Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection
D Tokuhara, B Álvarez, M Mejima… - The Journal of …, 2013 - Am Soc Clin Investig
D Tokuhara, B Álvarez, M Mejima, T Hiroiwa, Y Takahashi, S Kurokawa, M Kuroda…
The Journal of clinical investigation, 2013•Am Soc Clin InvestigRotavirus-induced diarrhea is a life-threatening disease in immunocompromised individuals
and in children in developing countries. We have developed a system for prophylaxis and
therapy against rotavirus disease using transgenic rice expressing the neutralizing variable
domain of a rotavirus-specific llama heavy-chain antibody fragment (MucoRice-ARP1).
MucoRice-ARP1 was produced at high levels in rice seeds using an overexpression system
and RNAi technology to suppress the production of major rice endogenous storage proteins …
and in children in developing countries. We have developed a system for prophylaxis and
therapy against rotavirus disease using transgenic rice expressing the neutralizing variable
domain of a rotavirus-specific llama heavy-chain antibody fragment (MucoRice-ARP1).
MucoRice-ARP1 was produced at high levels in rice seeds using an overexpression system
and RNAi technology to suppress the production of major rice endogenous storage proteins …
Rotavirus-induced diarrhea is a life-threatening disease in immunocompromised individuals and in children in developing countries. We have developed a system for prophylaxis and therapy against rotavirus disease using transgenic rice expressing the neutralizing variable domain of a rotavirus-specific llama heavy-chain antibody fragment (MucoRice-ARP1). MucoRice-ARP1 was produced at high levels in rice seeds using an overexpression system and RNAi technology to suppress the production of major rice endogenous storage proteins. Orally administered MucoRice-ARP1 markedly decreased the viral load in immunocompetent and immunodeficient mice. The antibody retained in vitro neutralizing activity after long-term storage (>1 yr) and boiling and conferred protection in mice even after heat treatment at 94°C for 30 minutes. High-yield, water-soluble, and purification-free MucoRice-ARP1 thus forms the basis for orally administered prophylaxis and therapy against rotavirus infections.