Bacteria Penetrate the Inner Mucus Layer before Inflammation in the Dextran Sulfate Colitis Model
Figure 3
The epithelium can produce a normal mucus layer after 24 h of DSS treatment.
(A) The thickness of the inner firm mucus layer was measured in vivo in mice after removal of the outer loose mucus layer. The measurements were made in control animals (n = 6) and in mice treated with DSS for 24 h (n = 3). No significant difference was observed. (B) The loose and firm mucus from animals treated with DSS for 12, 18 and 24 h were reduced and separated by AgPAGE and stained with Alcian blue. The Muc2 bands migrate at their normal position for a monomer (M) and non-reducible oligomers.