Eukaryotes characteristically express a cytochrome P450-catalyzed sterol 14α-methyl
demethylase as an essential step in the production of membrane sterols. Lanosterol 14α …

Abstract Cytochrome P450 sterol 14-demethylase (P450-CYP51) is the enzyme that
catalyzes 14α demethylation of lanosterol, a step in ergosterol biosynthesis, on the …

Sterol biosynthesis requires the removal of the 14α-methyl group from lanosterol in animals
and fungi and from obtusifoliol in plants. This reaction is catalyzed by a microsomal …

Lanosterol 14α-demethylase (CYP51F1) from Candida albicans is known to be an essential
enzyme in fungal sterol biosynthesis. Wild-type CYP51F1 and several of its mutants were …

Sterols are isoprenoid-derived lipids that are produced via the mevalonate pathway and are
involved in various cellular functions in eukaryotes such as maintenance of membrane …

Summary Interaction of obtusifoliol and 24, 28-dihydroobtusifoliol with yeast lanosterol 14a-
demethylase (P-450 14dm) was studied to elucidate the role of the 4β-methyl group of …

P450 was purified from microsomal fractions of a strain of Saccharomyces cerevisiae which
contained detectable P450 despite the disruption of CYP51A1. The P450 had a molecular …

Metabolism of lanosterol (LAN), 24‐methylene‐24, 25‐dihydrolanosterol (24‐
methyleneDHL), dihydrolanosterol (DHL) and obtusifoliol (OBT) by purified human, plant …

The identification of the precise structural features of yeast sterol molecules required for the
essential “sparking” function has been a controversial area of research. Recent cloning and …

The ERG5 gene from Saccharomyces cerevisiae was cloned by complementation of an erg5-
1 mutation using a negative selection protocol involving screening for nystatin-sensitive …