Enhancement of recombinant human serum albumin in transgenic rice cell culture system by cultivation strategy
YK Liu, YT Li, CF Lu, LF Huang - New biotechnology, 2015 - Elsevier
Highlights•The yield of rHSA was increased sixfold by this one-step approach.•The airlift
bioreactor is better than the stirred tank bioreactor for this expression system.•rHSA can be
enriched in MS medium by using an airlift-type bioreactor.Fusion of the sugar-starvation-
induced αAmy3 promoter with its signal peptide has enabled secretion of recombinant
human serum albumin (rHSA) into the culture medium. To simplify the production process
and increase the rHSA yield in rice suspension cells, a one-step strategem without medium …
bioreactor is better than the stirred tank bioreactor for this expression system.•rHSA can be
enriched in MS medium by using an airlift-type bioreactor.Fusion of the sugar-starvation-
induced αAmy3 promoter with its signal peptide has enabled secretion of recombinant
human serum albumin (rHSA) into the culture medium. To simplify the production process
and increase the rHSA yield in rice suspension cells, a one-step strategem without medium …