Cleavage of a C-terminal peptide is essential for heptamerization of Clostridium perfringens ε-toxin in the synaptosomal membrane
S Miyata, O Matsushita, J Minami, S Katayama… - Journal of Biological …, 2001 - ASBMB
Activation of Clostridium perfringens ε-protoxin by tryptic digestion is accompanied by
removal of the 13 N-terminal and 22 C-terminal amino acid residues. In this study, we
examined the toxicity of four constructs: an ε-protoxin derivative (PD), in which a factor Xa
cleavage site was generated at the C-terminal trypsin-sensitive site; PD without the 13 N-
terminal residues (ΔN-PD); PD without the 23 C-terminal residues (ΔC-PD); and PD without
either the N-or C-terminal residues (ΔNC-PD). A mouse lethality test showed that ΔN-PD …
removal of the 13 N-terminal and 22 C-terminal amino acid residues. In this study, we
examined the toxicity of four constructs: an ε-protoxin derivative (PD), in which a factor Xa
cleavage site was generated at the C-terminal trypsin-sensitive site; PD without the 13 N-
terminal residues (ΔN-PD); PD without the 23 C-terminal residues (ΔC-PD); and PD without
either the N-or C-terminal residues (ΔNC-PD). A mouse lethality test showed that ΔN-PD …