Evaluating a multigene environmental DNA approach for biodiversity assessment

AJ Drummond, RD Newcomb, TR Buckley, D Xie… - …, 2015 - academic.oup.com
Background There is an increasing demand for rapid biodiversity assessment tools that
have a broad taxonomic coverage. Here we evaluate a suite of environmental DNA (eDNA) …

The importance of molecular markers and primer design when characterizing biodiversity from environmental DNA

JR Freeland - Genome, 2017 - cdnsciencepub.com
Environmental DNA (eDNA) comprises DNA fragments that have been shed into the
environment by organisms, and which can be extracted from environmental samples such …

[HTML][HTML] Executing multi-taxa eDNA ecological assessment via traditional metrics and interactive networks

M Seymour, FK Edwards, BJ Cosby, MG Kelly… - Science of the Total …, 2020 - Elsevier
Current approaches to ecological assessment are limited by the traditional morpho-
taxonomic methods presently employed and the inability to meet increasing demands for …

Revisiting the effect of PCR replication and sequencing depth on biodiversity metrics in environmental DNA metabarcoding

S Shirazi, RS Meyer, B Shapiro - Ecology and Evolution, 2021 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding is an increasingly popular tool for measuring
and cataloguing biodiversity. Because the environments and substrates in which DNA is …

Effects of sampling effort on biodiversity patterns estimated from environmental DNA metabarcoding surveys

EK Grey, L Bernatchez, P Cassey, K Deiner… - Scientific Reports, 2018 - nature.com
Environmental DNA (eDNA) metabarcoding can greatly enhance our understanding of
global biodiversity and our ability to detect rare or cryptic species. However, sampling effort …

[BOOK][B] A practical guide to DNA-based methods for biodiversity assessment.

K Bruce, RC Blackman, SJ Bourlat, M Hellström… - 2021 - repository.oceanbestpractices.org
This book represents a synthesis of knowledge and best practice in the field of DNA-based
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …

Making environmental DNA (eDNA) biodiversity records globally accessible

O Berry, S Jarman, A Bissett, M Hope… - Environmental …, 2021 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding, where specific DNA markers (barcodes) are
purified from the environment, sequenced, and used to identify organisms, is revolutionizing …

[HTML][HTML] Environmental DNA–An emerging tool in conservation for monitoring past and present biodiversity

PF Thomsen, E Willerslev - Biological conservation, 2015 - Elsevier
The continuous decline in Earth's biodiversity represents a major crisis and challenge for the
21st century, and there is international political agreement to slow down or halt this decline …

An efficient and robust laboratory workflow and tetrapod database for larger scale environmental DNA studies

J Axtner, A Crampton-Platt, LA Hörig, A Mohamed… - …, 2019 - academic.oup.com
Background The use of environmental DNA for species detection via metabarcoding is
growing rapidly. We present a co-designed lab workflow and bioinformatic pipeline to …

DNA from soil mirrors plant taxonomic and growth form diversity

NG Yoccoz, KA Bråthen, L Gielly, J Haile… - Molecular …, 2012 - Wiley Online Library
Ecosystems across the globe are threatened by climate change and human activities. New
rapid survey approaches for monitoring biodiversity would greatly advance assessment and …