human CD45 antibody, Cy5 conjugate

Contents: Cy5 anti-human CD45 (LCA)
Catalog Number: 19-0459
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI30
Isotype: Mouse IgG1, κ
HLDA No.: IV N816

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19-0459-71

25 tests

19-0459-73

100 tests

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0459	Affinity Purified anti-human CD45 (LCA)	N/A	N/A	FC IHC
13-0459	Biotin anti-human CD45 (LCA)	N/A	N/A	FC
11-0459	FITC anti-human CD45 (LCA)	488	518	FC
12-0459	PE anti-human CD45 (LCA)	488	575	FC
15-0459	PE-Cy5 anti-human CD45 (LCA)	488	670	FC
17-0459	Allophycocyanin (APC) anti-human CD45 (LCA)	633	660	FC
19-0459	Cy5 anti-human CD45 (LCA)	633	670	FC
25-0459	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45 (LCA)	488	760	FC
35-0459	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD45 (LCA)	488	690	FC
57-0459	Pacific Blue® anti-human CD45 (LCA)	402	421	FC
27-0459	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD45 (LCA) (APC-Cy7 Equivalent)	633	779	FC
45-0459	PerCP-Cy5.5 anti-human CD45 (LCA) (PE-Cy5.5 replacement)	488	690	FC
93-0459	eFluor^TM 605^NC anti-human CD45 (LCA)	350-500	605	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI30 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
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