A) Schematic of the ChemLTP protocol. Dissociated hippocampal neuronal cultures (DIV 17–20) were treated with glycine (200 µM) for 3 min, then incubated in media without glycine for 5, 20 or 40 min. B) ChemLTP increases surface GluA1. Graph shows quantification of the ratio of surface to total GluA1. Statistical significance was determined using ANOVA. n = 5 ***p<0.005, ***p<0.001. C) ChemLTP Increases surface expression of endogenous GluA1-containing AMPARs in dissociated hippocampal neurons expressing EGFP. Surface and internal GluA1 were sequentially labeled under non-permeabilised and permeabilised conditions, respectively, with different coloured secondary antibodies. Representative images are shown. Graph shows surface:internal ratio relative to unstimulated control. n = 12–15 cells per condition in 3 independent experiments. ANOVA **p<0.005 and ***p<0.001. Scale bars – 20 µm.