Sperm oxidative stress is detrimental to embryo development: A dose‐dependent study model and a new and more sensitive oxidative status evaluation
LS De Castro, PM De Assis… - Oxidative Medicine …, 2016 - Wiley Online Library
Our study aimed to assess the impact of sperm oxidative stress on embryo development by
means of a dose‐dependent model. In experiment 1, straws from five bulls were subjected to
incubation with increasing H2O2 doses (0, 12.5, 25, and 50 μM). Motility parameters were
evaluated by Computed Assisted System Analysis (CASA). Experiment 2 was designed to
study a high (50 μM) and low dose (12.5 μM) of H2O2 compared to a control (0 μM).
Samples were incubated and further used for in vitro fertilization. Analyses of motility …
means of a dose‐dependent model. In experiment 1, straws from five bulls were subjected to
incubation with increasing H2O2 doses (0, 12.5, 25, and 50 μM). Motility parameters were
evaluated by Computed Assisted System Analysis (CASA). Experiment 2 was designed to
study a high (50 μM) and low dose (12.5 μM) of H2O2 compared to a control (0 μM).
Samples were incubated and further used for in vitro fertilization. Analyses of motility …
[CITATION][C] Sperm oxidative stress is detrimental to embryo development: a dose-dependent study model and a new and more sensitive oxidative status evaluation
LS Castro, PM Assis, AFP Siqueira… - … Medicine and Cellular …, 2016 - repositorio.usp.br
ReP USP - Detalhe do registro: Sperm oxidative stress is detrimental to embryo
development: a dose-dependent study model and a new and more sensitive oxidative
status evaluation … Faculdade de Ciências Farmacêuticas (FCF) Faculdade de Ciências
Farmacêuticas de Ribeirão Preto (FCFRP) …
development: a dose-dependent study model and a new and more sensitive oxidative
status evaluation … Faculdade de Ciências Farmacêuticas (FCF) Faculdade de Ciências
Farmacêuticas de Ribeirão Preto (FCFRP) …
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