Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor
N Raven, S Schillberg, S Rasche - Recombinant Proteins from Plants …, 2016 - Springer
N Raven, S Schillberg, S Rasche
Recombinant Proteins from Plants: Methods and Protocols, 2016•SpringerTobacco BY-2 cells are an attractive platform for the manufacture of a variety of
biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up
cultivation of human IgG–secreting BY-2 cells in a 200 L orbitally shaken disposable
bioreactor, resulting in cell growth and recombinant protein yields that are proportionately
comparable with those obtained from cultivations in 500 mL shake flasks. Furthermore, we
present an efficient downstream process for antibody recovery from the viscous spent culture …
biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up
cultivation of human IgG–secreting BY-2 cells in a 200 L orbitally shaken disposable
bioreactor, resulting in cell growth and recombinant protein yields that are proportionately
comparable with those obtained from cultivations in 500 mL shake flasks. Furthermore, we
present an efficient downstream process for antibody recovery from the viscous spent culture …
Abstract
Tobacco BY-2 cells are an attractive platform for the manufacture of a variety of biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up cultivation of human IgG–secreting BY-2 cells in a 200 L orbitally shaken disposable bioreactor, resulting in cell growth and recombinant protein yields that are proportionately comparable with those obtained from cultivations in 500 mL shake flasks. Furthermore, we present an efficient downstream process for antibody recovery from the viscous spent culture medium using expanded bed adsorption (EBA) chromatography.
Springer