Production and evaluation of a monoclonal antibody to Clostridium perfringens type D epsilon toxin.
MI El-Enbaawy, YA Abdalla, AZ Hussein… - The Egyptian Journal …, 2003 - europepmc.org
MI El-Enbaawy, YA Abdalla, AZ Hussein, RM Osman, SA Selim
The Egyptian Journal of Immunology, 2003•europepmc.orgA monoclonal antibody (mAb) against C. perfringens type D epsilon toxin was produced in
hybridoma tissue culture supernatant and in BALB/C mice ascitic fluid. The mAb reacted with
single band of the purified epsilon toxin and efficiently neutralized the lethal effect of epsilon
toxin in mice. A competitive ELISA (C-ELISA) was developed using this mAb and used to
evaluate C. perfringens type D vaccines. Individual serum samples from vaccinated rabbits
were tested using C-ELISA and mouse neutralization test (MNT). The mAb based C-ELISA …
hybridoma tissue culture supernatant and in BALB/C mice ascitic fluid. The mAb reacted with
single band of the purified epsilon toxin and efficiently neutralized the lethal effect of epsilon
toxin in mice. A competitive ELISA (C-ELISA) was developed using this mAb and used to
evaluate C. perfringens type D vaccines. Individual serum samples from vaccinated rabbits
were tested using C-ELISA and mouse neutralization test (MNT). The mAb based C-ELISA …
A monoclonal antibody (mAb) against C. perfringens type D epsilon toxin was produced in hybridoma tissue culture supernatant and in BALB/C mice ascitic fluid. The mAb reacted with single band of the purified epsilon toxin and efficiently neutralized the lethal effect of epsilon toxin in mice. A competitive ELISA (C-ELISA) was developed using this mAb and used to evaluate C. perfringens type D vaccines. Individual serum samples from vaccinated rabbits were tested using C-ELISA and mouse neutralization test (MNT). The mAb based C-ELISA correlated with the conventional MNT for estimation of antitoxin level.
europepmc.org