RU2517031C1 - Biocidal composition for soaking napkins - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: biocidal composition for soaking napkins contains benzethonium chloride, sodium hydroxymethylglycinate, glycerine, decyl polyglucose, niacinamide, liposentol hydro, phenylmethanol and distilled water. Components are used in a defined ratio given in wt %.

EFFECT: invention improves biocidal properties of the napkin soaking composition.

3 tbl, 1 ex

Description

The invention relates to the field of hygiene and sanitation and can be used in medicine for the impregnation of napkins with their further use for treating hands and other areas of the skin in everyday life, in transport, in medical and child care institutions.

Recently, there has been a trend associated with the emergence of new infectious diseases.

In order to quickly, efficiently and safely localize epidemiological situations for humans and the environment, and to reduce the risk of the spread of infectious diseases, it is necessary to create antibacterial means of human protection.

For this purpose, moisturizing wipes with a biocidal effect, which create the effect of protecting the skin from external pathogens, can help the body.

Known antimicrobial composition for the impregnation of napkins, including an extract of a plant of the family buttercups, a surfactant and a tea tree essential oil.

As surfactants, alkyl ether sulfates or sulfonates can be used (US Pat. US No. 6951833, CL NCI 510/130, MKI - A01N 65/00, 2005).

This composition is effective against microorganisms of anthrax, pneumococci, streptococci and various fungi.

However, this composition may cause allergic reactions in certain consumers. In addition, the spectrum of microorganisms suppressed by this composition is limited.

Closest to the proposed invention is a disinfectant composition for the impregnation of wipes with the following components, wt.%:

- Clathrate carbamide didecyldimethylammonium bromide 0.15-0.30 - Ethanol 70.0-80.0 - Glycerin 0.05-0.50 - Perfumery fragrance FLORIAN 1206 firms N. Reynaud & Fils, France 0.01-0.05 - Nutritional Supplement - AMO-97 Bonifier firms N. Reynaud & Fils, France 0.001-0.006 - Distilled water rest

(Pat. RF No. 2132698, class A61L 2/16, 1999).

The known composition is used to impregnate wipes used to disinfect skin.

The disadvantages of the known composition include insufficient antimicrobial activity. In addition, the evaporation of the alcohol component included in the composition, worsen the consumer properties of wipes.

The technical problem solved by the invention is to increase the biocidal characteristics of the composition, as well as improve consumer properties, reduce the negative impact on the skin (dryness, cracks) with its frequent use and reduce allergenic characteristics.

To achieve the task in a biocidal composition for the impregnation of napkins, including a quaternary ammonium compound, glycerin and distilled water, benzethonium chloride is used as a quaternary ammonium compound and sodium hydroxymethyl glycinate, decyl polyglucose, niacinamide, liposentol hydro and the following components are added, with the following ratios: .%:

- benzethonium chloride 0.1-0.5 - sodium hydroxymethyl glycinate 0.1-0.3 - glycerin 0.1-0.2 - decyl polyglucose 0.1-0.2 - niacinamide 0.05-0.1 - liposenthol hydro 0.1-0.3 - phenylmethanol 10.0-12.0 - distilled water rest

The invention is illustrated as follows.

Benzetonium chloride is introduced into the composition as a biocidal component. Benzetonium chloride belongs to the group of quaternary ammonium compounds. The chemical formula C27H42O2NCI, molecular weight 448, is a colorless crystalline substance, odorless. Benzetonium chloride exhibits high biocidal efficacy on a wide range of bacteria and exhibits a powerful antibacterial effect when used in low concentrations, has low toxicity and practically does not cause allergic reactions.

Benzetonium chloride of the trademark "Lonzagard" manufactured by the company "Lonza" LTD, Switzerland is used.

Sodium hydroxymethyl glycinate is introduced as a bactericidal agent. It is a clear liquid.

Chemical formula HO-CH ₂ -NH-CH ₂ -COONa, gross formula

C ₃ H6O ₂ NNa, molecular weight 111. Sodium hydroxymethylglycinate has a wide spectrum of antibacterial action, and is also active against pathogenic fungi and yeast.

Use sodium hydroxymethyl glycinate trademark SUTTOCIDE, company ISP (CllIA).

Glycerin has a moisturizing effect on the skin. When introduced into the composition, glycerin gives it emollient and structural properties.

Glycerin (TU-6259-25) is a clear liquid. Promotes penetration of active substances into the skin and activates metabolic processes.

Decyl polyglucose is introduced into the composition as a wetting component to improve technological properties.

Decyl polyglucose - decyl glucoside is a non-ionic surfactant.

It is a surfactant derived from corn starch, wheat grain, and coconut fatty acids. Among surfactants, this substance is the safest with good dermatological compatibility, because is a completely natural product. It is a viscous yellowish aqueous solution.

Use decyl polyglucose of the trademark PLANTACARE 2000, company Cognis GmbH (Germany).

Niacinamide is introduced into the composition to improve its consumer properties.

Niacinamide is a nicotinic acid amide. It is an odorless white crystalline powder, soluble in water, alcohol, glycerin. Nicotinamide enhances the barrier function of the skin, has a stimulating effect on collagen synthesis, increases the content of skin lipids. It is a biologically active form of vitamin B3. A lack of this vitamin in the body leads to pellagra disease. Niacinamide slows down aging, strengthens the barrier function of the skin, reduces hyperpigmentation, irritation and red spots on the skin, eliminates the painful and yellowish color of the skin.

Niacinamide affects some strains of fungi that cause skin infections, protects cells from immunosuppression caused by UV radiation.

Niacinamide is used by the trademark Niacinamide of Kyowa Hakko Europe GmbH, Germany.

Liposentol hydro is a vitamin complex containing d-panthenol, ethyl panthenol, nicotinic acid, ascorbic acid, hyaluronic acid, salicylic and citric acid (TU RB 10154 1059.007-2000).

It is used as part of a product for intensive skin nutrition.

In addition, this component has a bactericidal effect and, in aggregate, enhances the bactericidal effect of the main disinfecting components of the composition - benzetonium chloride and sodium hydroxymethyl glycinate.

The combined interaction of benzethonium chloride, sodium hydroxymethyl glycinate and liposentol hydro has a stronger bactericidal effect on pathogenic microorganisms than each of these components individually.

The synergistic interaction of components between

by myself.

Phenylmethanol (GOST 8751-72) is used in the proposed tool as an antimicrobial component.

It is a clear, colorless liquid with a pleasant odor. It is soluble in water, alcohol, fatty and essential oils. The chemical formula is C ₆ H ₅ CH ₂ OH, molecular weight 108.

In selected concentrations, it is effective against gram-positive bacteria, and also has an effect against mold and yeast.

Phenylmethanol ensures the stability of the components of the composition, and also enhances the antiseptic properties of the Quaternary ammonium compounds - benzetonium chloride, which helps to increase the biocidal properties of the proposed composition.

Water is used as the basis of the composition according to GOST 609-84 (distilled) or purified.

The proposed composition is prepared as follows.

Benzetonium chloride, sodium hydroxymethylglycinate and phenylmethanol are dissolved in a container with water with thorough stirring. Glycerin, decyl polyglucose, niacinamide, liposenthol hydro and water are added to the resulting solution successively to a prescription amount. The mixture was thoroughly stirred at room temperature for 30 minutes. The resulting composition after checking the external indicators (compliance with specifications) is pumped into polymer containers.

Example No. 1 specific implementation

In a container containing 50 wt.% Water, benzetonium chloride 0.3 wt.%, Sodium hydroxymethyl glycinate 0.1 wt.% And phenylmethanol 10 wt.% Are dissolved with stirring.

Glycerol 0.1 wt.%, Decyl polyglucose 0.1 wt.%, Niacinamide 0.1 wt.%, Liposentol hydro 0.2 wt.% Are successively added to the resulting solution. Water in an amount of 39.2 wt.% Is added with stirring last.

The resulting mixture is thoroughly mixed at room temperature for 30 minutes, after which it is pumped into polymer containers.

The percentage of components of the biocidal composition is optimal and is identified on the basis of numerous experiments.

Other examples of specific performance, as well as proof of the correct choice of the content of the components within the stated limits are summarized in table No. 1.

The proposed composition is assigned to class IV of low-hazard substances. If necessary, fragrance can be added to the biocidal composition.

The antibacterial activity of biocidal compositions for the impregnation of napkins according to examples No. 1-5, as well as the composition of the prototype (example No. 1) was tested according to the method of “agar plates” in accordance with the Methodological guidelines for laboratory assessment of antimicrobial activity of textile materials containing antimicrobial agents approved The Ministry of Health November 18, 1983 in terms of indicator - growth retardation zone for Staphylococcus epidermidis (St. Epid. - epidermal staphylococcus) gram-positive cocci, belongs to the genus Staphylococcus. Despite the fact that St. Epidermidis is a non-pathogenic agent in some people with a weak immune system. Epid. may cause infections. St. Epidermidis can cause both nosocomial and community-acquired infections. St. Growth Retardation Area Data Epid. are given in table 2.

In addition, the antibacterial activity of napkin impregnation biocides was tested according to the indicator - growth retardation zone according to S. Aureus (gram-positive microorganisms), E. coli, Ps.aeruginosa (gram-negative microorganisms) upon diffusion of the test substance into a dense nutrient medium, as well as determination the minimum time required to completely suppress the growth and development of test microorganisms (Fedorov Yu. A., Koren V.N. “Microbiological methods for the study of dental hygiene products” in the book “Fundamentals of oral hygiene.” L., “Medicine”, 1973, p. 3-215).

For this, 20 ml of solid nutrient medium was poured into a Petri dish. A 24-hour microbial suspension of test microorganisms with a concentration of ⁵ × ^{10 5} CFU / ml was prepared and 1 ml of suspension was applied to the surface of the nutrient medium. They were dried for 30 minutes, and then wells with a diameter of 6 mm were made in a nutrient medium, into which samples of the developed biocidal composition were applied for the impregnation of napkins. The number of holes should not exceed 6 per Petri dish. Petri dishes were kept at room temperature (20 ° C) for 30 min, then they were placed in a thermostat for 24-48 hours at 37 ° C, and after this period, zones of inhibition of microbial growth, including the diameter of the wells, were taken into account. By the size of the zones of growth inhibition of test microorganisms, the antimicrobial effect of the test substance was judged, although it should be borne in mind that the diffusion rate of polymer disinfectants is lower than the diffusion rate of low polymer disinfectants.

To obtain reliable results, all experiments were performed in triplicate, the testing time was 48 hours. The experimental data were processed statistically.

Data on the zone of growth retardation for S. Aureus, E. coli, Ps.aeruginosa are shown in table 2.

An analysis of the data presented in Table 2 shows that the tested biocidal napkin impregnation compositions have a high level of antibacterial activity, the most sensitive to the claimed biocidal compositions were gram-positive microorganisms (S. aureus), growth retardation zones for which ranged from 37 to 41 mm. High rates were also observed in suppressing the growth of E. coli, Ps.aeruginosa and St. Epid.

The high biocidal effect observed as a result of the study is ensured by the combined interaction of the disinfecting components of the composition - benzethonium chloride and sodium hydroxymethyl glycinate, phenylmethanol with the vitamin complex - liposenthol hydro, enhancing their combined biocidal effect, as well as the presence of a wetting agent - decyl polyglucose and glycerol, which provides better penetration niacinamide and the vitamin complex liposentol hydro, which contributes to the therapeutic effect to minor skin lesions cover.

The composition of the prototype had antibacterial properties, but slightly lower than the declared biocidal compositions for the impregnation of wipes.

To determine the minimum time required to completely suppress the growth and development of test microorganisms, a suspension of test microorganisms in the amount of 10 ⁵ CFU / ml was added to the test substance; for this, 1 ml of the test substance was poured into test tubes. The tubes were shaken and, at intervals of 2-5-10 and 30 minutes, were sown on solid nutrient media. Petri dishes were placed for 24-48 hours in a thermostat at 37 ° C. After this period, the number of colonies grown was counted and the bactericidal activity of the test substance was judged by this indicator.

The results of the studies are presented in table.3.

From the data presented in Table 3, it follows that the antibacterial effect of the developed biocidal compositions develops very quickly. After 2 minutes of contact, in no case was live microflora detected after contamination of the nutrient medium with Escherichia coli.

The biocidal composition for napkin impregnation was tested by volunteers in an accredited specialized clinic, as a result of which the biocide composition has a high effect on the skin, it is noted that the biocidal composition does not have an irritating and allergenic effect.

Napkins impregnated with the proposed biocidal composition for impregnating napkins can be used for hygienic treatment of hands, sanitary treatment of skin, for the prevention of pressure sores, for the care of bedridden patients.

Table 1 No. Benzeto Hydroxy Glice Decile Niacin Liposen Phenyl Water Technical result p / p niya methylgly rin polyglyu mid tol meta chloride sodium cinnamon goat hydro zero Content in wt.% one 0.3 0.1 0.1 0.1 0.1 0.2 10.0 rest The composition has high biocidal 2 0.2 0.1 0.1 0.1 0.08 0.2 10.0 rest characteristics, does not dry skin, does not 3 0.1 0.3 0.15 0.2 0.05 0.1 12.0 rest  causes allergies, has a therapeutic effect on four 0.5 0.3 0.2 0.1 0.1 0.1 10.0 rest against damaged skin, does not contaminate 5 0.5 0.2 0.2 0.15 0.08 0.3 11.0 rest environment. 6 0.05 * 0.3 0.1 0.1 0.1 0.1 10.0 rest Decrease in biocidal action 7 0.55 * 0.3 0.1 0.1 0.1 OD 10.0 rest Consumer Impairment 8 0.3 0.05 * 0.1 0.1 0.1 0.1 10.0 rest Decrease in biocidal action 9 0.3 0.35 * 0.1 0.1 0.1 od 10.0 rest Consumer Impairment 10 0.3 0.3 0.05 * 0.2 0.1 0.1 10.0 rest Decrease in technological characteristics eleven 0.3 0.3 0.25 * 0.2 0.1 0.1 10.0 rest Increasing the stickiness of the composition 12 0.3 0.3 0.1 0.05 * 0.1 0.1 10.0 rest Reduced wettability of wipes 13 0.3 0.3 0.1 0.25 * 0.1 od 10.0 rest Technological degradation fourteen 0.3 0.3 0.1 0.1 0.01 * 0.1 10.0 rest Reduced consumer properties fifteen 0.3 0.3 0.1 0.1 0.11 * 0.1 10.0 rest Unreasonably high component consumption 16 0.3 0.3 0.1 0.1 0.1 0.05 * 10.0 rest Decrease in biocidal effect 17 0.3 0.3 0.1 0.1 0.1 0.35 * 10.0 rest Unreasonably high component consumption eighteen 0.3 0.3 0.1 0.1 0.1 0.1 9.0 * rest Decrease in biocidal characteristics 19 0.3 0.3 0.1 0.1 0.1 0.1 12.5 * ost Increased allergenicity of the composition

Note: * - the content of the component is beyond the stated limits.

table 2 Samples of the biocidal composition for the impregnation of napkins Microorganism growth inhibition zones, mm S. aureus E. coli Ps.aeruginosa St. Epid. Example 1 38 26 27 23 Example 2 37 25 26 22 Example 3 39 27 27 23 Example 4 41 29th 29th 25 Example 5 40 28 28 24 The composition of the prototype (Example No. 1) 36 24 25 21

Table 3 Samples of the biocidal composition for the impregnation of napkins Seeding * after a period of time (min) 2 5 10 thirty Example 1 not upd. not upd. not upd. not upd. Example 2 not upd. not upd. not upd. not upd. Example 3 not upd. not upd. not upd. not upd. Example 4 not upd. not upd. not upd. not upd. Example 5 not upd. not upd. not upd. not upd. The composition of the prototype (Example No. 1) not upd. not upd. not upd. not upd.

* bacteria of the species E. coli, 10 ⁵ CFU / cm ³

Claims (1)

A biocidal composition for impregnating napkins, including a quaternary ammonium compound, glycerin and distilled water, characterized in that benzethonium chloride is used as a quaternary ammonium compound and additionally contains sodium hydroxymethyl glycinate, decyl polyglucose, niacinamide, liposentol hydro and phenylmethanol components in the following ratio. %:
- benzethonium chloride 0.1-0.5 - sodium hydroxymethyl glycinate 0.1-0.3 - glycerin 0.1-0.2 - decyl polyglucose 0.1-0.2 - niacinamide 0.05-0.1 - liposenthol hydro 0.1-0.3 - phenylmethanol 10.0-12.0 - distilled water rest