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35S-labeled probe Description: In Situ Hybridization using 35S-labeled probe, Detection by emulsion or film autoradiography. Frozen Sections. Added on: 23-Nov-2004 Hits: 4986 Rating: 10 (1 Vote) Rate this Site | Details
Anderson Lab In Situ Hybridization Protocols Description: In Situ Hybridization of Frozen Sections, Whole Mount In Situ Hybridization, In Situ Hybridization on Cultured Cells and Additional Techniques Added on: 07-Oct-2004 Hits: 13250 Rating: 6 (2 Votes) Rate this Site | Details
Chick, mouse, and Xenopus two colour whole mount ISH Description: Protocols from Oxford Practical Approach Series.
Chick, mouse, and Xenopus two colour whole mount in situ hybridization staining with Fast Red and TrueBlue Added on: 07-Oct-2004 Hits: 3252 Rating: 1 (1 Vote) Rate this Site | Details
Detection of mRNA by in situ hybridization Description: The following page describes the current favorite non-radioactive in situ hybridization technique used by Dr. G.V. Childs at the University of Arkansas for Medical Sciences to detect mRNAs in whole pituitary cells grown in culture. Added on: 07-Oct-2004 Hits: 11927 Rating: 10 (2 Votes) Rate this Site | Details
DIG-labeled probe Description: In Situ Hybridization using DIG-labeled probe, Detection by AP (alkaline phosphatase), FITC or rhodamine. Paraffin Sections. Added on: 23-Nov-2004 Hits: 4427 Rating: 10 (2 Votes) Rate this Site | Details
DNA: DNA in situ hybridization Description: Procedures for in situ hybridization of labeled DNA probes to spread or sectioned chromosomes and nuclei (DNA) are described here. Double-target experiments are recommended for most applications, while multiple-target or reprobing of preparations are possible if more information is required. Added on: 17-Dec-2009 Hits: 409 Rate this Site
FISH protocols for Drosophila Description: Fluorescent in situ hybridization (FISH) is the standard method for visualizing the
spatial distribution of RNA. While traditional histochemical RNA detection methods
suffered from limitations in resolution or sensitivity, the recent development of
peroxidase-mediated tyramide signal amplification (TSA) provides strikingly enhanced
sensitivity and subcellular resolution. In this chapter, we describe optimized FISH
protocols for Drosophila embryos and tissues utilizing TSA, either for single genes or in
a high-throughput format, which greatly increase the sensitivity, consistency, economy
and throughput of the procedure. We also describe variations of the method for RNARNA
and RNA-protein co-detection. Added on: 19-Dec-2007 Hits: 664 Rate this Site
Fluorescent in situ hybridization (FISH) Description: ULS(TM)-dGreen and ULS(TM)-Rhodamine labeled probes for translocation, locus specific and
chromosome copy number detection Added on: 10-Dec-2004 Hits: 1164 Rate this Site
GreenStar* BIOTIN-labeled probe Description: In Situ Hybridization using GreenStar* BIOTIN-labeled probe in paraffin sections. Detection by AP (alkaline phosphatase) or HRP (Horseradish Peroxidase) with or without tyramide signal amplification (TSA). Added on: 23-Nov-2004 Hits: 1062 Rate this Site
GreenStar* BIOTIN-labeled probe Description: In Situ Hybridization using GreenStar* BIOTIN-labeled probe in frozen sections. Detection by AP (alkaline phosphatase) or HRP (Horseradish Peroxidase) with or without tyramide signal amplification (TSA). Added on: 23-Nov-2004 Hits: 846 Rate this Site
GreenStar* DIG-labeled probe Description: In Situ Hybridization using GreenStar* DIG-labeled probe in paraffin sections. Detection by direct fluorescence, AP (alkaline phosphatase) or tyramide signal amplification (TSA). Added on: 23-Nov-2004 Hits: 1059 Rate this Site
GreenStar* DIG-labeled probe Description: In Situ Hybridization using GreenStar* DIG-labeled probe in frozen sections. Detection by direct fluorescence, AP (alkaline phosphatase) or tyramide signal amplification (TSA). Added on: 23-Nov-2004 Hits: 908 Rate this Site
GreenStar* FITC-labeled probe Description: In Situ Hybridization using GreenStar* FITC-labeled probe, Detection by direct fluorescence, AP (alkaline phosphatase) or tyramide signal amplification (TSA). Frozen Sections. Added on: 23-Nov-2004 Hits: 856 Rate this Site
In Situ Hybridization Detection Systems (Vector Laboratories) Description: Several different fluorescent or enzyme-based systems are used for detecting labeled nucleic acid probes. These
options provide the researcher with flexibility in optimizing experimental systems to achieve highest sensitivity, to
avoid potential problems such as endogenous biotin or enzyme activity, or to introduce multiple labels in a single
experiment. Such factors as tissue fixation, endogenous biotin or enzyme activity, desired sensitivity, and permanency
of record should all be considered when choosing both the optimal probe label and subsequent detection system. Added on: 07-Oct-2004 Hits: 1309 Rate this Site
In Situ Hybridization with Nanogold®-Streptavidin Description: The small size of Nanogold® probes, their lack of aggregation and their consequent ability to access nuclear antigens makes them an excellent reagent for ultrasensitive in situ hybridization detection of specific DNA sequences both with and without in situ PCR. Added on: 07-Oct-2004 Hits: 729 Rate this Site
Nanogold DNA In Situ Hybridization Description: This molecular morphological procedure is based on silver/gold-enhanced Nanogold (Nanoprobes, Inc.) and allowed a reliable and very sensitive detection of few copies of human papillomavirus (HPV), cytomegalovirus (CMV), Epstein Barr Virus (EBV), herpes simplex virus (HSV) and other DNA-viruses in routinely formalin-fixed and paraffin-embedded tissues. Other DNA sequences, as well as RNA stainings, have not yet been tested by us. The method described below can be applied as a robust and fast routine method for diagnostic purposes in all cases where "conventional ISH" is not sensitive enough, and where real single-copy sensitivity is not neded. The protocol also works well with Fluoro-Nanogold™ (Nanoprobes, Inc.), thus allowing visualization of the very same preparation by fluorescent microscopy (FISH) and transmitted light microscopy. Nanogold-preparations can be transferred to the electron microscope Added on: 16-Nov-2004 Hits: 712 Rate this Site
Nonradioactive In Situ Hybridization Application Manual Description: This manual concentrates on two labeling
systems:
-Indirect methods using digoxigenin (detected
by specific antibodies) and biotin
(detected by streptavidin)
-Direct methods using fluorescein or other
fluorochromes directly coupled to the nucleotide
PREPARATION OF CHROMOSOME SPREADS Description: An ideal method of tissue preparation ensures both good specimen morphology and that the target molecules are in the optimum state for probe access and hybridization. DNA:DNA in situ hybridization is usually carried out on chromosome spread preparations where chromosome and nuclei are released from cells and spread on a glass microscope slide. This method yields well separated and enlarged chromosomes with good morphology which can be analyzed in transmitted light or fluorescence microscopes. Added on: 17-Dec-2009 Hits: 252 Rate this Site
Single and Double FISH protocols for Drosophila Description: This procedure is taken from a recently published book chapter. The reference is: Hughes, S. and Krause, H.M. (1998) Single and double FISH protocols for Drosophila. in: Protocols in confocal microscopy., ed. Stephen Paddock, Humana Press Inc., in press. Added on: 30-Nov-2004 Hits: 705 Rate this Site
Two colour in situ hybridization Description: Two colour in situ hybridization - sequential alkaline phosphatase staining with chromogenic substrates
of zebrafish embryos. Protocols from Oxford Practical Approach Series. Added on: 08-Oct-2004 Hits: 1002 Rate this Site
Two colour ISH Description: Two colour ISH - sequential alkaline phosphatase staining with chromogenic substrates of chick,
mouse, and Xenopus embryos. Protocols from Oxford Practical Approach Series. Added on: 08-Oct-2004 Hits: 854 Rate this Site