Cellulosic biomass-hydrolyzing enzymes

SK Sandhu, A Mathur, R Gupta, SK Puri, M Adsul - Waste to wealth, 2018 - Springer
Biomass is a heterogeneous matrix of three interlinked polymers of cellulose–hemicellulose–
lignin. Polymers such as cellulose and hemicellulose need to be fractioned and converted …

Global and grain‐specific accumulation of glycoside hydrolase family 10 xylanases in transgenic maize (Zea mays)

BN Gray, O Bougri, AR Carlson… - Plant biotechnology …, 2011 - Wiley Online Library
In planta expression of cell wall degrading enzymes is a promising approach for developing
optimized biomass feedstocks that enable low‐cost cellulosic biofuels production …

TrypZean™: An animal-free alternative to bovine trypsin

A Krishnan, SL Woodard - Commercial Plant-Produced Recombinant …, 2014 - Springer
Trypsin derived from either porcine or bovine pancreas is widely used in biopharmaceutical
manufacturing. This industry is working toward replacing animal source materials with …

Biophysical and biochemical studies of a major endoglucanase secreted by Xanthomonas campestris pv. campestris.

FR Rosseto, LR Manzine, M de Oliveira Neto… - Enzyme and Microbial …, 2016 - Elsevier
Endoglucanases are the main cellulolytic enzymes secreted by the bacterium Xanthomonas
campestris pv. campestris (Xcc). The major endoglucanase exported by this bacterium into …

Seed-based production system for molecular farming

F Takaiwa - Tools & Techniques of Plant Molecular Farming, 2023 - Springer
Seed is an ideal bioreactor for the expression of high-value recombinant proteins. High
production can be achieved by taking advantage of established seed-specific promoters and …

Enhanced production of reducing sugars from transgenic rice expressing exo-glucanase under the control of a senescence-inducible promoter

K Furukawa, S Ichikawa, M Nigorikawa, T Sonoki… - Transgenic …, 2014 - Springer
We generated transgenic rice plants that express EXG1 exo-glucanase under the control of
a senescence-inducible promoter. When a GUS coding sequence was connected to a …

Synergistic effects of 2A-mediated polyproteins on the production of lignocellulose degradation enzymes in tobacco plants

DS Lee, KH Lee, S Jung, EJ Jo… - … of experimental botany, 2012 - academic.oup.com
Cost-effective bioethanol production requires a supply of various low-cost enzymes that can
hydrolyse lignocellulosic materials consisting of multiple polymers. Because plant-based …

In planta differential targeting analysis of Thermotoga maritima Cel5A and CBM6-engineered Cel5A for autohydrolysis

SA Mahadevan, SG Wi, YO Kim, KH Lee, HJ Bae - Transgenic research, 2011 - Springer
The heterologous expression of glycosyl hydrolases in bioenergy crops can improve the
lignocellulosic conversion process for ethanol production. We attempted to obtain high-level …

Developing cellulolytic organisms for consolidated bioprocessing of lignocellulosics

WH van Zyl, R den Haan, DC la Grange - Biofuel Technologies: Recent …, 2013 - Springer
Lignocellulosic biomass represents an abundant, renewable feedstock for the production of
biofuels and chemicals in a potentially sustainable manner. The main reason that it is not …

Bipartite and tripartite Cucumber mosaic virus-based vectors for producing the Acidothermus cellulolyticus endo-1,4-β-glucanase and other proteins in non …

MS Hwang, BE Lindenmuth, KA McDonald, BW Falk - Bmc Biotechnology, 2012 - Springer
Background Using plant viruses to produce desirable proteins in plants allows for using non-
transgenic plant hosts and if necessary, the ability to make rapid changes in the virus …