Prof.Dr. Metin DIĞRAK
Kahramanmaras Sutcu Imam Univ., Biology, Department Member
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Elazığ yöresinde yaygın olarak kullanılan pestisitlerin Bacillus Sp., Pseudomonas sp., karışık kültür ve toprak mikroorganizmaları ile parçalanma durumlarının incelenmesi / Degradation of pesticides used commonly in Elazığ province by Bacillus sp., Pseudomonas sp., soil microorganisms and in mixe...more
An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely... more
An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
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Kahramanmaras Sutcu Imam Universitesi Ziraat Fakultesi Biyosistem Muhendisligi Bolumu laboratuvarinda yurutulen calismada, Kahramanmaras bolgesinde sulamada yaygin olarak kullanilan 4 farkli firmaya ait damla sulama laterallerinin 7... more
Kahramanmaras Sutcu Imam Universitesi Ziraat Fakultesi Biyosistem Muhendisligi Bolumu laboratuvarinda yurutulen calismada, Kahramanmaras bolgesinde sulamada yaygin olarak kullanilan 4 farkli firmaya ait damla sulama laterallerinin 7 farkli basinc altinda (0.5, 0.75, 1.00, 1.25, 1.50, 1.75 ve 2.00 atm) gostermis olduklari damlatici akis ozellikleri, basinc-debi iliskisi, yapim farklilik katsayisi (CV), damla turdesligi (EU), sulama suyu es dagilim katsayisi (CU) ve istatistiksel uniformite (Us) degerlerinin uygunlugu Amerika Ziraat Muhendisligi Odasi (ASAE) standartlarina gore incelenmistir. Calisma sonucunda; damlaticilarin artan basinc degerlerinde debi degerlerinin de arttigi gorulmustur. Firmalarin 1 atmosfer basinc altinda vermis olduklari 4 lsa -1 ’lik debi degerlerinde A firmasinda % 1, B firmasinda % 15.5, C firmasinda % 1.5 ve D firmasinda % 12.5’luk bir farkliligin oldugu belirlenmistir. Ayrica, yapim farklilik katsayisi, damlatici turdesligi, turdeslik katsayisi ve istatis...
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In this study, we performed identification tests of twenty-seven Staphylococcus aureus strains isolated form throat cultures. Sensitivity test of those to novobiocin, amikacin, penisilin G, amoxici llin, ciprofloxacine, oxacillin,... more
In this study, we performed identification tests of twenty-seven Staphylococcus aureus strains isolated form throat cultures. Sensitivity test of those to novobiocin, amikacin, penisilin G, amoxici llin, ciprofloxacine, oxacillin, methicillin, tetracyclin e, cephalothin, nitrofurantoin, erythromycin, chlor amphenicol, cefoxitin and ampicillin antibiotics were investiga ted. It is found that all strains isolated from thr oat cultures are to susceptibil ciprofloxacin and
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The essential oil from the gum of Pistachio (Pistacia vera L. (Anacardiaceae)) grown in Turkey was obtained by the hydro-distillation method, and its chemical composition was analyzed by GC and GC-MS. Moreover, the antimicrobial... more
The essential oil from the gum of Pistachio (Pistacia vera L. (Anacardiaceae)) grown in Turkey was obtained by the hydro-distillation method, and its chemical composition was analyzed by GC and GC-MS. Moreover, the antimicrobial activities of the oil against the growth of 13 bacteria and 3 pathogenic yeasts were evaluated using the agar-disk diffusion and minimum inhibitory concentration (MIC) methods. The results showed that the essential oil contained about 89.67% monoterpenes, 8.1% oxygenated monoterpenes and 1.2% diterpenes. alpha-Pinene (75.6%), beta-pinene (9.5%), trans-verbenol (3.0%), camphene (1.4%), trans-pinocarveol (about 1.20%), and limonene (1.0%) were the major components. The antimicrobial results showed that the oil inhibited nine bacteria and all the yeasts studied, and the activities were considerably dependent upon concentration and its bioactive compounds such as carvacrol, camphene, and limonene. Moreover, the essential oil of the gum was found to be more effective yeastcide than Nystatin, synthetic yeastcide. Furthermore, the antibacterial activities of the oil were lower than those of standard antibiotics, ampicillin sodium, and streptomycine sulfate under the conditions studied.
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In this study, the antimicrobial activities of several parts of various trees grown in the Kahramanmaraş region of Turkey were investigated by the disc diffusion method. Chloroform, acetone and methanol extracts of leaves, resins, barks,... more
In this study, the antimicrobial activities of several parts of various trees grown in the Kahramanmaraş region of Turkey were investigated by the disc diffusion method. Chloroform, acetone and methanol extracts of leaves, resins, barks, cones and fruits of Pinus brutia Ten., Juniperus oxycedrus L., Abies cilicia Ant. & Kotschy Carr., Cedrus libani A. Rich. and Pinus nigra Arn. were prepared and tested against Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus cereus FMC 19, Escherichia coli DM, Klebsiella pneumoniae FMC 3, Enterobacter aerogenes CCM 2531, Staphylococcus aureus Cowan 1, Mycobacterium smegmatis RUT, Proteus vulgaris FMC 1, Listeria monocytogenes Scoot A, Pseudomonas aeruginosa DSM 5007, Candida albicans CCM 314, Candida tropicalis MDC 86 and Penicillium italicum K. The results showed that antifungal effects were not observed for the whole extracts, E. coli was not inhibited by any of the plant extracts except by the chloroform and acetone extracts of the leaves of A. cilicia, which showed inhibition zones of 16-18 mm, respectively. All the plant extracts used in this study inhibited the development of the other bacteria studied. When the results of this study were compared with an ampicillin standard, it was found that the microorganisms studied were generally susceptible, intermediate or resistant to the extracts of species when compared with the ampicillin standard. On the other hand, the acetone and methanol extracts of Juniperus fruits were found to be quite resistant.
Research Interests: Botany, Biology, Pharmacognosy, Fungi, Medicine, and 14 moreBiological Sciences, Phytotherapy, In Vitro, Antimicrobial activity, Bacteria, Trees, CHEMICAL SCIENCES, Anti-Bacterial Agents, Bacillus megaterium, Microbial Sensitivity Tests, Biological activity, Pinus Brutia, Medical and Health Sciences, and Antimicrobial agent
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In this study, it has been investigated that the sinergytic effect of some kind of juices with antibiotics. As a result of this study; juices’ and the antibiotics’ sinergytic effects ultimately has been given as positive results.... more
In this study, it has been investigated that the sinergytic effect of some kind of juices with antibiotics. As a result of this study; juices’ and the antibiotics’ sinergytic effects ultimately has been given as positive results. Interaction of the juices with five antibiotics, ampisilin (AMP), nitrofurantoin (F), azteonam (ATM), gentamycin (GM), amikacin (AK)- especially Gentamycin (GM) and Amikacin (AK) have been found as more effective on Pseudomonas aeruginosa, Enterobacter amnigenus, Enterecoccus faecium, Candida albicans and Saccharomyces cerevisiae.
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Dogada cok yaygin olarak bulunan Listeria turleri ve ozellikle de Listeria monocytogenes insan ve bircok hayvan turu icin patojen bir mikroorganizmadir. Son yillarda insanlarda gorulen Listeria salginlarinda basta sut ve sut urunleri... more
Dogada cok yaygin olarak bulunan Listeria turleri ve ozellikle de Listeria monocytogenes insan ve bircok hayvan turu icin patojen bir mikroorganizmadir. Son yillarda insanlarda gorulen Listeria salginlarinda basta sut ve sut urunleri olmak uzere et ve et urunleri, balik ve kanatli etleri gibi hayvansal kokenli gidalar uzerinde patojenik olarak onemli bir rol oynadigi ortaya konmustur. Bu calismada, Kahramanmaras ilinde cesitli satis yerlerinden temin edilen 30 tavuk eti ornegininde Listeria turleri arastirilmistir. Butun orneklere 30 °C’de zenginlestirme yontemi uygulanmis ve Listeria Selektif Agar besiyerine yapilan ekimlerin sonucu degerlendirilmistir. Đnceleme sonucunda orneklerin 20’sinde Listeria izole edilmistir. Yapilan biyokimyasal testler sonucunda bu izolatlarin tumunun Listeria grayi oldugu belirlenmistir.
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Bu calismada, Citrullus vulgaris Schrad. (Karpuz) atiklarindan tek hucre proteini uretimi ve uretimin optimizasyonu arastirilmistir. Karpuz suyuna aktive edilmis maya hurceleri (Candida utilis ve Saccharomyces cerevisiae) asilanarak Tek... more
Bu calismada, Citrullus vulgaris Schrad. (Karpuz) atiklarindan tek hucre proteini uretimi ve uretimin optimizasyonu arastirilmistir. Karpuz suyuna aktive edilmis maya hurceleri (Candida utilis ve Saccharomyces cerevisiae) asilanarak Tek Hucre Proteini (THP) uretilmistir. Karpuz suyu ilave edilen besin ortaminda farkli konsantrasyon, sicaklik, glukoz, fruktoz, sakkaroz ve pH’in etkisi arastirilarak en uygun THP uretim sartlari belirlenmistir. Arastirma sonucuna gore; S. cerevisiae % 30 karpuz suyu iceren ortamda, C. utilis ise %20 oraninda karpuz suyu iceren ortamda en iyi gelismeyi gostermistir. En uygun sicakligin C. utilis icin 30 oC oldugu belirlenirken, S. cerevisiae icin ise 25, 30 ve 35 oC’lerde benzer sonuclar alinmistir. Karbonhidratlarin THP uretimine etkisinin belirlenmesi icin yapilan calismalarin sonucunda S. cerevisiae icin %1 glukoz, C. utilis icin ise %2 glukoz iceren; S. cerevisiae icin %2 fruktoz, C. utilis icin %3 fruktoz; S. cerevisiae icin %3 sakaroz, C. utilis’i...
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The Parmelia furfuracea (L) Zopf. (Liken), Crocus chrysanthus (Herbert) Herbert (Cigdem), Rumex scutatus L., (Kuzukulagi), Myrtus communis L. subsps. communis Ic: Sibth. and Sm. (Mersin), Asphodelus aestivus L. (Ciris) Eugenia... more
The Parmelia furfuracea (L) Zopf. (Liken), Crocus chrysanthus (Herbert) Herbert (Cigdem), Rumex scutatus L., (Kuzukulagi), Myrtus communis L. subsps. communis Ic: Sibth. and Sm. (Mersin), Asphodelus aestivus L. (Ciris) Eugenia caryophyllata Thunb. (Karanfil) which were collected from Kahramanmaras and Hatay region have been investigated in respect of their antimicrobial and antifungal activities against different test organisms ( Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus, brevis FMC 3, Escherichia coli DM, Klebsiella pneumoniae FMC 5, Enterobacter aerogenes CCM 2531, Pseudomonas aeruginosa DSM 50071, Staphylococcus aureus COWAN 1, Listeria monocytogenes SCOTT A, bacteria and Candida albicans CCM 314 and Saccharomyces cerevisiae WET 136 by the Disc Diffusion Methods. The growth of the microorganisms which are used in this study have been inhibited by the extracts of Myrtus communis subsp. communis, Eugenia caryophyllata. However, the extract of Myrtus communis su...
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Abstract: In this study, the prevalence of Listeria spp. was investigated in the ice cream samples collected from the downtown stores of Kahramanmaras (28 samples) and Adana (30 samples). A total of 58 ice cream samples were analysed by... more
Abstract: In this study, the prevalence of Listeria spp. was investigated in the ice cream samples collected from the downtown stores of Kahramanmaras (28 samples) and Adana (30 samples). A total of 58 ice cream samples were analysed by the Food and Drug ...
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Humans find several uses for plants. Not only is it good for you from a nutritional standpoint, but it has also been used to cure a wide range of illnesses. In this research, we analysed Campanula strigosa Banks & Sol. for its antioxidant... more
Humans find several uses for plants. Not only is it good for you from a nutritional standpoint, but it has also been used to cure a wide range of illnesses. In this research, we analysed Campanula strigosa Banks & Sol. for its antioxidant and antibacterial properties. Total phenolic and flavonoid concentrations were also calculated. For this purpose, a soxhlet apparatus was used to extract ethanol and methanol from the plant's above-ground portions. The effectiveness of antioxidants was measured using Rel Assay kits. The agar dilution technique was used to test for antimicrobial activity against various bacterial and fungus species. Folin-Ciocalteu reagent was used to quantify total phenolic content. An aluminium chloride assay determined the total flavonoid concentration. Studies found that the plant extract had a TAS value of 4.974±0.259 mmol/L, a TOS value of 12.437±0.150 µmol/L, and an OSI value of 0.251±0.014. The plant extract was shown to be effective against bacterial st...
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Propolis, ari kovanlarinin dis ortamdan izole edilmesi, kuvvetlendirilmesi ve dezenfeksiyonu icin kullanilan dogal bir ari urunudur. Propolisin etanolik ekstraktinin insandaki tuberkuloza neden olan basiller dahil, Gram-Gram+basil ve... more
Propolis, ari kovanlarinin dis ortamdan izole edilmesi, kuvvetlendirilmesi ve dezenfeksiyonu icin kullanilan dogal bir ari urunudur. Propolisin etanolik ekstraktinin insandaki tuberkuloza neden olan basiller dahil, Gram-Gram+basil ve koklara karsi genel bir antimikrobiyal etkisinin oldugu bulunmustur. Propolis etanolik ekstraktinin farkli konsantrasyonlardaki cozeltileri, Escherichia coli ATTC 25922 disinda calismada kullandigimiz bakteri ve maya suslarinin gelismelerini engelledigi tespit edildi. Ekstrakte edilen yag asitlerinden nervonik asit (24:1) 15.8 mg/g, palmitik asit (16:0) 9.6 mg/g ve stearik asit (18:0) 2.72 mg/g propolis olarak belirlendi.
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Nowadays, technological development has ensured the welfare of human beings also, brought form hazardous problems to human health. One of these problems is pesticide residues that accumulate in the human bodies. Pesticides are used for... more
Nowadays, technological development has ensured the welfare of human beings also, brought form hazardous problems to human health. One of these problems is pesticide residues that accumulate in the human bodies. Pesticides are used for plant protection and therefore are taken in through the contaminated food. This problem is not considered locally; because of the international commerce and transportation it has become a problem of the whole world. in this investigation, degradation of pesticides (Chlorpyrifos-metil, Chloridazon, Diazinon, Ethion, Methidation and Primicarb) by Saccharomyces cerevisiae WET 136 was studied. Degradation of pesticides by Saccharomyces cerevisiae WET 136 was studied in Yeast Ekstract Dextrose Broth (YEGB) incubated at 25 °C for 5 days. Uninoculated medium served as controls. At the different period of incubation (l, 2, 3, 4 and 5 days) pesticides were extracted with ethyl acetate (Merck) and analysed in a Packart gas chroniaiograph model 439 using a Nitro...
Research Interests: Chemistry, Biology, Saccharomyces cerevisiae, Pesticide, Ethion, and 4 moreYeast, Ekoloji, Chlorpyrifos, and Diazinon
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An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely... more
An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
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The aim of our studies was to test the effect and role of vitamin E and selenium supplements on yeast cell. In this study, the effects of selenium (Se), vitamin E (Vit. E), and their combination (Se plus Vit. E) on the composition of... more
The aim of our studies was to test the effect and role of vitamin E and selenium supplements on yeast cell. In this study, the effects of selenium (Se), vitamin E (Vit. E), and their combination (Se plus Vit. E) on the composition of fatty acids and proteins were examined in Saccharomyces cerevisiae strains WET136 and 522. S. cerevisiae cells were grown up in YEPD medium supplemented with Se, Vit. E or their combination. It was found that the level of stearic acid was increased in all supplemented groups (p < 0.05; p < 0.001). The content of saturated and unsaturated fatty acids was decreased (p < 0.05; p < 0.01; p < 0.001) in Vit. E and Vit. E plus Se supplemented S. cerevisiae. On the other hand, Se alone caused an increase (p < 0.001) in the saturated fatty acids but a decrease (p < 0.05; p < 0.001) in the unsaturated fatty acids. Total proteins in S. cerevisiae were significantly increased (p < 0.001) by Vit. E supplement. There was no significant change observed in S. cerevisiae supplemented with Se. These findings indicate that membrane composition of S. cerevisiae is affected by both Vit. E and Se supplements.