SVHRSP Alleviates Age-Related Cognitive Deficiency by Reducing Oxidative Stress and Neuroinflammation
<p>SVHRSP reduced memory impairment and improved synaptic functions. (<b>A</b>–<b>H</b>) The effects of SVHRSP on behavioral testing. (<b>A</b>) The escape latency of the passive avoidance experiment; (<b>B</b>) the number of mice that entered the dark room in the passive avoidance test; (<b>C</b>) a device structure-style diagram of the Y Maze; (<b>D</b>) the percentage of spontaneous alternate behavior in the Y-maze trial; (<b>E</b>) the discrimination index of each group of mice in the NOR test; (<b>F</b>) the identification index of each group of mice in the NOR test; (<b>G</b>) the escape latency to reach the platform in the MWM training phase; (<b>H</b>) the number of mice crossing the platform in the MWM probe trials; (<b>I</b>,<b>J</b>) The effects of SVHRSP on long-term potentiation including slope change and amplitude change; (<b>K</b>) The effects of SVHRSP on the synaptic ultrastructure in the hippocampus; The red arrows indicate the presence of synaptic vesicles and postsynaptic dense bodies. (<b>L</b>–<b>N</b>) the representative Western blot bands and the quantification of relative protein expression for SYN and PSD95. The bars represent the mean ± SD. * <span class="html-italic">p</span> < 0.05, ** <span class="html-italic">p</span> < 0.01, <sup>#</sup> <span class="html-italic">p</span> < 0.05, versus the indicated groups.</p> "> Figure 2
<p>SVHRSP downregulated the expression of aging markers. (<b>A</b>,<b>B</b>) Representative SA-β-gal staining images and quantitative analysis in SAMP8 mice. (<b>C</b>) Representative WB bands. (<b>D</b>,<b>E</b>) Quantitative analysis of P21 and P16 in vivo. (<b>F</b>–<b>H</b>) Quantitative analysis of GSH-PX, SOD, and MDA enzyme activity in vivo. (<b>I</b>,<b>J</b>) Representative SA-β-gal staining images and quantitative analysis in H<sub>2</sub>O<sub>2</sub>-induced SH-SY5Y cells. (<b>K</b>) Representative WB bands. (<b>L</b>,<b>M</b>) Quantitative analysis of P21 and p1 in vitro. (<b>N</b>,<b>O</b>) Quantitative analysis of cell cycle arrest in the G0/G1 phase. The cell fraction in the G0/G1 phase, G2/M phase, and S phase was calculated. The bars represent the mean ± SD. * <span class="html-italic">p</span> < 0.05, ** <span class="html-italic">p</span> < 0.01, *** <span class="html-italic">p</span> < 0.001, <sup>#</sup> <span class="html-italic">p</span> < 0.05, versus the indicated groups (<span class="html-italic">n</span> = 3 for each group).</p> "> Figure 3
<p>SVHRSP can inhibit the SIRT1/P53 signaling pathway, leading to enhanced nuclear translocation of NRF-2 and subsequently promoting the expression of antioxidants in SAMP8 mice. (<b>A</b>) Representative WB bands. (<b>B</b>,<b>C</b>) Quantitative analysis of SIRT1 and P53 in vivo. (<b>D</b>) Representative WB bands. (<b>E</b>,<b>F</b>) Quantitative analysis of SIRT1 and P53 in vitro. (<b>G</b>–<b>J</b>) Representative WB bands and quantification analysis of nuclear NRF2 and cytoplasmic NRF2. (<b>K</b>,<b>L</b>) Immunofluorescence images and quantification analysis of NRF2 cells/total cells (scale bar = 10 μm). (<b>M</b>) Representative WB bands. (<b>N</b>,<b>O</b>) Quantitative analysis of SOD 1 and HO-1 in vivo. (<b>P</b>–<b>R</b>) mRNA quantitative analysis of <span class="html-italic">SOD 1</span>, <span class="html-italic">Hmox-1,</span> and <span class="html-italic">NQO1</span> in vivo. (<b>S</b>–<b>W</b>) Representative WB bands and quantitative analysis of SOD 1, HO-1, and NQO 1 in vitro. The bars represent the mean ± SD. ns = not significant, * <span class="html-italic">p</span> < 0.05, ** <span class="html-italic">p</span> < 0.01, *** <span class="html-italic">p</span> < 0.001 versus the indicated groups (<span class="html-italic">n</span> = 3 for each group).</p> "> Figure 4
<p>SVHRSP exerts inhibitory effects on neuroinflammation in SAMP8 mice by modulating the MAPKs/NF-κB signaling pathway. (<b>A</b>–<b>D</b>) Representative Western blot bands and quantitative analysis of cytoplasmic NF-κB p65 and nuclear NF-κB p65. (<b>E</b>–<b>H</b>) Representative Western blot bands and quantification analysis of p-JNK, JNK, p-P38, and P38. (<b>I</b>–<b>K</b>) Quantitative analysis of the serum level of <span class="html-italic">IL-1B</span>, <span class="html-italic">IL-6,</span> and <span class="html-italic">TNF-α</span> measured by using ELISA. (<b>L</b>–<b>N</b>) Quantitative analysis of the mRNA level of <span class="html-italic">IL-1B</span>, <span class="html-italic">IL-6,</span> and <span class="html-italic">TNF-α</span> measured by using QRT-PCR. (<b>O</b>) Representative immunohistochemical images of Iba-1 (left) and GFAP (right). (<b>P</b>,<b>Q</b>) Representative image and quantitative analysis of Nissl staining. The bars represent the mean ± SD. ns = not significant, * <span class="html-italic">p</span> < 0.05, ** <span class="html-italic">p</span> < 0.01, *** <span class="html-italic">p</span> < 0.001, **** <span class="html-italic">p</span> < 0.0001 versus the indicated groups. Scale bar = 50 μm (<span class="html-italic">n</span> = 3 for each group).</p> "> Figure 5
<p>SVHRSP alleviates age-related cognitive deficiency by reducing oxidative stress and neuroinflammation. SVHRSP can enhance the expression of the SIRT1 protein, reduce P53 activity, promote the nuclear translocation of the NRF2 transcription factor, and induce the expression of antioxidant enzymes such as SOD1, NQO1, and HO-1 to counteract oxidative stress. Furthermore, SVHRSP inhibits the expression of senescence markers P16 and P21 proteins. Additionally, by suppressing NF-κB pathway activation, SVHRSP suppresses the release of inflammatory factors IL-1β, TNF-α, and IL-6. (The graphical abstract is depicted by Figdraw 2.0.)</p> ">
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Animals and Treatments
2.3. The Y Maze
2.4. Passive Avoidance Tests
2.5. Novel Object Recognition (NOR)
2.6. Morris Water Maze
2.7. Electrophysiology Experiments
2.8. The Technique of Transmission Electron Microscopy
2.9. Nissl Staining
2.10. Assessment of the Activity of Senescence-Associated β-Galactosidase (SA-β-gal)
2.11. Immunohistochemical and Immunofluorescence Staining
2.12. SOD, MDA, and GSH Assay
2.13. Cell Culture and Treatments
2.14. Cell Cycle Assay
2.15. Enzyme-Linked Immunosorbent Assay (ELISA)
2.16. RNA Extraction and Relative Quantification Using Real-Time Polymerase Chain Reaction (qRT-PCR)
2.17. Western Blot
2.18. Statistics Analysis
3. Results
3.1. SVHRSP Reduced Memory Impairment and Improved Synaptic Functions
3.2. SVHRSP Downregulated the Expression of Aging Markers
3.3. SVHRSP Can Inhibit the SIRT1/P53 Signaling Pathway, Leading to Enhanced Nuclear Translocation of NRF-2 and Subsequently Promoting the Expression of Antioxidants in SAMP8 Mice
3.4. SVHRSP Exerts Inhibitory Effects on Neuroinflammation in SAMP8 Mice by Modulating the MAPKs/NF-κB Signaling Pathway
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Wang, Y.; Wang, Z.; Guo, S.; Li, Q.; Kong, Y.; Sui, A.; Ma, J.; Lu, L.; Zhao, J.; Li, S. SVHRSP Alleviates Age-Related Cognitive Deficiency by Reducing Oxidative Stress and Neuroinflammation. Antioxidants 2024, 13, 628. https://doi.org/10.3390/antiox13060628
Wang Y, Wang Z, Guo S, Li Q, Kong Y, Sui A, Ma J, Lu L, Zhao J, Li S. SVHRSP Alleviates Age-Related Cognitive Deficiency by Reducing Oxidative Stress and Neuroinflammation. Antioxidants. 2024; 13(6):628. https://doi.org/10.3390/antiox13060628
Chicago/Turabian StyleWang, Yingzi, Zhenhua Wang, Songyu Guo, Qifa Li, Yue Kong, Aoran Sui, Jianmei Ma, Li Lu, Jie Zhao, and Shao Li. 2024. "SVHRSP Alleviates Age-Related Cognitive Deficiency by Reducing Oxidative Stress and Neuroinflammation" Antioxidants 13, no. 6: 628. https://doi.org/10.3390/antiox13060628