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eFluor products are not offered for sale or use in certain regions. Please contact an eBioscience representative for more information on whether our products are available for purchase in your area. Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications ReportedFor research use only, not for diagnostic or therapeutic use. This HI30 antibody has been reported for use in flow cytometric analysis. Applications TestedThis HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using the eFluorTM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl/test (one million cells in a 100μL total staining volume). The isotype control eFluorTM 605NC mouse IgG1 (cat. 93-4714) should be used at 5μL/test. Laser/Filter Recommendation: When using eFluorTM 605NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information. Buffer Recommendation: Comparison of eFluorTM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluorTM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here. Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required. Click here to view our list of eFluorTM Nanocrystal Frequently Asked Questions. References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. Related ProductsCat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer Cat. 93-4714 eFluor™ 605NC Mouse IgG1, Isotype Control |
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