Spontaneous pepsin C-catalyzed activation of human pepsinogen C in transgenic rice cell suspension culture: Production and characterization of human pepsin C
A human pepsinogen C (hPGC) gene was synthesized with rice-optimized codon usage and
cloned into a rice expression vector containing the promoter, signal peptide, and terminator
derived from the rice α-amylase 3D (Ramy3D) gene. In addition, a 6-His tag was added to
the 3′ end of the synthetic hPGC gene for easy purification. The plant expression vector
was introduced into rice calli (Oryza sativa L. cv. Dongjin) mediated by Agrobacterium
tumefaciens. The integration of the hPGC gene into the chromosome of the transgenic rice …
cloned into a rice expression vector containing the promoter, signal peptide, and terminator
derived from the rice α-amylase 3D (Ramy3D) gene. In addition, a 6-His tag was added to
the 3′ end of the synthetic hPGC gene for easy purification. The plant expression vector
was introduced into rice calli (Oryza sativa L. cv. Dongjin) mediated by Agrobacterium
tumefaciens. The integration of the hPGC gene into the chromosome of the transgenic rice …