Publications - JLRG

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Publications

1. J.Y. Kim, J.A. Young, N.W. Gunther IV, and J.L. Lee. Inhibition of Salmonella by

bacteriocin-producing lactic acid bacteria derived from US Kimchi and broiler

chicken. Journal of Food Safety. 2014. Accepted.

2. M.E. Hickey, G.M. Accumanno, D.M. McIntosh, G.S. Blank, and J.L. Lee.

Comparison of extracellular DNase- and protease-producing spoilage bacteria

isolated from Delaware pond-sourced and retail channel catfish (Ictalurus

punctatus). Journal of the Science of Food and Agriculture. 2014. Accepted.

3. M.E. Hickey, G.P. Richards, and J.L. Lee. Development of a two-step, non-

probed multiplex real-time PCR for surveilling Vibrio anguillarum in seawater.

Journal of fish diseases. 2014. DOI: 10.1111/jfd.12264.

4. J.Y. Kim and J.L. Lee. Multipurpose assessment for the quantification of Vibrio

spp. and total bacteria in fish and seawater using multiplex real-time PCR. Journal

of the Science of Food and Agriculture. 2014. DOI: 10.1002/jsfa.6699.

5. M.E. Hickey, S.A. Besong, V. Kalavacharla, and J.L. Lee. Identification of

extracellular DNase producing bacteria populations on catfish filets during

refrigerated storage. Food Science and Biotechnology. 2013. 22:87-92.

6. T. Lingham, S.A. Besong, G. Ozbay and J.L. Lee. Antimicrobial activity of

vinegar on bacterial species isolated from retail and local channel catfish

(Ictalurus punctatus). Journal of Food Processing and Technology. 2012. DOI:

10.4172/2157-7110.S11-001.

7. K.D. Maull, M.E. Hickey, and J.L. Lee. The Study and Identification of Bacterial

Spoilage Species Isolated from Catfish during Refrigerated Storage. Journal of

Food Processing and Technology. 2012. DOI: 10.4172/2157-7110.S11-003.

8. S.C. Moon and J.L Lee. Electrospun poly (ethylene oxide)/hydrated iron oxide

/sodium alginate composite nanofibers with multi-functional properties. Polymer

Engineering and Science. 2013. 53: 1321-1326.

9. J.L. Lee and R.E. Levin. Detection of 5 CFU/g of Escherichia coli O157:H7 on

lettuce using activated charcoal and real-time PCR without enrichment. 2011.

Food Microbiology. 2011. 28: 562-567.

10. J.L. Lee and R.E. Levin. Selective detection of mixed bacterial survivors from fish filets after freezing and thawing by ethidium bromide monoazide real-time PCR. Food Biotechnology. 2010. 24: 270-281.

11. J.L. Lee and R.E. Levin. Discrimination of viable and dead Vibrio vulnificus after

refrigerated and frozen storage using EMA, sodium deoxycholate and real-time

PCR. Journal of Microbiological Methods. 2009. 79: 184-188.

12. J.L. Lee and R.E. Levin. Improved detection of g-irradiated Vibrio vulnificus after heat and cold shock treatment by using ethidium monoazide real-time PCR. Food Science and Biotechnology. 2009. 18: 788-792.

13. J.L. Lee and R.E. Levin. Detection of Vibrio vulnificus before and after g-

irradiation in quahog clams (Mercenaria mercenaria) tissue using real-time

polymerase chain reaction. Food Biotechnology. 2009. 23: 121-132.

14. J.L. Lee and R.E. Levin. A comparative study of the ability of EMA and PMA to

distinguish viable from heat killed mixed bacterial flora from fish fillets. Journal of

Microbiological Methods. 2009. 76: 93-96.

15. J.L. Lee and H.Y. Kim. Expression and iron storage properties of recombinant

human ferritins in Escherichia coli:Relevance for functional ferritins in food

systems. Food Biotechnology. 2008. 22: 140-149.

16. J.L. Lee and R.E. Levin. New approach for discrimination of g-irradiated Vibrio vulnificus by using Real-time polymerase chain reaction. Journal of Microbiological Methods. 2008. 73: 1-6.

17. J.L. Lee and R.E. Levin. and H. Y. Kim. Improved co-expression and multi-assembly properties of recombinant human ferritins subunit in Escherichia coli. J. Microbiol. Biotechnol. 2008. 18: 926-932.

18. J.L. Lee and R.E. Levin. Discrimination of g-irradiated Vibrio vulnificus by using ethidium monoazide real-time polymerase chain reaction. Journal of Applied Microbiology. 2008. 104: 728-734.

19. J.L. Lee and R.E. Levin. Quantification of total viable bacteria on fish fillets by using ethidium monoazide Real-time polymerase chain reaction. International Journal of Food Microbiology. 2007. 118: 312-317.

20. J.L. Lee., C.S. Park., and H.Y. Kim. Functional assembly of recombinant human ferritin subunits in Pichia pastoris. J. Microbiol. Biotechnol. 2007. 17: 1695-1699.

21. J.L. Lee and R.E. Levin. Rapid quantification of total bacteria on cod fillets by using real-time PCR. Journal of Fisheries Sciences. 2007. 1: 58-67.

22. J.L. Lee and R.E. Levin. Selection of universal primer for quantification of total bacteria associated with fish fillets. Food Biotechnology. 2006. 20: 275- 285.

23. J.L. Lee and R.E. Levin. Direct application of the polymerase chain reaction for quantification of total bacteria on fish fillets. Food Biotechnology. 2006. 20: 287-298.

24. J.L. Lee and R.E. Levin. Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction. Journal of Microbiological Methods. 2006. 67: 456- 462.

25. J. L. Lee, S.N. Yang, C. S. Park, D. Jeoung, and H. Y. Kim. Purification and its

glycosylation pattern of recombinant L-ferritin in Pichia pastoris. J. Microbiol.

Biotechnol. 2004. 14: 68-73.

26. J. L. Lee, H. S. Song, H, J. Kim, J. H. Park., D. K. Chung., C. S. Park., D. Jeoung., and H. Y. Kim. Functional expression and production of human H-ferritin in Pichia pastoris. Biotechnology Letters. 2003. 25:1019-1023.

27. S. N. Yang, H. S. Song., J. L. Lee and H. Y. Kim. Selection of enhanced iron

uptake yeast mutants by EMS mutagenesis. Kor. J. Microbiol. Biotechnol. 2003.

31: 435-438.

28. J. L. Lee., J. H. Park., I. S. Jung., D, Jeoung., S. K. Cho., H. Y. Kim. Purification

and characterization of the recombinant human Iron Regulatory Protein 2

expressed in Escherichia. coli. Agric. Chem.Biotechnol. 2002. 45:117-120.

29. J. L. Lee, J. H. Park and H. Y. Kim. Identification of differentially expressed genes using RNA differential display. Agric. Chem.Biotechnol. 1999. 42: 123-127.

30. H. Y. Kim, I. S. Jung, and J. L. Lee. Regulation of ferritin synthesis in 5’-

untranslated region by Iron Responsive Element. Agric. Chem.Biotechnol. 1998.

41: 224-227.

Presentations

1. J.L. Lee. Globalization of Korean Traditional Foods: Isolation of lactic acid

bacteria from homemade Kimchi in Delmarva region and adoption if its probiotics

properties for poultry and food safety in the USA. Korean Society of Food

Science and Technology, Annual meeting. (2013, Cheonan, Korea).

2. J.L. Lee and J.Y. Kim. Optimization of mutiplex real-time PCR assay for detection

and quantification of Vibrio spp. and total bacteria. IAFP Annual meeting (2013,

Charlotte, USA).

3. J.L. Lee., G. Accumanno. and M.E. Hickey. Isolation of DNase and protease

producing bacteria on catfish spoilage. IAFP Annual meeting (2013, Charlotte,

USA).

4. J.L. Lee and M.E. Hickey. Training and development of research-savvy

undergraduate student through the multiplex real-time PCR assay. NACTA

conference (2013, Blacksburg, USA).

5. J.L. Lee. Assaying the viability of spoilage bacteria and selectivity of r-irradiated

Vibrio spp. in seafood using a PCR assay based on DNA amplification. Korean

Society of Food Science and Technology Annual meeting (2011, Daegu, Korea).

6. J. L. Lee. Microbial evaluation of the freshness of cod fillet using real-time PCR

for quantification of bacteria on fish fillets. UKC Annual meeting. (2009, Raleigh,

USA). 7. J. L. Lee and R. E. Levin. Selective detection of mixed bacterial survivors from

fish fillets after freezing and thawing by ethidium bromide monoazide real-time

PCR. IFT Annual meeting.(2009,Anaheim,USA).

8. J. L. Lee and R. E. Levin. Discrimination of gamma (r)-irradiated & non irradiated

Vibrio vulnificus in quahog clams (Mercenaria mercenaria) using real-time PCR.

IFT Annual meeting. (2008, New Orleans,USA).

9. J. L. Lee and R. E. Levin. Discrimination of g-irradiated & non irradiated Vibrio

vulnificus in quahog clams (Mercenaria mercenaria) using Real-Time Polymerase

Chain Reaction. Annual Atlantic Fisheries Technology Conference. (2007,

Portland, USA).

10. J. L. Lee and R. E. Levin. Discrimination of g-irradiated & non r-irradiated Vibrio vulnificus using Real-Time Polymerase Chain Reaction. IFT Annual meeting. (2007, Chicago. USA).

11. J. L. Lee. Use of ethidium bromide monoazide (EMA) for quantification of viable and dead bacteria from fish fillets by PCR and real-time PCR. Cambridge Healthtech Institute’s nucleic acid-based technologies conference. (2007, Baltimore, USA).

12. J. L. Lee and R. E. Levin. Quantification of total viable bacteria on fish fillets by

using PCR and EMA real-time PCR. Trans-Atlantic Fisheries Technology

Conference.(2006,Quebec, Canada).

13. J. L. Lee and R. E. Levin. Rapid quantification of viable total bacteria on fish

fillets by using ethidium bromide monoazide real-time PCR. IFT Annual meeting.

(2006, Orlando. USA).

14. J.L. Lee, and H. Y. Kim. Expression of recombinant human H-ferritin in Pichia

pastoris. CHI Conference (2004, San Diego, USA).

15. J.L. Lee, and H. Y. Kim. Functional expression of human L-ferritin in Pichia pastoris. Annual- Meeting of the Korean Society for Microbiology and Biotechnology, Bio Exhibition &International Symposium. (2003, Muju, Korea).

16. J.L. Lee, and H. Y. Kim. Expression of human H-and L-ferritin in Pichia pastoris. Annual Meeting of the Korean society for microbiology and biotechnology. (2001, Fall, Sang-Rok, Korea).

17. J.L. Lee, and H. Y. Kim. The constriction of bicistronic expression vector for

studying human ferritin heteropolymers. Annual Meeting of the Korean Society

for Applied Biological Chemistry. (2000, Spring, Young-Nam National Univ,

Korea).

18. J.L. Lee, and H. Y. Kim. Overexpression of recombinant ferritins in E. coli. Annual Meeting of the Korean Society of Food Science & Technology. (1999, Fall, Kyung-Hee Univ, Korea).

19. J.L. Lee, and H. Y. Kim. Characterization of the recombinant Human H-L-Ferritin. Annual Meeting of the Korean Society for Applied Biological Chemistry. (1999, Spring, Kang-Won National Univ, Korea).

20. J.L. Lee, J. H. Park, and H. Y. Identification of differentially expressed genes using RNA differential display. Annual Meeting of the Korean Society for Biochemistry and Molecular Biology. (1999, Spring, A-Ju Univ, Korea).

21. J.L. Lee and H. Y. Kim. Expression and purification of human Ferritin in

Escherichia. coli. Annual Meeting of the Korean Society for Biochemistry and

Molecular Biology. (1999, Spring, A-Ju Univ, Korea).

22. J.L. Lee and H. Y. Kim. Characterization of Iron-regulated protein in iron

metabolism. Annual Meeting of the Korean Society for Molecular and Cellular

Biology. (1998, Seoul National Univ, Korea).

23. J.L. Lee and H. Y. Kim. Characterization of Nramp2 as an iron transport protein.

Annual Meeting of the Korean Society for Biochemistry and Molecular Biology.

(1998, Fall, KAIST, Korea).

24. J.L. Lee and H. Y. Kim. Structure and function of the iron responsive element.

Annual Meeting of the Korean Society for Biochemistry and Molecular Biology.

(1998, Spring, Korea Univ, Korea).

25. J.L. Lee and H. Y. Kim. Binding characterization of iron regulatory protein to

RNA ligand. Annual Meeting of the Korean Society for Applied Biological

Chemistry. (1997, Seoul National Univ, Korea).

Contact

Jung-lim Lee, Ph.D.
Associate Professor in Food Microbiology and Food Safety,

Food Science Program,
Department of Human Ecology,

1200 N. DuPont Highway, Dover, DE, 19901

Fax: (302) 857-6441
jlee@desu.edu

Food Microbiology Lab

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