Academia.edu no longer supports Internet Explorer.
To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade your browser.
Tissue Engineering
Cryopreservation of Collagen-Based Tissue Equivalents. II. Improved Freezing in the Presence of Cryoprotective Agents2004 •
2003 •
2011 •
Cold Spring Harbor Protocols
Cryosectioning Tissues2008 •
INTRODUCTIONCryosections are rapidly and relatively easily prepared prior to fixation, and they provide a good system for visualizing fine details of the cell. Although cryosections are physically less stable than paraffin- or resin-embedded sections, they are generally superior for the preservation of antigenicity and therefore the detection of antigens by microscopy. The preparation of cryosections does not involve the dehydration steps typical of other sectioning methods, and, furthermore, sectioning, labeling, and observation of specimens can usually be carried out in one day. In general, the sample is frozen quickly in either isopentane or liquid nitrogen. (Small samples such as cells and small tissues may be mixed in a slurry of an inert support medium such as optimal cutting temperature [OCT] compound before freezing). Rapid freezing reduces ice crystal formation and minimizes morphological damage. Frozen sections may be used for a variety of procedures, including immunochemi...
Cell Transplantation
Cryopreservation: An Overview of Principles and Cell-Specific Considerations2021 •
The origins of low-temperature tissue storage research date back to the late 1800s. Over half a century later, osmotic stress was revealed to be a main contributor to cell death during cryopreservation. Consequently, the addition of cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol (GLY), ethylene glycol (EG), or propylene glycol (PG), although toxic to cells at high concentrations, was identified as a necessary step to protect against rampant cell death during cryopreservation. In addition to osmotic stress, cooling and thawing rates were also shown to have significant influence on cell survival during low temperature storage. In general, successful low-temperature cell preservation consists of the addition of a CPA (commonly 10% DMSO), alone or in combination with additional permeating or non-permeating agents, cooling rates of approximately 1ºC/min, and storage in either liquid or vapor phase nitrogen. In addition to general considerations, cell-specific re...
Scanning microscopy
Rapid freezing techniques and cryoprotection of biomedical specimens1987 •
It has been realized almost from the start of biological electron microscopy that the use of low temperature in specimen preparation might alleviate the problems associated with conventional chemical fixation and plastic embedding. In practice it has been very difficult to realize any benefits, owing to the problems encountered in trying to prepare samples with the water transformed to ice, yet without the sample itself hopelessly distorted by ice crystals. The difficulty is caused by the poor diffusibility of heat through water, the main constituent of almost all biological samples. Even with a maximal temperature gradient between the sample surface and the coolant, and a perfect contact between the two, heat diffusibility through the sample quickly becomes the limiting parameter affecting cooling rate. Unless the cooling rate is as high as 10(2) or even 10(3) degrees C.msec.-1, the ice formed will be crystalline. Such high cooling rates can only be attained in the first few microm...
Annals of Biomedical Engineering
Thermal Injury Prediction During Cryoplasty Through In Vitro Characterization of Smooth Muscle Cell Biophysics and Viability2008 •
BioMed Research International
Methods of Cryoprotectant Preservation: Allogeneic Cellular Bone Grafts and Potential EffectsDebridement of the bone surface during a surgical fusion procedure initiates an injury response promoting a healing cascade of molecular mediators released over time. Autologous grafts offer natural scaffolding to fill the bone void and to provide local bone cells. Commercial bone grafting products such as allografts, synthetic bone mineral products, etc., are used to supplement or to replace autologous grafts by supporting osteoinductivity, osteoconductivity, and osteogenesis at the surgical site. To assure osteogenic potential, preservation of allogeneic cells with cryoprotectants has been developed to allow for long-term storage and thus delivery of viable bone cells to the surgical site. Dimethyl sulfoxide (DMSO) is an intracellular cryoprotectant commonly used because it provides good viability of the cells post-thaw. However, there is known cytotoxicity reported for DMSO when cells are stored above cryogenic temperatures. For most cellular bone graft products, the cryoprotecta...
El sitio de las cosas: la Alta Edad Media en contexto, 2020, ISBN 978-84-9717-707-8, págs. 67-82
Los últimos edificios domésticos, de servicio portuario y productivos del suburbio de "Tarracona" (s. VII-VIII): un ensayo holístico2020 •
International Conferencre on Simulation of Semiconductor Processes and Devices
A new gate current model accounting for a non-Maxwellian electron energy distribution functionBulletin of Volcanology
Introduction to the Special Issue of Bulletin of Volcanology, “The Cerro Galan Ignimbrite and Caldera: characteristics and origins of a very large volume ignimbrite and its magma system”2011 •
HAL (Le Centre pour la Communication Scientifique Directe)
La cartographie de l'aléa et du risque rocheux sur le territoire de la Côte Bleue (13) : développement d'une méthodologie intégrant les contraintes et enjeux du gestionnaire2022 •
2011 •
Journal of Animal Science
Assessment of the vasoactive effects of the (S)-epimers of ergot alkaloids in vitro2020 •
Czechoslovak Journal of Physics
On the Unitarity of D = 9, 10, 11 Conformal Supersymmetry2004 •