Modulation of Macrophage Activation State Protects Tissue from Necrosis during Critical Limb Ischemia in Thrombospondin-1-Deficient Mice
Figure 3
Histological analysis showing tissue protection in tsp-1−/− mice.
(A&B) H&E staining of gastrocnemius muscle sections at d4 were performed in tsp-1−/− (A) and wt (B) mice (scale bar = 500 µm). Right panel show higher magnifications of preserved area (C), infiltrated area in tsp-1−/− (E) and in wt (G) and necrotic non-infiltrated area (I), with adjacent slides immunostained for macrophages using Mac-3 Ab (D, F, H, J, respectively); scale bar = 100 µm. (K) Quantification of histologically different area types surface (see text for definition); mean±S.E.M. are shown, * = p<0.05, n = 9. (L) Quantification of regenerating basic myofibers in necrotic areas, mean±S.E.M. are shown, ** = p<0.01, n = 9.