- When Captain America is giving orders, he says "Hulk, smash," A catch phrase uttered by the Hulk in the comics.
- After the Hulk thrashes Loki, he says "Puny god," a reference to another oft-repeated Hulk phrase, "Puny humans."
- Scarlett Johansson has appeared in Ghost World - Mundo Fantasma (2001) and The Spirit (2008)
- Chris Evans appeared in Quarteto Fantástico (2005), Tartarugas Ninja: Uma Nova Aventura (2007), Os Perdedores (2010) and Scott Pilgrim Contra o Mundo (2010)
- Powers Boothe (who provides a cameo in the film) previously appeared in Sin City: Cidade do Pecado (2005)