CN113712028A

CN113712028A - Photoluminescent long-acting film-forming disinfection composition and preparation method and application thereof

Info

Publication number: CN113712028A
Application number: CN202011131820.6A
Authority: CN
Inventors: 王永禄; 李杨; 黄凯宇; 李学明; 王栋; 孙小虎; 陈卫
Original assignee: Nanjing Bestform Pharmtech Co ltd
Current assignee: Nanjing Bestform Pharmtech Co ltd
Priority date: 2020-10-21
Filing date: 2020-10-21
Publication date: 2021-11-30

Abstract

The invention provides a photoluminescence long-acting film-forming disinfection composition and a preparation method and application thereof. The product is sprayed on the surface of an object, is volatilized for 30 seconds, is completely solidified into a film in 5-10 minutes, continuously releases the medicine along with abrasion, has a disinfection effect lasting for 3-7 days, has good water resistance, can realize a long-acting antibacterial effect in a humid environment, and can be used on a faucet. The formed film has high transparency, can not be seen by naked eyes on the surfaces of common metal, plastic and wood after being sprayed, has no trace after being sprayed, and does not need subsequent cleaning. The product is also added with a photoluminescence fluorescent component, so that the visualization of a disinfection area can be realized under a common currency detecting lamp, and cleaning personnel can directly see the spraying range and the abrasion degree of the film, thereby facilitating the liquid supplementation. The product has high safety, no harm to human body and no corrosion to metal.

Description

Photoluminescent long-acting film-forming disinfection composition and preparation method and application thereof

Technical Field

The invention relates to a photoluminescence long-acting disinfection composition and a preparation method and application thereof.

Background

Direct or indirect contact with the surface of an object is one of the main routes for the transmission of microorganisms such as viruses and bacteria. The spreading way of the virus is cut off through the surface disinfection of objects in public places, and the protection of susceptible people becomes an important means for preventing and controlling epidemic infection. According to the disinfectant use guide published by the national Weijian Commission in 2 months 2020, all the classic disinfection methods are proposed to kill coronavirus, and intensive places such as hospitals, airports, public transport subway stations and the like, door handles, elevator buttons, handrails, toilet water taps and the like which are in high-frequency contact need to be cleaned and disinfected more frequently. This not only places high demands on the disinfection effort, but the demand for disinfection agents is also greatly increasing. The main problems of the prior common disinfectant in use are that the disinfection action time is short, the disinfection effect is difficult to last, and therefore, the disinfection needs to be repeated for many times. The disinfection frequency and the requirements of various public places are kept at a high standard by 10 months in 2020. The main problem of the conventional disinfectant at present is that the long-acting slow-release effect is not available, and the disinfection effect is difficult to ensure after the conventional disinfectant is disinfected 2 times a day. Therefore, the weight control departments such as the elevator room of the hospital and the like need to be disinfected four times a day and are matched with the disposable toilet paper keys.

If a long-lasting disinfectant product is provided, the utilization rate of the disinfectant can be greatly improved, and the workload of a disinfectant worker can be greatly reduced. At present, few reports of long-acting antibacterial disinfection products are available in the market, and the application range of the long-acting antibacterial disinfection products is greatly different from the specific requirements of disinfection of an elevator room. Products such as the CN103566371B patent technology

The water solution of the disinfectant is mainly suitable for long-term disinfection of clothes and fabrics, does not have long-term adhesive force after being sprayed on the surface of a hard object, and cannot play a long-acting antibacterial role on a smooth surface; patent CN111194750A discloses an inorganic salt disinfectant with film-forming binder, but the formulation contains inorganic salt compound of silver salt, copper salt, zinc salt, cerium salt and iron salt, which is expensive, and contains strong oxidant potassium permanganate, which is highly corrosive to metal and human body, and is not suitable for contact disinfection of armrests, elevator buttons and the like in public places with human body. In addition, the film-forming binder consists of polyvinyl alcohol, sodium carboxymethylcellulose and carbopol, and the water-soluble polyvinyl alcohol and the sodium carboxymethylcellulose account for 90% according to the combination proportion, so that the surface of the plaster cannot be ensured to keep a certain water resistance in the use process; patent CN111296476A discloses a long acting disinfectant with a color indicating disinfecting effect, but does not demonstrate the duration of the disinfecting effect in relation to the color indicator. In addition, the disinfection formula in the patent does not add an auxiliary agent which can assist the effective components to be attached to the surface to be disinfectedAnd is not conducive to long-term disinfection. In addition, buffers such as citric acid and malic acid are added in the formula, so that the metal surface can be corroded to a certain extent after long-term use. CN106538583A and CN 103992676B both adopt a disinfectant composite film forming technology, but the disinfection effect of the surface of an elevator button and a faucet which is continuously pressed and abraded by external force is not examined by the antibacterial effect, and users cannot visually judge the consumption degree of the disinfection product.

Disclosure of Invention

The invention mainly aims to provide a transparent wear-resistant, waterproof and visible disinfection composition with long-acting function. The disinfectant composition is prepared by curing a disinfectant and a photoluminescent agent on a surface to be disinfected by mainly using a high-molecular film-forming material with strong adhesion and good ductility in a formula as a carrier. Meanwhile, different film forming materials are compounded to obtain a transparent film with good wear resistance and waterproof effect, so that the lasting effect of the disinfectant is ensured, the appearance of the surface to be disinfected is not influenced, and the monitoring of a sanitation worker on a disinfection area can be assisted.

The disinfection composition provided by the invention is mainly applied to a surface to be disinfected in a spraying mode, and can promote quick film formation by virtue of quick volatilization of an organic solvent, and the disinfection composition specifically comprises the following components:

A. film-forming material composition

The long-acting effect of the disinfection composition of the invention should exert the same disinfection effect under different climatic conditions and different contact conditions, so that the disinfection composition should have certain wear resistance and water resistance after film formation to ensure the integrity of the film, but at the same time the disinfection composition should be capable of removing the film agent for re-disinfection in a convenient and simple manner for disinfection workers. According to the requirements for the film-forming material of the disinfection composition, the film-forming material composition is obtained by screening various high polymer materials with different properties in terms of type, dosage and compounding effect, and specifically comprises one or a mixture of a fat-soluble high polymer material and a hydrophilic high polymer material, wherein the hydrophilic high polymer material further comprises a water-soluble polymer and a non-ionic high polymer binder. The mass ratio of the fat-soluble high polymer material to the hydrophilic high polymer material is 1-5: 5; the mass ratio of the water-soluble polymer to the nonionic high-molecular binder is 1-3: 2.

The fat-soluble high polymer material can be one or more of polyurethane, polyacrylate, polyvinyl butyral, methyl cellulose and ethylene-vinyl acetate copolymer.

The water-soluble polymer further comprises one or more of polyethylene glycol, polyvinyl alcohol, polyacrylamide and hydroxypropyl methylcellulose.

The non-ionic high-molecular binder further comprises one or more of polyvinylpyrrolidone, cross-linked polyvinylpyrrolidone, hydroxypropyl methylcellulose, gelatin, acacia and sodium alginate.

B. Photoluminescent agent

The invention provides a more efficient long-acting disinfectant for cleaning personnel, the disinfectant is transparent, can not be seen on the surface of an object by naked eyes, and therefore, the appearance of the surface of the object can not be influenced, and the cleaning at a later stage are not needed. For example, when used to disinfect elevators in hospitals and malls, the flow of people fluctuates greatly at different times and places, and the effective action time of the long-acting disinfection membrane also varies greatly. The invention adopts the way that the photoluminescence fluorescence indicating component is added into the disinfection composition, thereby solving the visualization problem of the disinfection area. The research also confirms that the photoluminescence agent and the disinfectant can form a cocrystal after the disinfection solution is formed into a film through a microscope and a Scanning Electron Microscope (SEM), and the structural distribution of the cocrystal in the film is represented through the SEM (shown in figures 1-3). Based on the cocrystallization formed by the disinfectant and the photoluminescent agent, when the disinfectant is reduced along with the increase of the number of touch times, the photoluminescent agent is reduced, so that the process can be monitored conveniently by using a common currency detecting lamp as an auxiliary tool. The visible photoluminescence indicator in the invention can specifically select water-soluble or fat-soluble fluorescent dye. The water-soluble fluorescent dye comprises but is not limited to fluorescent brightener BC, fluorescent brightener JD-3, fluorescent brightener BR, fluorescent brightener EBF and fluorescent brightener CBS-X; the fat-soluble fluorescent dye includes but is not limited to fluorescent whitening agent OB, fluorescent whitening agent OB-1, fluorescent whitening agent KCB and fluorescent whitening agent KSB.

C. Quaternary ammonium salt disinfectant

The disinfectant composition is a product with long-acting disinfection, and considering that the disinfectant is stored on the surface of an object for a long time, such as the surfaces of an elevator button, a handrail, a door handle and the like, and the main contact part is on the hand, the disinfectant in the invention selects a quaternary ammonium salt disinfectant with higher safety and lower toxicity to the human body, and further can select one or more of benzalkonium chloride, benzalkonium bromide, benzethonium chloride, didecyldimethylammonium chloride, dodecyltrimethylammonium chloride and the like.

D. Plasticizer

In order to further improve the ductility of the film formed by the disinfection composition, the formula is added with a plasticizer, and further comprises one or more of polyethylene glycol, glycerol, propylene glycol and the like.

E. Flavoring agent

In consideration of the application scene of the disinfection composition, which relates to an elevator and other closed spaces, in order to improve the adverse effect on the ambient air, the formula is added with a flavoring agent, and the disinfection composition further comprises fragrance components such as camphor, vanillin, mint fragrance, nerol, citrus fragrance, citronellal and the like.

F. Organic solvent

The organic solvent can be one or more selected from acetone, ethyl acetate, isopropanol, diethyl ether and ethanol.

The specific preparation method of the disinfection composition can be directly prepared at room temperature by a conventional physical mixing method without matching special environmental conditions, and the process adopts a similar intermiscibility principle to respectively obtain a uniform organic phase and a uniform water phase, and then the uniform organic phase and the uniform water phase are mixed. The preparation process comprises the following steps:

a. dissolving a hydrophilic polymer material, a water-soluble fluorescent agent and a water-soluble flavoring agent in purified water in sequence to obtain a solution A;

b. dissolving a quaternary ammonium salt disinfectant, a fat-soluble flavoring agent, a fat-soluble fluorescent agent and a fat-soluble high polymer material in an organic solvent in sequence to obtain a solution B;

c. mixing solution A and solution B, adding plasticizer and flavoring agent, mixing and stirring for 1-24 hr.

It is another object of the present invention to provide the use of said disinfecting composition.

The application of the disinfection composition provided by the invention is to disinfect the surface of frequently-used facilities in public places, and is particularly suitable for elevator room buttons, door handles, handrails, water taps and the like in public places such as shopping malls, hotels, hospitals, schools, public transportation subways and the like with highly concentrated crowds. The product is also suitable for surface disinfection of living goods such as mobile phones, children toys and the like.

The specific application mode of the disinfection composition is that after the disinfection solution is sprayed on the surface of an object, an auxiliary tool capable of emitting invisible ultraviolet light is adopted to irradiate the area covered by the disinfection solution, and the spraying range or the spraying amount is properly increased according to the reflected fluorescence range and intensity.

The disinfection composition realizes long-acting disinfection through the film forming substrate, and can maintain the continuous disinfection effect for 3-10 days by adopting different formula proportions without the influence of artificial strong wiping or other special factors.

The product form of the disinfection composition is not limited to solution, spray and disinfection film agent prepared by taking the disinfection composition as a main component.

Aiming at the actual market demands of long-acting, slow-release and wear-resistant disinfectants, the invention develops a photoluminescent long-acting slow-release high-molecular polymer long-acting wear-resistant disinfectant. Compared with the existing long-acting disinfection products, the product has remarkable advantages in the design invention.

The product successfully solves the problem of the synergistic effect of the wear resistance and the antibacterial effect of the product. The patents CN111194750A, CN106538583A and CN 103992676B all use film-forming materials in disinfection products, can realize the long-acting disinfection effect of the surface of a common object, but the product does not consider the problem of the synergistic effect of wear resistance and antibacterial effect in the design. If the film material is pursued to have wear resistance in the past, the disinfection component in the material is difficult to release and take effect, the effect is equivalent to that the disinfection component on the surface of an object is covered by a plastic film, and the disinfection effect is lost when the disinfection component on the surface of the film is worn. Ideally, as the membrane material is gradually worn, the disinfection component in the membrane material can be continuously exposed and released, and the long-acting disinfection effect is realized.

The product successfully realizes the unification of the transparency and the wear resistance of the film. If the product is widely accepted, the film needs to be transparent, so that people cannot feel that the disinfectant is sprayed, otherwise, the original surface cleanliness of the object is influenced, and no people are willing to use the product. The method of patent CN111296476A for showing the disinfection effect by color is obviously not preferable. In the research and development process of the patent, the transparency and the wear resistance of the film are difficult to ensure at the same time, if the wear resistance is improved, the transparency is reduced, the surface of an object is easy to have dirty feeling, and cleaning by cleaning personnel is not facilitated; conversely, increasing transparency reduces the abrasion resistance of the film and does not guarantee a durable disinfecting capacity. The current product formula successfully solves the problem of the contradiction, and improves the wear resistance while ensuring the transparency of the film.

The invention aims to add the photoluminescent material in the prescription and also aims to solve the problem that the film of the product has high transparency and is invisible to naked eyes. After the fluorescent material is added, a cleaner can realize the visualization of the disinfection range and the abrasion degree by means of a tool for emitting invisible ultraviolet light, such as a common currency detecting lamp, so that the use frequency of the disinfectant can be effectively reduced, and the waste is avoided.

The ideal film should also be suitable for the influence of high humidity weather and human sweat, and has a certain waterproof property, and the film forming materials used in patents CN111194750A and CN 103992676B are mainly film forming water-soluble or water-dispersible materials, so that the waterproof effect of the product is difficult to realize. Through multiple experiments, the formula proportion and components are continuously improved, the problem of uniform wear resistance, light transmittance and water resistance is finally successfully solved, the transparent wear-resistant, waterproof and visible long-acting disinfectant is developed, and the long-acting disinfection effect on the faucet can be realized.

Has the advantages that: the photoluminescence long-acting film-forming disinfection composition has the advantages that:

1. long-acting slow-release disinfection. The product is sprayed on the surface of an object, is volatilized for 30 seconds and is completely solidified into a film within 5-10 minutes. After being touched by a hand, the bacteria and viruses infected on the film are quickly killed by the inner disinfectant enveloped in the film, and the film can continuously release the medicines along with gradual abrasion, so that a long-acting disinfection effect is realized, taking an elevator cabin button as an example, the film can bear 5000 + 10000 pressing after being formed, and the disinfection effect can last for 3-7 days according to different use frequencies of elevators. The use frequency of the common disinfectant can be effectively reduced, and the working efficiency is greatly improved while the input cost is reduced.

2. The invention selects the cationic quaternary ammonium salt compound with higher safety as the disinfection component, the disinfectant has low toxicity, safety, small side effect, low concentration, no color, no odor and low irritation, the disinfection principle is that the disinfectant is adsorbed on the surface of bacteria with negative charges to change the permeability of a bacterial cell serous membrane and break thalli, thereby playing a role in killing, mainly having a physical effect and having no selectivity on the form and the type of the bacteria, so that the disinfection effect of the quaternary ammonium salt compound is not influenced by the variation of the bacteria, and the bacteria can not generate drug resistance.

3. The film formed by the product has high transparency, can not be seen by naked eyes on the surfaces of common metal, plastic and wood after being sprayed, has no trace after being sprayed, and does not need subsequent cleaning.

4. Because the product can not be seen by naked eyes, the product is added with a photoluminescence fluorescent component in order to conveniently judge the abrasion condition of the disinfection film. After the photoluminescence fluorescent component is added, the disinfection film can realize the visualization of a disinfection area under a common currency detecting lamp, a cleaner can visually see the abrasion degree of the film, and the liquid supplementing is convenient. Scanning electron microscope results show that the disinfectant and the fluorescent agent are uniformly distributed in the disinfection film in a cocrystallization mode, and the fluorescent agent can well indicate the existence of the disinfectant.

5. The water resistance is good, the long-acting antibacterial effect under the humid environment can be realized, and the water faucet can also be used.

6. The product has high safety, no corrosion to metal, and no harm to human body. No acidic, basic or strong oxidant component is added in the formula, so that the metal surface is non-corrosive, and the metal surface disinfectant is particularly suitable for disinfecting the surface of an object.

Drawings

Photoluminescent agent-disinfectant cocrystals observed microscopically in fig. 1, A, B: microscopic observation of cocrystals under the same visual field, a: co-crystals of a photoluminescent agent-disinfectant under an LED light source; b: the currency detecting lamp irradiates the picture A to display that a blue fluorescent agent appears in the cocrystal;

FIG. 2 surface structure SEM characterization after film formation of the disinfectant solution, A: the film-forming surface structure only contains disinfectant solution formula; b: the solution formula containing only the photoluminescence agent forms a film surface structure; c: forming a film surface structure by a solution formula containing a disinfectant and a photoluminescence agent;

FIG. 3 Cross-sectional SEM characterization of the disinfecting solution after film formation, A, B: a disinfectant-only film; C. d: a film containing only a photoluminescent agent; E. f: a disinfectant-photoluminescent agent-containing film;

FIG. 4 results of abrasion resistance investigation;

fig. 5 visual effect of comparative example 2 and example 4, A, B: appearance under daily conditions; C. d: and (5) observing the view of the microscope under the irradiation of the currency detecting lamp.

Detailed Description

The present invention will be described below with reference to specific examples, but the present invention is not limited to these examples. It will be apparent to those skilled in the art that various modifications and improvements can be made to the present invention without departing from the spirit of the invention, and these modifications and improvements fall within the scope of the claims of the present invention.

The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, biomaterials, etc. used in the following examples are commercially available unless otherwise specified.

Example 1

The formula is as follows:

preparation:

a. taking purified water, dissolving polyacrylamide, gelatin and a water-soluble fluorescent whitening agent in sequence, and uniformly stirring to obtain solution A;

b. dissolving polyvinyl butyral, benzalkonium bromide and camphor in sequence by using absolute ethyl alcohol to obtain solution B;

c. and mixing the solution A and the solution B, stirring uniformly, adding glycerol, and continuously stirring for 24 hours to obtain the liquid.

Comparative example 1

A disinfectant composition was prepared in the same manner as in example 1.

The formula is as follows:

A. film-forming material composition	20g/L
		Polyacrylamide	10g/L
Gelatin	10g/L
		B. Fluorescent whitening agent CBS-X	0.6g/L
C. Benzalkonium bromide	2g/L
		D. Glycerol	10g/L
E. Camphor	1g/L
		F. Ethanol	500g/L
G. Purified water	Balance of

Investigation of Water resistance test

Taking a flat-bottom evaporation pan with the diameter of 40mm, pouring the disinfection composition solution prepared in the example 1 and the disinfection composition solution prepared in the comparative example 1 into the evaporation pan respectively, putting the evaporation pan into a reduced pressure drying oven with the temperature of 37 ℃ for drying until the weight is constant, taking out a film obtained after the solvent in the evaporation pan is volatilized, cutting the film into the size of 20mm multiplied by 20mm, putting the film into water for soaking for a certain time, taking out the film, and drying the film again until the weight is constant. The mass of the film dried to constant weight before and after soaking was weighed (W)₀And W₁) And the mass loss ratio (W%) before and after the film immersion was calculated, the smaller W%, indicating the better water resistance of the film. The mass loss ratio (W%) was calculated as shown below.

W％＝[(W₁-W₀)/W₀]×100％

TABLE 1 Water resistance test results

As can be seen from the results of the water resistance test in Table 1, the water resistance test in example 1 has a good water resistance effect, the mass loss ratio of the sample soaked in 40 times of volume of water for 24 hours is close to 50%, the residual film structure is basically complete, and the film still has a certain wear resistance after being dried. In contrast, comparative example 1 was completely dissolved within 1 hour under the same conditions.

Example 2

The formula is as follows:

A. film-forming material composition	45g/L
		Polyurethane	20g/L
Polyvinyl alcohol	15g/L
		Polyvinylpyrrolidone	10g/L
B. Fluorescent lampOptical brightener OB	0.8g/L
		C. Benzalkonium bromide	1g/L
D. Polyethylene glycol 400	20g/L
		E. Citrus incense	0.7g/L
G. Acetic acid ethyl ester	750g/L
		H. Purified water	Balance of

Preparation:

a. taking purified water, dissolving polyvinyl alcohol and polyvinylpyrrolidone in sequence, and stirring uniformly to obtain solution A;

b. dissolving benzalkonium bromide, citrus fragrance, fat-soluble fluorescent whitening agent and polyurethane in turn by using ethyl acetate to obtain solution B;

c. and mixing the solution A and the solution B, stirring uniformly, adding polyethylene glycol 400, and continuously stirring for 24 hours to obtain the liquid medicine.

Investigation of abrasion resistance test

Considering that the disinfecting composition of the present invention is mainly applied to the surface of an object frequently touched, vigorous wiping or application of gravity is not suitable for verifying the abrasion resistance in practical use thereof. In order to examine the wear resistance, the initial mass (W) of the evaporating dish was first determined₀) Then, the solution of the disinfecting composition was poured into a flat-bottom evaporating dish, dried under reduced pressure at 37 ℃ to form a film, and the initial mass (W) of the film + evaporating dish was measured_i) And the total mass (W) of the membrane and the evaporating dish after pressing is measured after pressing the membrane for 1000, 3000, 5000 and 10000 times by simulating the mode that a finger presses a button of the elevator_n) The abrasion resistance of the film was evaluated by calculating the mass loss (W%) ratio of the film before and after pressing.

Calculating the formula: w% ((W))_i-W_n)/(W_i-W₀) Fig. 4 shows the results of the wear resistance test.

From the results of fig. 4, it is understood that in example 2, the film morphology remained intact after pressing 10000 times, the film mass loss was 30% or less, and the abrasion resistance was good. Comparative example 1 also had better abrasion resistance, with no significant difference.

Example 3

The formula is as follows:

A. film-forming material composition	50g/L
		Polyurethane	10g/L
Polyvinyl alcohol	20g/L
		Hydroxypropyl methylcellulose	20g/L
B. Fluorescent whitening agent JD-3	0.3g/L
		C. Benzalkonium chloride	2g/L
D. Polyethylene glycol 400	15g/L
		E. Menthol crystal	0.5g/L
F. Ethanol	800g/L
		G. Purified water	Balance of

Preparation:

a. dissolving polyvinyl alcohol, hydroxypropyl methylcellulose and a water-soluble fluorescent whitening agent in purified water in sequence, and uniformly stirring to obtain a solution A;

b. dissolving benzalkonium chloride, menthol and polyurethane in sequence by using absolute ethyl alcohol to obtain solution B;

c. and mixing the solution A and the solution B, stirring uniformly, adding polyethylene glycol 400, and continuously stirring for 12 hours to obtain the liquid medicine.

Sterilization test of the disinfecting composition

The disinfecting composition of example 3 of the present invention was tested for its microbicidal effect according to the Disinfection Specification (2002 edition).

The disinfectant solution of the present invention is mainly used in public places, and thus common escherichia coli and staphylococcus aureus were selected as test strains.

Test strain name: escherichia coli, strain number: 8099, staphylococcus aureus, strain number: ATCC6538, the passage numbers of the above strains are: fifth generation, strain source: ATCC (American ginseng).

Quantitative microorganism killing test

The inspection basis is as follows: disinfection Specification (2002 edition by Ministry of health) 2.1.1.5, 2.1.1.7.

Quantitative identification test of neutralizer suspension: the following tests were carried out according to the specification for disinfection (2002 edition) 2.1.1.5 neutralizer identification test, and the components of bacteria identification neutralizer: 3% tween 80, 2% lecithin, nutrient broth.

And (3) checking the ambient temperature: 26 ℃, relative humidity: 48 percent.

TABLE 2 test results for quantitative identification of neutralizer suspension

TABLE 3 quantitative kill test results for suspensions

From the results, the killing logarithm value of the product on escherichia coli (8099) and staphylococcus aureus (ATCC6538) is more than 5.00, and the sterilization rate within 5min of the shortest action time can reach 99.999 percent, which shows that the product has instant sterilization efficiency and excellent sterilization effect and meets the requirements of the sterilization technical specification (2002 edition of Ministry of health).

(II) Long-term antibacterial test

The detection method comprises the following steps: uniformly coating the disinfectant on a sterilized 2cm × 3cm cloth piece according to a ratio of 5 ml/piece, drying at room temperature for later use, and taking the sterilized normal saline as a test group, uniformly coating the sterilized 2cm × 3cm cloth piece according to a ratio of 5 ml/piece, and drying at room temperature for later use as a positive control group. The two groups of carriers are stored in a sterile laboratory at room temperature, after 7 days of storage, the test group and the positive control group are taken out, the freshly cultured bacterial suspension is stained, 100.0 mu l of the suspension is respectively dripped into the test group and the positive control group, the two groups of carriers are uniformly mixed, and timing is started. After the mixture is acted for 7.5 minutes, 15 minutes and 22.5 minutes, 0.5ml of the mixed solution of the test bacteria and the disinfectant is respectively sucked and added into 5.0ml of the sterilized neutralizer, and the mixture is evenly mixed. After the neutralizing agent acts on the mixed liquid of the test bacteria and the disinfectant for 10min, 1.0ml of sample liquid is respectively absorbed and diluted, 2-3 dilutions are respectively absorbed, 1.0ml of sample liquid is respectively absorbed and placed in 2 plates, the plates are rotated by a culture medium cooled to 45 ℃, the culture medium is fully and uniformly stirred, the plates are turned over after agar is solidified, and the culture medium is cultured for 48h at 36.0 ℃ to count viable bacteria colonies. The experiment was repeated 3 times.

And (3) checking the ambient temperature: 21 ℃, relative humidity: 40 percent.

TABLE 4 Long-term antibacterial test results

The long-acting antibacterial test simulates whether the product has long-acting antibacterial effect on the disinfected surface after film forming in actual use. From the results, the sterilization rate of the product on escherichia coli exceeds 99.9% after the product is placed for 7 days after being filmed, the sterilization rate on staphylococcus aureus reaches 99%, the product meets the requirements of sterilization technical specification (2002 edition of Ministry of health) on the antibacterial efficiency (90%) of an antibacterial agent, and the product has the advantage of long-acting antibiosis.

Example 4

The formula is as follows:

A. film-forming material composition	50g/L
		Polyacrylate	15g/L
Polyethylene glycol	20g/L
		Crosslinked polyvinylpyrrolidone	15g/L
B. Fluorescent whitening agent JD-3	0.9g/L
		C. Didecyl dimethyl ammonium chloride	1.5g/L
D. Propylene glycol	10g/L
		E. Citrus incense	0.3g/L
F. Acetone (II)	500g/L
		G. Purified water	Balance of

Preparation:

a. dissolving polyethylene glycol, crosslinked polyvinylpyrrolidone and water-soluble fluorescent whitening agent in purified water in sequence, and stirring uniformly to obtain solution A;

b. taking acetone, and dissolving didecyl dimethyl ammonium chloride, citrus fragrance and polyacrylate in sequence to obtain solution B;

c. and blending the solution A and the solution B, stirring uniformly, adding propylene glycol, and continuously stirring for 2 hours to obtain the liquid.

Comparative example 2

A disinfecting composition was prepared in the same manner as in example 4, except that 0.09% of a water-soluble fluorescent whitening agent was not added to the formulation, and the remaining ingredients and amounts were the same as in example 4.

Visual effect contrast test

After the disinfecting compositions prepared in example 4 and comparative example 2 were sprayed onto the surface of an object, the sprayed area was irradiated with a bill check lamp, and the phenomenon was observed. In addition, since 2 disinfecting compositions formed a film invisible to the naked eye, it was not convenient to visually compare the visual differences brought about by the fluorescent agent. Therefore, the solutions of the disinfecting compositions of example 4 and comparative example 2 were then evaporated to dryness in a flat-bottom evaporation dish to form a film, and the visualization of the two films was compared. The films of the disinfectant compositions of example 4 and comparative example 2 were irradiated by a money-detecting lamp, and the difference in color exhibited by the two films was observed, and the result is shown in fig. 5.

TABLE 5 statistical tables of film formation times of examples 1 to 4

Sample (I)	Solvent volatilizing time	Curing film-forming time
			Example 1	25.2s	8.0min
Example 2	27.8s	7.8min
			Example 3	25.5s	7.4min
Example 4	23.4s	8.0min

As shown in the results in Table 5, the product was volatilized in 30 seconds and completely solidified into a film in 5 to 10 minutes.

Claims

1. A photoluminescent long-acting film-forming disinfecting composition, characterized by: the components and the contents of the disinfection composition are as follows:

the film-forming high polymer material composition is a mixture of a fat-soluble high polymer material and a hydrophilic high polymer material, and the mass ratio of the fat-soluble high polymer material to the hydrophilic high polymer material is (1-5): 5;

the disinfection composition is quickly volatilized after being sprayed on the surface to be disinfected within 0.5 to 3 minutes, and is solidified into a transparent film which can not be seen by naked eyes within 5 to 10 minutes.

2. The photoluminescent long-acting film-forming disinfecting composition of claim 1, wherein the photoluminescent agent is a water-soluble or lipid-soluble fluorescent dye capable of forming a cocrystal with the quaternary ammonium disinfectant.

3. The photoluminescent long-acting film-forming disinfecting composition of claim 1, wherein the quaternary ammonium salt disinfectant is selected from one or more of benzalkonium chloride, benzalkonium bromide, sodium hypochlorite, benzethonium chloride, didecyldimethyl ammonium chloride and dodecyltrimethyl ammonium chloride.

4. The photoluminescence long-acting film-forming disinfection composition as claimed in claim 1, wherein the plasticizer can be one or more selected from polyethylene glycol, glycerol and propylene glycol; the flavoring agent can be one or more of camphora, vanillin, Mentha haplocalyx, nerol, Mandarin, and citronellal.

5. The photoluminescence long-acting film-forming disinfection composition as claimed in claim 1, wherein the organic solvent is selected from one or more of acetone, ethyl acetate, isopropanol, ether and ethanol.

6. The photoluminescent long-acting film-forming disinfecting composition of claim 1, wherein the hydrophilic polymeric material comprises a water-soluble polymer and a non-ionic polymeric binder; the dosage ratio of the water-soluble polymer to the nonionic polymer binder is 1-3: 2.

7. The photoluminescence long-acting film-forming disinfection composition as claimed in claim 1, wherein the fat-soluble polymer material is selected from one or more of polyurethane, polyacrylate, polyvinyl butyral, methyl cellulose and ethylene-vinyl acetate copolymer.

8. The photoluminescence long-acting film-forming disinfection composition as claimed in claim 6, wherein the mass ratio of the water-soluble polymer to the non-ionic high-molecular binder is 1-3: 2; the water-soluble polymer is one or more of polyethylene glycol, polyvinyl alcohol, polyacrylamide and hydroxypropyl methylcellulose; the non-ionic polymer binder is one or more of polyvinylpyrrolidone, cross-linked polyvinylpyrrolidone, hydroxypropyl methylcellulose, gelatin, acacia and sodium alginate.

9. A method of preparing a photoluminescent long-acting film-forming disinfecting composition according to any one of claims 1 to 8, comprising the steps of:

a. dissolving hydrophilic polymer material, water-soluble photoluminescence agent and hydrophilic flavoring agent in purified water to obtain solution A;

b. dissolving quaternary ammonium salt disinfectant, fat-soluble photoluminescence agent, fat-soluble high polymer material and lipophilic flavoring agent in an organic solvent to obtain solution B;

c. mixing the solution A and the solution B, adding a plasticizer, and mixing and stirring for 1-24 h.

10. Use of a photoluminescent long-acting film-forming disinfecting composition according to any one of claims 1 to 8 for disinfecting surfaces of hard objects.