RU2811227C1 - Antibacterial hand spray - Google Patents

Abstract

FIELD: cosmetic personal hygiene products.

SUBSTANCE: invention can be used in the production of products for the hygienic treatment of hand skin. The antibacterial hand treatment product includes the following components, wt.%: 25.0–30.0 of mountain savory hydrolate, 23.0–25.0 of coriander hydrolate, 22.0–25.0 of clary sage hydrolate, up to 100% of Crimean angustifolia lavender hydrolate.

EFFECT: resulting product has antibacterial, moisturizing and wound-healing effects and provides high-quality protection and cleansing of the skin of the hands from bacterial contamination, has an aqueous consistency and is well absorbed into the skin.

1 cl, 3 dwg, 2 ex

Description

The invention relates to personal hygiene cosmetics and can be used in the production of products for the hygienic treatment of hand skin, and is a composition based on plant raw materials with antibacterial, moisturizing and wound-healing effects to effectively protect and clean hands from bacterial contamination.

The spread of antibiotic-resistant strains of microorganisms and the deterioration of the environmental situation are having an increasingly strong impact on human health. Hand hygiene is the most cost-effective and effective method of preventing the spread of infections. The consequences of contact transmission of infection can be complications in the form of chronic diseases and even death. Currently, there is a huge range of antiseptic drugs with antimicrobial, antiviral, antiparasitic, antifungal effects, used primarily for external use for the prevention and treatment of infectious diseases. Antiseptics inhibit the activity of many bacterial enzymes and, under their influence, the process of cell division stops and morphological changes occur, accompanied by a violation of the cellular structure.

Quite stringent requirements are imposed on antiseptics, in particular, they, along with high antimicrobial activity, a wide spectrum of antimicrobial action and good organoleptic properties, should not have a toxic, organotropic, allergic, mutagenic, oncogenic, teratogenic and irritating effect on the body, as well as production antiseptics must be economically feasible. A modern disinfectant should be a composition based on a balanced formula, including one or more active substances in ratios that allow for maximum synergy or potentiation of the effect in the ratio of the most resistant microorganisms, as well as functional additives that purposefully change their properties.

However, as part of antiseptic agents, powerful chemical compounds of antifungal, antimicrobial, antiviral nature are often used, which are capable of penetrating into the deep layers of the skin, accumulating in internal organs and tissues, and being detected in the blood serum, thereby causing significant harm to the entire body as a whole. Some strong antiseptics can cause chemical burns or severe irritation when applied to the skin without dilution with water, and even diluted antiseptics can cause irritation if left on the skin for a long time, which can lead to contact dermatitis.

The hand sanitizer composition is typically a mixture of components, said composition including an antimicrobial agent. The antimicrobial agent may be benzalkonium chloride or triclosan, but most often the antimicrobial agent is one of the alcohols, most commonly ethanol. In addition, such compositions have a strong unpleasant odor, which makes their use in children and pregnant women difficult. Sometimes other antiseptic substances, such as benzalkonium, are added to alcohol to enhance the effect. Such products are more effective, but can dry out the skin more and cause irritation.

The addition of various thickeners that transform the solution into a gel makes the product more convenient to use, but does not affect the antimicrobial activity, just as other additives do not affect it: fragrances, glycerin, dexpanthenol. All this is necessary only for a pleasant smell or moisturizing of the skin. When the gel dries, only the alcohol evaporates, the gel base remains on the hands and can create an unpleasant feeling of stickiness, especially with repeated use of the product, and the gel base itself does not kill microbes, that is, after the alcohol has evaporated, the antiseptic effect of the product stops.

Hands must be treated with alcohol antiseptics for at least 30 seconds, which increases the consumption of the product, and if the hands are contaminated with fat or protein, the effectiveness of the antiseptic is greatly reduced. Continuous use of these hand sanitizers can cause dry skin, resulting in red, cracked, and flaky hands.

The urgency of the problem of creating new antimicrobial agents is determined by the colossal adaptive ability of microorganisms, the huge number of biological forms of pathogens, the constant emergence of forms with multiple resistance, the emergence of new types of microorganisms that make the treatment and prevention of infectious diseases difficult.

The development of antibacterial hand sanitizers containing ingredients that are safe to use and have antibacterial, antifungal, antiviral, and anti-inflammatory properties is very relevant.

In this regard, herbal medicines, which are topical agents that include plant components containing complexes of biologically active substances that are close to the body’s metabolites and are biologically compatible with them, are becoming increasingly common.

Fractions from plant raw materials are natural antiseptics with a wide range of positive effects and have a number of advantages over other agents, since their properties are provided by multicomponent compounds, many of which are close in chemical structure and biological action to endogenous compounds involved in the bioregulation of the physiological systems of the macroorganism. The mildness of the action of herbal medicines, the use of low doses of herbal components in them, good tolerability, which makes it possible to practically eliminate side effects on the human body during their use, the absence of toxic manifestations during long-term use, the absence of contraindications for use and a pronounced bioregulatory effect - all this is their advantage over other known drugs, and provides the possibility of individual and mass use.

An antibacterial agent for treating skin is known (RF Patent No. 2185157, class A 61 K 31/14, 2000), containing a modified cellulose derivative, the antiseptic miramistin and water.

However, Miramistin used in the antiseptic does not have a prolonged effect on the skin of the hands and is effective only for a narrow area of use.

An antibacterial agent for skin is known (RF patent No. 2167650, IPC A61K 9/10, 9/06, 2001, Bulletin No. 15), containing microparticles of colloidal silver, essential oils of monarda and tea tree, enclosed in a lipid component and distributed in a gel medium with preservatives, plant extracts, essential oils and stabilizing additives.

The disadvantages of the product are the following: the use of only monarda essential oil and only as an auxiliary component that enhances the effect of silver, and in one concentration, significantly reduces the effectiveness against many microorganisms that usually make up the population of pathogens; The absence of other active substances from monarda in the product, in particular flavonoids, limits the scope of application and does not allow its use as a fungicidal, antimicrobial, or virucidal.

An antibacterial hand treatment agent is known (Patent RU 2342923, published on January 10, 2009), including 1,2-propylene glycol, ethyl alcohol or isopropanol, glycerin, carbopol ultrez 20, triethanolamine and water in certain proportions, which is a non-toxic, stable gel, which contains fragrance, dyes, vitamins and biologically active additives.

The disadvantages of this known product are the low speed of action of the disinfectant, as well as the use of dyes, which can negatively affect the epidermis of the skin of the hands, the increased content of glycerol, which is a fatty polyhydric alcohol, and an oily liquid, which at high concentrations leaves an oily film on the hands and can contaminate workers surfaces, in addition, the known product is not universal, has low consumer qualities, low homogeneity of the base, and, being a dispersed mixture, is subject to stratification, sedimentation and other processes of microphase separation, impossibility of use in children due to the alcohol content in the composition.

An antibacterial agent for skin treatment is known (RU 2465012, publ. October 27, 2012), containing isopropanol, polyhexamethylene biguanide hydrochloride, alkyldimethylbenzylammonium chloride, propylene glycol, calendula extract, glycerin, fragrance and demineralized water.

The disadvantages of this product are the possibility of developing adverse reactions on the skin of the hands; the product is not universal and does not have a wide spectrum of action.

An antibacterial agent for treating the skin of hands is known (RF Patent 2535031, publ. December 10, 2014), containing octenidine dihydrochloride, propanol-1, propanol-2, citric acid, lavender essential oil and softened water in the following ratio of components, in wt.%: octenidine dihydrochloride 0.01-0.2, propanol-1 - 20, propanol-2 - 50, citric acid - 0.001-0.002, lavender essential oil - 0.2-0.5, softened water.

The disadvantages of this product are the following: the presence of alcohols in the composition can have a provoking undesirable side effect, namely, local irritation of the skin of the hands and dry skin, the known product, which is a solution in the form of a transparent colorless liquid, has an alcoholic odor, the components used in the composition of the product increase the risk of allergic manifestations on the skin, which makes it undesirable for use in children, pregnant women,

An antimicrobial agent for the skin is known (RF Patent No. 2179456 A61L 26/00, 15/58, 2002, Bulletin No. 5), containing aqueous-alcoholic extracts from the leaves of eucalyptus or propolis, or the buds of various types of poplar, or the herb Echinacea purpurea, or the fruits of milk thistle, or the flowers of lavender, or the leaves of sage, or the buds of silver birch, or the flowers of calendula, or the herb of lemon balm, or the roots of licorice, or the bark of common lilac, or the rhizomes of Rhodiola rosea, or the herb of wormwood tarragon, or herb monarda fistula - 7.8 - 39.0, fish collagen - 3.9 - 39.06, medical glycerin - 0.39 - 3.52 dimethyl sulfoxide - 0.195 - 0.98, purified water - up to 100.0, at appropriate ratio of components.

The disadvantages of this known product are the following: the use of dimethyl sulfoxide as a penetrator can cause local skin irritation and disruption of the protective lipid barrier; the product does not have the ability to completely destroy viruses and spores of bacilli, clostridia, fungi or exhibits this ability to an insufficient extent, has an unpleasant odor and stickiness; may increase the risk of allergic skin reactions; a high alcohol content in the composition can cause undesirable side effects, namely dry skin, itchy skin, which narrows the range of its use, namely in children and pregnant women.

An antibacterial product for the skin is known (RF Patent 2626673, published on July 31, 2017), which is a hydrolate of Gynura Procumbens leaves, obtained by steam or water distillation of fresh or dry leaves of Gynura Procumbens at a ratio of fresh leaves and water of 1: 3-6 or at a ratio of dry leaves and water 1:30-60.

The disadvantages of the known product are the following: insufficiently expressed antibacterial activity when treating the skin of the hands, limited range of effects, rare and inaccessible raw materials.

An antibacterial product for treating the skin of hands (Patent 2523560, published on July 20, 2014) was selected as a prototype, including ethyl alcohol, glycerin, carbopol ultras, softened water, aminomethylpropanol, essential oil selected from the group: eucalyptus essential oil, lemon essential oil, mint essential oil, anise essential oil, jasmine essential oil with the following ratio of components in g per 100 g of gel: ethyl alcohol 65.0-66.0; glycerin 1.0-2.0; carbopol ultrez 0.16-0.18; aminomethylpropanol 0.06-0.08; essential oil 0.005-0.03; softened water - up to 100.0.

The disadvantages of the known product are the following: insufficient antibacterial activity, multicomponent composition can increase the risk of allergic manifestations on the skin and other side effects, complicates its preparation, limited use of the product due to the high alcohol content in the composition, which can have a provoking negative effect, namely cause dry skin, skin irritation, which makes it undesirable for use in children and pregnant women.

A patent information search showed that individual components of an antibacterial agent or their combinations are found in known medicinal products for similar purposes, but antibacterial agents were not identified in the proposed set of ingredients and their ratio in the claimed composition.

Based on the above level of technology, when creating antibacterial hand treatment products, the problem is to achieve a multifaceted effect, namely antiseptic, anti-inflammatory, regenerating and moisturizing, as well as a pronounced prolonged action, ensuring maximum effectiveness without the development of adverse reactions, especially in children and pregnant women , women during lactation and patients with chronic diseases.

Development of a new antibacterial hand treatment based on a complex of biologically active substances in the form of a combination of four hydrolates of medicinal plants due to a synergistically selected composition of ingredients, providing increased antibacterial activity of the product while maintaining the stability of the composition throughout the entire shelf life, and at the same time having a high speed of action against pathogens, does not cause the risk of addiction, does not contain artificial preservatives, alcohols, emulsifiers, flavors and dyes, which reduces the toxicity of the effect, helps soften and moisturize the skin, and also stimulates skin regeneration, will solve the above problem.

The technical result consists in creating an antibacterial hand treatment product with increased bactericidal properties, which has anti-inflammatory and regenerative effects, does not cause allergic reactions, and is safe for treating the skin.

An additional result is the expansion of the range of domestic antibacterial products for hand treatment, which do not have a pronounced irritating side effect, do not contain alcohols and emulsifiers, have a long shelf life and are easy to use. All components used in the product are not scarce, are available, and can be introduced into industrial production. The absence of alcohol and synthetic components in the product makes this product safe for use in children and pregnant women.

The antibacterial agent was developed by the authors for the first time and made it possible to obtain a new composition for effective and high-quality treatment of hand skin with a prolonged action, which does not contain artificial preservatives, alcohols, emulsifiers, flavors and dyes, but at the same time has high biocidal activity with simultaneous regenerating and moisturizing effects, and having a wide range of effects due to the unique composition of biologically active substances included in its composition.

It is proposed, as in the prototype, to use phytoextracts of medicinal plants in the composition.

The creation of a complex hand treatment product with high antimicrobial activity based on a full set of biologically active substances contained in hydrolates of medicinal plants allows us to expand the range of environmentally friendly, highly effective compositions based on natural compounds - phytoextracts, which have pronounced activity against pathogenic microorganisms and at the same time have anti-inflammatory, regenerating properties. The use of the proposed product ensures the prevention of infections of microbial etiology.

The essence of the claimed invention is that the antibacterial product for treating the skin of the hands, including phytoextracts of medicinal plants, according to the invention, as phytoextracts contains mountain savory hydrolate, coriander hydrolate, clary sage hydrolate and Crimean lavender hydrolate in the following ratio of components, wt. %:

Mountain savory hydrosol 25.0-30.0 Coriander hydrolate 23.0-25.0 Clary sage hydrolate 22.0-25.0 Hydrolate of Crimean lavender angustifolia the rest up to 100%

The cause-and-effect relationship between the set of essential features and the technical result provided by the invention is as follows: the use of four medicinal plants in the combination of hydrolates, which have a very mild therapeutic effect on the body, allows the use of an antibacterial agent for treating the skin of both adults and children , even use for newborn babies, pregnant women; excellent for delicate and sensitive skin prone to dryness, inflammation and cracks, as well as problematic teenage skin prone to excessive sebum production and acne; since hydrolates have an acidic pH of 5 to 6.5, they are able to suppress the growth and activity of pathogenic bacteria; in addition to essential oils, hydrolates contain substances that do not remain in essential oils - water-soluble components of plants that remain in water in the form of ions, usually their percentage of water reaches 0.5%, but depending on the plant, this figure may turn out to be and big.

Hydrolate is an intermediate product in the process of obtaining essential oil from a plant using steam or water distillation. During distillation, essential oils are separated from soluble substances - hydrolates or hydrosols. Steam extracts more valuable substances from the plant than water, therefore the content of extracted substances in the hydrolate is much greater than in a regular decoction, while various substances - salts, dyes and others - do not get into the hydrolate, so the effect of the hydrolate is much more effective and harmless than the effect of decoction In terms of its beneficial properties, hydrolate can be compared to the use of the essential oil of the same name, only it has a milder effect. Hydrolates are excellent skin moisturizers; they contain essential oil molecules and penetrate deeply through the dense top layer of skin, reaching the subcutaneous layers.

Hydrolates are obtained by steam distillation of plant materials at a temperature of 100°C, using water that does not contain any impurities. In this case, water is converted into steam, followed by condensation of the steam into distillate. When water vapor passes through plant material, they are saturated with valuable water-soluble components contained in plants: essential oils, acids, bioflavonoids, vitamins and other valuable substances (Gvozdeva V.V., Kuzina M.A. Properties of hydrolates and technology for their production. International school scientific bulletin. 2020; 2). When using hydrolates, deep and effective hydration of not only the skin, but also the subcutaneous layers is achieved, which promotes the production of healthy cells. As a result, with regular use, the skin returns its natural level of moisture, acquires a healthy color, and the aging process slows down.

The composition of hydrolates is quite complex: flavonoids, carotenoids, phytosterols, pectins and other substances with biologically active effects. The essential oils contained in the hydrolate, in turn, accelerate the division of new cells, increase blood circulation, and have an anti-inflammatory effect, which helps restore elasticity, increase skin immunity, and normalize the functioning of the sebaceous glands.

The use of a mixture of hydrolates of four medicinal plants, which enhance the properties of each other, helps to quickly eliminate microbial contamination; the combination of hydrolates of medicinal plants, which have a pronounced antimicrobial effect against cultures of gram-positive, gram-negative bacteria and fungi of the genus Candida, providing potentiation of the action of the components, expands the area of application points, allows simultaneously with a pronounced antibacterial effect, it also has a biostimulating effect, which consists in restoring the damaged protective barrier of the skin, providing hydration, stimulating regeneration, reducing the inflammatory potential and traumatic effects of external stress.

The components of the antibacterial agent are biocompatible, non-toxic, do not require additional preservatives and stabilizers, have antimicrobial, anti-inflammatory and bacteriostatic effects, contain biologically active substances that inactivate microbes, which ensures high bactericidal activity.

The claimed combination of components of an antibacterial agent in an experimentally selected, optimal quantity ensures the achievement of the claimed result. Other ratios of the taken components do not allow achieving the desired effect in a short time.

As a result of the use of the proposed invention, it becomes possible to expand the range of environmentally safe, highly effective fungicidal, antimicrobial compositions based on natural compounds - phytoextracts of medicinal plants, which have a pronounced inactivating activity against pathogenic microorganisms - viruses, bacteria, fungi, including molds, protozoa, and at the same time having a general strengthening and adaptogenic effect on the human body. The use of the proposed product ensures the prevention of infections of microbial etiology and infections of confined spaces by physiological methods - by influencing pathogenic forms of microorganisms (bacteria, viruses and fungi, including molds) while enhancing the protective properties of the human body.

The inventive product contains hydrolates of mountain savory and coriander as an antibacterial additive, and hydrolates of Crimean angustifolia lavender and clary sage as an anti-inflammatory and wound-healing component.

The selected plant hydrolates are stable, can synergize with skin moisture and other oils, and feel very light, non-greasy, and emollient when applied to the skin.

Mountain savory hydrosol (Satureja montana) contains a large amount of plant phenols and is quite effective against many types of infections. A number of studies of its composition make it possible to identify carvacrol and thymol among the most active components. The high antimicrobial properties of the hydrolate are due to the presence of carvacrol in its composition in an amount of 89.07%. The components of the savory extract doubled the bacteriostatic and bactericidal effect of 40% ethanol against E. coli. The antimicrobial effect of savory essential oil on gram-positive and gram-negative bacteria - Bacillus subtilis, Clostridium perfringens, Micrococcus flavus, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidi, Erwinia amylovora, Salmonella typhi - was noted in the works of a number of researchers (Abolfazl M., Hadi A., Frhad M., Hossein N. In vitro antibacterial activity and phytochemical analysis of some medicinal plants. Journal of Medicinal Plants Research. 2014:8(3):186-194.). Studies have shown that mountain savory fractions have a powerful fungicidal effect against fungal strains Candida albicans, Candida tropicalis, Aspergillus fumigatus, Aspergillus niger, Penicillium sp., Acremonium falciforme, isolated from patients with chronic infectious and inflammatory diseases. At the same time, the components of savory are 12.9 times less toxic compared to amphitericin B (LD50 280 mg/kg) and 6.5 times less toxic than the sodium salt of nystatin (LD50 550 mg/kg) (Ermakova T.S., Priskar V.I., Bodrug M.V., Titov L.P. Research on the antimycotic activity of mountain savory essential oil. Immunopathology, allergology, infectology. 2000:(3):87-88).

Lavender angustifolia hydrolate (Lavandula angustifolia Mill) has a sedative, anticonvulsant, analgesic, antiseptic, and antioxidant effect. The components of angustifolia lavender hydrolate inhibit inflammatory processes, prevent an increase in the level of chemical markers of stress, have radioprotective properties, and the ability to detoxify the body from heavy metals (Soldatchenko S.S., Kashchenko G.F., Golovkin V.A., Gladyshev V.V. . Complete book on aromatherapy. Prevention and treatment of diseases with essential oils. 5th edition, expanded and revised. Simferopol: Tavrida, 2011).

Coriander hydrolate (Coriandrum sativum) contains: linalool - 60-80%, geraniol (3-5%), cymene, geranyl acetate - up to 5%, borneol - 1-4%, their acetic acid esters and aldehydes: n-decyl , decylene and isodecylene - 0.2-2.5%; terpenes, pinenes, phellandrenes. Coriander essential oil is considered a natural bactericidal agent; the ingredient linalool has an antibacterial and antifungal effect (Z.M. Nersesyan, A.Yu. Parkhomenko and others. Chemical composition and pharmacological activity of the herb extract of coriander sativum. News of higher educational institutions. North Caucasus region. Series: Natural Sciences. 2006:49-51). The disinfectant, detoxifying, antiseptic, antifungal and antioxidant properties of coriander are suitable for eliminating skin diseases such as eczema, dryness and fungal infections (Yunsung Hwang, Do Kyung Lee, Shin Hee Park, etc. Coriander leaf extract exhibits antioxidant activity and protects against photoaging of the skin induced by UV-B by regulating the expression of type I procollagen and MMP-1 (J Med Food, 09/01/2014).

The properties of sage essential oil (Salvia sclarea) are determined by the high content of esters - mainly linalyl acetate, linalool and the diterpene alcohol sclareol. Sclareol has anti-inflammatory activity. The mechanism of action of sclareol is associated with inhibition of the production of nitric oxide (suppression of inducible NO synthase of active macrophages), and cyclooxygenase-2 (COX-2), which leads to a decrease in the concentration of pro-inflammatory cytokines at the site of inflammation. Clary sage hydrolate is capable of providing antibacterial (a wide spectrum of antibacterial activity, including gram-positive St. аureus, gram-negative E. coli), fungicidal (antifungal, including Candida albicans), antiseptic, pronounced antispasmodic and anti-inflammatory effects (Zhilyakova E.T., Novikov O.O., Naumenko E.N., etc. Study of low molecular weight biologically active compounds of plant origin as promising agents for the prevention and treatment of seborrhea.Kuban Scientific Medical Bulletin.2010:122(8)68-72. ).

Terpene alcohol linalool is contained not only in hydrolates of coriander Coriandrum sativum, but also in hydrolates of angustifolia lavender Lavandula angustifolia Mill, clary sage Salvia sclarea, and has antiviral, antifungal, antibacterial, antiseptic and decongestant effects (Pashtetsky V.S., Nevkrytaya N.V. "Use of essential oils in medicine, aromatherapy, veterinary medicine and plant growing (review). Tauride Bulletin of Agrarian Science. 201:13(1):16-38).

The developed composition of the antibacterial agent makes it possible to take into account all the features of the plant hydrolates used and the most effective percentages of their introduction into the product with specified properties that are ideal for the skin.

The composition of the proposed product is stable and does not require additional introduction of preservatives and stabilizers. After treating the skin, the product remains on it in a certain amount sufficient to exhibit a bactericidal effect against gram-negative and gram-positive bacteria.

When creating the product, the concentrations of the active components, their set and combinations were taken into account; the maximum positive effects are observed when using the most complete extraction of active molecules from plant materials and when using low concentrations due to the fact that the responses require less energy expenditure.

The quantitative and qualitative content of components in the presented product is optimal and was selected on the basis of numerous experiments. Studies have shown that this composition of the product has a high speed of action.

The composition uses four hydrolates of medicinal plants that complement each other - mountain savory, coriander, clary sage, angustifolia lavender, which have an antibacterial and anti-inflammatory effect, have a beneficial effect on the skin with long-term use, and soothe the skin. The product is well absorbed and has a long shelf life. Biologically active substances of plant origin additionally moisturize and vitaminize the skin of the hands.

As a result of the experimental selection of the components of the proposed product, their synergistic effect is maximally manifested in the specified quantitative ratios, which provides a higher protective and preventive effect than known analogues and the prototype.

When components below the specified concentrations are introduced into the product, it does not provide sufficient antibacterial, protective and prophylactic effects, and when the specified concentrations of components are exceeded, no significant increase in these effects is observed.

The claimed invention is illustrated by the following graphic materials.

In FIG. Figure 1 shows a photo of the number of colonies (266 CFU/ml) on the palmar surface of the hand before treatment with an antibacterial agent.

In FIG. Figure 2 shows a photo of the number of colonies (168 CFU/ml) on the dorsum of the hand before treatment with an antibacterial agent.

In FIG. Figure 3 shows a photo of the number of colonies (1 CFU/ml) on the palm and dorsum of the hand 30 minutes after treatment with an antibacterial agent.

The inventive antibacterial agent for treating the skin of hands is prepared as follows.

In the production of the drug, technological equipment is used in the form of a reactor with a stirrer.

They use ready-made hydralates of mountain savory, coriander, Crimean angustifolia lavender and clary sage (Manufacturer - Federal State Budgetary Institution of Science "Research Institute of Agriculture of Crimea").

The amount of hydrolates of mountain savory and coriander, hydrolates of Crimean angustifolia lavender and clary sage, pre-measured according to the recipe of the claimed composition, is carefully poured into the container. Next, they are constantly mixed in a reactor with a stirrer at a speed of 50-60 rpm for 25-30 minutes. At the same time, a temperature regime of no higher than 20°C and normal pressure are maintained, which makes it possible to improve the dissolution of the ingredients and obtain a stable composition. Stirring is carried out until a homogeneous transparent solution is achieved. Then check the pH (5.0 to 6.0) of the finished product at room temperature.

At temperatures above 20° components of the hydrolates used may potentially be destroyed.

The finished product is fed to a dosing and filling apparatus, packaged in pre-prepared containers and sealed.

The composition of the proposed product was selected experimentally so that the mixture ultimately had the desired result, namely a result that allows for disinfection of hands and at the same time has additional effects - anti-inflammatory, softening and has a pleasant smell.

The antibacterial agent has high bactericidal activity, is stable and resistant to oxidation during storage, has an aqueous consistency and a pleasant odor, and can be used in spray form.

The product is produced using a single technology, using different concentrations of active components within the stated limits.

Examples of compositions made using the above-described technology for producing an antibacterial agent are given below.

Example 1.

Antibacterial agent 1 for hand treatment, wt.%:

Mountain savory hydrosol 30.0 Coriander hydrolate 25.0 Clary sage hydrolate 25.0 Hydrolate of Crimean lavender angustifolia the rest up to 100%

The product has a pronounced fungicidal and antimicrobial effect, as well as an anti-inflammatory effect.

Example 2.

Antibacterial agent 2 for hand treatment, wt.%:

Mountain savory hydrosol 25.0 Coriander hydrolate 23.0 Clary sage hydrolate 22.0 Hydrolate of Crimean lavender angustifolia the rest up to 100%

The product has a pronounced fungicidal, antimicrobial and anti-inflammatory effect.

Clinical trials were conducted to determine the antimicrobial properties of the components of the developed product.

The objects of research were reference strains S.aureus ATCC25923, E.coli ATCC 25922, C.albicans CCM 885, as well as clinical isolates of these microorganisms isolated from the pharynx of patients with COVID-19, from patients with pneumonia and from various biotopes of patients at a children's clinical hospital related to various species of the genus Candida and characterized by resistance to the antiseptic miramistin - a total of 19 cultures.

Studies of the effect of hydrolates were carried out in accordance with MUK 4.2.1890-04 (Determination of the antimicrobial activity of antibiotics and chemotherapy drugs) by the method of two-fold dilutions in a nutrient medium.

A suspension of daily crops with a density of 4.0 units was used in the experiments. McFarland turbidity. The effect of hydrosols of mountain savory, oregano, clary sage, coriander and lavender on fungal growth was determined in 96-well plates by changing the optical density of suspension cultures at a wavelength of 540 nm using a Multiscan device. The test samples contained double dilutions of hydrolates in a w/w medium 1: 1, 1:2, 1:4, 1:8; no hydrolates were added to the control samples. Culture densities were measured immediately after the introduction of microorganisms and at the end of the exponential growth phase. The ratio of biomass growth in experimental and control samples was expressed as a percentage. Results. Savory hydrolate 1:1 almost completely (95% or more) suppressed the growth of all tested crops, including isolates resistant to miramistin. Oregano hydrolate 1:1 inhibited fungal growth by 80-97%, depending on the isolate. Clary sage hydrolate 1:1 was less effective: growth inhibition in general ranged from 10 to 70%; in isolates from COVID-19 patients, this value was only 20-30%. A 1:1 dilution of coriander hydrolate reduced growth by 30-70%, depending on the crop tested. Lavender hydrolate 1:1 showed the weakest effect: growth inhibition was 10-50%, depending on the isolate. Hydrolates of savory and oregano demonstrated antibacterial and antifungal effects in a 1:2 dilution. A direct dose-effect relationship was observed for all hydrolates. Dilutions of hydrolates 1:8 had virtually no effect on the growth of bacteria and fungi. Conclusion. Hydrolates of mountain savory and oregano in dilutions of 1:1 and 1:2 had pronounced antifungal properties against all studied cultures of bacteria and fungi, including non-albicans species. The inhibitory effect of 50% (1:1) solutions of sage, coriander and lavender hydrolates depended on the culture tested.

Tests on volunteers of the proposed antibacterial agent showed that the product, which has an aqueous consistency, is easily applied to the skin of the hands and is well absorbed, without leaving a greasy sheen, and does not cause discomfort, has a pleasant smell, and does not have an irritating or allergic effect, does not cause has a negative effect on the morphology of the skin and, with prolonged application, has a positive effect on the skin of the hands.

The product was tested on 35 volunteers who provided voluntary informed consent for testing.

After an allergy test, the palmar and dorsal surfaces of the right and left hands were washed separately using sterile cotton swabs placed in an isotonic sodium chloride solution. To calculate the total microbial number, 1 ml of a swab sample was added to a Petri dish, mixed with warm meat-peptone agar (MPA), after the agar solidified, the dishes were incubated in a thermostat at 37°C for 24-48 hours. The number of grown colonies was counted visually and using the BIOMIC V3 Laboratory Plate Reader. The antibacterial composition was applied to the dorsum of the hand and incubated for 30 minutes. After 5, 10, 15 minutes and at the end of incubation (30 minutes), washes were made, followed by counting of colonies of microorganisms on agar after 24-48 hours of growth at 37°C.

The number of grown colonies before treatment with the drug averaged 250 CFU/ml on the palmar surface of the hand (Fig. 1).

The number of grown colonies before treatment with the drug averaged 116-168 CFU/ml on the dorsum of the hand (Fig. 2).

The antibacterial agent completely suppressed the growth of microorganisms within 15 minutes; after 30 minutes, only 1 colony -1 CFU/ml grew on the agar both on the palmar surface of the hand and on the back (Fig. 3).

Thus, an antibacterial hand skin treatment based on a mixture of medicinal plant hydrolates had a pronounced antimicrobial effect within 30 minutes. At the same time, 95% of those tested noted the pleasant smell and consistency of the product, as well as the moisturizing effect on the skin of their hands.

The proposed antibacterial product has a pleasant floral aroma and can be used at any time and anywhere without the use of water, soap, or wipes, and is capable of destroying 99.99% of the most common bacteria, fungi and viruses.

Also, this product does not leave a greasy film on the hands, contains substances that soften and nourish the skin of the hands, and is shelf stable. Use an antibacterial agent in the form of a spray by applying a small amount of liquid to the palms of your hands, followed by rubbing until dry. There is no need to rinse with water or wipe your hands with a napkin.

The bottle in the form of a spray has a convenient shape, does not take up much space, is compact, and allows you to quickly and reliably disinfect hands with the claimed product.

Studies have shown that using an antibacterial agent takes 15-20 seconds, but is much more effective than 1-2 minutes of thorough hand washing with soap and water, and it can be used as a protective treatment and prophylactic agent for treating both healthy and affected areas skin for the purpose of treatment and prevention of various fungal, pustular skin lesions. The product does not disturb the pH balance, stays on the skin for a long time, thereby protecting it from harmful factors, and does not have an irritating, drying or allergic effect.

The proposed qualitative and quantitative composition of the proposed product is necessary and sufficient to achieve the specified result. The components of the product in the specified ratio have the greatest activating antibacterial effect on the skin of the hands. In the proposed cosmetic product, in comparison with known analogues and the prototype, the loss of biologically active substances is minimized due to the possibility of preparation at room temperature and simplified technology.

Claims (2)

Antibacterial hand treatment, including phytoextracts of medicinal plants, characterized in that as phytoextracts it contains mountain savory hydrolate, coriander hydrolate, clary sage hydrolate and Crimean angustifolia lavender hydrolate in the following ratio of components, wt. %: Mountain savory hydrosol 25.0-30.0 Coriander hydrolate 23.0-25.0 Clary sage hydrolate 22.0-25.0 Hydrolate of Crimean lavender angustifolia The rest is up to 100%