Abstract
Penicillium chrysogenum, recently re-identified as Penicillium rubens, is the microorganism used for the industrial production of penicillin. This filamentous fungus (mold) probably represents the best example of adaptation of a microorganism to industrial production conditions and therefore, it can be considered as a model organism for the study of primary and secondary metabolism under a highly stressful environment. In this regard, biosynthesis and production of benzylpenicillin can be used as an interesting phenotypic trait for those studies. In this chapter, we describe P. chrysogenum culture procedures for the production of benzylpenicillin and the process of antibiotic quantitation either by bioassay or by high-performance liquid chromatography (HPLC).
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Acknowledgments
Authors wish to thank the excellent technical assistance of B. Martín and J. Merino (INBIOTEC, Spain).
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Kosalková, K., Sánchez-Orejas, IC., Cueto, L., García-Estrada, C. (2021). Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC. In: Barreiro, C., Barredo, JL. (eds) Antimicrobial Therapies. Methods in Molecular Biology, vol 2296. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1358-0_11
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DOI: https://doi.org/10.1007/978-1-0716-1358-0_11
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