JPH1156984A - Quick disinfecting method for combinedly polluted object with ozone water - Google Patents

Abstract

PROBLEM TO BE SOLVED: To attain quick and complete disinfection with a small quantity of ozone water having the concentration as low as possible by pre-washing a disinfection object with a surface active agent, removing other polluted materials coexisting with microbes, and applying disinfection with the ozone water in this disinfecting method for the combinedly polluted object with the ozone water. SOLUTION: A disinfection object combinedly polluted by the coexistence of microbes with materials such as an oxidized material and oily and filmy solids is disinfected by this ozone water disinfecting method of the combinedly polluted object with ozone water. The disinfection object is pre-washed by a surface active agent, and the other polluted materials coexisting with the microbes are removed. The disinfection object is disinfected with the ozone water, the sterilizing/disinfecting capability of the ozone water is effectively applied to the microbes, and a quick and complete disinfection result is obtained with a small quantity of the ozone water having the concentration as low as possible.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a simple, rapid, safe and thorough disinfection and disinfection of body skin or objects which are complexly contaminated with a substance which consumes a large amount of ozone, using ozone water. It relates to a basic disinfection method for realizing complete and efficient.

[0002]

2. Description of the Related Art In recent years, the types and frequency of occurrence of infectious diseases have increased remarkably, and perfect disinfection techniques have been demanded by all layers of society. However, conventional commercial disinfectants and disinfecting methods using them have the following problems, and there is no simple, rapid, safe, complete and efficient disinfecting method.

[0003] Currently available disinfectants are not effective against all pathogenic microorganisms, and are often ineffective against newly emerging drug-resistant bacteria. In addition, high levels of use of disinfectants, rough hands, on which also frequent side effects such as dermatitis, is an essential wastewater treatment after use, not be able to ignore and child adverse effect on the activated sludge. In addition, there is a problem that it is difficult to cope with an emergency, for example, it takes a long time such as tens of minutes or several hours to complete the disinfection. For this reason, a method using ozone water having an excellent sterilizing power has been studied.
9738.

[Problems to be solved by the invention]

In the ozone water disinfection method of the present inventors,
It has already been confirmed that sterilization is completed in a short time in seconds with ozone water having a concentration of 4 ppm, which is more dilute than the invention described in the above-mentioned publication, and greatly exceeds the performance described in the invention in both the concentration and the contact time. . Here, the difference between the accuracy of the ozone water production technology and the disinfection method is deeply involved. The ozone water disinfection method of the present inventors eliminates the problems associated with the above-mentioned commercial disinfectants and methods of use without omission,
Since the requirements for practical use are satisfied, these contents have already been applied for a patent as 1993 Patent Application No. 39548. However, in the subsequent clinical trials, we encountered a case where a strong inhibition phenomenon on ozone water sterilization due to the following complex contamination was remarkably manifested, and establishment of countermeasures against the inhibition phenomenon has become an essential requirement.

That is, most of actual disinfection objects are complexly contaminated with various kinds of organic and inorganic substances in addition to bacteria, and these contaminants dissolve dissolved ozone in ozone water. The potential for consumption or decomposition is extremely high. For this reason, the excellent sterilization performance and disinfection effect of ozone water obtained in the in vitro experiment of ozone water disinfection cannot be reflected in clinical test results, and the opinion that "ozone water disinfection is practically ineffective" has become established. I am doing it. On the other hand, the emergence and spread of various infectious diseases is a major problem that will shake the foundations of modern society, and disinfectants that can reliably cope with all these infectious diseases are ultimately difficult to find other than ozone water. There are facts. On the other hand, ozone water has a strong bactericidal action, while dissolved ozone is extremely unstable, easily decomposes and returns to harmless oxygen, and has excellent future-oriented characteristics that it is extremely friendly to the environment. Therefore, it is important to elucidate the inhibitory factors of the excellent disinfection performance of ozone water, establish countermeasures corresponding to the actual situation of the inhibition phenomenon, and promote the spread of ozone water disinfection. The elucidation and overcoming of the obstruction to ozone water disinfection can be achieved by fully utilizing the excellent disinfection performance inherent in ozone water to generate great medical benefits and the characteristic of ozone water that has no fear of environmental pollution. One of the advantages not found in traditional commercial disinfectants is that it is a significant technological development in global environmental protection.

[0006]

Means for Solving the Problems In order to solve the above problems, a method for rapidly disinfecting a complex contaminated object with ozone water according to the present invention is characterized by employing the following means. (1) A method of disinfecting an ozone water disinfecting a complex contaminated object which disinfects an object to be disinfected that is complexly contaminated by the coexistence of microorganisms and oxidizable substances and / or substances such as oily, filmy and solid substances with ozone water. The object is pre-washed with a surfactant to remove other contaminants that coexist with microorganisms, and then the object to be disinfected is disinfected with ozone water so that the microorganisms can have the sterilization ability of ozone water. It is effective to make it possible to quickly and completely sterilize and disinfect with ozone water having a low concentration and a small dose. (2) N which consumes less dissolved ozone as a surfactant
-An acyl-N-alkyltaurine alkali salt or an agent having the same effect. (3) The N-acyl-N-alkyltaurine alkali salt of the surfactant or the same effective agent is preferably used at a concentration of 1%.
Use 3-10 mL as an aqueous solution of ２０20%, most preferably around 5% concentration. (4) The concentration of ozone water for hand washing disinfection is preferably 2 to 20 ppm, most preferably 3 to 5 ppm,
The amount used is preferably 10 mL to 2 L, most preferably 5 mL.
It is from 00 mL to 1 L. (5) The concentration of ozone water for disinfecting affected parts or wounds is preferably 10 to 25 ppm, most preferably 17 to 25 ppm.
23 ppm, and the amount used is preferably 10 mL to 5 mL.
L, most preferably from 500 mL to 1 L. (6) The concentration of ozone water for disinfecting equipment is preferably 25 to 70 ppm, most preferably 36 to 44 ppm.
And the amount used is preferably 1 L to 20 L, most preferably 1 L to 5 L. (7) Ozone water for disinfection of various bacteria, virus rickettsiae, amoeba and the like can completely kill them all at a concentration of 4 ppm. (8) Ozone water for disinfection of amoeba which formed cyst
Use a concentration of 40 ppm or more. (9) The waste liquid used for disinfection is temporarily stored, and is discharged after confirming the completion of sterilization and disinfection by the presence of excess dissolved ozone. (10) Pre-cleaning with a surfactant and disinfection with ozone water are performed using a semi-automatic or fully automatic apparatus. The method for quickly disinfecting a complex contaminated object with ozone water according to claim 1. (11) The disinfection method using ozone water is selected from a shower down method for hand washing disinfection and a dipping / washing method, a spray method, or a wiping method for disinfection of articles so that the best disinfection effect is always obtained. .

[0008]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be described below based on experimental examples and the like.

[0009] Frequently, strict sterilization and disinfection are required for medical personnel in hospitals and clinics, especially for medical workers working in operating rooms and intensive care facilities, where patients are excreted. There is a considerable risk of contamination by contaminants containing oxidizable substances such as substances, secretions or blood. These wastes contain ozone consuming substances and have a great risk of inhibiting the disinfecting effect of ozone water. Although the soiling of the fingers accompanying the disposal of these wastes should have been washed away by encouraging hand washing, it is not impossible that some soiling remains. The slight contamination may have a fatal adverse effect on the disinfection effect of ozone water, but no detailed quantitative research data is available on the effect.

[0010] Thus, as an example of these contaminants, the effect of human serum on ozone water disinfection effect was examined in vitro in Experiment 1.
Investigation by ro experiment showed that the results shown in Table 1 were obtained, and it was confirmed that a very small amount of human serum exhibited an extremely strong disinfection inhibitory effect.

[0011]

[Table 1]

In this experiment, 4 ppm ozone water, which was determined as a reference concentration of ozone water for hand washing and disinfection, was used. There are four bacterial species, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus, which are the most representative of hospital-acquired infections. The outline of the experiment is described in Experiment 1.

[0012]

[Experiment 1] Disinfection inhibitory effect of human serum 0.050 mL of human serum was placed in a small test tube, 0.5 mL of bacterial suspension was added thereto, followed by stirring, and then 4.5 mL of 4 ppm ozone water was added to start time measurement. And continue stirring.
After 0 second, 1 / 40N sodium thiosulfate TS 0.050
Add mL and stir to stop the germicidal reaction. 1 mL of the treated solution was collected, transferred to an agar medium, cultured at 37 ° C. for 24 hours, and the number of remaining bacteria was counted. The above experiment without human serum was performed in the same manner, and the effects based on the presence or absence of human serum are compared in Table 1. The bacterial species tested are the four species described above, which are representative of opportunistic infections in hospitals. The concentration of the bacterial suspension was determined by sampling using a standard dilution method during the experiment, and measuring the number of bacteria by the same method simultaneously with the experiment. Table 1 shows the results of the above experiments. ∞ in the table indicates that the number of remaining bacteria was too large to be measured.
The results of this experiment show that 4 ppm ozone water completely kills hundreds of millions of bacteria in a contact time of 30 seconds, and that the bactericidal effect is complete assuming that this bacterial suspension contains 10% serum. It clearly shows that it should disappear.

In this experiment, the treatment time with ozone water was 3
Although it is 0 seconds, an experiment in which this is reduced to the unit of 1 second is impossible with the present experimental system using test tubes. The reason is that the test tube type experimental system requires at least several seconds or more for each operation step of adding and mixing the reagent. Therefore, an experimental system was examined and 1
A new method capable of conducting experiments in seconds is developed, and the results are described in Experiment 3.

On the other hand, in order to chemically analyze this inhibition phenomenon, the amount of ozone consumed by human serum was quantified by an oxidation-reduction method.
It was found that the mL was consumed very quickly. The outline of the experiment is shown in Experiment 2.

[0015]

[Experiment 2] Ozone water consumption of human serum Accurately measure 50 μL of human serum, add 50 mL of 4 ppm ozone water, mix with stirring, and immediately dilute 1 mL of dilute sulfuric acid.
And 1 mL of iodine potash solution, and add 0.5% starch solution
After adding mL and stirring, the test solution turns blue-violet due to the oxidizing action of dissolved ozone. This color test solution is titrated with a standardized 1 / 40N sodium thiosulfate ruler solution, and when the blue-violet color of the test solution disappears, the titration value is measured as the end point, and the measured value is defined as the amount of dissolved ozone in the test solution corresponding to 4 ppm ozone water. 6.8 mL was calculated. This calculated amount of ozone water corresponds to the remaining amount obtained by subtracting the amount consumed for serum oxidation from the initially added amount of 4 ppm ozone water of 50 mL. Therefore, 50 μL of human serum (=
0.050 mL) is 43.2 mL of 4 ppm ozone water
Is calculated. That is, human serum 1.0 mL
Consumes 864 mL of 4 ppm ozone water.

Considering the results of Experiment 1 from these results, 0.050 mL of the serum used in Experiment 1 was 4
It was concluded that it was a very natural result that 4.5 mL of ppm ozone water was completely consumed, and there was still a large excess in excess, and the complete inhibition phenomenon was measured. Therefore, if 1 L of 4 ppm ozone water is used for hand washing and disinfection, only a little less than 1.2 mL of trace serum adheres,
The dissolved ozone in the used ozone water is completely consumed without any residue, and the result is that the residual amount of dissolved ozone to be used for the original sterilization purpose is zero. This result can be further thought from a chemical point of view as follows. Serum, blood, concentrated juice,
Oxidizable (or reducing) such as secretions or excretions
Substances containing components strongly inhibit ozone water sterilization.

As a mechanism of sterilization by ozone water, the fact that cell walls and cell membranes constituting the outer wall of the cells are almost instantaneously oxidatively destroyed by contact with ozone water has been confirmed by microscopic observation. The fact that the effluent cell contents were also rapidly oxidized by excess ozone was chemically confirmed. In this oxidation reaction, it is clear from the in vitro experiment of Experiment 3 that one cell instantly consumes an estimated 40 attogram (one atogram is one in a billionth or one billionth of a gram more) of dissolved ozone. became. Here, it was necessary to develop a new experimental method that enabled tracking in seconds.

[0018]

[Experiment 3] In order to elucidate the sterilization mechanism using ozone water, it is necessary to find a time course. However, in the test tube method shown in Experiment 1, the time required for mixing and stirring the reagents is hindered, and a short processing time in seconds is required. Becomes practically impossible. Therefore, a micro assay plate (8 ×
12), and a method for minimizing a processing time error by simultaneously processing various kinds of bacterial species by using a multi-channel pipet was devised. As a result of this downsizing, the solution in the hole was instantaneously stirred and mixed by the injection of the test solution, and the problem of the time required for stirring in a conventional test tube scale could be solved.

25 μL of each bacterial solution is dispensed into a micro assay plate for experiment using a multi-channel pipette, and 225 μL of 4 ppm ozone water is injected with the multi-channel pipette, and time measurement is started. At a predetermined time, 5 μL each of a 1 / 40N sodium thiosulfate solution is injected as a stop solution to stop the bactericidal action. The whole amount of the reaction solution is taken out from each hole of the microassay plate, the attached liquid in the hole is washed out with a small amount of sterile physiological saline, combined with the reaction solution, and transferred to each petri dish. Heart infusion agar medium is added to each Petri dish, and the number of remaining bacteria is counted after culturing at 37 ° C. for 24 hours. Coagulase types 1,2,4 of Staphylococcus aureus MRSA with drug resistance
Compared to S. aureus MSSA having no drug resistance using 5 and 5 types, a total of 8 types of bacteria were used by adding Pseudomonas aeruginosa and Serratia as subjects. All of these bacteria are collected from the culture solution, added with an appropriate amount of sterile physiological saline, stirred, and centrifuged.This washing operation is repeated several times to thoroughly remove substances adhering to the bacteria. The test was suspended in saline and used for the test. As in Example 1, the concentration of this bacterial solution was sampled at the time of the ozone water sterilization experiment by applying the serial dilution method, and cultured and counted in the same manner as the test solution.

Table 2 shows the results of the sterilization experiments performed on these bacterial species by applying the microassay plate method described above. The numbers in the table represent the number of remaining bacteria.

[0021]

[Table 2]

Table 2 shows that the sterilization with ozone water showed that the microassay plate method was suitable as an experimental method and that it was almost completely completed in 5 seconds regardless of the bacterial species and the presence or absence of drug resistance. It is clearly shown.

Next, Table 3 shows test data obtained under the same conditions as in Table 2 by increasing the concentration of these bacteria.

[0024]

[Table 3]

The high-concentration bacterial suspension containing 1 trillion trillion bacteria in 1 mL used in the experiments in Table 3 was a viscous liquid in which the fluctuation of the bacterial groups could be visually observed, and the uniformity of the bacterial cell dispersion was high. And the reproducibility of the amount collected by the micropipette is not good.
Due to these circumstances, the data in Table 3 this time has a larger fluctuation range in the count value than the data in Table 2, and the time-dependent fluctuation in the number of remaining bacteria is random. In addition, in any bacterial species, before the injection of the reaction stop solution, zero ozone concentration in the test solution was qualitatively observed in the iodine potash starch paper,
The experiment in Table 3 can be concluded as a sterilization experiment performed under the condition that the absolute amount of dissolved ozone was slightly less than the required amount of sterilization. That is, the entire amount of dissolved ozone in the used ozone water is consumed in the sterilization reaction, and the residual ozone amount becomes zero, and only a part of the bacteria survives. Estimated from this fact, one bacterium is about 40 attograms of dissolved ozone.
Is consumed almost instantaneously, and the difference between the bacterial species is not remarkable.

As a result of such a rapid chemical reaction, if the absolute number of cells is too large and the amount of ozone water used is less than the approximate value consumed by the cells, the number corresponding to the dissolved ozone amount The cells are destroyed by ozone, the entire amount of the dissolved ozone is quickly consumed and exhausted, and the oxidizing power is completely lost,
The surviving cells survive. In other words, an essential condition for completely performing sterilization and disinfection with ozone water is that the absolute amount of dissolved ozone exceeds the required amount described above, and it is only necessary to specify one of the concentration and the amount of ozone water used. Insufficient.

By the way, 1 mL of 4 ppm ozone water completely destroys about 100 billion bacteria as the number of cells within a few seconds without leaving any of the above bacteria.
It turns out that it is equivalent to an extremely strong sterilizing power.
Compared to the bactericidal action of Experiment 3, Experiments 1 and 2
It is clear that the inhibitory effect of human serum shown in (1) is extremely strong.

This is a phenomenon which is almost neglected in a general disinfectant used at a high concentration close to the order of%, and is a phenomenon peculiar to ozone water at a low concentration of the order of ppm, which is nearly 1,000 times thinner than these. is there. This is deeply related to the nature of the ozone water disinfection mechanism, which is a rapid and sensitive oxidation reaction, and therefore a reaction consumption type that quantitatively consumes dissolved ozone along with the disinfection reaction as described above. .

Considering the essence of ozone water sterilization, the above-described inhibition phenomenon is not limited to human serum, but is applied to various biological substances such as blood, pus, exudate, cough, and saliva. It can be seen that this is a common inhibition phenomenon. Therefore, in ozone water disinfection, especially in hand washing and disinfection in medical institutions, it is indispensable to always carry out disinfection on the assumption that there is compound contamination by these inhibitors. This is a fundamental difference from the conventional disinfectant.

Measures to cope with the coexistence of a strong inhibitor include (1) a method of using a large amount of ozone water which surpasses the inhibitor, and (2) removal of contamination by the inhibitor by the action of a surfactant. Two strategies are possible.
(1) is uneconomical in that it requires the use of an extremely large amount of ozone water, and has a problem that it is unclear at which point in time the sterilization operation should be completed. On the other hand, in (2), the cleaning ability with the surfactant is excellent, and the operation is simple and the cost is low. In addition, the use of surfactants has the effect of reducing ozone consumption by removing oxidizable contaminants and oily contaminants from the surface of the object to be disinfected, and improving the water wettability of the surface of the object to be disinfected by using ozone water. The effect of increasing the probability of contact with water is also great, and the disinfection effect of ozone water can be significantly improved based on these two effects.

Therefore, most of the above-mentioned coexisting contaminants are removed by the use of a surfactant prior to ozone water disinfection and disinfection, and the original purpose of the disinfection and disinfection ability of the ozone water is most. We reach the conclusion that turning to sterilization is the most reasonable. Here, it is necessary to select a substance which consumes a small amount of ozone as the surfactant, and N-acyl-N-alkyltaurine alkali salts can be used for this purpose.

In this method, the object to be disinfected can be sterilized to a sterile state, but in the case of biohazard countermeasures, sterilization of the disinfecting waste liquid is also an essential requirement. When it is necessary to strictly observe such biosafety considerations, excess ozone water is injected into the disinfecting waste liquid, stored for several minutes or more, and waited for completion of the sterilizing action. It may be discharged after confirming the remaining ozone. However, if necessary, after the surfactant aqueous solution is applied to the entire surface of the object to be disinfected, ozone water may be applied to simultaneously remove and disinfect the coexisting contaminants.
In this case, in consideration of the amount of dissolved ozone consumed by the surfactant, it is necessary to increase the amount of ozone water or the amount of ozone water or both in the ozone water used.
Also in this case, it is essential to check the residual ozone in the disinfecting waste liquid.

When the complex contaminants are firmly attached to the surface of the object to be disinfected, the desorption of the contaminants is promoted by using the surfactant aqueous solution and the ultrasonic irradiation together to promote the decontamination. The sterilization can be completed by adding an excessive amount of high-concentration ozone water while keeping the container submerged. In the case of disinfecting the articles in this way, the concentration of ozone water is increased, and for example, high-concentration ozone water of 40 ppm or more is used, and the efficiency of disinfection work can be increased. In any of the above cases, treatment of surplus ozone is extremely simple and easy, and there is no need to provide a special water treatment facility. One liter of the hospital's total wastewater consumes dissolved ozone in about 20 L of ozone water with an ozone concentration of 4 pp very easily and in a short time simply by mixing these two solutions at room temperature. Is zero, so it is considered that there is no adverse effect on the environment at the destination.

Example 1 shows the results of an experiment for disinfecting a composite contaminated object treated by this method. From these results, it was proved that prevention of outflow and prevention of contamination of harmful microorganisms can be completely realized, that is, the application of the method of the present invention is effective from the viewpoint of biosafety. A combination of a surfactant and ultrasonic irradiation is effective for a fine place such as a tweezer tip or a syringe needle, or a place where a bloodstain adheres. The method of the present invention is fully applicable when biohazard measures are required.

In the case of hand washing and disinfection, the above-mentioned ozone water having an ozone concentration of 4 ppm is most preferably used from the viewpoint of safety to living bodies, and hand washing and disinfection are carefully continued from the beginning using a large amount of 4 pp ozone water. In addition, the wastewater from the hand washing is temporarily stored in a waste liquid container, and after the amount of ozone remaining in the waste liquid is confirmed and released, the microorganisms can be reliably prevented from diffusing into the environment.

Not a small number of bacteria were washed out and survived in the washing waste liquid using the surfactant, and the number of bacteria reached several hundred times the measured value before the start of hand washing. Can be This fact indicates that (1) bacteria attached to the skin are destroyed by coexisting contaminants, and bacteria buried and coated in toes, wrinkles and prints are particularly difficult to wash out, and gloves that are massaged and washed with physiological saline That the number of bacteria washed out in the juice solution is small, (2) the number of bacteria in the pre-wash waste liquid has increased significantly because the surfactant has peeled off the coating and the washing out of attached bacteria has been facilitated, 3) The action of the surfactant increases the hydrophilicity of the skin, significantly increasing the contact efficiency with ozone water, effectively exhibiting the disinfecting effect of ozone water, and drastically reducing the residual bacteria on the skin after disinfection; (4) This indicates that the surplus ozone in the hand-washed ozone water waste liquid exceeded the amount of dissolved ozone consumed by contaminants in the waste liquid, so that the residual bacteria count in the waste liquid was zero. This result clearly shows that the use of surfactants is extremely effective for bacteria with multiple contamination.

In the case of normal hand washing and disinfection which is most frequently used, the above two processes of pre-washing and ozone water disinfection are installed in an ozone water apparatus as software for automatically proceeding, and automatically controlled hand washing and disinfection. It can be used as an ozone water supply device. Example 3 shows the results of a panel test using a standard ozone water supply apparatus for hand washing and disinfection equipped with an automatic control system. Further, the data in Table 6 shows that superior results can be obtained even in a short time as compared with the conventional hand-washing disinfection using a commercially available disinfectant.

In this case, if the standard concentration of ozone water is most preferably 4 ppm and the amount of ozone water is 500 mL to 1 L, safety for the user can be sufficiently ensured, and thorough disinfection and disinfection with ozone water can be easily performed. It has the advantage that it can be realized quickly, safely, completely and efficiently, and that even if the wastewater from hand washing is discharged without treatment, the adverse effect on the environment due to dissolved ozone can be almost completely avoided. This ozone water concentration is 2 pp
It is possible to extend the range from m to 20 ppm, but in these cases, it is necessary to appropriately change the amount of ozone water used based on the nature of the ozone water sterilization.

The above-mentioned sterilization method using ozone water is applied to a simple disinfection method usually used, such as showering, dipping, washing, spraying, and wiping, so that complete disinfection can be achieved quickly and reliably. . For sterilization of equipment,
It is more effective to add a physical method such as ultrasonic irradiation. In addition, these disinfection methods can be incorporated into a disinfection device to automate and simplify the entire disinfection procedure. Conventionally, the reason why sterilization and disinfection with ozone water has not been put to practical use is due to insufficient disinfection effect by ignoring the effects of complex contamination, especially complex contamination by oily substances or oxidized substances. The method eliminates these difficulties and achieves a perfect disinfection effect.

An embodiment of the method for rapidly disinfecting complex contaminated objects with ozone water according to the present invention will be described below.

[0042]

EXAMPLE 1 Using a set of used forceps, a scalpel, tweezers, and a syringe with a needle after use, the disinfection method was examined as follows. These objects to be disinfected are put together in a glass container, and 500 mL of distilled water is added and stirred to collect 1 mL of the test solution (A), and 1 mL of a surfactant (5% aqueous solution of sodium lauroylmethyltaurine) is added thereto and stirred for 3 minutes. Then, 1 mL of the test solution (B) is collected, and the whole glass container is transferred to an ultrasonic cleaner, and irradiated with 40 kHz ultrasonic waves for 3 minutes to collect 1 mL of the test solution (C). 40 ppm to this
Then, 500 mL of ozone water is added, and the mixture is stirred for 1 minute. After confirming excess of ozone with iodine potato starch paper, 1 mL of test solution (D) is collected. The washing solution is drained, sterilized distilled water (500 mL) is added to the disinfected vessel, and ultrasonic waves are irradiated for 3 minutes. Then, 1 mL of the test solution (E) is collected to examine the disinfecting effect. Transfer each test solution to Heart Infusion Agar Medium,
After culturing at 37 ° C. for 24 hours, the number of bacteria was counted. Table 4 shows the results.

[0043]

[Table 4]

[0044]

Example 2 In hand washing and disinfection of persons engaged in highly contaminated work, natural contamination before washing (A) and the number of bacteria in the pre-wash waste liquid (B) with a surfactant, which were used for hand washing and disinfection. The number of bacteria in the waste liquid (C) into which 1 L of 4 ppm ozone water had been combined and the number of bacteria remaining (D) on fingers that had been hand-washed and disinfected with the ozone water were measured. In (A) and (B), adherent bacteria were washed into 50 mL sterile physiological saline by the glove juice method, and 1 mL thereof was used for cultivation.
Multiply by the amount of bacteria adhered by natural contamination. In (B), 1 mL of 1 L of the pre-wash waste liquid was used for the culture, the number of bacteria was multiplied by 1,000 to obtain the total number of bacteria in the pre-wash liquid, and (C) was treated in the same manner as in (B) and 2,000-fold. To determine the total number of bacteria. The culture was performed on a heart infusion agar medium at 37 ° C for 24 hours.
After incubation for a period of time, the number of remaining bacteria is counted. Table 5 shows the results.

[0045]

[Table 5]

Incidentally, the glove juice method is a careful hand washing method using sterile water, and it has been said that in the case of simple contamination, the total number of most bacteria adhering to a finger can be measured. However, when handling oily or sticky composite contamination, it is difficult to remove microorganisms mixed or encapsulated in the composite contamination by water washing alone. The fact that the number of bacteria in the washing waste liquid (B) using a surfactant is several hundred times greater than that in the glove juice method (A) is presumed to be due to the above-mentioned severe contamination, and the effect of the surfactant is clear. is there. Also, the remarkably large standard deviation from the average value of the glove juice method (A) indicates that individual differences due to work history are larger than expected in this complex contamination, and the number of remaining bacteria of the fingers after disinfection. Although there is a marked decrease in the number of people, there is also a reflection of individual differences in hand-washing techniques and complex contamination. In this hand washing disinfection test, there were some persons who showed the number of residual bacteria on the fingers after disinfection, and training was conducted on the hand washing technique of these persons, and rapid improvement was recognized.

[0047]

Example 3 Using a standard type of ozone water supply apparatus for hand washing and disinfection, a clinical test was carried out by a shower method using 30 seconds of pre-washing with a surfactant and 30 seconds of sterilization with ozone water. In this hand washing disinfection test, just before the test, the one-handed natural pollution degree test solution by the glove juice method (A)
Was collected and prewashed with a surfactant, and then hand-washed and disinfected with 4 ppm ozone water for 30 seconds. A test solution (B) for measuring the number of remaining bacteria was collected from the hand-washed ozone water waste liquid, and then the remaining ozone concentration (R ) Is measured. In order to determine the disinfecting effect, a test solution (C) for measuring the number of remaining bacteria is collected from the other hand after the hand washing and disinfection is completed by the glove juice method. These (A),
(B), (C) Transfer 1 mL of each test solution to each Petri dish,
Add Heart Infusion Agar Medium, 24 hours at 37 ° C.
After incubation for a period of time, the number of remaining bacteria is counted. When searching for bacterial species in colonies detected in culture, Staphylococcus aureus had the highest detection rate for natural contamination in hospitals. The panel asked a total of 26 nurses and doctors working in the hospital.
Table 6 shows the test results.

[0048]

[Table 6] The results in Table 6 show that individual differences or work histories are large in the natural pollution degree before disinfection, and that the actual pollution degree of the object to be disinfected is widely dispersed. It was confirmed in the above-mentioned clinical test that the method of the present invention using a surfactant works effectively even with such a wide variation in the contamination conditions, and shows a sufficient and sufficient disinfecting effect. That is, the results of these clinical tests are based on the basic experiments (in vitro) described in Experiment 1.
Experiment) and a good sign were confirmed. The method for rapidly disinfecting complex pollutants with ozone water according to the present invention is not limited to the prevention of infection in the medical field as described above, but also provides global benefits in terms of environmental pollution prevention.

[0049]

【The invention's effect】

(1) Since common contaminations of bacteria and various oxidizable substances are often observed in general disinfection targets, the conventional ozone water disinfection has been significantly inhibited. By applying the agent pre-cleaning method, its inhibitory action is remarkably suppressed, and effective sterilization and disinfection can be realized reliably and simply. (2) By greatly reducing the amount of ozone consumed by oxidizable contaminants adhering to the surface of the object to be disinfected, and improving the wettability of the surface of the object to be disinfected to increase the probability of contact with ozone water. In addition, the disinfecting effect of ozone water can be significantly improved. (3) Dramatically eliminate the deficiencies of conventional ozone water disinfection caused by complex contamination, greatly opening the way to the practical application of ozone water sterilization and disinfection, and making a great contribution to preventing the spread of infectious diseases. Therefore, application to biohazard countermeasures is also possible. (4) Applicable to commonly used simple disinfection methods such as showering, immersion, cleaning, spraying, wiping, etc., to achieve thorough sterilization and disinfection simply, quickly, safely, completely and efficiently. There is an advantage that the danger of bacterial diffusion by hand washing wastewater and the adverse effect on the environment due to dissolved ozone can be completely overcome. (3) Sterilization and disinfection of objects becomes more effective by adding a physical method such as ultrasonic irradiation. (4) By incorporating this method into a disinfection device, the entire disinfection procedure can be semi-automatically or fully automated, and the disinfection operation can be completed simply and reliably.

Claims (11)

[Claims] An ozone water disinfection method for a complex contaminated object in which an object to be disinfected that is complexly contaminated by the coexistence of a microorganism and an oxidizable substance and / or a substance such as an oily substance, a film substance, or a solid substance is disinfected with ozone water. The object to be disinfected is pre-washed with a surfactant to remove other contaminants that coexist with microorganisms, and then the object to be disinfected is disinfected with ozone water, and the microorganisms are disinfected with ozone water. A method for quickly disinfecting complex pollutants with ozone water, wherein the ability is effectively used, and a rapid and complete disinfection result is obtained with ozone water having a low concentration and a small dose. 2. The composite contaminated object according to claim 1, wherein an alkali salt of N-acyl-N-alkyltaurine, which consumes a small amount of dissolved ozone, or an agent effective therewith is selected as the surfactant. Quick disinfection method using ozone water. 3. An N-acyl-N-alkyltaurine alkali salt of a surfactant or the same effective agent is preferably 3 to 10 mL as an aqueous solution having a concentration of 1 to 20%, most preferably about 5%. The method for rapidly disinfecting a complex contaminated object with ozone water according to claim 1 or 2, characterized by using: 4. The concentration of ozone water for hand washing and disinfection is preferably 2 to 20 ppm, most preferably 3 to 5 ppm.
The method according to claim 1, wherein the amount of use is preferably 10 mL to 2 L, most preferably 500 mL to 1 L. 5. The ozone water for disinfecting an affected part or a wound,
Its concentration is preferably 10-25 ppm, most preferably 17-23 ppm, and the amount used is preferably 10 m
The method for quickly disinfecting complex contaminated objects with ozone water according to claim 1, wherein the amount is from L to 5L, most preferably from 500mL to 1L. 6. The concentration of ozone water for disinfecting equipment is as follows:
Preferably 25-70 ppm, most preferably 36-4
The method for rapidly disinfecting a complex contaminated object with ozone water according to claim 1, wherein the amount is 4 ppm, and the amount used is preferably 1 L to 20 L, most preferably 1 L to 5 L. 7. The combined pollutant according to claim 1, wherein the ozone water for disinfection of various bacteria, virus, rickettsiae, amoeba and the like can be completely killed at a concentration of 4 ppm. Quick disinfection method. 8. The method according to claim 1, wherein the concentration of the ozone water for disinfecting the amoeba having formed the cyst is set to 40 ppm or more. 9. The waste liquid used for disinfection is temporarily stored,
2. The ozone water of composite polluted matter according to claim 1, wherein the sterilization wastewater is discharged after detecting completion of sterilization by detection of excess presence of dissolved ozone, thereby satisfying the essential requirement of biosafety. Quick disinfection method. 10. The method according to claim 1, wherein the pre-cleaning with a surfactant and the disinfection with ozone water are performed using a semi-automatic or fully automatic apparatus. 11. The disinfection method using ozone water is such that the best disinfection result is always obtained by selecting a shower down method for hand washing disinfection and a dipping / washing method, a spray method or a wiping method for disinfecting objects. The method for rapidly disinfecting a complex contaminated object with ozone water according to claim 1, wherein: