In planta differential targeting analysis of Thermotoga maritima Cel5A and CBM6-engineered Cel5A for autohydrolysis

SA Mahadevan, SG Wi, YO Kim, KH Lee, HJ Bae - Transgenic research, 2011 - Springer
The heterologous expression of glycosyl hydrolases in bioenergy crops can improve the
lignocellulosic conversion process for ethanol production. We attempted to obtain high-level …

High level expression of Acidothermus cellulolyticusβ-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid

HL Chou, Z Dai, CW Hsieh, MSB Ku - Biotechnology for biofuels, 2011 - Springer
Background Large-scale production of effective cellulose hydrolytic enzymes is the key to
the bioconversion of agricultural residues to ethanol. The goal of this study was to develop a …

Optimization of Acidothermus cellulolyticus Endoglucanase (E1) Production in Transgenic Tobacco Plants by Transcriptional, Post-transcription and Post-translational …

Z Dai, BS Hooker, RD Quesenberry, SR Thomas - Transgenic Research, 2005 - Springer
An attempt was made to obtain a high-level production of intact Acidothermus cellulolyticus
endoglucanase (E1) in transgenic tobacco plants. The E1 expression was examined under …

Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants

GCG Biswas, C Ransom, M Sticklen - Plant Science, 2006 - Elsevier
Commercial production of ethanol from plant biomass sources employs enzymatic
hydrolysis of cellulose to glucose. Transgenic plants that can produce their own hydrolysis …

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize

R Brunecky, MJ Selig, TB Vinzant, ME Himmel… - Biotechnology for …, 2011 - Springer
The glycoside hydrolase family 5 endocellulase, E1 (Cel5A), from Acidothermus
cellulolyticus was transformed into both Nicotiana tabacum and Zea mays with expression …

Heterologous Acidothermus cellulolyticus 1,4-β-Endoglucanase E1 Produced Within the Corn Biomass Converts Corn Stover Into Glucose

C Ransom, V Balan, G Biswas, B Dale… - … April 30–May 3, 2006, in …, 2007 - Springer
Commercial conversion of lignocellulosic biomass to fermentable sugars requires
inexpensive bulk production of biologically active cellulase enzymes, which might be …

Production of Gloeophyllum trabeum Endoglucanase Cel12A in Nicotiana benthamiana for Cellulose Degradation

KR Geem, Y Song, I Hwang, HJ Bae… - Frontiers in Plant …, 2021 - frontiersin.org
Lignocellulosic biomass from plants has been used as a biofuel source and the potent acidic
endoglucanase GtCel12A has been isolated from Gloeophyllum trabeum, a filamentous …

Improved recombinant cellulase expression in chloroplast of tobacco through promoter engineering and 5′ amplification promoting sequence

S Jung, DS Lee, YO Kim, CP Joshi, HJ Bae - Plant molecular biology, 2013 - Springer
Economical production of bioethanol from lignocellulosic biomass still faces many technical
limitations. Cost-effective production of fermentable sugars is still not practical for large-scale …

Expression of glycosyl hydrolases in lignocellulosic feedstock: an alternative for affordable cellulosic ethanol production

Y Xiao, C Poovaiah, HD Coleman - BioEnergy Research, 2016 - Springer
Ethanol produced from lignocellulosic feedstock is a promising alternative to fossil fuels and
corn-sourced ethanol. However, it creates unique challenges in terms of requirements for …

Synergistic effects of 2A-mediated polyproteins on the production of lignocellulose degradation enzymes in tobacco plants

DS Lee, KH Lee, S Jung, EJ Jo… - … of experimental botany, 2012 - academic.oup.com
Cost-effective bioethanol production requires a supply of various low-cost enzymes that can
hydrolyse lignocellulosic materials consisting of multiple polymers. Because plant-based …