Kaiser Jamil
Indian Institute of Chemical Technology, Genetics, Faculty Member
- Dr. Kaiser Jamil is a distinguished Scientist, Fellow of the "World Academy of Medical Sciences (FWAMS). Presently at Bhagwan Mahavir Medical Research Centre, Hyderabad. TS, India.edit
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Phylogeny reconstruction has attracted much attention from biologists and computer scientists from gene-order dataover the last few years. Ubiquitin is widespread in eukaryotes and plays a pivotal role in selective ATP dependent protein... more
Phylogeny reconstruction has attracted much attention from biologists and computer scientists from gene-order dataover the last few years. Ubiquitin is widespread in eukaryotes and plays a pivotal role in selective ATP dependent protein degradation in cells. It is also highly expressed in several types of cancers. Hence, in order to understand its diversification in eukarya we undertook this study to determine its phylogeny using various computational methods. All sequences of Ubiquitin conjugating enzyme were extracted from protein data bank (PDB), and using MaximumLikelihood method as implemented in PHYML and the unrooted phylogenetic tree was constructed. This tree had four major clusters and one mini cluster. We calculated the divergence for the clusters of the gene from the site-specific change of functional importance in protein sequence evolution. The divergence was then mapped on the 3-D structure of the ube2c protein obtained from the PDB Data bank: (IL7K) using RASMOL. Fro...
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Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was... more
Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl3 methods. The results have indicated the presence of a considerable amou...
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Epidemiological studies were conducted in pesticide-exposed agricultural workers along with an equal number of age-and sex-matched controls. All the 200 exposed volunteers were suffering from fever, nausea, headache and other abnormal... more
Epidemiological studies were conducted in pesticide-exposed agricultural workers along with an equal number of age-and sex-matched controls. All the 200 exposed volunteers were suffering from fever, nausea, headache and other abnormal symptoms and visited the hospital for ...
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Phosphatase and tensin homolog (PTEN) and p16INK4a (p16) genes are tumor suppressor genes, associated with epigenetic alterations. PTEN and p16 promoter hypermethylation is a major epigenetic silencing mechanism leading to cancer. The... more
Phosphatase and tensin homolog (PTEN) and p16INK4a (p16) genes are tumor suppressor genes, associated with epigenetic alterations. PTEN and p16 promoter hypermethylation is a major epigenetic silencing mechanism leading to cancer. The cooperation between PTEN and p16 in pathogenesis of cancers suggest that their combination might be considered as potential molecular marker for specific subgroups of patients. Hence, the present study aimed to investigate whether PTEN and p16 promoter methylations were involved in oral squamous cell carcinoma (OSCC) in south Indian subjects. DNA methylation quantitative analyses of the two candidate tumor suppressor genes PTEN and p16 were performed by methylation-specific polymerase chain reaction (MSP). Fifty OSCC biopsy samples and their corresponding non-malignant portions as controls were studied comparatively. The methylation status was correlated with the clinical manifestations. Twelve out of 50 patients (24 %) were found to be methylated for ...
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A study was initiated in the laboratory to compare the effects of spore dust and sawdust formulations of Bacillus thuringiensis var israeler~sis and B.sphaericus on immatures of laboratory and field samples of C.quinquefasciatus. Various... more
A study was initiated in the laboratory to compare the effects of spore dust and sawdust formulations of Bacillus thuringiensis var israeler~sis and B.sphaericus on immatures of laboratory and field samples of C.quinquefasciatus. Various types of ecological niches including cess pits, cement tanks and 'U' drain which form the habitats of the mosquitoes, were selected for this study. Bacilli formulations were tested against 2nd, 3rd and 4th instar larvae of laboratory and field collected samples as mentioned above and the LC50 values were determined by computer analyses. The LCsos of B.t/luringiensis were compared with those of B.sphaericus and it was concluded from this study that B.rhuringiensis is highly toxic to all stages of larvae in laboratory and field habitats. Both sawdust formulations produced 80-100% mortality after 24 hrs. in all stages of larvae from three habitats and in laboratory samples at 0.005% concentration. It was concluded from these results that B.rhur...
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Human Cytochrome P450 3A4 is a major P450 enzyme in the liver and gastrointestinal tract. It plays an important role in the metabolism of a wide variety of drugs, some endogenous steroids, and harmful environmental contaminants. To... more
Human Cytochrome P450 3A4 is a major P450 enzyme in the liver and gastrointestinal tract. It plays an important role in the metabolism of a wide variety of drugs, some endogenous steroids, and harmful environmental contaminants. To investigate the interindividual variation in CYP3A4 levels we have carried out a study on the genetic polymorphism of 100 breast cancer subjects using fine needle aspiration cytology (FNAC) sampling procedure. DNA was extracted from all the samples and PCR was carried out for detecting the CYP3A4 gene polymorphisms. We selected exon-7 and 10 which are present on the 5'-flanking coding region of the gene using the respective primers for PCR followed by direct automated sequencing method for detecting the mutations. These mutations were compared to the wild type sequence structures obtained from GenBank database (accession no. AF209389). We found two novel point mutations which are heterozygous mutant alleles. The two variant alleles were Ile222Arg and ...
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Excision wounds were made on the paravertebral area on the dorsal side of rat under light ether anaesthesia. The wound was a full thickness wound with removal of the skin upto the fascia. Animals were fed the drug naproxen in a gum acacia... more
Excision wounds were made on the paravertebral area on the dorsal side of rat under light ether anaesthesia. The wound was a full thickness wound with removal of the skin upto the fascia. Animals were fed the drug naproxen in a gum acacia suspension with low, median and high doses based on the LD50 value of the drug. The animals were dosed upto the time eschar separated and no raw wound was left. Healing was assessed by planimetric measurement of the wound, periodically, thereby following wound closure by contraction. Granulation tissue formation and wound strength was assessed by using the granuloma model. Epithelialization was also determined. The cellular activity was assessed histologically and the biochemical indices on day of wounding and after the complete healing of the wound. Granulation tissue was used in assessing tensile strength and contraction. Histological sections of the wound on different days were assessed for connective tissue formation by the Mallory-Heidenhan st...
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Studies on the toxicity of aerosol vaporizer formulation, allethrin were carried out on both male and female species of C. quinquefasciatus and M. domestica at different times of exposure. ANOVA models revealed significant differences in... more
Studies on the toxicity of aerosol vaporizer formulation, allethrin were carried out on both male and female species of C. quinquefasciatus and M. domestica at different times of exposure. ANOVA models revealed significant differences in the mean values of the percentage mortalities in relation to sex and time-of-exposure for both the species.
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Background: Probiotics are live microbial feed supplements, which positively affect the host animal by improving its intestinal microbial balance. Studies have shown probiotic activities of Lactobacillus isolated from dairy foods, which... more
Background: Probiotics are live microbial feed supplements, which positively affect the host animal by improving its intestinal microbial balance. Studies have shown probiotic activities of Lactobacillus isolated from dairy foods, which include the ability to inhibit the growth of other bacteria and the reduction of cholesterol. However, there is limited documented work on the probiotic activity. Natural foods are always accepted in an Indian context than any pharmaceutical preparation hence this study contributes to the repertoire of natural food for health, and can be formulated for consumption of humans. Hence, probiotic organisms are produced on a large scale and the cells are dried and employed as capsules that can reestablish the lost normal enteric flora, improving the microbial balance. Aim: A simple and reliable method for the identification of the beneficial strains has become important because of its increasing applications. This work was aimed at isolating and characteri...
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Vitiligo or leukoderma is a chronic skin condition that causes loss of pigment due to destruction of melanocytes, resulting in irregular pale patches of skin. Vitiligo is a polygenic disease and is associated with autoimmunity with an... more
Vitiligo or leukoderma is a chronic skin condition that causes loss of pigment due to destruction of melanocytes, resulting in irregular pale patches of skin. Vitiligo is a polygenic disease and is associated with autoimmunity with an unknown etiology. One of the candidate genes which has a strong association with several autoimmune diseases is CTLA-4 gene located in chromosome 2q33 region. We investigated the possible association between CTLA-4 gene polymorphism in exon 1 (A49G) and vitiligo in patients from South India and compared the distribution of this polymorphism to matched control groups. The polymorphism was detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method in 175 patients and 180 normal, age/ethnicity matched individuals. Consistency of genotype frequencies with the Hardy-Weinberg equilibrium was tested using a chi(2) test. There was no significant difference between the genotype (P = 0.93) and allele (P = 0.615) frequencies of CTLA-4 A49G polymorphism in patients and normal healthy individuals. However there was significant association of the CTLA-4 genotype (P = 0.02) and allelic frequency (P = 0.008) between the segmental and non-segmental sub groups within vitiligo. Our results indicate that there is no association between CTLA-4 A49G gene polymorphism and vitiligo in southern Indian population.
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Research Interests: Toxicology, Biology, DNA damage, DNA repair, Medicine, and 15 moreIndia, Insecticides, Genotoxicity, Animals, Single Cell, Comet Assay, Tilapia, Time Dependent, Time Factors, Food Sciences, Gel electrophoresis, Sensitivity and Specificity, Food and Chemical Toxicology, Tail Length, and Mutagenicity tests
... Hereditas 66: 21-30 Kaiser Jamil, Abjal Pasha Shaik, Mahboob M, D.Krishna (2004) Effect of Organophosphorus and Organochlorine pesticides (Monochrotophos, Chlorpyriphos, Dimethoate and Endosulfan) on Human lymphocytes in-vitto. ...
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Acute and Sub-acute toxic effects of a new pesticide phosphorothionate coded as RPR-V on testis of albino rat were studied. For the acute study, rats received a single dose of 30 mg/kg of RPR-V and sacrificed after 24 hours. For the... more
Acute and Sub-acute toxic effects of a new pesticide phosphorothionate coded as RPR-V on testis of albino rat were studied. For the acute study, rats received a single dose of 30 mg/kg of RPR-V and sacrificed after 24 hours. For the Subacute study, 1.42 mg/kg/day was administered orally to rats for 10 days and 21 days. Acute exposure of rats to RPR-V brought no change either in the Gonadosomatic Index (GSI) or in the structure of testis or in the serum levels of Testosterone. Similarly, no significant change was observed in the Glutathione S-transferase (GST) activity. But, in testis there was significant increased in the reduced Glutathione (GSH) and Acid Phosphatase (AcP), whereas Alkaline Phosphatase (AkP) levels decreased significantly at 24hr post treatment. On 7th day (withdrawal period) after the cessation of the treatment the GSH, AcP, and AkP levels reached to near control. The sub-acute study revealed a significant post treatment. Due to RPR-V treatment the testis AcP levels increased significantly at 21st day of medication but AkP levels decreased both at 10th and 21st day of post treatment. Histopathological studies showed that after 10th day testis showed considerable loss of spermatozoids and at 21st day complete derangement of cellular organization was observed. Testosterone levels decreased significantly after 10th day and remained significantly low at 21st day. However, withdrawal studies showed a recovery in testis of rat treated with RPR-V. GST, GSH, GSI, AcP and AkP values were recovered, testosterone levels were also recovered but recovery in testis structure remained at a low profile.
Research Interests: Endocrinology, Biology, Medicine, Insecticides, Internal Medicine, and 15 moreAnimals, Male, Glutathione, Alkaline phosphatase, Rats, Glutathione Transferase, Reproductive System, Acute Toxicity, Gonadosomatic Index, Science Health, ENVIRONMENTAL SCIENCE AND MANAGEMENT, Acid Phosphatase, Albino Rat, Reduced glutathione, and Male rat
Glutathione S-transferases (GSTs) are enzymes involved in the detoxification of several environmental mutagens, carcinogens and anticancer drugs. GST polymorphisms resulting in decreased enzymatic activity have been associated with... more
Glutathione S-transferases (GSTs) are enzymes involved in the detoxification of several environmental mutagens, carcinogens and anticancer drugs. GST polymorphisms resulting in decreased enzymatic activity have been associated with several types of solid tumors. We determined the frequencies of the deletion of two GST subfamilies genes, M1 and T1, in patients with acute lymphocytic leukemia ( ALL). Using polymerase chain reactions, we analyzed the GSTM1 and GSTT1 genotype in 135 patients with ALL (01-10 years) and 146 age matched controls. The prevalence of GSTM1 and GSTT1 homozygous resulting in null genotypes at the GSTM1 and GSTT1 loci were detected 60 (44.44%) and 48 (35.55%) patients, and 61 (41.75%) and 23 (15.75%) controls, respectively. The presence of at least one GST deletion proved to be an independent prognostic risk factor for response to treatment. In conclusion, individuals with GSTM1 or GSTT1 deletions (or deletions of both) may have an enhanced resistance to chemoth...
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Nosocomial infections are one of the occupational biohazards that affect the health of individuals with or without predisposing factors. Staphylococcus aureus is associated with significantly higher mortality and is associated with... more
Nosocomial infections are one of the occupational biohazards that affect the health of individuals with or without predisposing factors. Staphylococcus aureus is associated with significantly higher mortality and is associated with community-acquired serious nosocomial infections because strains generally show multiple drug resistance, which limits treatment possibilities. A total of 1800 patients in the state of Andhra Pradesh were screened for the presence of Staphylococcus species and were tested for antibiotic resistance. The results indicated that among ten antibiotics used in the present study, Amikacin and Azithromycin should be the drug of choice to treat S. aureus infection. It was observed that the resistance of most of the antibiotics tested showed increased resistance with increasing age. These results suggest that clinicians should consider age as an important factor while prescribing these antibiotics. Key words: Nosocomial, antibiotic resistance, Staphylococcus aureus.
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Introduction: Two common polymorphisms in cytochrome P450; family 1, subfamily A, polypeptide 1 (CYP1A1); have been implicated as a risk factor of prostate cancer, but individual studies have been inconclusive or controversial. We... more
Introduction: Two common polymorphisms in cytochrome P450; family 1, subfamily A, polypeptide 1 (CYP1A1); have been implicated as a risk factor of prostate cancer, but individual studies have been inconclusive or controversial. We reviewed studies on CYP1A1 polymorphisms in patients with prostate cancer. Materials and Methods: The strategy searching in the PubMed was based on combinations of prostate cancer, CYP1A1, CYP1A1 gene polymorphism, and genetic susceptibility. The last search update was May 2008. The retrieved articles and their bibliographies of were evaluated and reviewed independently by 2 experts. We shortlisted 19 studies, of which 14 on sporadic prostate cancer were analyzed. Overall, 2573 patients with prostate cancer and 2576 controls were analyzed. Results: The random effects odds ratio was 1.350 (95% confidence interval, 1.110 to 1.641; P = .003) for T/C polymorphism and 1.085 (95% confidence interval, 0.863 to 1.364; P = .49) for A/G polymorphism. The A/G polymor...
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Introduction : Breast cancer is the most common cancer among women worldwide. Sulfotransferase plays an important role in the formation of estrogen which is usually conferred as a risk factor for breast cancer. The SULT1A1*2 polymorphism... more
Introduction : Breast cancer is the most common cancer among women worldwide. Sulfotransferase plays an important role in the formation of estrogen which is usually conferred as a risk factor for breast cancer. The SULT1A1*2 polymorphism is likely to play an important role in the susceptibility to breast cancer. This polymorphism (G638A) in the sulfotransferase 1A1 (SULT1A1) gene may causes Arg213His amino acid change and consequently results in significantly reduced enzyme activity and thermostability. Materials and Methods : In this case-control study, we investigated the role of SULT1A1 G638A polymorphism in breast cancer patients. We genotyped 144 patients with breast cancer and 116 healthy control subjects, using a Polymerase chain reaction –Restriction Fragment length polymorphism (PCR-RFLP) method. Results : The frequencies of SULT1A1 G638G, G638A and A638A were 83.5%, 17.8%, and 1.4% in the breast cancer group and 89.5%, 4.0% and 0.0% in the control group. The results of our...
GST genotypes in head and neck cancer patients and its clinical implications
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Published by Madridge Publishers Abstract Background: Diabetic Nephropathy (DN) is one of the complications in patients with prolonged diabetes. Among the genetic risk factors for DN, TNF-α, an anti-inflammatory cytokine is proposed to... more
Published by Madridge Publishers Abstract Background: Diabetic Nephropathy (DN) is one of the complications in patients with prolonged diabetes. Among the genetic risk factors for DN, TNF-α, an anti-inflammatory cytokine is proposed to act in a paracrine/autocrine manner and is hypothesized to be associated with insulin resistance. In the current study, the relationship of the G ↆC variant of the TNF-α gene in patients, associated with other biochemical parameters with DN was investigated.
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The normal cell cycle process is a crucial process and is generally mediated by a number of regulatory genes. One of the most important regulators is the tumor suppressor p53, which in turn is regulated by MDM2 gene. The expression of p53... more
The normal cell cycle process is a crucial process and is generally mediated by a number of regulatory genes. One of the most important regulators is the tumor suppressor p53, which in turn is regulated by MDM2 gene. The expression of p53 and MDM2 is found to be frequently altered in many cancers and metastasis/ relapses .This is the first report to look at the evolutionary history of these genes to decipher the role of these genes in the tumorigenesis process using in silico methods. We also found that they showed high degree of sequence similarity across the mammalian species, indicating that these species probably share parallel cancer causing mechanisms. Their individual unrooted phylogenetic tree formed 5 clusters each; however, p53 gene was found in a large number of species whereas MDM2 was found in smaller number of species. The role of MDM2 is therefore limited and occurs in fewer species across the mammalian species. It is evident that these molecules play an important rol...
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Pharmacogenomics uses bioinformatics tools to study how an individual’s genetic makeup affects the body’s response to drugs. Our aim was towards the understanding of the possible role of structural variations in breast cancer drug... more
Pharmacogenomics uses bioinformatics tools to study how an individual’s genetic makeup affects the body’s response to drugs. Our aim was towards the understanding of the possible role of structural variations in breast cancer drug metabolising gene such as MTHFR that closely interact with the neoplastic drugs viz. Cyclophosphamide, 5-Fluorouracil, Methotrexate and others. We investigated the polymorphism in the gene that might affect the drug binding capacity of these molecules. We used VEGA genome browser for obtaining information about the gene–structure and dbSNPs of NCBI for SNPs. The Protein sequence was retrieved from NCBI database and using Swiss Homology method the protein structure was constructed. Linux based software TRITON was used to study and determine the mutations in the structure. For predicting the variations of drug binding capacities of the chemotherapeutic agents, we used Molegro Virtual Docker. This study revealed that the binding energy for mutated MTHFR struc...
Earlier studies have reported that Diabetes mellitus (DM) increases the complications of Severe acute respiratory syndrome coronavirus 2 (SARS CoV2) or Coronavirus disease 2019 (COVID19), and patients are more likely to experience severe... more
Earlier studies have reported that Diabetes mellitus (DM) increases the complications of Severe acute respiratory syndrome coronavirus 2 (SARS CoV2) or Coronavirus disease 2019 (COVID19), and patients are more likely to experience severe symptoms that could lead to mortality. One hypothesis states that hyperglycemic conditions facilitate the virus entry into the cell since the virus and the Angiotensin-Converting Enzyme 2 (ACE2) both require glucose for their function. However the exact interaction between the two has not been clearly defined from the cell biology point of view. The underlying mechanism for this differential effect in individuals with and without diabetes is not clearly understood. The reason for this could be due to the preexisting pathological pathways in patients with poorly controlled diabetes, which increases the risk of infectivity and are responsible for the higher levels of tissue injury and death in patients with diabetes [1]. However in this article we have focused mainly on COVID-19 induced diabetes as this area has lacuna. The diabetes specialists point out that many key metabolic tissues in the body, including pancreatic beta cells (b-cell) in the pancreas, adipose (fat storage) tissue, the small intestine, and kidneys, contain ACE2 receptors. They believe that when these viruses bind to ACE2 receptors, they may trigger changes in glucose metabolism that worsen preexisting diabetes or cause the condition to develop diabetes for the first time.
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The hypoglycemic effect of the aqueous extract ofAbroma augusta (Family: Steculiceae) was studied in normal as well as alloxan diabetic rats. Treatement of diabetic rats with 4 ml (4gm dry weight) of agueous extract ofA. augusta for 16... more
The hypoglycemic effect of the aqueous extract ofAbroma augusta (Family: Steculiceae) was studied in normal as well as alloxan diabetic rats. Treatement of diabetic rats with 4 ml (4gm dry weight) of agueous extract ofA. augusta for 16 weeks resulted in gradual but significant fall in fasting blood glucose and improvement in glucose tolerance. Serum total and LDL cholesterol and triacylglycerol which increased in diabetic rats showed improvement. These results show that the water extract ofA. augusta has both hypoglycemic and hypocholesterolemic effects.
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The present epidemiological study assessed smoking and non-smoking lung cancer patients with pesticide and other environmental exposures as additional risk factors. 152 confirmed lung cancer patients were screened and all patients were... more
The present epidemiological study assessed smoking and non-smoking lung cancer patients with pesticide and other environmental exposures as additional risk factors. 152 confirmed lung cancer patients were screened and all patients were divided into four different groups based on epidemiological findings, we found increase in lung cancer incidences in the category where smoking habit with exposure to pesticides was highest ((p =0.0006) followed by smoking habit and environmental carcinogens as risk factor. The incidence of lung cancer increased with age where probability was p<0.025 in the age group of 50-70 years. Pesticides and other carcinogenic exposures are added burdens in smokers as tobacco smoke contains several carcinogenic compounds and heavy metal contaminants such as cadmium, which are harmful to the lungs. This is the first report of our findings which recommends the inclusion of pesticide exposure in relation to/or in combination with smoking habits in epidemiologica...
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... Pte Microbial Transformation of Diterpenes: Hydroxylation of Sclareol, Manool and Derivatives by Mucor plumbeus Gerard Aranda*, Mohammed Samir El Kortbi ... 38 Amate, Y.; Bret6n, JL; GarciaGranados, A.; Martinez, A.; Onorato, ME ;... more
... Pte Microbial Transformation of Diterpenes: Hydroxylation of Sclareol, Manool and Derivatives by Mucor plumbeus Gerard Aranda*, Mohammed Samir El Kortbi ... 38 Amate, Y.; Bret6n, JL; GarciaGranados, A.; Martinez, A.; Onorato, ME ; Saenz de Buruaga, A. Tetrahedron 1990, 46 ...
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Thioredoxin reductase 1 (TXNRD1) and thioredoxin interacting protein (TXNIP) also known as thioredoxin binding protein 2 or vitamin D3-upregulated protein 1 are key players in oxidative stress control. Thioredoxin (TRX) is one of the... more
Thioredoxin reductase 1 (TXNRD1) and thioredoxin interacting protein (TXNIP) also known as thioredoxin binding protein 2 or vitamin D3-upregulated protein 1 are key players in oxidative stress control. Thioredoxin (TRX) is one of the major components of the thiol reducing system and plays multiple roles in cellular processes. Computational analyses of TXNRD1, TXNIP and TRX expressions have not been analyzed in relation to prognosis of breast cancer. High expression of TXNRD1 and low expression of TXNIP are associated with worst prognosis in breast cancer. Using bioinformatics applications we studied sequence analysis, molecular modeling, template and fold recognition, docking and scoring of thioredoxin as a target. The resultant model obtained was validated based on the templates from I-TASSER server and binding site residues were predicted. The predicted model was used for Threading and Fold recognition and was optimized using GROMACS. The generated model was validated using progra...
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Assessment of cytotoxicity and response to external factors like pesticides were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) or MTT assay, which measures mitochondrial metabolism in the entire cell... more
Assessment of cytotoxicity and response to external factors like pesticides were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) or MTT assay, which measures mitochondrial metabolism in the entire cell culture and provides information about the percentage of cell survival. Utilizing the MTT assay, the cytotoxicity of cypermethrin was determined on lymphocyte cultures from human peripheral blood samples, the short-term lymphocyte cultures were incubated with various aliquots of the cypermethrin and the LC50 was found to be 33.6 microM. Lymphocytes treated with low-doses (1/10 of LC50) of cypermethrin showed an increase in the frequency of chromosomal aberrations and found to be significant. Karyotype analysis revealed more satellite associations and chromosomal breaks in cypermethrin treated samples. Low-doses of the pesticide also induced single-strand breaks in the DNA as assessed by comet assay. The pesticide caused increase in the comet tail length...
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Anemia is one of the most important key factors affecting the quality of life (QOL) which needs addressing, as it is an important domain in optimal QOL. About 60-75% of the cancer patients invariably developed anemia most of them were at... more
Anemia is one of the most important key factors affecting the quality of life (QOL) which needs addressing, as it is an important domain in optimal QOL. About 60-75% of the cancer patients invariably developed anemia most of them were at advanced stages where treatment options were limited and mainly palliative. In advanced stages of cancer, QOL issues become an integral part of decision-making about various treatment options. Several key factors, including age, gender, co-morbidities, and quality of supportive care affects QOL in patients suffering from neoplasia. Fatigue appeared to exert adverse effect and impact on survival and QOL. Among the wide range of cancers anemia was most prevalent in hematological malignancies, followed by breast cancer, stomach, head and neck cancers. The probable causes that relate to various therapeutic protocols were dealt-with individually along with management strategies. In this study, we have evaluated severity of anemia for the first time in ca...
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A recent study reports that more than 99% of cases of cervical cancer worldwide contain HPV DNA. Hence, treatment options for cervical cancers are difficult due to multiplicity of the disease. Chemotherapy uses strong anti-cancer... more
A recent study reports that more than 99% of cases of cervical cancer worldwide contain HPV DNA. Hence, treatment options for cervical cancers are difficult due to multiplicity of the disease. Chemotherapy uses strong anti-cancer chemicals to kill cancer cells but to kill the viruses clinicians administer combination of drugs or higher doses of chemotherapy to control advanced cervical cancer and this practice causes severe side effects. Numerous cancer patients fail standard chemotherapy or develop resistance to chemotherapy during the course of treatment. Hence the aim of this investigation was to determine the chemo sensitivity of the five commonly used neoplastic drugs such as Geftinib, Cisplatin, 5-FU, Gemcitabine and Vinorelbine in vitro, and compare its toxicity on cervical cancer cells (HeLa) using lymphocytes (nucleated cells) as controls. Cytotoxicity in vitro was determined using the MTT assay; LC50 for all the drugs was calculated by regression equation. The morphologica...
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