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The comet assay is a useful technique for the study of genetic damage in humans exposed to environmental mutagens and carcinogens. In this study the effects of hydrogen peroxide (H(2)O(2)) and ultraviolet (UV) irradiation on 80 healthy... more
The comet assay is a useful technique for the study of genetic damage in humans exposed to environmental mutagens and carcinogens. In this study the effects of hydrogen peroxide (H(2)O(2)) and ultraviolet (UV) irradiation on 80 healthy individuals living in urban and rural areas with different smoking habits were investigated. Endonuclease III (endo III) treatment was also used to reveal the level of oxidized pyrimidine formation in these groups. The extent of damage and subsequent repair appear to be influenced by the living conditions (urban or rural areas). Smoking, however, was shown to have the most significant effect on DNA damage on all groups studied.
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The purpose of this study was to investigate children's preferences of soft drinks and their knowledge of the dental health problems caused by them. A sample from Larissa city in Central Greece was taken for this study, with a total... more
The purpose of this study was to investigate children's preferences of soft drinks and their knowledge of the dental health problems caused by them. A sample from Larissa city in Central Greece was taken for this study, with a total of 600 students (305 boys and 295 girls) aged 9-12 years old. Results showed that in all age groups milk is children's first choice of preference. Parents and friends influenced youngest and oldest children choices, while taste was also very important for older children. Children seemed not to believe in advertisements, although younger children realize that advertisements try to make them buy products. Lastly this study confirms our hypothesis, that children's knowledge of the dental health effects of soft drinks is limited. Resumen
The aim of this work was to investigate the relationship between mechanisms of DNA repair and apoptosis induced by oxidative stress (H2O2) in human lymphocytes. Using the comet assay, fluorescent microscopy, and DNA electrophoresis, we... more
The aim of this work was to investigate the relationship between mechanisms of DNA repair and apoptosis induced by oxidative stress (H2O2) in human lymphocytes. Using the comet assay, fluorescent microscopy, and DNA electrophoresis, we studied the DNA damage induced by hydrogen peroxide (H2O2) treatment, the time and the amount of repair of strand breaks, the type of cell death, and the influence of inhibitors of repair (nicotinamide). When lymphocytes were treated with H2O2, we observed an increased in necrosis compared to apoptosis. However, when nicotinamide (which inhibits DNA repair) was added, the mode of death reversed to increased apoptosis. These results indicate that nicotinamide "protects" resting lymphocytes exposed to H2O2 from necrosis but not from apoptosis.
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The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a... more
The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a delay of 36 h between heating and infection. The induction process requires protein synthesis only during the 3 h period immediately following heating; cycloheximide does not prevent the expression of enhanced reactivation if added to the cells after this time. Heat-enhanced reactivation exhibits properties similar in some respects to radiation-enhanced reactivation and indicates an increased capacity of the heated cells to tolerate DNA damage.
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Research Interests: Agriculture, Gamma Rays, DNA damage, Spain, Humans, and 5 morePesticides, Male, Adult, Occupational Exposure, and Lymphocytes
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... Permissions & Reprints. Comet assay for nuclear DNA damage. Stylianos M. Piperakis, Evangelos-E. Visvardis and Aspasia M. Tassiou. Available online 29 November 2003. Excerpt. Note: This is a one-page preview only. Click... more
... Permissions & Reprints. Comet assay for nuclear DNA damage. Stylianos M. Piperakis, Evangelos-E. Visvardis and Aspasia M. Tassiou. Available online 29 November 2003. Excerpt. Note: This is a one-page preview only. Click here to download preview. ...
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... GARAGOUNI-ARAIOU Fotini; Biology Unit, Department of Pre-School Education, University of Thessaly Argonofton and Filellinon. GIALAMA Xanthipi; Biology Unit, Department of Pre-School Education, University of Thessaly Argonofton and... more
... GARAGOUNI-ARAIOU Fotini; Biology Unit, Department of Pre-School Education, University of Thessaly Argonofton and Filellinon. GIALAMA Xanthipi; Biology Unit, Department of Pre-School Education, University of Thessaly Argonofton and Filellinon. 参考文献: 20件. ...
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The reversion frequency of an adenovirus 2 temperature-sensitive growth mutant irradiated with different doses of UV light was determined after infection of control, UV-irradiated and heat-shocked HeLa cells. No enhancement of mutagenesis... more
The reversion frequency of an adenovirus 2 temperature-sensitive growth mutant irradiated with different doses of UV light was determined after infection of control, UV-irradiated and heat-shocked HeLa cells. No enhancement of mutagenesis by treatment of the cells was observed. Heat-enhanced viral reactivation does not therefore display a significant error-prone component.
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Treatment of HeLa cells with low doses of the carcinogens aflatoxin B1, methyl methanesulfonate (MMS) or ethyl methanesulfonate (EMS) increases the survival rate of UV-irradiated adenovirus 2 (ade2). This increase is maximal if the time... more
Treatment of HeLa cells with low doses of the carcinogens aflatoxin B1, methyl methanesulfonate (MMS) or ethyl methanesulfonate (EMS) increases the survival rate of UV-irradiated adenovirus 2 (ade2). This increase is maximal if the time interval between cell treatment and virus infection is delayed by 36 h. No enhanced mutagenesis was found measuring the reversion frequency of a temperature-sensitive mutant of ade2 grown in HeLa cells treated with the same carcinogens. The enhanced viral reactivation observed does not, therefore, display a significant error-prone component.
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... Parkin et al., 1997). The effectiveness of a health and nutrition education programme (Manios et al., 2002), in changing certain chronic disease risk factors, was assessed after a 6-year intervention period. This school-based ...
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Research Interests: Program Evaluation, Health Promotion, Food Science, Nutrition, Science, and 13 moreDiet, Food and Nutrition, Children, Advertisement, Intervention, Cognitive processes, Food Preference, Science and Technology Education, Resistance to Change, Curriculum and Pedagogy, Emotional Response, Primary School Children, and Dietary Habits
A considerable number of radiation sensitive mutants of yeast (rad) have been isolated and have been shown to represent mutant alleles of approximately 30 loci whose gene products are involved in the repair of radiation damaged DNA.... more
A considerable number of radiation sensitive mutants of yeast (rad) have been isolated and have been shown to represent mutant alleles of approximately 30 loci whose gene products are involved in the repair of radiation damaged DNA. Although such mutants have been ...
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In the present study, the genotoxic effects of commonly applied pesticides were evaluated using the alkaline comet assay (pH > 13). The amount of DNA damage (% DNA in tail) in peripheral lymphocytes... more
In the present study, the genotoxic effects of commonly applied pesticides were evaluated using the alkaline comet assay (pH > 13). The amount of DNA damage (% DNA in tail) in peripheral lymphocytes of 49 male agricultural workers from Southern Poland were measured and compared to 50 men from the same area who had no previous occupational exposure to pesticides. No statistically significant differences in basal DNA damage were found between the study groups. In addition, exposure of peripheral blood lymphocytes to hydrogen peroxide (100 and 150 microM) or gamma-irradiation (2.5 or 4.2 Gy) led to a similar degree of additional DNA damage and subsequent repair (for 2 hr) for all studied populations. In conclusion, our results indicate that the greenhouse workers who participated in this study had no detectable increased DNA damage or alteration in their cellular response to DNA damage in comparison to our control population.
Research Interests: Agriculture, Gamma Rays, DNA damage, DNA repair, Biological Sciences, and 16 moreWorkplace, Environmental Sciences, Humans, Pesticides, Male, Hydrogen Peroxide, Comet Assay, Middle Aged, Questionnaires, Adult, Occupational Exposure, Time Factors, Lymphocytes, Environmental, Mutagens, and Case Control Studies
Obstructive sleep apnea (OSA) syndrome is a respiratory disease that is linked to heart attacks and high blood pressure. In the present study, we used the Comet assay to compare basal DNA damage and DNA damage induction by hydrogen... more
Obstructive sleep apnea (OSA) syndrome is a respiratory disease that is linked to heart attacks and high blood pressure. In the present study, we used the Comet assay to compare basal DNA damage and DNA damage induction by hydrogen peroxide, ethanol, and gamma-irradiation in lymphocytes from 35 OSA patients and 35 controls. We also measured the apoptosis and necrosis produced by these agents and the ability of the lymphocytes to repair the induced DNA damage. It was found that lymphocytes isolated from OSA patients had higher basal levels of DNA damage and were more sensitive to the effects of the DNA-damaging agents than lymphocytes from controls. OSA patients also had a reduced capacity to repair the DNA damage induced by the three agents, but apoptosis and necrosis were similar in OSA patients and the controls.
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The pesticides in use in Greek greenhouses include a number of agents known to be mutagens and carcinogens. In the present study, we evaluated whether occupational exposure of agricultural workers to a complex mixture of pesticides... more
The pesticides in use in Greek greenhouses include a number of agents known to be mutagens and carcinogens. In the present study, we evaluated whether occupational exposure of agricultural workers to a complex mixture of pesticides resulted in a significant increase in DNA damage in human peripheral blood lymphocytes (PBLs). A total of 116 healthy individuals were divided into groups based on exposure to pesticides, smoking status, and gender. Alkaline comet assays performed on PBLs from these individuals indicated no statistically significant differences in basal DNA damage between the study groups. In addition, exposure of PBLs to a dose of hydrogen peroxide led to a similar degree of DNA damage and subsequent repair for all the study populations. The results of the study indicate that the agricultural workers who participated in this study had no detectable increase in DNA damage or alteration in the cellular response to DNA damage.