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Effect of herbivore stress on transgene behaviour in maize crosses with different genetic backgrounds: cry1Ab transgene transcription, insecticidal protein expression and bioactivity against insect pests
BackgroundDecades after their first commercial release, many theoretical assumptions are still taken for granted in the deployment of genetically...
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ASIS-Seq: Transgene Insertion Site Mapping by Nanopore Adaptive Sampling
Generation of transgenic mice by direct microinjection of foreign DNA into fertilized ova has become a routine technique in biomedical research. It... -
Transgene-Free Genome Editing in Plants
Genome editing has revolutionized genetics and breeding likewise. Especially in plant breeding, it opened new ways to address traits with never known... -
Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons
The Tg(Pax6-cre,GFP) 2Pgr ( α-Cre ) mouse is a commonly used Cre line thought to be retinal-specific. Using targeted locus amplification (TLA), we...
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The human EF1a promoter does not provide expression of the transgene in mice
In this work, we set out to create mice susceptible to the SARS-CoV-2 coronavirus. To ensure the ubiquitous expression of the human ACE2 gene we used...
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Strategic transgene-free approaches of CRISPR-based genome editing in plants
Genome editing through the alteration of nucleotide sequence has already revolutionized the field of site-directed mutagenesis for a decade. However,...
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Use of Fluorescent Protein Reporters for Assessing and Detecting Genome Editing Reagents and Transgene Expression in Plants
Fluorescent protein reporters have been widely used for monitoring the expression of target genes in various engineered organisms. Although a wide... -
Transgene removal using an in cis programmed homing endonuclease via single-strand annealing in the mosquito Aedes aegypti
While gene drive strategies have been proposed to aid in the control of mosquito-borne diseases, additional genome engineering technologies may be...
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Improving adeno-associated viral (AAV) vector-mediated transgene expression in retinal ganglion cells: comparison of five promoters
Recombinant adeno-associated viral vectors (AAVs) are an effective system for gene transfer. AAV serotype 2 (AAV2) is commonly used to deliver...
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Evaluation of the α-casein (CSN1S1) locus as a potential target for a site-specific transgene integration
Transgenic animals are an important tool in biotechnology, including the production of recombinant proteins in the milk. Traditionally, expression...
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Transgene-Free Genome Editing in Nicotiana benthamiana with CRISPR/Cas9 Delivered by a Rhabdovirus Vector
The clustered regularly interspersed short palindromic repeats (CRISPR)/Cas systems have become the most widely adopted genome editing platform owing... -
RAISING is a high-performance method for identifying random transgene integration sites
Both natural viral infections and therapeutic interventions using viral vectors pose significant risks of malignant transformation. Monitoring for...
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Fundamentals of Genetics
Genetics is a dynamic and rapidly advancing field of biology that has found applications in all dimensions of human life. Breakthrough genetic... -
Targeting the AAVS1 Site by CRISPR/Cas9 with an Inducible Transgene Cassette for the Neuronal Differentiation of Human Pluripotent Stem Cells
CRISPR/Cas9 system is a powerful genome-editing technology for studying genetics and cell biology. Safe harbor sites are ideal genomic locations for... -
A transgene-free method for rapid and efficient generation of precisely edited pigs without monoclonal selection
Gene-edited pigs for agricultural and biomedical applications are typically generated using somatic cell nuclear transfer (SCNT). However, SCNT...
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Eye Disease Genetics and Therapeutics
Pathogenesis of many eye diseases can be traced to certain genetic defects at the cell nuclear level and/or due to deficiency or dysfunctions in the... -
Mitochondrial Genetics and Function as Determinants of Bone Phenotype and Aging
Purpose of ReviewThe purpose of this review is to summarize the recently published scientific literature regarding the effects of mitochondrial...
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Loss-of-Function Mutagenesis for Forward Genetics
Genes are central players regulating biological processes. A general strategy for studying gene function is to impair gene activity and examine its... -
Genetics of Growth Control
All animals develop from a fertilized zygote. The development of a zygote depends on cell proliferation for generating tissues and organs. Abnormal... -
Non-Mendelian Genetics: Finding of Transposable Elements in Corns and Flies
The essence of Mendelian genetics is the rule of chromosome segregation and the independent assortment of different chromosomes. However, genes on...