Supplementary information 3 (Hernández-Ruiz et al.): "Organellar proteomics of human platelet dense granules reveals that 14-3-3z is a granule protein related with atherosclerosis." Information of Protein identifications performed by LC-ESI MS/MS Protein No. 2 Calcium-calmodulin N-terminal domain gi|16974825 Mass: 8483 Score: 54 Queries matched: 4 Chain A, Solution Structure Of Calcium-Calmodulin N-Terminal Domain Observed Mr(expt) Mr(calc) Delta Miss 782.50 1562.99 1562.78 0.20 0 782.55 1563.09 1562.78 0.30 0 782.83 1563.65 1562.78 0.86 0 536.38 1606.12 1605.80 0.32 1 Sequence IDQLTEEQIAEFK IDQLTEEQIAEFK IDQLTEEQIAEFK RDQLTEEQIAEFK 3 14-3-3 protein zeta gi|80477445 Mass: 27899 Score: 198 Queries matched: 5 Tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide Observed Mr(expt) Mr(calc) Delta Miss Sequence 774.73 1547.45 1547.71 -0.26 0 SVTEQGAELSNEER 775.00 1547.99 1547.71 0.28 0 SVTEQGAELSNEER 775.40 1548.79 1547.71 1.08 0 SVTEQGAELSNEER 710.47 1418.93 1417.71 1.21 0 DICNDVLSLLEK 595.58 1189.15 1188.65 0.49 0 DSTLIMQLLR 4 Serum deprivation response protein gi|16741280 Mass: 47202 Score: 53 Queries matched: 1 Serum deprivation response protein [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 803.30 1604.59 1603.70 0.89 Miss Sequence 0 YEGSYALTSEEAER 5 Cyclophilin A gi|10863927 Mass: 18229 Score: 44 Queries matched: 1 peptidylprolyl isomerase A isoform 1 [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 624.49 1246.97 1246.62 0.34 Miss Sequence 1 KITIADCGQLE 7 Heat shock 70kDa protein 8 isoform 1 gi|5729877 Mass: 71082 Score: 43 Queries matched: 2 heat shock 70kDa protein 8 isoform 1 [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 600.58 1199.15 1198.67 0.48 600.87 1199.73 1198.67 1.06 Miss Sequence 0 DAGTIAGLNVLR 0 DAGTIAGLNVLR 8 Heat shock 90 kDa protein gi|306891 Mass: 83584 Score: 50 Queries matched: 4 90kDa heat shock protein Observed Mr(expt) Mr(calc) Delta 639.03 1276.05 1274.64 1.41 Miss Sequence 0 ELISNASDALDK Suppl info 3 (Hernández-Ruiz et al.), Page 1 10' Actin beta gi|14250401 Mass: 41321 Score: 357 Queries matched: 24 actin, beta [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 652.37 1954.09 1953.06 1.03 652.57 1954.69 1953.06 1.63 998.51 997.50 997.48 0.02 566.37 1130.73 1131.52 -0.79 566.84 1131.67 1131.52 0.15 566.88 1131.75 1131.52 0.23 566.93 1131.85 1131.52 0.33 597.78 1790.32 1789.88 0.43 896.17 1790.33 1789.88 0.44 896.26 1790.51 1789.88 0.62 896.36 1790.71 1789.88 0.82 896.57 1791.13 1789.88 1.24 598.10 1791.28 1789.88 1.39 598.18 1791.52 1789.88 1.63 581.46 1160.91 1160.61 0.29 581.48 1160.95 1160.61 0.33 589.46 1176.91 1176.61 0.30 589.49 1176.97 1176.61 0.36 589.52 1177.03 1176.61 0.42 506.38 1516.12 1515.70 0.42 759.07 1516.13 1515.70 0.43 506.39 1516.15 1515.70 0.45 506.39 1516.15 1515.70 0.45 506.55 1516.63 1515.70 0.93 Miss 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Sequence VAPEEHPVLLTEAPLNPK VAPEEHPVLLTEAPLNPK DLTDYLMK GYSFTTTAER GYSFTTTAER GYSFTTTAER GYSFTTTAER SYELPDGQVITIGNER SYELPDGQVITIGNER SYELPDGQVITIGNER SYELPDGQVITIGNER SYELPDGQVITIGNER SYELPDGQVITIGNER SYELPDGQVITIGNER EITALAPSTMK EITALAPSTMK EITALAPSTMK EITALAPSTMK EITALAPSTMK QEYDESGPSIVHR QEYDESGPSIVHR QEYDESGPSIVHR QEYDESGPSIVHR QEYDESGPSIVHR 11 Actinin alpha gi|4501891 Mass: 103563 Score: 126 Queries matched: 5 actinin, alpha 1 [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 715.68 1429.35 1428.76 0.59 769.49 1536.97 1536.77 0.20 769.57 1537.13 1536.77 0.36 804.22 1606.43 1607.80 -1.37 780.56 2338.66 2338.18 0.48 Miss 0 0 0 0 0 Sequence TINEVENQILTR FAIQDISVEETSAK FAIQDISVEETSAK CQLEINFNTLQTK IDQLEGDHQLIQEALIFDNK 12 Adenylyl cyclase-associated protein gi|5453595 Mass: 51926 Score: 74 Queries matched: 2 adenylyl cyclase-associated protein [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 618.55 1235.09 1234.59 0.50 691.87 2072.59 2072.03 0.56 Miss Sequence 0 NSLDCEIVSAK 0 VENQENVSNLVIEDTELK 13' Cofilin-1 non muscle gi|5031635 Mass: 18719 Score: 91 Queries matched: 5 cofilin 1 (non-muscle) [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 668.49 1334.97 1336.62 -1.65 669.53 1337.05 1336.62 0.43 669.55 1337.09 1336.62 0.47 723.06 2166.16 2165.09 1.07 723.21 2166.61 2165.09 1.52 Miss 0 0 0 0 0 Sequence YALYDATYETK YALYDATYETK YALYDATYETK EILVGDVGQTVDDPYATFVK EILVGDVGQTVDDPYATFVK Suppl info 3 (Hernández-Ruiz et al.), Page 2 14 Filamin A gi|57284201 Mass: 283301 Score: 714 Queries matched: 34 filamin A, alpha (actin binding protein 280) [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 708.59 1415.17 1414.74 0.42 708.63 1415.25 1414.74 0.50 708.93 1415.85 1414.74 1.10 614.03 1226.05 1225.76 0.28 643.67 1285.33 1284.72 0.61 827.56 1653.11 1651.85 1.25 934.46 1866.91 1866.84 0.07 614.48 1226.95 1225.65 1.30 550.18 1647.52 1645.87 1.65 893.25 1784.49 1783.88 0.61 893.58 1785.15 1783.88 1.27 672.63 2014.87 2013.98 0.89 553.75 1105.49 1106.60 -1.11 554.80 1107.59 1106.60 0.99 763.24 2286.70 2285.17 1.53 767.45 1532.89 1532.81 0.08 767.62 1533.23 1532.81 0.42 578.46 1154.91 1154.61 0.29 657.16 1312.31 1311.68 0.63 715.64 1429.27 1428.71 0.55 613.42 1224.83 1224.56 0.26 881.93 1761.85 1762.85 -1.00 882.90 1763.79 1762.85 0.94 786.43 1570.85 1569.80 1.05 718.15 1434.29 1433.72 0.57 718.54 1435.07 1433.72 1.35 781.82 2342.44 2341.22 1.22 750.53 1499.05 1499.75 -0.70 751.01 1500.01 1499.75 0.26 751.38 1500.75 1499.75 1.00 816.92 2447.74 2447.24 0.50 818.58 1635.15 1635.70 -0.56 714.06 1426.11 1425.75 0.36 Miss 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Sequence IANLQTDLSDGLR IANLQTDLSDGLR IANLQTDLSDGLR LIALLEVLSQK LPQLPITNFSR VTAQGPGLEPSGNIANK SPYTVTVGQACNPSACR AWGPGLEGGVVGK TGVAVNKPAEFTVDAK VQVQDNEGCPVEALVK VQVQDNEGCPVEALVK CAPGVVGPAEADIDFDIIR LDVQFSGLTK LDVQFSGLTK YTPVQQGPVGVNVTYGGDPIPK SPFSVAVSPSLDLSK SPFSVAVSPSLDLSK IVGPSGAAVPCK VEPGLGADNSVVR AFGPGLQGGSAGSPAR EATTEFSVDAR VANPSGNLTETYVQDR VANPSGNLTETYVQDR GAGTGGLGLAVEGPSEAK ANLPQSFQVDTSK ANLPQSFQVDTSK ASGPGLNTTGVPASLPVEFTIDAK DAGEGGLSLAIEGPSK DAGEGGLSLAIEGPSK DAGEGGLSLAIEGPSK NGQHVASSPIPVVISQSEIGDASR VDINTEDLEDGTCR EAGAGGLAIAVEGPSK 15' Gelsolin isoform a or b gi|4504165 Mass: 86043 Score: 132 Queries matched: 4 gelsolin isoform a [Homo sapiens] Observed Mr(expt) Mr(calc) Delta 660.45 1318.89 1318.69 0.20 660.51 1319.01 1318.69 0.32 660.60 1319.19 1318.69 0.50 833.42 1664.83 1664.77 0.05 Miss 0 0 0 1 Sequence AGALNSNDAFVLK AGALNSNDAFVLK AGALNSNDAFVLK DSQEEEKTEALTSAK 16 Kindin1 gi|41281905 Mass: 76475 Score: 105 Queries matched: 3 UNC-112 related protein 2 long form [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 514.82 1027.63 1027.59 0.03 888.18 1774.35 1775.86 -1.51 889.25 1776.49 1775.86 0.63 Miss 0 0 0 Sequence IDLAVGDVVK VFVGEEDPEAESVTLR VFVGEEDPEAESVTLR 17 Myosin light chain 2 gi|188586 Mass: 19914 Score: 54 Queries matched: 1 myosin light chain 2 Mr(calc) Delta Observed Mr(expt) 615.00 1227.99 1227.61 0.38 Miss Sequence 0 LNGTDPEDVIR Suppl info 3 (Hernández-Ruiz et al.), Page 3 17' Myosin light chain 3 gi|188590 Mass: 17091 Score: 56 Queries matched: 1 myosin light chain 3 Mr(calc) Delta Observed Mr(expt) 677.90 1353.79 1354.71 -0.92 678.03 1354.05 1354.71 -0.66 678.08 1354.15 1354.71 -0.56 Miss 0 0 0 Sequence ALGQNPTDAEVLK ALGQNPTDAEVLK ALGQNPTDAEVLK 18 Myosin, heavy polypep. 9 gi|12667788 Mass: 227646 Score: 304 Queries matched: 11 myosin, heavy polypeptide 9, non-muscle [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 776.48 1550.95 1550.69 0.26 603.66 1205.31 1204.63 0.67 597.50 1192.99 1192.61 0.38 827.68 1653.35 1652.78 0.57 827.93 1653.85 1652.78 1.07 678.95 2033.83 2032.94 0.88 602.95 1203.89 1203.58 0.30 655.01 1962.01 1960.91 1.10 655.02 1962.04 1960.91 1.13 675.04 1348.07 1347.62 0.45 825.14 2472.40 2471.17 1.23 Miss 0 0 0 0 0 0 0 0 0 0 0 Sequence EDQSILCTGESGAGK TDLLLEPYNK ALELDSNLYR IAEFTTNLTEEEEK IAEFTTNLTEEEEK ELESQISELQEDLESER ALEQQVEEMK TQLEELEDELQATEDAK TQLEELEDELQATEDAK DELADEIANSSGK IAQLEEELEEEQGNTELINDR 19 Pleckstrin gi|4505879 Mass: 40456 Score: 49 Queries matched: 1 pleckstrin [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 699.56 1397.11 1396.63 0.48 Miss Sequence 0 GSTLTSPCQDFGK 20' Profilin I gi|4826898 Mass: 15216 Score: 187 Queries matched: 9 profilin 1 [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 607.43 1212.85 1212.61 0.23 607.44 1212.87 1212.61 0.25 607.91 1213.81 1212.61 1.19 822.69 1643.37 1642.93 0.44 735.96 1469.91 1469.75 0.15 736.07 1470.13 1469.75 0.37 736.52 1471.03 1469.75 1.27 690.57 1379.13 1378.71 0.42 690.72 1379.43 1378.71 0.72 Miss 0 0 0 0 0 0 0 0 0 Sequence DSPSVWAAVPGK DSPSVWAAVPGK DSPSVWAAVPGK TFVNITPAEVGVLVGK SSFYVNGLTLGGQK SSFYVNGLTLGGQK SSFYVNGLTLGGQK STGGAPTFNVTVTK STGGAPTFNVTVTK Suppl info 3 (Hernández-Ruiz et al.), Page 4 21 Talin 1 gi|16753233 Mass: 271667 Score: 739 Queries matched: 34 talin 1 [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 744.42 1486.83 1485.78 1.04 708.51 2122.51 2121.18 1.33 708.54 2122.60 2121.18 1.42 708.56 2122.66 2121.18 1.48 714.57 2140.69 2139.10 1.59 729.05 1456.09 1455.83 0.26 728.58 1455.15 1454.76 0.39 728.58 1455.15 1454.76 0.39 747.12 1492.23 1491.88 0.35 919.29 2754.85 2753.40 1.45 529.43 1056.85 1056.59 0.25 824.09 2469.25 2468.30 0.95 1014.12 2026.23 2026.04 0.18 676.47 2026.39 2026.04 0.34 677.01 2028.01 2026.04 1.96 599.49 1196.97 1196.64 0.33 810.34 1618.67 1619.78 -1.11 811.42 1620.83 1619.78 1.05 811.81 1621.61 1619.78 1.83 773.33 2316.97 2316.12 0.85 773.65 2317.93 2316.12 1.81 892.23 1782.45 1781.93 0.52 595.21 1782.61 1781.93 0.68 734.98 1467.95 1467.67 0.28 735.04 1468.07 1467.67 0.40 708.90 1415.79 1415.77 0.01 709.21 1416.41 1415.77 0.63 787.11 1572.21 1571.77 0.44 787.54 1573.07 1571.77 1.30 968.57 1935.13 1934.91 0.21 516.34 1030.67 1030.60 0.06 770.85 2309.53 2309.18 0.35 730.74 1459.47 1458.79 0.68 731.02 1460.03 1458.79 1.24 Miss 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 Sequence EVIQEWNLTNIK LLAALLEDEGGSGRPLLQAAK LLAALLEDEGGSGRPLLQAAK LLAALLEDEGGSGRPLLQAAK VVAPTISSPVCQEQLVEAGR ILAQATSDLVNAIK ASAGPQPLLVQSCK ASAGPQPLLVQSCK AVAEQIPLLVQGVR GSQAQPDSPSAQLALIAASQSFLQPGGK NLGTALAELR GVAALTSDPAVQAIVLDTASDVLDK LNEAAAGLNQAATELVQASR LNEAAAGLNQAATELVQASR LNEAAAGLNQAATELVQASR ALSTDPAAPNLK NGNLPEFGDAISTASK NGNLPEFGDAISTASK NGNLPEFGDAISTASK DKAPGQLECETAIAALNSCLR DKAPGQLECETAIAALNSCLR DLDQASLAAVSQQLAPR DLDQASLAAVSQQLAPR AGALQCSPSDAYTK AGALQCSPSDAYTK LAQAAQSSVATITR LAQAAQSSVATITR QELAVFCSPEPPAK QELAVFCSPEPPAK EADESLNFEEQILEAAK SIAAATSALVK VGAIPANALDDGQWSQGLISAAR QVAASTAQLLVACK QVAASTAQLLVACK 22 Transgelin 2 gi|12803567 Mass: 22563 Score: 100 Queries matched: 4 TAGLN2 protein [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 608.16 1214.31 1214.68 -0.38 797.22 1592.43 1593.71 -1.28 797.99 1593.97 1593.71 0.26 798.02 1594.03 1593.71 0.32 Miss 0 0 0 0 Sequence TLMNLGGLAVAR DDGLFSGDPNWFPK DDGLFSGDPNWFPK DDGLFSGDPNWFPK Miss 0 0 0 0 0 0 0 0 0 Sequence IQVLQQQADDAEER IQVLQQQADDAEER IQVLQQQADDAEER EQAEAEVASLNR EQAEAEVASLNR IQLVEEELDR IQLVEEELDR IQLVEEELDR IQLVEEELDR 23'' Tropomyosin isoform gi|9508585 Mass: 29109 Score: 227 Queries matched: 9 tropomyosin isoform [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 822.11 1642.21 1641.80 0.41 822.41 1642.81 1641.80 1.01 822.41 1642.81 1641.80 1.01 658.35 1314.69 1315.64 -0.95 659.52 1317.03 1315.64 1.39 622.49 1242.97 1242.65 0.32 622.52 1243.03 1242.65 0.38 622.52 1243.03 1242.65 0.38 622.59 1243.17 1242.65 0.52 Suppl info 3 (Hernández-Ruiz et al.), Page 5 24' Vinculin isoform VCL gi|4507877 Mass: 117220 Score: 332 Queries matched: 40 vinculin isoform VCL [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 702.95 1403.89 1403.71 0.17 736.10 1470.19 1469.67 0.51 729.53 1457.05 1456.79 0.26 729.67 1457.33 1456.79 0.54 553.48 1104.95 1104.60 0.34 658.06 1314.11 1313.73 0.38 658.14 1314.27 1313.73 0.54 615.06 1228.11 1229.57 -1.46 743.04 1484.07 1483.80 0.27 586.52 1171.03 1172.64 -1.61 587.38 1172.75 1172.64 0.11 587.44 1172.87 1172.64 0.23 586.05 1170.09 1169.64 0.45 635.55 1269.09 1268.67 0.41 635.60 1269.19 1268.67 0.51 566.88 1131.75 1131.57 0.17 Miss 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Sequence ETVQTTEDQILK DPSASPGDAGEQAIR AQQVSQGLDVLTAK AQQVSQGLDVLTAK SLGEISALTSK QVATALQNLQTK QVATALQNLQTK WIDNPTVDDR VDQLTAQLADLAAR ALASQLQDSLK ALASQLQDSLK ALASQLQDSLK ELTPQVVSAAR AVAGNISDPGLQK AVAGNISDPGLQK TNLLQVCER 25' Aldolase A gi|28614 Mass: 39706 Score: 155 Queries matched: 4 aldolase A [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 666.87 1331.73 1331.69 0.03 667.01 1332.01 1331.69 0.31 667.12 1332.23 1331.69 0.53 667.13 1332.25 1331.69 0.55 Miss 0 0 0 0 Sequence GILAADESTGSIAK GILAADESTGSIAK GILAADESTGSIAK GILAADESTGSIAK 26 Enolase 3 gi|16878083 Mass:47244 Score: 50 Queries matched: 1 Enolase 3 [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 818.13 1634.25 1632.81 1.43 Miss Sequence 0 VNQIGSVTESIQACK 27 GAPDH gi|31645 Mass: 36202 Score: 156 Queries matched: 4 glyceraldehyde-3-phosphate dehydrogenase [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 766.14 1530.27 1529.79 0.48 766.14 1530.27 1529.79 0.48 807.45 1612.89 1612.89 -0.01 917.72 1833.43 1832.91 0.51 Miss 0 0 0 0 Sequence VPTANVSVVDLTCR VPTANVSVVDLTCR LVINGNPITIFQER IISNASCTTNCLAPLAK 28 Lactate dehydrogenase B gi|54696396 Mass: 36900 Score: 76 Queries matched: 3 lactate dehydrogenase B [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 816.21 1630.41 1628.85 1.55 625.01 1248.01 1247.59 0.41 625.01 1248.01 1247.59 0.41 Miss 0 0 0 Sequence SLADELALVDVLEDK VIGSGCNLDSAR VIGSGCNLDSAR Suppl info 3 (Hernández-Ruiz et al.), Page 6 29' Pyruvate kinase gi|35505 Mass: 58411 Score: 175 Queries matched: 10 pyruvate kinase [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 599.54 1197.07 1196.64 0.43 680.35 1358.69 1358.70 -0.01 680.42 1358.83 1358.70 0.13 680.46 1358.91 1358.70 0.21 680.51 1359.01 1358.70 0.31 890.62 1779.23 1778.87 0.36 890.97 1779.93 1778.87 1.06 818.99 1635.97 1635.88 0.08 571.45 1140.89 1140.60 0.28 611.44 1220.87 1220.63 0.24 Miss 0 0 0 0 0 0 0 0 0 0 31 Coagulation factor XIII A chain precursor gi|119720 Mass: 83728 Score: 48 Queries matched: 5 Coagulation factor XIII A chain precursor (Coagulation factor XIIIa) Mr(calc) Delta Miss Observed Mr(expt) 590.53 1179.05 1178.60 0.45 0 664.08 1326.15 1325.82 0.33 0 664.16 1326.31 1325.82 0.49 0 763.54 1525.07 1524.77 0.29 0 764.11 1526.21 1524.77 1.43 0 Sequence LDIDSPPITAR NTGIICTIGPASR NTGIICTIGPASR NTGIICTIGPASR NTGIICTIGPASR GADFLVTEVENGGSLGSK GADFLVTEVENGGSLGSK GVNLPGAAVDLPAVSEK GDLGIEIPAEK CCSGAIIVLTK Sequence LIASMSSDSLR STVLTIPEIIIK STVLTIPEIIIK ETFDVTLEPLSFK ETFDVTLEPLSFK 32 Fibrinogen alpha gi|223918 Mass: 49708 Score: 60 Queries matched: 1 fibrinogen alphaA Mr(calc) Delta Observed Mr(expt) 768.97 1535.93 1535.69 0.24 Miss Sequence 0 ADSGEGDFLAEGGGVR 33' Fibrinogen beta precursor gi|182430 Mass: 55545 Score: 50 Queries matched: 2 beta-fibrinogen precursor Observed Mr(expt) Mr(calc) Delta 810.02 1618.03 1617.79 0.24 810.37 1618.73 1617.79 0.94 Miss Sequence 0 TPCTVSCNIPVVSGK 0 TPCTVSCNIPVVSGK 35 Ig gamma-1 chain C region gi|121039 Mass: 36596 Score: 45 Queries matched: 1 Ig gamma-1 chain C region Mr(calc) Delta Observed Mr(expt) 661.49 1320.97 1320.67 0.29 Miss Sequence 0 STSGGTAALGCLVK 36 Platelet factor 4 gi|34810164 Mass: 7992 Score: 101 Queries matched: 4 Chain D, Crystal Structure Of Platelet Factor 4 Mr(calc) Delta Observed Mr(expt) 667.52 1333.03 1332.71 0.31 833.52 1665.03 1664.70 0.32 833.58 1665.15 1664.70 0.44 770.37 1538.73 1538.71 0.02 Miss 0 0 0 0 Sequence ICLDLQAPLYK EAEEDGDLQCLCVK EAEEDGDLQCLCVK EESLDSDLYAELR Suppl info 3 (Hernández-Ruiz et al.), Page 7 38 Pro-platelet basic protein precursor gi|4505981 Mass: 14171 Score: 125 Queries matched: 8 pro-platelet basic protein precursor [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 528.79 1055.57 1055.51 0.06 528.79 1055.57 1055.51 0.06 528.82 1055.63 1055.51 0.12 550.87 1099.73 1099.62 0.10 550.88 1099.75 1099.62 0.12 550.96 1099.91 1099.62 0.28 551.31 1100.61 1099.62 0.98 593.00 1183.99 1183.60 0.38 Miss 0 0 0 0 0 0 0 1 Sequence ICLDPDAPR ICLDPDAPR ICLDPDAPR NIQSLEVIGK NIQSLEVIGK NIQSLEVIGK NIQSLEVIGK KICLDPDAPR 39' Serum albumin gi|28592 Mass: 71316 Score: 101 Queries matched: 3 serum albumin [Homo sapiens] Mr(calc) Delta Observed Mr(expt) 575.34 1148.67 1148.61 0.06 722.49 1442.97 1442.63 0.33 722.58 1443.15 1442.63 0.51 Miss 0 0 0 Sequence LVNEVTEFAK YICENQDSISSK YICENQDSISSK 40 Thrombospondin-1 precursor gi|135717 Mass: 133321 Score: 175 Queries matched: 8 Thrombospondin-1 precursor Observed Mr(expt) Mr(calc) Delta 604.51 1207.01 1206.62 0.38 697.18 1392.35 1393.72 -1.38 698.20 1394.39 1393.72 0.66 623.34 1244.67 1245.69 -1.03 624.42 1246.83 1245.69 1.13 667.11 1332.21 1331.63 0.58 826.56 1651.11 1650.70 0.41 787.97 1573.93 1572.66 1.26 Miss 0 0 0 0 0 0 0 0 Sequence SITLFVQEDR FVFGTTPEDILR FVFGTTPEDILR TIVTTLQDSIR TIVTTLQDSIR LCNNPTPQFGGK DCVGDVTENQICNK DNCQYVYNVDQR Suppl info 3 (Hernández-Ruiz et al.), Page 8