Background/Aim: This work was done to study the combined effect of diet restriction and moderate regular exercise on plasma adiponectin level and expression of its receptors (AdipoR1) in skeletal muscles and their role in the amelioration... more
Background/Aim: This work was done to study the combined effect of diet restriction and moderate regular exercise on plasma adiponectin level and expression of its receptors (AdipoR1) in skeletal muscles and their role in the amelioration of metabolic disturbances associated with obesity and type 2 diabetes mellitus. Materials & Methods: The study included 50 male Wistar albino rats weighed 150- 200 g, divided into 5 groups: control (group I), obese-diabetic (group II), obese-diabetic with diet restriction (group III), obese-diabetic with exercise (group IV) and obese-diabetic with diet restriction and exercise (group V). At the end of the study, body weight was detected, retro-orbital blood samples were analyzed for blood glucose, plasma triglycerides (TG), total cholesterol (TC), plasma insulin and adiponectin levels and insulin resistance was assessed. Also, mRNA level of AdipoR1 in skeletal muscles was measured. RESULTS: Comparing the results of group II (obese diabetic) to that...
This study was designed to investigate the effects of combination of antioxidants (Vitamins C and E) on endothelial inflammation biomarkers (proinflammatory cytokines and endothelial adhesion molecules) and oxidative stress in liver and... more
This study was designed to investigate the effects of combination of antioxidants (Vitamins C and E) on endothelial inflammation biomarkers (proinflammatory cytokines and endothelial adhesion molecules) and oxidative stress in liver and kidney in streptozotocin-induced diabetic rats. Thirty male albino rats, weighing between 170 and 200 g were used. The animals were divided into three groups as follows; control non diabetic rats (group I), untreated diabetic rats (group II), and diabetic rats treated with vitamins C and E (group III). Diabetes was developed 3 days after intraperitoneal administration of a single 40 mg/kg dose of streptozotocin in groups II and III. Thereafter, the rats in group III began to receive a combination of vitamins C and E for four weeks. The rats in group II had significantly higher levels in plasma glucose, total cholesterol (TC), triglycerides (TG), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 (ICAM-1) and vasc...
ABSTRACT
Research Interests:
Research Interests: Insulin()
()
Research Interests:
Research Interests:
AIM: This study was designed to compare the impact of two different exercise protocols on the serum level of advanced glycation end products (AGEs) in type 2 diabetic rats. METHODS: This study was performed on 40 male albino rats, weighed... more
AIM: This study was designed to compare the impact of two different exercise protocols on the serum level of advanced glycation end products (AGEs) in type 2 diabetic rats. METHODS: This study was performed on 40 male albino rats, weighed 200-250 g. At the start of the study, 10 male rats were separated and used as a control group (group I). Induction of diabetes in another 30 rats was done by a single intravenous injection of 45 mg/kg streptozotocin (STZ). The diabetic rats were randomly divided into two groups: group II (sedentary group) (n = 10) and group III (exercised group) (n = 20). Then, the exercised group was further subdivided into two subgroups: IIIa (were subjected to chronic regular moderate exercise protocol for 8 weeks and IIIb faced another protocol in the form of irregular strenuous exercise for the same period. RESULTS: The data in group II (sedentary group) as compared to group I (control group), showed that the injection of streptozotocin resulted in significant increase in the fasting blood glucose, serum AGEs and insulin levels and HOMA-IR. Also, there was significant decrease in the extracellular superoxide dismutase (SOD) enzyme serum level. Meanwhile, in subgroup IIIa (as compared to group II), regular moderate exercise protocol in diabetic rats produced significant reduction in the fasting blood glucose, serum AGEs and insulin levels and HOMA-IR. At the same time, there was significant increase in serum level of superoxide dismutase. Lastly, in subgroup IIIb (as compared to group II), the irregular intense exercise protocol in diabetic rats caused significant reduction in fasting blood glucose but there was insignificant decrease in serum AGEs and insulin levels and HOMA-IR. This was associated with insignificant increase in the superoxide dismutase serum level. CONCLUSION: In type 2 diabetes, regular moderate exercise protocol is more valuable program to reduce serum level of advanced glycation end products than irregular severe one. This regular moderate protocol will be very helpful in the prevention of development of diabetic complications. [Mahmoud E. Salama, Magdi A. El-Damarawi and Afrah F. Salama. A Comparison between the Impact of Two Different Exercise Protocols on Advanced Glycation End Products in Type 2 Diabetic Rats. Life Sci J 2013;10(3):860-869] (ISSN: 1097-8135). http://www.lifesciencesite.com. 129