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Sample GSM312007 Query DataSets for GSM312007
Status Public on Jul 25, 2009
Title Left atria Ntg 1657
Sample type RNA
 
Source name RNA from mouse heart atria, Ntg
Organism Mus musculus
Characteristics male C57BL/6/FVB/N
Treatment protocol Transgenics models dnPI3k, Mst1 and dnPI3K-Mst1 were made accordingly.
Growth protocol The mice were on a standard chow diet, and were kept in temperature controlled cages (21 - 23 degree celcius) and maintained a 12 hour light/dark cycle
Extracted molecule total RNA
Extraction protocol Heart was homogenized individually using motar and pestle in liquid nitrogen. Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen).
Label biotin
Label protocol Fragmented and biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA for Affymetrix Mouse Gene 1.0 ST arrays
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were processed through second-cycle single stranded cDNA, fragmented and labelled cDNA were then hybridized for 16 hr at 45C on Affymetrix Mouse Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Gene arrays were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from heart atria
Data processing Gene array data is processd by Expression Console software in which mean probeset signals for all probesets are analysed. A receiver operating curve (ROC) is generated by thrseholding the computed value for the probesets. PLIER signals that pass this QC control is used for subsequent analysis.
 
Submission date Aug 12, 2008
Last update date Jul 24, 2009
Contact name Ruby CY Lin
E-mail(s) rubyl@unsw.edu.au, ruby.lin@sydney.edu.au
Organization name The Westmead Institute for Medical Research
Street address 176 Hawkesbury Road, Westmead
City Sydney
State/province NSW
ZIP/Postal code 2145
Country Australia
 
Platform ID GPL6246
Series (1)
GSE12420 Gene profiling of heart atria in PI3K and Mst1 mouse models

Data table header descriptions
ID_REF
VALUE Expression Console signal intensity (in Log2 space).

Data table
ID_REF VALUE
10338001 11.355999946594
10338003 10.003499984741
10338004 8.9947004318237
10338017 12.408699989319
10338025 8.7484903335571
10338026 12.82439994812
10338029 9.3042097091675
10338035 8.6281900405884
10338036 9.472149848938
10338037 4.7409000396729
10338041 10.446999549866
10338042 9.9344997406006
10338044 11.521599769592
10338047 8.0870800018311
10338056 2.8543899059296
10338059 12.73390007019
10338060 5.4967098236084
10338063 3.7437400817871
10338064 7.4482097625732
10338065 8.1711101531982

Total number of rows: 35512

Table truncated, full table size 862 Kbytes.




Supplementary file Size Download File type/resource
GSM312007.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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