GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) products are the ideal tools for expressing or modifying genes in a broad range of cell and tissue types, especially in vivo, with high efficiency and enhanced safety. GeneCopoeia’s optimized helper-free human AAV system allows viral packaging without potentially pathogenic helper adenovirus. Many pre-made AAV are available in 16 different serotypes, such as fluorescent reporters. You can also request us to produce custom AAV for genes up to 3 kb in length.
High titers. Titer of purified AAV particles can be up to 1014 GC/ml.
High adaptability. Virtually all serotypes are available.
Versatile. Usable in a broad range of host cell and tissue types.
Low toxicity. Does not integrate into the host genome.
Low immunogenicity. Minimal host immune response.
Safe. Not associated with any human disease
Search all AAV products by gene ID or accession number
AAVPrime™ uses a helper virus-free system for the safe preparation of high titer AAV. HEK293T cells are co-transfected with three plasmids encoding the factors necessary for recombinant AAV packaging. The AAV particles are then either membrane purified or purified by two-phase partitioning for high-titer AAV ready for in vivo animal use.
The destination vector carries your gene of interest (GOI) and two ITRs. You can choose from a variety of destination vectors carrying promoters including CMV, EF1a, CAG, CBh and other tissue-specific promoters, and reporter gene GFP for optimal gene expression and detection.(Learn more about our vector collection); the plasmid that carries the Rep and Cap genes from wild type AAV and the plasmid that carries adenovirus VA, E4 and E2A genes required for efficient AAV production are co-transfected with the destination vector for AAV packaging.
Figure 1. AAV of different serotypes carrying GFP were used to transduce the H1299 cell line as shown in the figure above. Fluorescent images (GFP) show that cells are transduced with AAV-GFP.
Figure 2. MyoAAV and AAV-MG indicated its tissue specificity in vitro infection. AAV-9, MyoAAV and AAV-MG carrying GFP were used to transduce the Rhabdomyosarcoma (RD) cell line(left) or Human microglia cell line (HmC3)(right).
AAV Serotype Choices
GeneCopoeia provides AAV for all the commonly-occurring AAV serotypes, including AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV-DJ, AAV-DJ/8, MyoAAV, AAV-MG, AAV-PHP.eB and AAV-BI30. The intensity of gene expression and tissue tropism depends highly on the AAV serotype used in different tissues.
Different serotypes differ mainly in surface protein (capsid). Carefully choosing the serotype enables robust gene expression in specific tissues with minimal immune response. Refer to the table below to compare the essential differences between different serotypes.
AAV Serotypes Available
Serotype
Primary Target Tissues
Description
AAV-1
Muscle
Best for cardiac muscle, skeletal muscle, neuronal and glial tissue.
AAV-2
Muscle, Liver, Retina
Oldest and most commonly-used serotype. Best for neurons, muscle, liver, and brain.
AAV-3
Megakaryocytes
Best for megakaryocytes, muscle, liver, lung, and retina.
AAV-4
Retina
Best for neurons, muscle, brain, and retina.
AAV-5
Lung
Best for lung, neurons, synovial joint, retina, and pancreas.
AAV-6
Muscle, Lung
Best for lung, liver, and heart.
AAV-7
Muscle, Retina, Neurons
Best for muscle, neurons, and liver.
AAV-8
Liver
Best for muscle, brain, liver, and retina.
AAV-9
Various
Best for muscle, heart, liver, lung, and brain.
AAV-10
Pleura, CNS
Cloned from Cynomolgus, almost identical with AAVrh10 except for 12 amino acids in VP1. Best for lung, muscle, heart, CNS, and liver.
AAV-DJ
Various
A mixture of 8 naturally-occurring serotypes. Efficiently transduces a wide variety of cell types in vitro.
AAV-DJ/8
Various
A variant of AAV-DJ with a mutation that permits infection of liver as well as other tissues in vivo.
MyoAAV NEW!
Muscle tissue
Best specificity for muscle tissue.
AAV-MG NEW!
Microglia
Best specificity for microglia in the brain.
AAV-PHP.eB NEW!
CNS
Cross the blood–brain barrier (BBB) and has enhanced CNS tropism.
AAV-BI30 NEW!
CNS
Specifically and efficiently transduces endothelial cells throughout the CNS.
We offer AAV of virtually all serotypes with titers starting from 5×1012 GC/ml packaged with ORF cDNA, CRISPR/Cas9 or shRNA. Click the links below to search for your gene(s) of interest.
For custom AAV packaging service, please contact us at inquiry@genecopoeia.comto get a quote.
Choose from our collection of AAV vectors with different promoters and fluorescent markers for your gene expression. The AAV can be packaged with ORF cDNA clones up to 3 kb in length for long-term expression.
Transduction-ready AAV delivering shRNA of varying lengths (19 to 29 bases) that were designed using a proprietary algorithm to make shRNA expression constructs that have high knockdown efficiency with minimal off-target effect.
By default, all options for AAV serotype, gene of interest, and volume are listed in the table below. Use the drop-down menus to narrow down the number of options displayed.
The destination vector carries your gene of interest (GOI) and two ITRs. You can choose from a variety of destination vectors carrying promoters including CMV, EF1a, CAG, CBh and other tissue-specific promoters, and reporter gene GFP for optimal gene expression and detection. (Learn more about our vector collection); the plasmid that carries the Rep and Cap genes from wild type AAV and the plasmid that carries adenovirus VA, E4 and E2A genes required for efficient AAV production are co-transfected with the destination vector for AAV packaging.