Proteases or proteolytic enzymes form one of the largest and more important groups of
enzymes. Proteases selectively catalyze the hydrolysis of peptide bonds and can be divided …

Proteolytic enzymes are key signaling molecules in both normal physiological processes
and various diseases. After synthesis, protease activity is tightly controlled. Consequently …

Protein analysis under biological conditions is now regarded as indispensable for
understanding the structure and function of proteins, in addition to in vitro studies using …

The modification of proteins with synthetic probes is a powerful means of elucidating and
engineering the functions of proteins both in vitro and in live cells or in vivo. Herein we …

Activity‐based protein profiling (ABPP) has matured into a standard method for the fast,
sensitive, and selective identification of enzyme activity and inhibitors in proteomes. By …

Diaryl esters of α-aminophosphonates are a group of low molecular weight inhibitors of
serine proteases. For over 30 years these molecules have captured the attention of …

Proteases are important targets for the treatment of human disease. Several protease
inhibitors have failed in clinical trials due to a lack of in vivo specificity, indicating the need …

Cathepsin K (CatK) is a lysosomal cysteine protease whose highest expression is found in
osteoclasts, which are the cells responsible for bone resorption. Investigations of the …

The activity of proteases is tightly regulated, and dysregulation is linked to a variety of
human diseases. For this reason, ABPP is a well-suited method to study protease biology …

Activity-based protein profiling enables the specific detection of the active fraction of an
enzyme and is of particular use for the profiling of proteases. The technique relies on a …