CN104938515A - Method for inhibiting the sprouting and growth of weeds - Google Patents
Method for inhibiting the sprouting and growth of weeds Download PDFInfo
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- CN104938515A CN104938515A CN201510387876.0A CN201510387876A CN104938515A CN 104938515 A CN104938515 A CN 104938515A CN 201510387876 A CN201510387876 A CN 201510387876A CN 104938515 A CN104938515 A CN 104938515A
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Abstract
The invention provides a method for inhibiting the sprouting and growth of weeds. The method for inhibiting the sprouting and growth of plants and roots of weeds is characterized in that effective dose of geraniol is given. According to the method for inhibiting the sprouting and growth of the weeds, through the operation that six types of allelopathy components of different concentration are used for treating pigweed seeds, it is founded that one mM of the geraniol has the most evident effect on inhibiting the sprouting and growth of the pigweeds, and the inhibition ratio reaches 49.97%. It can be shown that the one mmol/L of the geraniol has a wide application prospect on weed control, in particular to the respect of the control over dicotyledon weeds.
Description
Technical field
The invention belongs to allelopathy field, be specifically related to a kind of method suppressing weed germination and plant height and root long.
Background technology
Farmland weed brings harm to the g and D of crop, and a large amount of chemical herbicide that uses can cause very serious grain security and problem of environmental pollution.The allelopathy of plant refers to the relation that counteracts each other that plant is set up with environmental organism by the release of secondary metabolites.Research shows, some species can suppress sprouting and the growth of association species by release allelochemical.Utilizing allelopathy to carry out Weeds distribution is exactly develop biopesticide on the basis studying suppression mechanism mutually between natural world species, thus realizes the BIOLOGICAL CONTROL of weeds, is the Weeds distribution mode of a kind of worth exploration, safety.
Fragrant plant is the biological group that a class has vigorous secondary metabolism, and the Aromatic plant resources of China enriches.There are some researches show, some aromatic species have significant Water extract effect to weeds, and as Asia thyme can suppress green foxtail seed germination and growth of seedling, holy basil has significant inhibitory action to weeds three-coloured amaranth.But still have the allelopathic of a lot of fragrant plant to press down grass effect and have no report, the Screening germplasm that therefore fragrant plant suppresses weeds has very large research space.
Allelopathic Effect in Plants is by realizing to Environment release allelochemical.Plant can discharge many kinds of allelochemicals under field conditions (factors), and allelochemical enters soil by leaching by precipitation, has an impact to biotic habitat.Therefore, the ecological effect studying allelochemical has important theory and practice meaning for the exploitation of deep announcement Allelopathic Effect in Plants mechanism and biological weed killer.
Summary of the invention
In order to solve the problem, the invention provides a kind of method suppressing weed germination and plant height and root growth.
A kind of method suppressing weed germination and plant height and root growth provided by the invention, is characterized in that, give the geraniol of effective dose.
In one embodiment of the invention, described weeds are preferably broadleaf weed, are more preferably the grey dish in broadleaf weed.
In one embodiment of the invention, described geraniol is liquid preparation, and its concentration is 1mmol/L.
The present invention by comparing the impact of 6 kinds of allelopathic component variable concentrations water extracts on grey dish seed germination and growth, find 1mmol/L geraniol grey dish is sprouted and the inhibitory action of growth the strongest.Inhibiting rate reaches 49.97%.Show that the geraniol of 1mmol/L is in control weeds, especially control broadleaf weed aspect and have broad application prospects.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 geraniol is sprouted and growth inhibiting remarkable impact for grey dish
Geraniol sprouts grey dish and the remarkable impact of growth screens from the variable concentrations of 6 kinds of allelochemical sterlings.
1. the preparation of allelochemical sterling solution
The geraniol aqueous solution of preparation 15 concentration, being respectively 1mmol/L, 0.8mmol/L, 0.6mmol/L, 0.5mmol/L, 0.4mmol/L, 0.2mmol/L, 0.1mmol/L, 0.08mmol/L, 0.06mmol/L, 0.05mmol/L, 0.04mmol/L, 0.02mmol/L, 0.01mmol/L, 0.005mmol/L, 0.001mmol/L, take clear water as blank.
The o-coumaric acid aqueous solution of preparation 9 concentration, being respectively 1mmol/L, 0.5mmol/L, 0.1mmol/L, 0.05mmol/L, 0.01mmol/L, 0.005mmol/L, 0.001mmol/L, 0.0005mmol/L, 0.0001mmol/L, take clear water as blank.
The caffeic acid aqueous solution of preparation 10 concentration, being respectively 1mmol/L, 0.75mmol/L, 0.5mmol/L, 0.25mmol/L, 0.1mmol/L, 0.05mmol/L, 0.01mmol/L, 0.001mmol/L, 0.0003mmol/L, 0.0001mmol/L, take clear water as blank.
The forulic acid aqueous solution of preparation 10 concentration, be respectively 1mmol/L, 0.8mmol/L, 0.6mmol/L, 0.5mmol/L, 0.4mmol/L, 0.2mmol/L, 0.1mmol/L, 0.05mmol/L, 0.01mmol/L, 0.005mmol/L take clear water as blank.
The vanillin aqueous solution of preparation 12 concentration, being respectively 1mmol/L, 0.5mmol/L, 0.1mmol/L, 0.08mmol/L, 0.06mmol, 0.05mmol, 0.04mmol/L, 0.02mmol/L, 0.01mmol/L, 0.005mmol/L, 0.0025mmol/L, 0.001mmol/L, take clear water as blank.
The aqueous solution of salicylic acid of preparation 11 concentration, is respectively 1mmol/L, 0.8mmol/L, 0.6mmol/L, 0.5mmol/L, 0.4mmol/L, 0.2mmol/L, 0.1mmol/L, 0.05mmol/L, 0.01mmol/L, 0.005mmol/L, 0.008mmol/L.
2. the process of grey colza
Select full grains, neat and consistent grey colza with 5% NaClO to sterilize 3-5min, with tap water several, finally use distilled water flushing 3-5 time for subsequent use.
3. the Weed-suppressive effect of allelochemical detects
Adopt culture dish filter paper method, add the treatment fluid to be measured of 3ml different component in the 7cm culture dish being covered with double-layer filter paper, contrast adds the distilled water of equivalent.Get the full confession of 30 grain length gesture to plant experimentally son and be evenly placed in culture dish, randomized complete-block design is taked in test, and 3 repetitions are established in each process, are placed in by culture dish on culturing rack, at 25 DEG C, dark (covering gobo) cultivates, and within 7 days, measures root length and the plant height of grey dish afterwards.
(1) measurement index
Germination rate: the standard of sprouting is that radicle breaks through seed coat 1-2mm.
Root is long: collar position is long as root to tip of a root position.5 strain seedling got at random by each culture dish, and 3 culture dishes are got in each process altogether.Totally 15 strain seedling, long with the ruler mensuration root measured as 20cm.
Plant height: using the length at the above position of collar as plant height.5 strain seedling got at random by each culture dish, and 3 culture dish 15 strain seedling are got in each process altogether, measure plant height with the ruler measured as 20cm.
Germination rate=(in the sprouting number/culture dish of seed seed sum) × 100%
Long (plant height) inhibiting rate of root: quote Williamson etc. and once appointed gloomy method to calculate plant height inhibiting rate.Formula is: IR=(T
0-T
1) × 100/T
0, wherein T
1for the processing costs of test event, T
0for control value.Represent to there is depression effect as IR>0, during IR<0, represent to there is Promotive effect.The power of the absolute value representation allelopathy of IR.
Allelopathic comprehensive effect index (SE): be sprout, the arithmetic mean of instantaneous value of the contrast inhibition percentage of long and plant height 3 testing indexs of root, be evaluate allelochemical to sprout plant and the parameter of growth entire effect.
(2) result of the test
The geraniol of table 1 variable concentrations is sprouted for grey dish, the long impact suppressed of plant height and root
The o-coumaric acid of table 2 variable concentrations is sprouted for grey dish, the long impact suppressed of plant height and root
The caffeic acid of table 3 variable concentrations is sprouted for grey dish, the long impact suppressed of plant height and root
The forulic acid of table 4 variable concentrations is sprouted for grey dish, the long impact suppressed of plant height and root
The vanillin of table 5 variable concentrations is sprouted for grey dish, the long impact suppressed of plant height and root
The salicylic acid of table 6 variable concentrations is sprouted for grey dish, the long impact suppressed of plant height and root
As can be seen from table 1-6,6 kinds of allelochemicals are all the strongest to the comprehensive depression effect (SE) of grey dish 3 indexs when concentration is 1mmol/L, wherein the SE of geraniol to grey dish of 1mmol/L is the strongest, compare with contrast, inhibitory action reaches 49.97%, is the component that in all process element, Water extract effect is the strongest.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (4)
1. suppress a method for weed germination and growth, it is characterized in that, give the geraniol of effective dose.
2. the method for claim 1, is characterized in that, described weeds are broadleaf weed.
3. method as claimed in claim 2, it is characterized in that, described weeds are grey dish.
4. the method as described in any one of claim 1-3, is characterized in that, described geraniol is liquid preparation, and its concentration is 1mM.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510387876.0A CN104938515B (en) | 2015-07-03 | 2015-07-03 | A kind of method for suppressing weed germination and growth |
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|---|---|---|---|
| CN201510387876.0A CN104938515B (en) | 2015-07-03 | 2015-07-03 | A kind of method for suppressing weed germination and growth |
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| CN104938515B CN104938515B (en) | 2017-12-01 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106883047A (en) * | 2017-04-08 | 2017-06-23 | 叶长东 | Ratoon sugarcane cultivates fertilizer |
| CN116472818A (en) * | 2023-04-12 | 2023-07-25 | 石河子大学 | Method for promoting ragweed seed germination |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000051436A1 (en) * | 1999-03-05 | 2000-09-08 | Ecosmart Technologies, Inc. | Herbicidal compositions containing plant essential oils and mixtures or blends thereof |
-
2015
- 2015-07-03 CN CN201510387876.0A patent/CN104938515B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000051436A1 (en) * | 1999-03-05 | 2000-09-08 | Ecosmart Technologies, Inc. | Herbicidal compositions containing plant essential oils and mixtures or blends thereof |
Non-Patent Citations (2)
| Title |
|---|
| LUCIANA G. ANGELINI ET AL.: "Essential Oils from Mediterranean Lamiaceae as Weed Germination Inhibitors", 《J. AGRIC. FOOD CHEM.》 * |
| S.J.H.RLZVI ET AL.: "Geraniol, an allelochemical of possible use in integrated pest management", 《PLANT PROTECTION QUARTERLY》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106883047A (en) * | 2017-04-08 | 2017-06-23 | 叶长东 | Ratoon sugarcane cultivates fertilizer |
| CN106883047B (en) * | 2017-04-08 | 2021-04-27 | 叶长东 | Perennial root sugarcane cultivation fertilizer |
| CN116472818A (en) * | 2023-04-12 | 2023-07-25 | 石河子大学 | Method for promoting ragweed seed germination |
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| CN104938515B (en) | 2017-12-01 |
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