Clostridium difficile binary toxin (CDT) and diarrhea
RJ Carman, AL Stevens, MW Lyerly, MF Hiltonsmith… - Anaerobe, 2011 - Elsevier
Clostridium difficile is a major enteropathogen of humans. It produces two main virulence
factors, toxins A and B. A third, less well known toxin, C. difficile toxin (CDT), is a binary toxin
composed of distinct enzymatic (CdtA) and cell binding/translocation (CdtB) proteins. We
used a novel enzyme linked immunoassay (EIA) to detect CdtB protein in feces and culture
fluids. Additionally, PCR was used to assay C. difficile isolates from fecal samples for the
CDT locus (CdtLoc). Although the results from 80 isolates suggest no relationship between …
factors, toxins A and B. A third, less well known toxin, C. difficile toxin (CDT), is a binary toxin
composed of distinct enzymatic (CdtA) and cell binding/translocation (CdtB) proteins. We
used a novel enzyme linked immunoassay (EIA) to detect CdtB protein in feces and culture
fluids. Additionally, PCR was used to assay C. difficile isolates from fecal samples for the
CDT locus (CdtLoc). Although the results from 80 isolates suggest no relationship between …